LOCUS       NM_001039156            7352 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus TRIO and F-actin binding protein (Triobp), transcript
            variant 3, mRNA.
ACCESSION   NM_001039156
VERSION     NM_001039156.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   Akturk,A., Day,M. and Tarchini,B.
  TITLE     RGS12 polarizes the GPSM2-GNAI complex to organize and elongate
            stereocilia in sensory hair cells
  JOURNAL   Sci Adv 8 (42), eabq2826 (2022)
   PUBMED   36260679
REFERENCE   3  (bases 1 to 7352)
  AUTHORS   Babahosseini,H., Belyantseva,I.A., Yousaf,R., Tona,R., Hadi,S.,
            Inagaki,S., Wilson,E., Kitajiri,S.I., Frolenkov,G.I., Friedman,T.B.
            and Cartagena-Rivera,A.X.
  TITLE     Unbalanced bidirectional radial stiffness gradients within the
            organ of Corti promoted by TRIOBP
  JOURNAL   Proc Natl Acad Sci U S A 119 (26), e2115190119 (2022)
   PUBMED   35737845
  REMARK    GeneRIF: Unbalanced bidirectional radial stiffness gradients within
            the organ of Corti promoted by TRIOBP.
REFERENCE   4  (bases 1 to 7352)
  AUTHORS   Krey,J.F., Liu,C., Belyantseva,I.A., Bateschell,M., Dumont,R.A.,
            Goldsmith,J., Chatterjee,P., Morrill,R.S., Fedorov,L.M., Foster,S.,
            Kim,J., Nuttall,A.L., Jones,S.M., Choi,D., Friedman,T.B.,
            Ricci,A.J., Zhao,B. and Barr-Gillespie,P.G.
  TITLE     ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points
  JOURNAL   J Cell Biol 221 (4) (2022)
   PUBMED   35175278
  REMARK    GeneRIF: ANKRD24 organizes TRIOBP to reinforce stereocilia
            insertion points.
REFERENCE   5  (bases 1 to 7352)
  AUTHORS   Katsuno,T., Belyantseva,I.A., Cartagena-Rivera,A.X., Ohta,K.,
            Crump,S.M., Petralia,R.S., Ono,K., Tona,R., Imtiaz,A., Rehman,A.,
            Kiyonari,H., Kaneko,M., Wang,Y.X., Abe,T., Ikeya,M.,
            Fenollar-Ferrer,C., Riordan,G.P., Wilson,E.A., Fitzgerald,T.S.,
            Segawa,K., Omori,K., Ito,J., Frolenkov,G.I., Friedman,T.B. and
            Kitajiri,S.I.
  TITLE     TRIOBP-5 sculpts stereocilia rootlets and stiffens supporting cells
            enabling hearing
  JOURNAL   JCI Insight 4 (12), 128561 (2019)
   PUBMED   31217345
  REMARK    GeneRIF: TRIOBP-5 sculpts stereocilia rootlets and stiffens
            supporting cells enabling hearing.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7352)
  AUTHORS   Riazuddin,S., Khan,S.N., Ahmed,Z.M., Ghosh,M., Caution,K.,
            Nazli,S., Kabra,M., Zafar,A.U., Chen,K., Naz,S., Antonellis,A.,
            Pavan,W.J., Green,E.D., Wilcox,E.R., Friedman,P.L., Morell,R.J.,
            Riazuddin,S. and Friedman,T.B.
  TITLE     Mutations in TRIOBP, which encodes a putative
            cytoskeletal-organizing protein, are associated with nonsyndromic
            recessive deafness
  JOURNAL   Am J Hum Genet 78 (1), 137-143 (2006)
   PUBMED   16385457
REFERENCE   7  (bases 1 to 7352)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   8  (bases 1 to 7352)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 7352)
  AUTHORS   Seipel,K., O'Brien,S.P., Iannotti,E., Medley,Q.G. and Streuli,M.
  TITLE     Tara, a novel F-actin binding protein, associates with the Trio
            guanine nucleotide exchange factor and regulates actin cytoskeletal
            organization
  JOURNAL   J Cell Sci 114 (Pt 2), 389-399 (2001)
   PUBMED   11148140
REFERENCE   10 (bases 1 to 7352)
  AUTHORS   Southard-Smith,E.M., Collins,J.E., Ellison,J.S., Smith,K.J.,
            Baxevanis,A.D., Touchman,J.W., Green,E.D., Dunham,I. and Pavan,W.J.
