LOCUS       NM_001081302           11521 bp    mRNA    linear   ROD 31-DEC-2024
DEFINITION  Mus musculus triple functional domain (PTPRF interacting) (Trio),
            mRNA.
ACCESSION   NM_001081302 XM_888341
VERSION     NM_001081302.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Eid,L., Lokmane,L., Raju,P.K., Tene Tadoum,S.B., Jiang,X.,
            Toulouse,K., Lupien-Meilleur,A., Charron-Ligez,F., Toumi,A.,
            Backer,S., Lachance,M., Lavertu-Jolin,M., Montseny,M.,
            Lacaille,J.C., Bloch-Gallego,E. and Rossignol,E.
  TITLE     Both GEF domains of the autism and developmental epileptic
            encephalopathy-associated Trio protein are required for proper
            tangential migration of GABAergic interneurons
  JOURNAL   Mol Psychiatry (2024) In press
   PUBMED   39300136
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 11521)
  AUTHORS   Wang,X., Zhang,Y., Yu,J., Ma,Y., Xu,Y., Shi,J., Qi,Z. and Liu,X.
  TITLE     Identification and analysis of key circRNAs in the mouse embryonic
            ovary provides insight into primordial follicle development
  JOURNAL   BMC Genomics 25 (1), 139 (2024)
   PUBMED   38310234
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 11521)
  AUTHORS   Sun,X., Wang,L., Wei,C., Sun,M., Li,Q., Meng,H., Yue,W., Zhang,D.
            and Li,J.
  TITLE     Dysfunction of Trio GEF1 involves in excitatory/inhibitory
            imbalance and autism-like behaviors through regulation of
            interneuron migration
  JOURNAL   Mol Psychiatry 26 (12), 7621-7640 (2021)
   PUBMED   33963279
REFERENCE   5  (bases 1 to 11521)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 11521)
  AUTHORS   Charrasse,S., Comunale,F., Fortier,M., Portales-Casamar,E.,
            Debant,A. and Gauthier-Rouviere,C.
  TITLE     M-cadherin activates Rac1 GTPase through the Rho-GEF trio during
            myoblast fusion
  JOURNAL   Mol Biol Cell 18 (5), 1734-1743 (2007)
   PUBMED   17332503
  REMARK    GeneRIF: M-cadherin-dependent adhesion can activate Rac1 via the
            Rho-GEF Trio at the time of myoblast fusion.
REFERENCE   7  (bases 1 to 11521)
  AUTHORS   Sun,Y.J., Nishikawa,K., Yuda,H., Wang,Y.L., Osaka,H., Fukazawa,N.,
            Naito,A., Kudo,Y., Wada,K. and Aoki,S.
  TITLE     Solo/Trio8, a membrane-associated short isoform of Trio, modulates
            endosome dynamics and neurite elongation
  JOURNAL   Mol Cell Biol 26 (18), 6923-6935 (2006)
   PUBMED   16943433
REFERENCE   8  (bases 1 to 11521)
  AUTHORS   Dalkilic,I., Schienda,J., Thompson,T.G. and Kunkel,L.M.
  TITLE     Loss of FilaminC (FLNc) results in severe defects in myogenesis and
            myotube structure
  JOURNAL   Mol Cell Biol 26 (17), 6522-6534 (2006)
   PUBMED   16914736
REFERENCE   9  (bases 1 to 11521)
  AUTHORS   McPherson,C.E., Eipper,B.A. and Mains,R.E.
  TITLE     Multiple novel isoforms of Trio are expressed in the developing rat
            brain
  JOURNAL   Gene 347 (1), 125-135 (2005)
   PUBMED   15715966
REFERENCE   10 (bases 1 to 11521)
  AUTHORS   O'Brien,S.P., Seipel,K., Medley,Q.G., Bronson,R., Segal,R. and
            Streuli,M.
