LOCUS       NM_025873               2078 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus tRNA isopentenyltransferase 1 (Trit1), transcript
            variant 1, mRNA.
ACCESSION   NM_025873
VERSION     NM_025873.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Fradejas-Villar,N., Bohleber,S., Zhao,W., Reuter,U., Kotter,A.,
            Helm,M., Knoll,R., McFarland,R., Taylor,R.W., Mo,Y., Miyauchi,K.,
            Sakaguchi,Y., Suzuki,T. and Schweizer,U.
  TITLE     The Effect of tRNA[Ser]Sec Isopentenylation on Selenoprotein
            Expression
  JOURNAL   Int J Mol Sci 22 (21), 11454 (2021)
   PUBMED   34768885
  REMARK    GeneRIF: The Effect of tRNA([Ser]Sec) Isopentenylation on
            Selenoprotein Expression.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2078)
  AUTHORS   Cheong,A., Archambault,D., Degani,R., Iverson,E., Tremblay,K.D. and
            Mager,J.
  TITLE     Nuclear-encoded mitochondrial ribosomal proteins are required to
            initiate gastrulation
  JOURNAL   Development 147 (10) (2020)
   PUBMED   32376682
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2078)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 2078)
  AUTHORS   Fradejas,N., Carlson,B.A., Rijntjes,E., Becker,N.P., Tobe,R. and
            Schweizer,U.
  TITLE     Mammalian Trit1 is a tRNA([Ser]Sec)-isopentenyl transferase
            required for full selenoprotein expression
  JOURNAL   Biochem J 450 (2), 427-432 (2013)
   PUBMED   23289710
  REMARK    GeneRIF: Knockdown of Trit1 reduces expression of selenoproteins.
REFERENCE   6  (bases 1 to 2078)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   7  (bases 1 to 2078)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 2078)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL606906.18 and AK003556.1.
            
            On Sep 29, 2017 this sequence version replaced NM_025873.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK128921.1, BC019812.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 AL606906.18        158275-158278
            5-2063              AK003556.1         2-2060
            2064-2078           AL606906.18        196617-196631
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 57.36 cM"
     gene            1..2078
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /note="tRNA isopentenyltransferase 1"
                     /db_xref="GeneID:66966"
                     /db_xref="MGI:MGI:1914216"
     exon            1..191
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /inference="alignment:Splign:2.1.0"
     CDS             18..1421
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /EC_number="2.5.1.75"
                     /note="tRNA isopentenyltransferase, mitochondrial; tRNA
                     dimethylallyltransferase, mitochondrial; IPTase; IPP
                     transferase; isopentenyl-diphosphate:tRNA
                     isopentenyltransferase"
                     /codon_start=1
                     /product="tRNA dimethylallyltransferase precursor"
                     /protein_id="NP_080149.2"
                     /db_xref="CCDS:CCDS18607.1"
                     /db_xref="GeneID:66966"
                     /db_xref="MGI:MGI:1914216"
                     /translation="MAAAAAARAVPVSSGFRGLRRTLPLVVILGATGTGKSTLALQLG
                     QRLGGEIVSADSMQVYEGLDIITNKVSAQEQKMCQHHMISFVDPLVTSYTVVDFRNKA
                     TALIEDIFARDKIPIVVGGTNYYIESLLWKVLITTKPQEMGTGKVVDRKVELEKEDGH
                     ELHKRLSQVDPEMAAKLHPHDKRKVARSLQVFEETGISHSEFLHRQHAEEGGGPLGGP
                     LRFPNPCILWLHADQAVLDERLDKRVDDMLAAGLLEELRGFHRRYNLKNISENSQDYQ
                     HGIFQSIGFKEFHEYLTTEGKCTPETSNQLLKKGIEALKQVTKRYARKQNRWVKNRFL
                     SRPGPSVPPVYGLEVSDVSKWEESVLEPALNIVQSFIQGHKPTAMPVKMAYNESENKR
                     SYHMCDLCDRIIIGDREWAAHLKSKSHLHQLKKRRRLDLDAVSATGSQSNSPDCDPER