  TITLE     Comparative analyses of the Dominant megacolon-SOX10 genomic
            interval in mouse and human
  JOURNAL   Mamm Genome 10 (7), 744-749 (1999)
   PUBMED   10384052
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DQ228000.1, BP768417.1 and CX223548.1.
            
            Summary: This gene encodes a protein that interacts with trio,
            which is involved with neural tissue development and controlling
            actin cytoskeleton organization, cell motility, and cell growth.
            The encoded protein also associates with F-actin and stabilizes
            F-actin structures. Domains contained in this encoded protein are
            an N-terminal pleckstrin homology domain and a C-terminal
            coiled-coil region. Mutations in the human gene have been
            associated with a form of autosomal recessive nonsyndromic
            deafness. Multiple alternatively spliced transcript variants have
            been described [provided by RefSeq, Sep 2012].
            
            Transcript Variant: This variant (3) encodes the longest protein
            (isoform 3).
            
            Sequence Note: This RefSeq record was created from transcript
            sequence data from multiple strains because no single transcript
            from the same strain was available for the full length of the gene.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ228000.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164136
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7023              DQ228000.1         1-7023
            7024-7320           BP768417.1         47-343              c
            7321-7352           CX223548.1         218-249
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 37.7 cM"
     gene            1..7352
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /note="TRIO and F-actin binding protein"
                     /db_xref="GeneID:110253"
                     /db_xref="MGI:MGI:1349410"
     exon            1..325
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            326..483
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            484..635
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     CDS             613..6657
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /note="isoform 3 is encoded by transcript variant 3;
                     trio-associated repeat on actin; protein Tara"
                     /codon_start=1
                     /product="TRIO and F-actin-binding protein isoform 3"
                     /protein_id="NP_001034245.1"
                     /db_xref="CCDS:CCDS37136.1"
                     /db_xref="GeneID:110253"
                     /db_xref="MGI:MGI:1349410"
                     /translation="MSMEQDTRALLPTQGTAWATASAPVARLQGPQGDSHQACSQEPH
                     SPSSAEAPYCDLPRCPPALQNPLRTTTCVGQSVHSLGLGLGQEPQRVWSPTTALPAEG
                     PAAAPKNRHQDSEGIPYLEGLARSSCTDDNDNKDEDEDPNSNTSSSQDSNTPHDTSNS
                     SSVDWDTTERPGVVPSRNRLTEMIPRRPQEGLRADSARKATRSPARGDTAGQRKENSG
                     SGGQSAGQHWAKLRSESGYFSLERQRSGQTQASSGTPPSGPRGTTQASSAQRDVFQAA
                     PAQEAPQTSSLPRNTQRDTQRSTPRTSSPSRVSQRDTPRVMSTQRKNTPLSSPLRATP
                     ETLKISAPEDGTHVTPSPCVQDSSLNRTSQRDSSRTPCIQWDNPRASSPNRTTQRDNP
                     RTPCTQRDNPRASSPNRTTQRDNPRTPCTQRDNPRASSPNRTTQRDNPRTPCAQRDNP
