LOCUS       NM_178802               3050 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus tripartite motif-containing 65 (Trim65), mRNA.
ACCESSION   NM_178802
VERSION     NM_178802.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Jiang,M., Wang,D., Su,N., Lou,W., Chen,Y., Yang,H., Chen,C., Xi,F.,
            Chen,Y., Deng,L. and Tang,X.
  TITLE     TRIM65 knockout inhibits the development of HCC by polarization
            tumor-associated macrophages towards M1 phenotype via JAK1/STAT1
            signaling pathway
  JOURNAL   Int Immunopharmacol 128, 111494 (2024)
   PUBMED   38218012
  REMARK    GeneRIF: TRIM65 knockout inhibits the development of HCC by
            polarization tumor-associated macrophages towards M1 phenotype via
            JAK1/STAT1 signaling pathway.
REFERENCE   3  (bases 1 to 3050)
  AUTHORS   Huang,Y., Chen,T., Jiang,M., Xiong,C., Mei,C., Nie,J., Zhang,Q.,
            Zhu,Q., Huang,X., Zhang,X. and Li,Y.
  TITLE     E3 ligase TRIM65 alleviates intestinal ischemia/reperfusion injury
            through inhibition of TOX4-mediated apoptosis
  JOURNAL   Cell Death Dis 15 (1), 29 (2024)
   PUBMED   38212319
  REMARK    GeneRIF: E3 ligase TRIM65 alleviates intestinal
            ischemia/reperfusion injury through inhibition of TOX4-mediated
            apoptosis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3050)
  AUTHORS   Ma,X.F., Zhou,Y.R., Zhou,Z.X., Liu,H.T., Zhou,B.B., Deng,N.H.,
            Zhou,K., Tian,Z., Wu,Z.F., Liu,X.Y., Fu,M.G. and Jiang,Z.S.
  TITLE     TRIM65 Suppresses oxLDL-induced Endothelial Inflammation by
            Interaction with VCAM-1 in Atherogenesis
  JOURNAL   Curr Med Chem 31 (30), 4898-4911 (2024)
   PUBMED   37608612
  REMARK    GeneRIF: TRIM65 Suppresses oxLDL-induced Endothelial Inflammation
            by Interaction with VCAM-1 in Atherogenesis.
REFERENCE   5  (bases 1 to 3050)
  AUTHORS   Liu,H., Zhou,Z., Deng,H., Tian,Z., Wu,Z., Liu,X., Ren,Z. and
            Jiang,Z.
  TITLE     Trim65 attenuates isoproterenol-induced cardiac hypertrophy by
            promoting autophagy and ameliorating mitochondrial dysfunction via
            the Jak1/Stat1 signaling pathway
  JOURNAL   Eur J Pharmacol 949, 175735 (2023)
   PUBMED   37080331
  REMARK    GeneRIF: Trim65 attenuates isoproterenol-induced cardiac
            hypertrophy by promoting autophagy and ameliorating mitochondrial
            dysfunction via the Jak1/Stat1 signaling pathway.
REFERENCE   6  (bases 1 to 3050)
  AUTHORS   Tang,T., Li,P., Zhou,X., Wang,R., Fan,X., Yang,M. and Qi,K.
  TITLE     The E3 Ubiquitin Ligase TRIM65 Negatively Regulates Inflammasome
            Activation Through Promoting Ubiquitination of NLRP3
  JOURNAL   Front Immunol 12, 741839 (2021)
   PUBMED   34512673
  REMARK    GeneRIF: The E3 Ubiquitin Ligase TRIM65 Negatively Regulates
            Inflammasome Activation Through Promoting Ubiquitination of NLRP3.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3050)
  AUTHORS   Li,Y., Huang,X., Guo,F., Lei,T., Li,S., Monaghan-Nichols,P.,
            Jiang,Z., Xin,H.B. and Fu,M.
  TITLE     TRIM65 E3 ligase targets VCAM-1 degradation to limit LPS-induced
            lung inflammation
  JOURNAL   J Mol Cell Biol 12 (3), 190-201 (2020)
   PUBMED   31310649
REFERENCE   8  (bases 1 to 3050)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 3050)
  AUTHORS   Lang,X., Tang,T., Jin,T., Ding,C., Zhou,R. and Jiang,W.
