LOCUS       NP_080149                467 aa            linear   ROD 02-MAY-2024
DEFINITION  tRNA dimethylallyltransferase precursor [Mus musculus].
ACCESSION   NP_080149
VERSION     NP_080149.2
DBSOURCE    REFSEQ: accession NM_025873.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 467)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 467)
  AUTHORS   Fradejas-Villar,N., Bohleber,S., Zhao,W., Reuter,U., Kotter,A.,
            Helm,M., Knoll,R., McFarland,R., Taylor,R.W., Mo,Y., Miyauchi,K.,
            Sakaguchi,Y., Suzuki,T. and Schweizer,U.
  TITLE     The Effect of tRNA[Ser]Sec Isopentenylation on Selenoprotein
            Expression
  JOURNAL   Int J Mol Sci 22 (21), 11454 (2021)
   PUBMED   34768885
  REMARK    GeneRIF: The Effect of tRNA([Ser]Sec) Isopentenylation on
            Selenoprotein Expression.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 467)
  AUTHORS   Cheong,A., Archambault,D., Degani,R., Iverson,E., Tremblay,K.D. and
            Mager,J.
  TITLE     Nuclear-encoded mitochondrial ribosomal proteins are required to
            initiate gastrulation
  JOURNAL   Development 147 (10) (2020)
   PUBMED   32376682
  REMARK    Publication Status: Online-Only
REFERENCE   4  (residues 1 to 467)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (residues 1 to 467)
  AUTHORS   Fradejas,N., Carlson,B.A., Rijntjes,E., Becker,N.P., Tobe,R. and
            Schweizer,U.
  TITLE     Mammalian Trit1 is a tRNA([Ser]Sec)-isopentenyl transferase
            required for full selenoprotein expression
  JOURNAL   Biochem J 450 (2), 427-432 (2013)
   PUBMED   23289710
  REMARK    GeneRIF: Knockdown of Trit1 reduces expression of selenoproteins.
REFERENCE   6  (residues 1 to 467)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   7  (residues 1 to 467)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (residues 1 to 467)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL606906.18 and AK003556.1.
            
            On Dec 18, 2002 this sequence version replaced NP_080149.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK128921.1, BC019812.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 57.36 cM"
     Protein         1..467
                     /product="tRNA dimethylallyltransferase precursor"
                     /EC_number="2.5.1.75"
                     /note="tRNA isopentenyltransferase, mitochondrial; tRNA
                     dimethylallyltransferase, mitochondrial; IPTase; IPP
                     transferase; isopentenyl-diphosphate:tRNA
                     isopentenyltransferase"
                     /calculated_mol_wt=47736
     transit_peptide 1..47
                     /note="Mitochondrion. /evidence=ECO:0000255; propagated
                     from UniProtKB/Swiss-Prot (Q80UN9.2)"
                     /calculated_mol_wt=4719
     Region          24..373
                     /region_name="MiaA"
                     /note="tRNA A37 N6-isopentenylltransferase MiaA
                     [Translation, ribosomal structure and biogenesis];
                     COG0324"
                     /db_xref="CDD:440093"
     mat_peptide     48..467
                     /product="tRNA dimethylallyltransferase.
                     /id=PRO_0000019024"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80UN9.2)"
                     /calculated_mol_wt=47736
     Region          55..58
                     /region_name="Interaction with substrate tRNA.
                     /evidence=ECO:0000250|UniProtKB:P07884"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80UN9.2)"
     Site            122
                     /site_type="other"
                     /note="Interaction with substrate tRNA.
                     /evidence=ECO:0000250|UniProtKB:P07884; propagated from
                     UniProtKB/Swiss-Prot (Q80UN9.2)"
     Region          183..187
                     /region_name="Interaction with substrate tRNA.
                     /evidence=ECO:0000250|UniProtKB:P07884"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80UN9.2)"
     Site            206
                     /site_type="other"
                     /note="Interaction with substrate tRNA.
                     /evidence=ECO:0000250|UniProtKB:P07884; propagated from
                     UniProtKB/Swiss-Prot (Q80UN9.2)"
     Region          221..230
                     /region_name="Core aggregation region.
                     /evidence=ECO:0000250|UniProtKB:P07884"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80UN9.2)"
     Region          233..255
                     /region_name="Interaction with isopentenylpyrophosphate
                     transferase. /evidence=ECO:0000250|UniProtKB:P07884"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80UN9.2)"
     Region          281..283
                     /region_name="Interaction with substrate tRNA.
                     /evidence=ECO:0000250|UniProtKB:P07884"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80UN9.2)"
     Region          313..331
                     /region_name="Interaction with substrate tRNA.
                     /evidence=ECO:0000250|UniProtKB:P07884"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80UN9.2)"
     Region          395..419
                     /region_name="zf-C2H2_jaz"
                     /note="Zinc-finger double-stranded RNA-binding; pfam12171"
                     /db_xref="CDD:432381"
     Region          432..467
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80UN9.2)"
     Site            443
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9H3H1; propagated from
                     UniProtKB/Swiss-Prot (Q80UN9.2)"
     Site            455
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9H3H1; propagated from
                     UniProtKB/Swiss-Prot (Q80UN9.2)"
     CDS             1..467
                     /gene="Trit1"
                     /gene_synonym="2310075G14Rik; IPPT; IPT; MOD5"
                     /coded_by="NM_025873.3:18..1421"
                     /db_xref="CCDS:CCDS18607.1"
                     /db_xref="GeneID:66966"
                     /db_xref="MGI:MGI:1914216"
ORIGIN      
        1 maaaaaarav pvssgfrglr rtlplvvilg atgtgkstla lqlgqrlgge ivsadsmqvy
       61 egldiitnkv saqeqkmcqh hmisfvdplv tsytvvdfrn kataliedif ardkipivvg
      121 gtnyyiesll wkvlittkpq emgtgkvvdr kvelekedgh elhkrlsqvd pemaaklhph
      181 dkrkvarslq vfeetgishs eflhrqhaee gggplggplr fpnpcilwlh adqavlderl
      241 dkrvddmlaa glleelrgfh rrynlknise nsqdyqhgif qsigfkefhe ylttegkctp
      301 etsnqllkkg iealkqvtkr yarkqnrwvk nrflsrpgps vppvyglevs dvskweesvl
      361 epalnivqsf iqghkptamp vkmaynesen krsyhmcdlc driiigdrew aahlkskshl
      421 hqlkkrrrld ldavsatgsq snspdcdper iegessgqhn qelkasv
//