                     RAASPNRSTQRDSPRTPCAQRDNPRASSPNRTAQRDNPRTPCAQRDNPRTSCTSQNTP
                     RTPSTQADKTTASCSKWEHLRSACTQRDNPRTFSQGCTQKDNPGPPSPRRATQGSNSR
                     NPSPHRTNKDIPWASFPLRPTQSDSPRTSSPSRTKQNQVPWASISLRPTQGDKPQTSA
                     PTRLAHNDPPQQYSPSLATTSSSSHNPGHSSASRTSSPLHAAPRGAPQTSLESSQPPC
                     TVCIGHRDAPRASSPPRYFQYDPFPFFPDPRSSESESPHHEPPYMPPAVCIGHRDAPR
                     ATSPPRHTQFDPFPFLPDTSDADNESPQHDPPQFPPPVCIGYRDAPRASSPPRQFPEP
                     SFFQDLPRASTESLVPSTDSMHEPPHIPTPVCIGHRDAPSFSSPPRQAPEPSLFFQDP
                     PGTSMESLAPSIDSLHGCPLLPPQVCIGHRDAPRASSPPRHPPSDIGLLAPSPPPGSS
                     GSRGSAPPGETRHNLEREEYTMLADLPPPRRLAQRGPEPQAQGSNEGRTRSPGRAEVE
                     RLFGQERRKSEAPGAFQTRDEGRSQRPSQAQSQLRRQSSPAPSRQVTKPSAKQAEPTR
                     QSRTGPPHPKSPDKRPEGDRQLQRTSPPARTPARPPERKAQIERHLESGHTGPRQSLG
                     GWQSQERLSGPQSPNRHPEKSWGSQKEGPSLGGWPELEGPSLEGIWRGPPQEHREQWG
                     HSEAWEEPPSNGIQGAPPRGQGRLQELSRPHQPTPSSENSWAGPAECSCALQPEASTA
                     VGWRAEGTSPHQRSAERPPDLDWRDLLGLLRAPEDGAWTRLPRLDWEGLLELLQARLP
                     QKDPARHWHDPAKASGPEQGSSGTEDTLKTEPQTQPEGRAKATLANGHRPGQQSESPA
                     QLPSPACTSTQWPTTKVTSGPETSPLAALEQIDHLESHSPPDLEFQPEEPEASEPSRG
                     EDSRAVQKQADSADKRPAEGKAGSPLKGRLVTSWRMPGDRPALFNPYLLSLGVLRWQR
                     PDLLNFKKGWMSILDEPGEWKKHWFVLTDSSLKYYRDSTAEEADELDGEIDLRSCTDV
                     TEYAVQRNYGFQIHTKDAVYTLSAMTSGIRRNWIEALRKTVRPTSAPDVTKLSDCNKE
                     NTLHGYGTQKSSLKIGEQRTGSEVIGRGGPRKADGPRPSLDYVELSPLAPSSPQRMRT
                     LSRSTPERPTKQEDLERDLAQRSEERRKWFESTDGRTPETPSGDGSRRGLGAPLTDDQ
                     QSRLSEEIEKKWQELEKLPLRENKRVPLTALLNQAHNDRRGPTSDSHEALEKEVQSLR
                     AQLEAWRLRGEAPQNAPRLQEDSHIPPGYISQEACERSLAEMESSHQQVMEQLQRHHE
                     RELQRLQQEKEWLLAEETAATASAIEAMKKAYQEELSRELSKTRSLQQGPESLRKQHQ
                     LDMEALKQELQVLSERYSQKCLEIGALTRQAEEREHTLRRCQQEGQELLRHNQELHSH
                     LSEEIDRLRSFIASQGTGNSCGRSNERSSCELEVLLRVKENELQYLKKEVQCLRDELQ
                     VIQKDKRFTGKYQDVYVELNHIKTRSEREIEQLKEHLRLAMAALQEKEAVRNSLAE"
     exon            636..735
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            736..881
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            882..1101
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1258
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..3551
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            3552..3663
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            3664..4704
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            4705..4782
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            4783..4920
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            4921..4977
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            4978..5046
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            5047..5136
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            5137..5246
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            5247..5775
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            5776..5886
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            5887..6034
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            6035..6137