  TITLE     Skeletal muscle deformity and neuronal disorder in Trio exchange
            factor-deficient mouse embryos
  JOURNAL   Proc Natl Acad Sci U S A 97 (22), 12074-12078 (2000)
   PUBMED   11050238
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY727751.1, AB106872.1, CF539743.1, BC060724.1 and BC051169.1.
            
            On Apr 21, 2007 this sequence version replaced XM_888341.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-564               BY727751.1         1-564
            565-3761            AB106872.1         36-3232
            3762-4257           CF539743.1         32-527
            4258-7996           BC060724.1         474-4212
            7997-11521          BC051169.1         1464-4988
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 10.4 cM"
     gene            1..11521
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /note="triple functional domain (PTPRF interacting)"
                     /db_xref="GeneID:223435"
                     /db_xref="MGI:MGI:1927230"
     exon            1..509
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     CDS             353..9664
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /EC_number="2.7.11.1"
                     /codon_start=1
                     /product="triple functional domain protein"
                     /protein_id="NP_001074771.1"
                     /db_xref="CCDS:CCDS49587.1"
                     /db_xref="GeneID:223435"
                     /db_xref="MGI:MGI:1927230"
                     /translation="MSGSSGGATAPAASSGPAAAASAAGSGCGGGAGEGAEEAAKDLA
                     DIAAFFRSGFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQE
                     DLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHIALIIK
                     PDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRV
                     AFEEYISNAAHMLSRLEELQDVLAKKELPQDLEGARNMIDEHSQLKKKVIKAPIEDLD
                     LEGQKLLQRIQSSDSFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKL
                     KLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCM
                     NVYVNINRIMSVANRLVESGHYASQQIKQIANQLEQEWKAFAAALDERSTLLDMSSIF
                     HQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSL
                     LDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRL
                     QLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNA
                     DKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKE
                     LWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRD
                     SAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDI
                     ISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYV
                     NEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQA
                     EVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAE
                     AMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESL
                     EQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYL
                     HRNSVSMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERS
                     AKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLAD
                     GFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLD
                     IIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLW
                     EMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWAD
                     KFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLL
                     KELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQV
                     WDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDP
                     CKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTVHLRGALKEPIHIPKT
                     APAARQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFT
                     ACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSME
                     MEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRL
                     SSGKADGHAKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETIEERGRNEGLSSG
                     TLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTS
                     SETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDE
                     MEERKCSSLKRRHYVLQELVETERDYVRDLGCVVEGYMALMKEDGVPDDMKGKDKIVF
                     GNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIVSE
                     YIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELEKAV
                     EVMCIVPKRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCKERRVFL
                     FEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVESDPCKFALTSRTGDAVET
                     FVLHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGSGSGGSSGGGG
                     GSGGSGASSGGSSSHGSGPSSCSSGPSSSRSRPSRIPQPVRHHPPMLVSSAASSQAEA
                     DKMSGMSAPSPSLPTPSSSLALEASLGQPSRLPLSGDSEGHERETEPIPKMKVMESPR
                     KAPGSTSGTSQDGNTKDARGNLGSLPLGKTRPGAVSPLNSPLSTTFPSPFGKEAFPPS
                     SPLQKGGSFWSSIPASPASRPSSFTFPGDSDSLQRQTHRHAAPSKDTDRMSTCSSASE
                     QSVQSTQSNGSEGSSSSNISTMLVTHEYTAVKEDEINVYQGEVVQILASNQQNMFLVF
                     RAATDQCPAAEGWIPGFVLGHTSAVIMENPDGTLKKSTSWHTALRLRKKSEKKDKDGK
                     RDGKLENGYRKPREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVFRCRV
                     CGRPKASITWKGPEHNTLNNDDHYSISYSDIGEATLKIIGVSTEDDGIYTCIAVNDMG
                     SASSSASLRVLGPGSDGIVVTWKDNFDAFYSEVAELGRGRFAVVKKCDQKGTKRAVAT
                     KFVNKKLMKRDQVTHELGILQNLQHPLLVSLLDTFETPTSYVLVLEMADQGRLLDCVV
                     RWGSLTEGKVRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDQSLAKPTIKLADFGDA
                     VQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTADTWSVGVLTYVLLSGVSPFLDDSVE
                     ETCLNICRLDFSFPEDYFQGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGS
                     GKGTGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV"
     exon            510..584
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            585..699
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            700..892
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1405
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1528
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1720
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            1721..1852
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            1853..2083
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..2206
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            2207..2398
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            2399..2568
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            2569..2743
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            2744..2939
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            2940..3106
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            3107..3226
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            3227..3418
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            3419..3568