                     IEGESSGQHNQELKASV"
     exon            192..332
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /inference="alignment:Splign:2.1.0"
     exon            333..431
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /inference="alignment:Splign:2.1.0"
     exon            432..577
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /inference="alignment:Splign:2.1.0"
     exon            578..720
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /inference="alignment:Splign:2.1.0"
     exon            721..832
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /inference="alignment:Splign:2.1.0"
     exon            833..945
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1023
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1133
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1251
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..2078
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2044..2049
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /note="hexamer: AATAAA"
     polyA_site      2065
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /note="major polyA site"
ORIGIN      
        1 gctcagatgc cttcaagatg gcggctgcgg ctgctgcacg agcagttcct gtgagctctg
       61 gattccgagg cctgcggcga acactgccac ttgtagtgat tctcggggct actgggactg
      121 gcaagtccac cctggctctg cagttaggcc agcggctcgg cggcgagatc gtcagcgccg
      181 actccatgca ggtctatgaa gggctagaca tcatcaccaa taaggtctct gcccaagagc
      241 agaagatgtg ccagcaccac atgatcagct ttgtggatcc acttgtgacc agctacaccg
      301 tggtggactt taggaacaaa gcaacggccc tgattgaaga tatttttgct cgagacaaaa
      361 ttcccattgt ggtgggagga accaattatt acattgaatc tctgctctgg aaagttctta
      421 ttactaccaa gccccaggag atgggcactg ggaaagtggt tgatcggaaa gtagagcttg
      481 aaaaggaaga tggtcatgaa ctccataaac ggctaagcca ggtggaccca gaaatggccg
      541 ccaagctaca cccgcatgac aaacgcaaag tggccaggag cttgcaagtg tttgaagaaa
      601 caggaatctc tcacagtgaa ttccttcatc gtcagcacgc agaggaaggc ggtggtcctc
      661 ttggaggccc tctcaggttc cctaacccat gcatcctctg gctccatgct gaccaggcag
      721 ttctagatga gcgcttggat aaaagagtgg atgacatgct tgctgccgga ctcttggaag
      781 agctgagagg ttttcacaga cgttataatc ttaagaacat ttcagaaaat agccaggact
      841 atcaacacgg tatcttccag tcaattggct tcaaggaatt tcacgagtac ctgaccactg
      901 aggggaaatg cacaccagag actagtaacc agcttctaaa gaaaggtatt gaggctctga
      961 aacaagtaac taagagatat gcccggaaac agaaccgatg ggttaaaaac cgctttttga
     1021 gcagacctgg gcccagtgtc cccccggtat atggcttaga agtatctgat gtttccaagt
     1081 gggaggagtc tgttctggaa cctgctctca atatagtaca aagtttcatc cagggtcaca
     1141 aacctacagc catgccagtg aagatggcat acaatgaaag tgagaacaag agaagttacc
     1201 acatgtgtga cctctgtgac cggatcatca ttggggaccg ggaatgggca gcacatttaa
     1261 aatccaaatc tcacttgcac caattgaaga aaagaagaag gttggactta gacgctgtca
     1321 gtgccacagg aagtcaaagt aattccccag actgtgaccc ggaacgcatc gagggggaat
     1381 cctcggggca gcacaatcaa gagctgaagg ccagtgtttg agagacatgc ctagtggcct
     1441 ttgcagagac gtggggatca agcccaggag ggaggggagt gtcgctctcc cacgcctgga
     1501 ctgaggaatg ctgggcagaa ggccccacca tcttctttca ttctgtgctg tggtctgcag
     1561 tggaaacagc aggcctttca gctccttgtg tgcctgttgt gtctggtaat gatgtagttc
     1621 agagtgggat ttttttcttt gaaccttaaa ggttttattt tagaacgagg cacagatcgc
     1681 acattttcta cttgaggatc ttttttagtg gtgaatacca agattcagtg catcctttaa
     1741 aagagcgttc ttgtccctgg cgctggctaa aaatagctcg tttccagatg cttttgtaga
     1801 tgactgaagt attgtaaggc gcagtcagga gctctggact cgagaacggc agaaaggagt
     1861 agtgcagaga gatgattaag caaactctcc cagctctgtg aatctacaga agagggggtc
     1921 aggctgaggt ggttgtgacc tggatcttga agactaaaag acacagagtc tgctgagctg
     1981 ctcctgtgca tggtggctgt ctcttcatct agggcagaaa tctatagatt cttttgaaat
     2041 gtaaataaaa agattgtaaa cgccctatag tttctata
//