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            6138..6297
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            6298..6411
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            6412..6498
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            6499..6659
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     exon            6660..7327
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7305..7310
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /note="hexamer: AATAAA"
     polyA_site      7327
                     /gene="Triobp"
                     /gene_synonym="EST478828; mKIAA1662; Tara"
                     /note="major polyA site"
ORIGIN      
        1 agatgctggt agcgcgggcg aaacgtgctg tgcatcccgc gcggcaaggc gcctgggcac
       61 cgcccgcctc tgccccgccc ctagtcctcg gtgtgtccag tcgcacactg accatgcaaa
      121 tgaagctctt agggagagcc tattggagtg agggaggggc gtggtctatc acgcaagtcc
      181 tgggccgcgt tttgattcat tgttaaaata gaggctgtga ctctggcacc agttcctccg
      241 ccgcccggag agtggaaacc cgagtccagg tcagaacccc gcggctagcg gaactaagct
      301 ggctggaaag gggaggccag aggagggaag atttctgcca gttggctggg gggagtcgtg
      361 aaggaaactg gagtcataag gggcacacga agagcccccg tttagcttcc ctggaaaagg
      421 agctacgttc tctatccaag aggatgctct tctcggtggc tggaccagaa aagaacagcc
      481 caggccccac ctagagaacc agccactgtg gcttccctgc tatggaggaa gtggctatga
      541 atatcccaag tatacaacct gaagccagtg tatctgccaa gaaccactgt ctgagctgct
      601 gtcaccctgg ggatgtccat ggagcaagac accagagcac tgctgcccac ccagggcact
      661 gcctgggcta cagcctctgc cccagtcgcc agactccagg gtccccaggg agactcccac
      721 caggcctgct ctcaggagcc tcacagccct tccagtgctg aggcacccta ctgcgacctg
      781 cctcgctgcc caccggccct gcagaaccca ctccgcacca ccacctgtgt cggccagtct
      841 gtgcacagcc ttggtctcgg ccttgggcag gagcctcaga gggtctggtc cccaacaaca
      901 gctctccctg cagagggccc tgcagctgcc ccaaagaaca gacaccagga ctccgagggg
      961 ataccctatc tggagggcct ggcccgtagc agctgcactg acgacaacga caacaaggac
     1021 gaagacgagg accctaactc caacaccagc tccagccaag acagcaatac ccctcatgat
     1081 accagcaatt cctcttctgt ggattgggac actactgaga gaccaggggt ggtccccagc
     1141 aggaacagac tcacagagat gatcccaagg aggcctcagg agggactgag agctgacagt
     1201 gctcgaaagg ccaccaggtc cccagcccga ggagacacag caggccagag gaaggagaat
     1261 tctggcagtg gtggacagag cgctggccag cactgggcaa agctgcgatc agaaagcggc
     1321 tacttctctc tggagcgaca gcgctcaggg caaacccagg cttcctccgg gacaccaccg
     1381 agcggacctc ggggcaccac ccaggcttct tctgcccaaa gggatgtttt ccaggctgct
     1441 cctgcacaag aagctcccca aacctcctct ctgccccgaa acacccaaag ggacacacag
     1501 cggagcaccc ctagaacctc ctctccttcc agagtatccc agcgggacac tcccagagtc
     1561 atgtccaccc agcggaagaa tactcctctt tcctctcccc tcagagccac tccagagacc
     1621 ctaaagatct ctgcaccaga agatggcacc catgttacac catctccatg tgtccaagac
     1681 tcctctctca acagaaccag ccagcgggac agctctagaa ctccctgtat ccagtgggac
     1741 aatcccaggg cctcctctcc caacagaact actcagcggg acaaccctag gactccctgt
     1801 acccagaggg acaatcccag ggcctcctct cccaacagaa ctactcagcg ggacaaccct
     1861 aggactccct gtacccagag ggacaatccc agggcctcct ctcccaacag aactactcag
     1921 agggacaacc ccaggactcc ctgtgcccag agggacaatc ccagggctgc ttctcccaac
     1981 agaagtactc agcgggacag ccccaggact ccctgtgccc aacgggacaa tcccagggcc
