LOCUS       NM_080510               1745 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus tripartite motif-containing 69 (Trim69), transcript
            variant 1, mRNA.
ACCESSION   NM_080510
VERSION     NM_080510.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   He,X., Xie,W., Li,H., Cui,Y., Wang,Y., Guo,X. and Sha,J.
  TITLE     The testis-specifically expressed gene Trim69 is not essential for
            fertility in mice
  JOURNAL   J Biomed Res 35 (1), 47-60 (2020)
   PUBMED   33273151
REFERENCE   3  (bases 1 to 1745)
  AUTHORS   Li,L.J., Zheng,J.C., Kang,R. and Yan,J.Q.
  TITLE     Targeting Trim69 alleviates high fat diet (HFD)-induced hippocampal
            injury in mice by inhibiting apoptosis and inflammation through
            ASK1 inactivation
  JOURNAL   Biochem Biophys Res Commun 515 (4), 658-664 (2019)
   PUBMED   31178136
  REMARK    GeneRIF: Trim69 might be a functionally essential inhibitor of ASK1
            activation during the pathogenesis of hippocampal inflammation and
            apoptosis
REFERENCE   4  (bases 1 to 1745)
  AUTHORS   Shyu,H.W., Hsu,S.H., Hsieh-Li,H.M. and Li,H.
  TITLE     Forced expression of RNF36 induces cell apoptosis
  JOURNAL   Exp Cell Res 287 (2), 301-313 (2003)
   PUBMED   12837286
REFERENCE   5  (bases 1 to 1745)
  AUTHORS   Shyu,H.W., Hsu,S.H., Hsieh-Li,H.M. and Li,H.
  TITLE     A novel member of the RBCC family, Trif, expressed specifically in
            the spermatids of mouse testis
  JOURNAL   Mech Dev 108 (1-2), 213-216 (2001)
   PUBMED   11578878
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845457.16 and AL844573.4.
            
            On Mar 31, 2020 this sequence version replaced NM_080510.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC050815.1, AK014923.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-189               AL845457.16        206434-206622
            190-669             AL845457.16        213281-213760
            670-765             AL845457.16        215728-215823
            766-999             AL845457.16        218880-219113
            1000-1022           AL845457.16        219966-219988
            1023-1147           AL845457.16        220076-220200
            1148-1745           AL844573.4         5142-5739
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.55 cM"
     gene            1..1745
                     /gene="Trim69"
                     /gene_synonym="4921519C19Rik; Rnf36; Trif; Trimless"
                     /note="tripartite motif-containing 69"
                     /db_xref="GeneID:70928"
                     /db_xref="MGI:MGI:1918178"
     exon            1..189
                     /gene="Trim69"
                     /gene_synonym="4921519C19Rik; Rnf36; Trif; Trimless"
                     /inference="alignment:Splign:2.1.0"
     CDS             184..1686
                     /gene="Trim69"
                     /gene_synonym="4921519C19Rik; Rnf36; Trif; Trimless"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1; ring
                     finger protein 36; tripartite motif-containing protein 69;
                     RFP-like (B30.2) domain protein; testis-specific RING
                     finger protein; RING finger B-box coiled-coil
                     transcription factor; E3 ubiquitin-protein ligase TRIM69;
                     RING-type E3 ubiquitin transferase TRIM69"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase TRIM69 isoform 1"
                     /protein_id="NP_536771.2"
                     /db_xref="CCDS:CCDS16655.1"
                     /db_xref="GeneID:70928"
                     /db_xref="MGI:MGI:1918178"
                     /translation="MEVSSRPPSNFDPGNYVEMSDPTTTHLPSKVVIQDLTTELHCPL
                     CNDWFRDPLMLTCGHNFCQDCIQSFWKVHSKETFCPDCKMLCQYSNCTFNLVLEKLVE
                     KIKKLPLLKGHPQCPEHGENLKLFSKPEGKMICFQCKDARLSMGQSKDFLQISEAVRF
                     FTEELAIYQSQLQTTLKELQSLRTIQKDAISAYKDNKIQLQQNLSLEFLKLHQFLHNK
                     EKDILNDLRDEGKLLNEEMEVNLNQIQEQCLVAKDMLATIQARMEQQNSFDFLTDITK