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            3569..3683
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            3684..3799
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            3800..3922
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            3923..4117
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            4118..4233
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            4234..4300
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            4301..4410
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            4411..4480
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            4481..4570
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            4571..4663
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            4664..4775
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            4776..4966
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            4967..5068
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            5069..5211
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            5212..5311
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            5312..5555
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            5556..5848
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            5849..6019
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            6020..6115
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            6116..6264
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            6265..6331
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            6332..6435
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            6436..6505
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            6506..6595
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            6596..6688
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            6689..6739
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            6740..6817
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            6818..7009
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            7010..7187
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            7188..7996
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            7997..8244
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            8245..8383
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            8384..8411
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            8412..8574
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            8575..8696
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            8697..8775
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            8776..8976
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            8977..9115
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            9116..11497
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11477..11482
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /note="hexamer: ATTAAA"
     polyA_site      11495
                     /gene="Trio"
                     /gene_synonym="6720464I07Rik; Solo"
                     /note="major polyA site"
ORIGIN      
        1 ggaaagcaag atggaggctc gctgctgccg ccggcgttcg tgatcgcgga gcttcgcagc
       61 cccggccccc gccctcgcgt gtccgcgccg ctgcccgtgc cccggccggc cacgccgagg
      121 ccgtggctcc gcgcgcccgc tccgcgccag gctcgcggct cccgggcggt gtcctcgtct
      181 atgtgggcgc gctcctcggg ccgagccgcc gccgcccgcc gccccggggc tgtgcgtccg