     2041 tcctctccca acagaactgc ccagcgagac aaccccagga ctccctgtgc ccagcgggac
     2101 aaccccagga cctcctgcac atcacagaat acccccagga ccccttctac ccaagcagat
     2161 aagactacag cttcctgcag caaatgggag catctgcgaa gcgcctgcac ccagcgagac
     2221 aaccccagga ccttctctca aggctgcacc caaaaggaca atccaggacc accctcgccc
     2281 cgccgtgcca ctcaggggag taattccagg aacccttccc cccaccgtac caacaaagat
     2341 atcccttggg cttcctttcc ccttcggcca acacagagtg acagtccccg aacctcatct
     2401 ccatcgcgca ccaaacaaaa ccaggtgcct tgggcatcca tttctctccg gccaacccaa
     2461 ggggacaagc ctcagacctc agctcctacc aggctagctc acaatgaccc tccccagcag
     2521 tattcgccat ccctggccac cacctcctcc tcctctcata accctggcca ttccagtgcc
     2581 tctaggactt catcgcctct tcacgcagca cctcgagggg ctccccagac ctctttggag
     2641 tcctctcagc caccctgcac tgtgtgcatc gggcaccggg acgcacctcg cgcctcttcc
     2701 ccacctcgct attttcagta cgaccctttc cccttcttcc cagatccccg ctcgtctgag
     2761 agtgaatcgc cccaccacga acccccctac atgccgcctg cagtgtgtat tgggcaccgt
     2821 gatgcccccc gggccacttc gccacctcgt cacacccagt tcgatccctt ccccttcctc
     2881 ccagacacat cagatgctga taatgagtcc ccgcagcacg acccccctca gtttccccca
     2941 ccagtgtgca tcgggtaccg cgacgctccg cgggcctctt ccccaccacg ccagttccca
     3001 gagccctcct tcttccaaga tctccccagg gccagcacag agagccttgt cccttccaca
     3061 gactctatgc atgagccgcc ccacattccc actcctgtat gtatcggaca cagagacgct
     3121 ccctccttct cctccccgcc acgccaggcc cccgagcctt ccctcttctt ccaggatcct
     3181 cctgggacca gtatggagag tcttgctcct tccattgact ctctgcatgg ctgcccactg
     3241 ctgccccccc aagtttgcat cggccatcgg gatgcaccca gagcctcctc tcctccccgc
     3301 cacccaccta gtgacatagg tctcctggca ccctcacctc caccaggtag ctcgggctcc
     3361 cggggctcag cacccccagg ggagaccaga cacaacttgg agagggagga gtacaccatg
     3421 ctggctgacc tgcccccacc caggaggctg gctcagaggg ggccagagcc ccaggcacag
     3481 ggcagcaacg agggccgtac ccgcagccct ggccgagcag aggtggagcg cctcttcggg
     3541 caagagcgca ggaaatcgga ggcaccgggg gccttccaga ctcgggatga gggacggtca
     3601 cagcggccca gccaagctca gagccaactt cgaagacagt ccagccctgc ccccagcagg
     3661 caggtgacca agccctctgc caagcaggca gagccaaccc ggcagagccg aacaggaccg
     3721 ccacatccta agagtcctga caagcggcct gagggagacc ggcagctcca gaggacctca
     3781 ccacctgcca ggacaccagc caggccccct gagagaaagg ctcagataga gaggcatctg
     3841 gagagtggcc acactggccc gaggcagtct ctgggagggt ggcagagcca ggagaggctg
     3901 tcaggacctc aaagtcctaa cagacaccca gagaagagct ggggcagcca gaaagagggc
     3961 ccaagcctgg ggggttggcc ggaacttgag ggtcccagcc tggaggggat atggaggggt
     4021 cctccccagg agcacaggga acagtgggga cattcagagg cctgggagga gcctcccagt
     4081 aatgggatac agggggctcc cccaaggggc caaggtagac ttcaggagct gtccagaccc
     4141 caccagccta cgccttcctc agagaactcc tgggcaggcc cagcagagtg ttcgtgtgcc
     4201 ctgcagcctg aggccagcac tgctgtgggc tggagggccg agggaacatc cccacatcag
     4261 cgtagtgctg agaggccacc cgacctagat tggagggacc tgctaggcct tctccgggca
     4321 cctgaggatg gggcctggac ccggctccca aggctggact gggaaggcct cctggagctc
     4381 ctgcaggctc ggctgcccca aaaggacccg gctaggcact ggcatgaccc agccaaggct
     4441 tcaggaccag agcagggttc ctcaggcaca gaggataccc tgaagacaga gccgcagacc
     4501 cagcctgaag gacgggccaa ggccacccta gccaatggac acaggcctgg tcagcagtct
     4561 gagagcccag cacagctacc cagccctgcc tgtacctcca cccagtggcc aaccaccaaa
     4621 gtgacaagcg gaccagaaac gtcacctctg gctgctctgg agcagataga tcacctagag