                     LIESMEKGMKTIVPRQLIAKKLSLGRFKGPIQYIIWKEMQAILSPGPSQLTLDPKTAH
                     PNLVLSKSQTSVCHCDVKQVMPDDPERFDSSVAVLGSKGFTSGKWYWEIEVGKKTKWT
                     IGVVRESIIRKGSCPLTPEQGFWLLRLRNQTDLKALDLPSRSLTLGDLRRVGVYLDYE
                     GGQVSFYNATTMTHLYTFSSVFQEKLFPYLCPCLNDGGENKEPLHIVHPQ"
     exon            190..669
                     /gene="Trim69"
                     /gene_synonym="4921519C19Rik; Rnf36; Trif; Trimless"
                     /inference="alignment:Splign:2.1.0"
     exon            670..765
                     /gene="Trim69"
                     /gene_synonym="4921519C19Rik; Rnf36; Trif; Trimless"
                     /inference="alignment:Splign:2.1.0"
     exon            766..999
                     /gene="Trim69"
                     /gene_synonym="4921519C19Rik; Rnf36; Trif; Trimless"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1022
                     /gene="Trim69"
                     /gene_synonym="4921519C19Rik; Rnf36; Trif; Trimless"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1147
                     /gene="Trim69"
                     /gene_synonym="4921519C19Rik; Rnf36; Trif; Trimless"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1745
                     /gene="Trim69"
                     /gene_synonym="4921519C19Rik; Rnf36; Trif; Trimless"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1720..1725
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trim69"
                     /gene_synonym="4921519C19Rik; Rnf36; Trif; Trimless"
                     /note="hexamer: ATTAAA"
     polyA_site      1745
                     /gene="Trim69"
                     /gene_synonym="4921519C19Rik; Rnf36; Trif; Trimless"
                     /note="major polyA site"
ORIGIN      
        1 agctttggcc ttgtctgtct tcttcagctc ctgaactctc ttctccctcc tcctctcatg
       61 ggctctgagt ccattcctag catactaaca ggcccagaac tcttggtctg cagccatcct
      121 gggctcactg agcaaagcca agttgcctga ctctgccccc tggtggccta atgaccaagt
      181 ttcatggagg tatcttctcg gcccccctcc aactttgatc caggcaacta tgttgaaatg
      241 agtgatccaa ccaccaccca tttaccctca aaagtggtga tacaagatct taccacggag
      301 ctacactgcc ctctgtgtaa cgactggttc cgtgatccgc tgatgctgac ctgtggccac
      361 aacttctgcc aagactgtat tcaaagcttc tggaaagtgc attcaaagga aacattctgt
      421 cctgactgta agatgctatg ccagtatagc aactgtacat tcaacctcgt gctggagaag
      481 ctcgtggaga agattaagaa gttacccctg ctcaagggcc atccacagtg cccagagcat
      541 ggagagaacc tgaaactgtt cagtaagcca gaaggcaaaa tgatctgctt tcaatgcaag
      601 gatgcacggt tgtctatggg gcagtctaag gatttcctgc agatctctga agctgtccgt
      661 ttctttacgg aggagcttgc tatctatcag agccaacttc agacaaccct gaaggagctt
      721 cagtctctca gaacaattca gaaagatgct atttctgctt acaaggacaa caagatacaa
      781 ctgcaacaaa acctttcctt ggagtttcta aagctacatc agtttctgca caacaaagaa
      841 aaggacattc taaatgacct ccgggatgag gggaaactct tgaatgagga gatggaggtg
      901 aatctgaacc agattcagga gcagtgtctt gttgccaaag acatgctggc gaccattcag
      961 gcacggatgg aacagcagaa ctccttcgac ttcctcacag acatcacaaa gctcatagag
     1021 agcatggaga aaggaatgaa gacaattgta cctagacagc tcatcgccaa aaagctgagc
     1081 ctaggccgct tcaaaggtcc tatccagtat atcatctgga aggaaatgca ggctattctc
     1141 tctccaggcc catctcaatt aactctggat cctaaaacag ctcatcccaa tctggtgtta
     1201 tccaaaagcc agaccagtgt gtgtcactgt gatgttaagc aggtaatgcc tgatgatcca
     1261 gagcggtttg attcaagtgt ggctgtactg ggctcaaaag gcttcacctc tggaaaatgg
     1321 tactgggaaa tagaagtagg aaagaagaca aaatggacaa ttggagtcgt cagagagtcc
     1381 atcattcgga aggggagctg ccctctgact ccagagcaag gattctggct tctaagacta
     1441 agaaaccaaa cagatctaaa ggccctggat ttgccctccc gtagtctgac tctaggcgac
     1501 ctcagaagag tgggcgtgta cctggattat gagggcgggc aagtatcctt ctacaacgct
     1561 acaaccatga cccatcttta cacctttagc agtgttttcc aggagaaact tttcccctac
     1621 ttgtgcccct gccttaatga cggtggggag aataaagaac cgttgcacat cgtacatcca
     1681 cagtaattaa gttatactat tgtataaatt cagggcttta ttaaagtatt gaaatagttt
     1741 accaa
//