      241 cgcgccgggc gcgggcagct gggtgctcgg cgccgccagg ccaggcgcag gcggctgggt
      301 gcgcggagct aggggcgcgg ggcccgaggc gggctcgtcc gccgccgaag ccatgagcgg
      361 cagcagcggc ggtgccaccg ccccggccgc gtcctccggc cccgcggcag cggccagcgc
      421 ggcgggctcc ggctgcgggg gcggcgccgg cgagggcgcc gaggaggccg ccaaggacct
      481 agccgacatc gcggccttct tccgatccgg gtttcggaaa aatgatgaaa tgaaagctat
      541 ggatgttttg ccaattctga aggaaaaagt cgcctatctt tcaggtggta gagataagcg
      601 tggaggtccc attttaacat ttccagcccg cagcaatcat gacagaatac ggcaggagga
      661 cctcaggagg ctcatttcct atttagcctg cattcccagt gaggaagtct gcaagcgagg
      721 gttcacagtg attgtggaca tgcgcggctc caagtgggac tccatcaagc cgctgctgaa
      781 gatcctgcag gagtcctttc cgtgctgcat ccacatagcc ctgataatca agcccgacaa
      841 cttctggcag aaacagagga cgaattttgg cagctccaaa tttgaatttg agacaaatat
      901 ggtctcctta gaaggcctta ccaaagtagt tgatccttcc cagctaactc ccgagtttga
      961 cggctgcctg gaatacaacc atgaggagtg gatagaaatc cgagtagctt tcgaggagta
     1021 cattagcaat gcagcccata tgctgtcacg gctagaggaa cttcaggatg ttctcgccaa
     1081 aaaggagttg cctcaggacc tagagggggc tcggaacatg atcgacgagc actcccagtt
     1141 gaagaagaag gtgatcaagg cccccattga ggacttggac ttagaagggc agaagctgct
     1201 tcagcggata cagagcagcg acagtttccc caaaaagaac tcaggctcgg gcaatgcaga
     1261 cctgcagaac ctcctgccca aggtgtccac catgctagac aggctgcact ccacccggca
     1321 gcacctccac cagatgtggc acgtgaggaa actgaagctg gaccagtgct tccagctgcg
     1381 gctgttcgaa caggatgctg agaagatgtt tgactggatc actcacaaca aaggcctgtt
     1441 tctgaacagc tacacggaga tcgggaccag ccacccgcat gccatggaac ttcagacgca
     1501 gcacaatcat tttgccatga actgtatgaa cgtatatgta aatataaacc gcataatgtc
     1561 agtggccaat cgcctggtgg agtcgggcca ctacgcctct cagcagatta agcagattgc
     1621 aaatcagctg gaacaagagt ggaaggcgtt tgcagcagcc ttggacgagc gcagcacctt
     1681 gttggacatg tcctccatct ttcaccagaa ggctgagaag tacatgagca acgtggactc
     1741 gtggtgtaag gcctgtggtg aagtagacct tccctcggag ctgcaggacc tggaagacgc
     1801 cattcatcat caccagggca tctacgagca catcaccctc gcctactcag aggtgagcca
     1861 ggatgggaag tccctccttg ataagctcca gcgccccctg acccccggca gctccgactc
     1921 cctcaccgcc tccgccaact actccaaggc tgtgcaccac gtcttggatg tcatccacga
     1981 ggtcctgcac caccagcgcc agctggagaa catctggcag caccgcaagg ttcggctcca
     2041 ccagaggctg cagctctgcg tcttccagca ggacgttcag caggtgctgg actggataga
     2101 gaaccacgga gaagcgtttc tgagcaaaca cacgggcgtg ggcaaatccc ttcaccgggc
     2161 cagagctttg cagaaacgcc acgaggactt cgaggaagtc gcacagaaca cgtacacaaa
     2221 cgcagataaa ctgctagaag cagcagagca gctggctcag acgggggaat gtgaccccga
     2281 agagatttat caggctgccc atcagcttga agaccggata caagattttg ttcggcgagt
     2341 tgaacaacga aagatcctgc tggacatgtc tgtgtcattt cacacgcacg taaaagagct
     2401 ctggacatgg ctggaggagt tacagaagga actgctggac gacgtgtacg ccgagtcggt
     2461 ggaggccgtg caggacctca tcaagcgatt cggccagcag cagcagacca ccctgcaggt
     2521 gactgtcaac gtgatcaagg aaggagaaga cctcatccag cagctgaggg actccgccat
     2581 ctccagtaac aaaactccgc acaacagttc catcaaccac atcgagacgg tgctacagca
     2641 gctggacgag gctcagtccc agatggagga gctcttccag gagcgcaaga tcaagctgga
     2701 gctcttcctg cagctgcgca tcttcgagcg ggatgccatc gatatcatct cggaccttga
     2761 gtcgtggaat gatgagcttt cccagcaaat gaatgacttc gatacggagg acctgacgat
     2821 cgcagagcag cgcctacagc accatgcaga caaagccctg accatgaata acttgacttt
     2881 cgatgtcatc caccaaggac aggatcttct gcagtatgtc aatgaggtcc aggcctctgg
     2941 tgtggagctg ctttgtgaca gagatgtgga catggccact cgggttcagg acctcctgga
     3001 gtttcttcat gagaagcagc aggagttgga tttggcagct gagcagcatc ggaaacactt