     4681 agccacagcc caccggacct ggagttccag ccagaggaac ctgaggcatc agaaccaagc
     4741 cgaggcgaag actcacgggc tgtccagaag caggctgact cggcagacaa gaggccggca
     4801 gagggcaagg ctgggagccc actcaagggc cgactggtga cttcatggcg gatgcccggg
     4861 gaccggcccg cgctgttcaa tccatacctg ctgtctctgg gggtcctcag gtggcaaagg
     4921 cccgatctgc tcaacttcaa gaaaggatgg atgtcaatct tggatgagcc tggagagtgg
     4981 aaaaagcatt ggtttgtgct gacggattca agcctcaaat attacagaga ctccacggct
     5041 gaggaggcag atgagctgga cggtgagatc gacctgcggt cctgtactga cgtcacagag
     5101 tatgcggtcc agcgcaacta tggcttccag atccacacca aggacgccgt ttataccctg
     5161 tcggccatga cctcgggcat ccggcggaac tggattgagg ctctcaggaa aactgtgcgg
     5221 ccaacttcag ccccagatgt caccaagctc tccgactgca ataaagaaaa cacgctgcat
     5281 ggctatggca cccagaagag ctccctgaag ataggggagc agcggacagg ctctgaggtt
     5341 atcggccggg gtggtcctcg caaggcggat ggaccacggc cgtcactgga ctacgtggag
     5401 ctctctccat tagcgccaag ctccccacag cgcatgcgca ctctctcccg ctccactccc
     5461 gagcgcccca ccaagcagga ggatctggag cgggacttgg cccaacgctc tgaggaaaga
     5521 cgcaagtggt tcgagtccac ggacggcagg accccagaga cgcccagtgg cgatgggtct
     5581 cgaaggggcc tgggtgcccc cctgactgat gaccagcaga gcaggctcag tgaggagatt
     5641 gagaagaagt ggcaggagtt ggaaaagctg cccctgcggg agaacaagcg agtgccactc
     5701 accgccctgc tcaaccaagc ccacaacgat cgtcggggac ctacaagtga cagccatgag
     5761 gccctggaga aggaggtcca gtctctccgt gcccagctgg aggcttggcg tctccgaggg
     5821 gaagctcctc agaatgcacc gagactccag gaggacagcc atatcccccc aggctacatc
     5881 tcgcaggaag cctgtgagcg cagcctggca gagatggagt cctcacacca gcaggtgatg
     5941 gagcagctgc agcggcacca tgagcgagag ctgcagcggc tgcagcagga gaaggagtgg
     6001 ctcctggccg aggagacggc tgccacggcc tcagctattg aagccatgaa gaaggcgtat
     6061 caagaagagc taagccggga gctgagcaaa acccggagtc tccagcaggg cccagagagc
     6121 cttcggaagc aacaccagct ggacatggag gcgctaaagc aggagctgca ggtgctatct
     6181 gagaggtact cgcagaagtg cctggagatc ggggccctga caaggcaggc agaagagcgt
     6241 gagcacaccc tgcggcgctg ccagcaagag ggccaggagc tgctgcgcca caaccaggag
     6301 ctgcactccc acctgtccga ggagatcgac cgtctgcgca gcttcatcgc ctcgcagggc
     6361 acaggcaaca gctgcgggcg cagcaatgag aggagttcct gtgagctgga ggtgctgctg
     6421 cgagtgaagg agaatgaact ccagtacctg aagaaggagg tgcagtgtct ccgagatgag
     6481 ctccaggtga tacagaagga caagcgcttc acggggaagt accaggatgt gtacgtggag
     6541 ctgaaccaca tcaagactcg gtcggagcgt gagatcgagc agctgaagga gcacctgcgc
     6601 ctggccatgg ctgccctgca ggagaaggag gctgtgagga acagcttggc tgagtagagg
     6661 tccctgtggt tccgatcagc tgaggaccct ccagccccag gggaccagcc accctccttc
     6721 tctggcagaa gtcagaagcc aagctttctc cccaccctgg gtcacatgtg cactcacacc
     6781 acacacaccc acacacacac gcacacatac acacacacac acacacacac gcatatgcac
     6841 acacatgcac acacacatac acgcacacac atgcacagac actgcggatc tgagctcgtc
     6901 ccttgcacag ggtctttacc ttgcaccttc ttcaggattt tatatgtgaa gagattttta
     6961 tatagatttt tttttttgcc tttttttttt tctaaaacat ttaaactttt taaaaaggca
     7021 atttcttggt ggcgtgtgcc tcaagcactg gcttctctcc ctcttgtccc ctgcttggcc
     7081 ctccttccta cctgggtgtc tcacaaaggc ccaaggagag ccagggtgag tcaaccagag
     7141 ggaaaaggca gccctcagtt ccctgccctg gcctctgggc agagggagga gccagtggct
     7201 ggccccatat ccactcctct gaggggcgct gggcgtgggc caccagcgcc accagcgcca
     7261 cccatgaaga cacttctctt ctgttccgtg agtcacccta acttaataaa accttccggc
     7321 aactctgaaa aaaaaaaaaa aaaaaaaaaa aa
//