     3061 ggagcagtgt gtgcagttgc gccacctgca ggcggaagtg aagcaggtgc tgggctggat
     3121 ccgaaatgga gagtccatgc taaatgctgg gctcatcacg gccagctcgc tgcaggaagc
     3181 cgagcagctg cagcgagaac acgagcagtt ccagcatgcg attgagaaaa cgcaccagag
     3241 cgcgctgcag gtacagcaga aggcggaggc catgctgcag gccaaccact atgacatgga
     3301 catgatccgg gactgtgcag agaaggtcgc ctcgcactgg cagcagctca tgctgaagat
     3361 ggaagaccgc ctcaagcttg tcaatgcctc tgtcgccttc tacaagactt ctgagcaggt
     3421 ctgcagtgtc ctggaaagtc tggaacagga gtacaagaga gaagaggact ggtgtggcgg
     3481 ggccgacaag ctgggcccca attctgagac tgaccacgtc acccccatga ttagcaagca
     3541 cctcgaacaa aaggaagcct tcctgaaggc ttgcacactg gccaggagga acgcagacgt
     3601 cttcctaaag tacctgcaca ggaacagcgt gagcatgccg gggatggtga cgcacatcaa
     3661 agctccggaa cagcaagtaa aaaatatctt gaatgaactc ttccagcggg aaaacagggt
     3721 attgcattat tggaccatga ggaaaaggcg gctggaccag tgtcagcagt acgtggtctt
     3781 cgagaggagc gccaagcagg cattagagtg gatccacgac aatggggagt tctacctttc
     3841 cacacatacc tcaacagggt ccagcattca gcacacacag gagctgttga aagaacacga
     3901 ggagtttcag ataactgcca agcaaaccaa agagagggtg aagctattga tacagctggc
     3961 cgatggcttt tgtgaaaaag ggcatgccca tgcagcagaa attaaaaaat gtgtgaccgc
     4021 agtggataag aggtacagag atttctctct gcgcatggag aagtaccgga cctctttgga
     4081 gaaagccctg gggatttctt cagattccaa caaatcgagt aaaagcctgc agttggacat
     4141 catccctgcc agtatccccg ggtcagaggt gaagcttcgg gatgctgccc acgagcttaa
     4201 tgaagagaag cggaagtctg cccgcaggaa agagttcatc atggctgaac taattcaaac
     4261 ggaaaaggct tatgtcagag acctccgaga gtgtatggat acctacctgt gggaaatgac
     4321 cagcggcgtg gaggagatcc cgccaggcat tgtcaacaag gagctcatca tcttcgggaa
     4381 catgcaggag atctacgagt tccacaacaa catcttcctg aaggagctgg agaagtacga
     4441 gcagctccca gaggacgtgg gacactgctt cgtcacctgg gcagacaagt ttcagatgta
     4501 tgtcacatac tgcaaaaata aacctgattc tacacagctg atactagagc acgcagggtc
     4561 ctactttgat gagatccagc agcggcatgg cttagccaat tccatctctt cctacctgat
     4621 caaaccagtt cagcggataa caaagtatca gctcctttta aaggagctgc tcacatgctg
     4681 tgaggaagga aagggggaga tcaaagatgg cctggaggtg atgctcagcg tgcccaagcg
     4741 agccaatgat gccatgcacc tcagcatgct ggaagggttt gacgaaaaca ttgagtctca
     4801 gggagagctc atcctgcagg agtccttcca agtgtgggac ccgaaaacct taattcggaa
     4861 gggtcgagaa cggcatctct tcctttttga aatgtccttg gtgtttagta aagaagtgaa
     4921 agactccagc gggagaagca agtacctgta caaaagcaag ctgtttacct cagagttggg
     4981 tgtcacagaa cacgttgaag gagatccttg caaatttgca ctgtgggtgg ggaggacgcc
     5041 aacttcagat aataaaattg tccttaaggc atccagtatc gagaacaagc aggactggat
     5101 caagcacatc cgtgaggtga tccaggagag gacggtgcac ctgagaggag ccctgaagga
     5161 gcccattcac atccccaaaa cagctccggc agcaaggcag aaggggagga gggatggaga
     5221 agacctggac agccaagggg atggcagcag ccagcccgac accatttcca tcgcctccag
     5281 gacatctcag aacacactgg acagtgataa gctttctggt ggctgcgagc tgacggtcgt
     5341 gattcatgac ttcacagcct gcaacagcaa tgagctgacc atccggcgtg gccagacagt
     5401 ggaggtcctg gaacggcctc acgacaagcc cgactggtgt ctggtgcgca ccactgacag
     5461 gtcccctgca gcagaaggcc tcgtgccctg tggctcactg tgcatcgccc actcccgaag
     5521 tagcatggag atggagggca tcttcaacca caaagactca ctgtcggtct ccagtaatga
     5581 cgccagccca ccggcctctg tggcttccct tcagccccac atgatcggag cacagagttc
     5641 accaggtccc aagcggcccg gcaacaccct gcgcaagtgg ctcacgagcc ccgtgcggcg
     5701 gctcagcagc ggtaaggccg atgggcatgc gaagaagctg gcccacaagc acaagaagag
     5761 ccgggaggtc cgtaagagcg cggatgctgg atcgcagaag gattcggatg acagcgctgc
     5821 caccccgcag gatgaaacca tcgaagagag aggacggaac gagggtctca gcagtggtac
     5881 gctgtccaag tcctcgtcct cgggcatgca gagctgcgga gaagaagaag gcgaggaggg
     5941 cgccgacgct gtaccactgc ctccccccat ggccattcag cagcacagcc tccttcagcc
     6001 ggactcgcag gatgacaagg cctcttcaag gttattggtc cgccccacca gttctgagac
     6061 tcccagcgca gctgagctcg tcagtgctat tgaagaactc gtgaaaagca aaatggcact
     6121 ggaggatcgt cccagctcgc tgctcgtcga ccagggagac agtagcagcc catccttcaa
     6181 tccctcagac aattccctcc tctcatcctc ctcacccatt gatgagatgg aagaaagaaa
     6241 atgcagctct ctgaagagaa ggcactatgt tttgcaagag ttagtggaga cagagcgaga
     6301 ctatgtacgg gaccttggct gtgtggttga gggctacatg gcacttatga aggaagacgg
     6361 cgttcctgat gacatgaagg gaaaagacaa gatcgtgttt ggcaacatcc atcagattta
     6421 tgactggcac agagactttt ttttaggaga gttggagaag tgccttgaag atccagaaaa
     6481 actcggatcc ctttttgtaa agcacgagag acggttgcac atgtacatag tttattgtca
     6541 aaataagcca aagtctgagc acattgtgtc cgagtacatc gacacctttt ttgaggactt
     6601 aaagcagcgc ctgggccaca gactgcagct cactgatctg ttgatcaaac cggtgcagag
     6661 aatcatgaag taccagctgc tcctgaagga cttcctcaag tactcaaaaa aagctagcct
     6721 ggacacatcc gaattagaga aagctgtaga agtcatgtgc atcgtgccga agcgctgtaa
     6781 tgacatgatg aacgtcgggc ggctgcaggg atttgacggt aaaattgttg cccagggtaa
     6841 actgctctta caggacacat tcctggtcac agatcaagac gcaggacttc tgcctcgatg
     6901 caaagagagg cgggttttcc tctttgaaca gatcgtcata ttcagcgaac cacttgataa
     6961 aaaaaagggc ttttccatgc caggatttct gttcaagaac agtatcaagg tgagttgcct
     7021 ctgcttggag gaaaatgtgg agagcgatcc ctgtaagttc gccctgacgt ccaggacggg
     7081 cgatgccgtg gagactttcg ttttgcattc atccagtcca agcgtccggc aaacgtggat
     7141 ccatgagatc aaccaaatct tagaaaacca gcgcaacttt ttaaacgcct tgacatcacc
     7201 catcgagtac cagaggaacc atagtggggg cggcggcagt ggcagcgggg gcagcagcgg
     7261 cgggggcggg ggcagcggcg gcagcggggc ctccagcgga ggcagcagca gccatggcag
     7321 tggccccagc agctgtagca gtggccccag ctcgagcagg agcaggccct ccaggatccc
     7381 acagcctgtc agacaccacc cccccatgct ggtctcctct gcagcctcaa gccaggcaga
     7441 ggcagacaag atgtcaggta tgtctgctcc cagcccctca ctgcctaccc ccagcagcag
     7501 cctggccctt gaggccagcc ttggccagcc cagcaggctc cccctgagtg gagactccga
     7561 aggtcatgag cgagaaacag aacccattcc caagatgaag gtgatggaga gcccccggaa
     7621 ggccccgggg agtacttccg gtacaagcca agacggaaac accaaggatg cccggggaaa
     7681 cctgggctca ctgcctctgg gcaagactag acctggggcc gtctcaccgc tgaactctcc
     7741 tctctccact acgttccctt ctccttttgg caaggaggcc tttcctccca gcagcccctt
     7801 gcagaagggg ggctccttct ggagctccat tcctgcttcc cctgccagcc ggccaagctc
     7861 cttcaccttc ccgggggaca gtgactccct ccagcgacag acgcaccgcc atgcagcccc
     7921 cagcaaggac acagaccgca tgagcacgtg ctcctcggcc agcgagcagt ccgtgcagtc
     7981 cactcagagc aacgggagtg aaggcagcag cagtagcaac atctccacca tgctggtgac
     8041 acacgagtac acggcagtga aggaggatga gataaacgtc tatcaaggag aggtcgttca
     8101 aattctggcc agcaaccagc agaacatgtt tctggtgttc cgagccgcca ctgaccagtg
     8161 ccccgcagcc gagggctgga ttccgggctt cgtcttgggc catacgagtg cagttatcat
     8221 ggagaacccc gacgggactc tcaagaaatc aacatcttgg cacacagcac tccgtttaag
     8281 gaagaagtct gagaagaaag ataaagacgg caaaagggat ggcaagttgg agaatgggta
     8341 caggaagcca cgagaagggc tcagtaacaa ggtatctgtg aagcttctta accccaacta
     8401 catttatgac gttcccccag aatttgttat cccattgagt gaggtcacgt gcgagacagg
     8461 ggagaccgtt gtttttagat gtcgtgtctg tggccgcccc aaggcctcaa tcacctggaa
     8521 gggccccgaa cacaacactt tgaacaacga tgaccactac agcatctcct acagtgacat
     8581 tggggaggcc actctgaaga tcattggtgt aagcacagaa gatgatggca tctacacctg
     8641 catcgctgtg aatgacatgg gttcagcctc atcttctgcc agcctgaggg ttctaggtcc
     8701 agggagtgat gggatcgtgg tgacttggaa ggacaacttc gacgccttct acagtgaagt
     8761 ggctgagctt ggcaggggca ggtttgctgt agttaagaaa tgtgaccaga agggaaccaa
     8821 acgagcagtg gccacaaagt ttgtgaacaa gaagttgatg aagcgtgacc aggtcactca
     8881 tgagctcggc atcctgcaga acctgcagca ccccctcctc gtgagccttc ttgacacctt
     8941 cgagaccccc accagttacg tcctggttct ggaaatggct gaccaaggtc gcctccttga
     9001 ctgtgtggtc cggtggggaa gcctcaccga agggaaggtc agagcgcacc tgggggaagt
     9061 tctggaagcc gtccgatacc ttcacaactg caggatagca cacctggacc taaagcctga
     9121 gaatatcttg gtggatcaga gtttggccaa gccaaccatt aaactggctg actttggaga
     9181 cgctgtccag ctcaacacca cctactacat ccaccagttg ctggggaacc ccgagtttgc
     9241 agccccggaa atcattcttg ggaaccctgt ctccctgacc gcggacacat ggagtgttgg
     9301 ggtgctcacc tacgtgctgc tcagcggcgt gtcccccttc ctggatgaca gcgtggaaga
     9361 gacctgcctg aatatttgcc ggctggactt tagcttccca gaggactact ttcaaggagt
     9421 tagccagaag gccaaagaat tcgtgtgctt cctcctacag gaggaccccg ccaagcgtcc
     9481 ctcggctgca ctggccctgc aggaacagtg gctgcaggca ggcaacggca gcggcaaagg
     9541 cacgggcgtc ctcgacacgt ccagactgac ctccttcatt gagcggcgca aacaccagaa
     9601 tgatgttcga cctatccgta gcattaaaaa cttcttacag agcaggcttc tgcctagagt
     9661 ttgacctatc ttgaagttct ttctcattct ctttcacctg ccaatcagct gttaatttga
     9721 atttttgaag aggaaaccaa accaaaacta agtggccggc cgccggctgt tcatcgtgtg
     9781 aaatggcatt ccaagtgagc tgtgctcaac agcgcttggg ctcagagctg caaggtgcgc
     9841 tggggtggag gaccttcagt tacactctgc atgggcagag accgcattgc tggatcacag
     9901 tattttattc aggttcctgc aaaaataata ataataataa taaaaaagag atactttttt
     9961 taatgaacat ggatagaatt ttgcaaattt aacattttca agatttattc aaaaaaaaaa
    10021 aaaaacacat gcctatgttc aaaccactgg tgttacacag aactgagaaa ctgtgcattt
    10081 ctgggagaga ctcgccaggt ggcagccact cccatgggct cagccagtgc ttggtgtcca
    10141 cctggcaccg agcttttcca gctgcctcgt ctgggtacat cctgccgggc accagacttg
    10201 gcttctgaga tgtgtgttca acctcaatct tttgcataaa atataatgaa tggttttgct
    10261 ctgatgaaat gtaggcctta cttgtacata agactcttcc tgccttttgt ctggcaatcc
    10321 cccacccctt cccctcctac ccaccgcctg ggccttcctc tcggggggtc tgatggggtc
    10381 tgtccatcca atggagactt gaggctgggt ccagccccac tgccccctcc ccagccccac
    10441 caccccaaac cgtcaatcag attgttgaac ggtatacagt cagatgaaaa tactgtaaat
    10501 gcactcattg gggttttgtt tgttttggtt tttttgtttg ttttggtttt tgttgttttt
    10561 atttcatacc atgtgcaatg ttgtggcttt aacattttct gcaactattt atgaagactt
    10621 ctgttataca tgtaataaat atatagaaaa agcacatact tcgcaagtga gctttatggt
    10681 tctgtgtgtg ttgggggtgg gtcgtagccc tgtgccatcc gtcaaggaca cttaactctt
    10741 cgtgaaattt gtcagttttg aggactgtaa aaagtgattt catactctga atataaaact
    10801 ggataatagg gtaatgtttt aaattttatt atgctattat tcagaatgcc aaagtattat
    10861 ttttttccca gaatcagtct gaacatttac tactttttag actttttgac attcaacttt
    10921 ctgtataaaa attggctgga ttttaagctt ttggtaagaa agccaattaa gtactggcca
    10981 ccctgaggct ggtacgcagt gcccgagtca ctgtggcgga gctggcgctg gggtgggaag
    11041 tccttcaaca caagaagttg gatttctggc aagcttggat ttttctggat gtctttttat
    11101 ctttattgtg tgaaatacac ttaaaaaaga gaccagcttg cttcccaagc cagacagaca
    11161 tctgggtcgt gagccataaa ctggcataat taagctttgc agtttccggt gtatttattt
    11221 attcttctgt tgggtttttt tgtttgtttg tttcttgtaa aattgtacag aaacttttta
    11281 agagaaattg gattcaaaaa ctggatgtgt atctgtaacc tcataacttt tatttgtgta
    11341 ctgttttctt tgattatttc agttaaaact tttacattat ttttttgcat gtatatttct
    11401 ttgtacagag accgtacatg tttacacagt atgatgtgat gtaaatattt tattttgcca
    11461 tcagttattt taaaaaatta aacatatttg cctgataaaa aaaaaaaaaa aaaaaaaaaa
    11521 a
//