  TITLE     TRIM65-catalized ubiquitination is essential for MDA5-mediated
            antiviral innate immunity
  JOURNAL   J Exp Med 214 (2), 459-473 (2017)
   PUBMED   28031478
  REMARK    GeneRIF: TRIM65 as an essential component for the MDA5 signaling
            pathway and provide physiological evidence showing that
            ubiquitination is important for MDA5 oligomerization and
            activation.
REFERENCE   10 (bases 1 to 3050)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK079982.1, AK028857.1, AK050080.1 and BE850668.1.
            
            On Jan 9, 2008 this sequence version replaced NM_178802.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK047439.1, AK028857.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN01164137
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-112               AK079982.1         1-112
            113-2426            AK028857.1         2-2315
            2427-2427           AK050080.1         2340-2340
            2428-2549           AK028857.1         2316-2437
            2550-2656           AK050080.1         2465-2571
            2657-2969           AK028857.1         2544-2856
            2970-3050           BE850668.1         10-90               c
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 80.91 cM"
     gene            1..3050
                     /gene="Trim65"
                     /gene_synonym="4732463G12Rik"
                     /note="tripartite motif-containing 65"
                     /db_xref="GeneID:338364"
                     /db_xref="MGI:MGI:2442815"
     exon            1..543
                     /gene="Trim65"
                     /gene_synonym="4732463G12Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..1692
                     /gene="Trim65"
                     /gene_synonym="4732463G12Rik"
                     /EC_number="2.3.2.27"
                     /note="tripartite motif-containing protein 65"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase TRIM65"
                     /protein_id="NP_848917.2"
                     /db_xref="CCDS:CCDS48981.1"
                     /db_xref="GeneID:338364"
                     /db_xref="MGI:MGI:2442815"
                     /translation="MAAQLLEEDVVTCSICLGRYRDPVTLPCGHSFCGNCIQDSWRSC
                     EKSCPECRQPFPEGAKLSRNVKMSTLLQALPVLPAPPAVTPRRDSATSHSARCLRHGR
                     PLEFFCRTEGLCVCSACTVHDCSHHERALLDVERRVREDQLRARVLVTQQQVAQAETQ
                     LQELQEQRSRIESSACTLASVVSRRFSSLLQALEKQQASTLSDIEVAKKQALGQVLNE
                     KQRLTDHLRALSQYDQSVQDLLAQADDCIFFQELQQLPEPTESLGPLTSPQWNEEQQL
                     SNVNQLLSPLCELLLEEKSLPKVAAKAADAGPLETLGPLAPVPSAVCPLRKKLWQNYR
                     NLTFDPETANQYLHLSHKDQRVTHHFQAQGPAKSGSFELWQVQCTQSFQTGQHYWEVR
                     VSDHSVTLGVTYPKLSRQKLGTHTDNIGRGPCSWGLCIQEDSMQAWHNGKSQRLRGLP
                     GQLLGVDLNLTSGCLTFYSLEPRTQPLHTFYAVFSQPLFPVFWLLEGRTLTLCHQPEA
                     TLPARPQEEATAPS"
     exon            544..639
                     /gene="Trim65"
                     /gene_synonym="4732463G12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            640..873
                     /gene="Trim65"
                     /gene_synonym="4732463G12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            874..1051
                     /gene="Trim65"
                     /gene_synonym="4732463G12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1117
                     /gene="Trim65"
                     /gene_synonym="4732463G12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..3050
                     /gene="Trim65"
                     /gene_synonym="4732463G12Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2951..2956
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trim65"
                     /gene_synonym="4732463G12Rik"
                     /note="hexamer: CATAAA"
     polyA_site      2970
                     /gene="Trim65"
                     /gene_synonym="4732463G12Rik"
                     /note="major polyA site"
ORIGIN      
        1 gaacgttcta gtgcaccagg ctgcgcactg ccgcgacttc ctggactgtc ttccccaacg
       61 tagtgctcgc actggtagcc tcggcccctt taagagatct gagcactggg cctcgcttcc
      121 gccatggccg ctcagttgct ggaggaggac gtggtgactt gctccatctg cttaggtcgc
      181 taccgggacc cggtgacgct gccctgcggg cacagcttct gcgggaactg catccaggat
      241 tcatggcgtt cctgcgaaaa gtcgtgtccg gaatgtcgcc agcccttccc cgagggcgcc
      301 aagctgtccc gcaacgtgaa gatgagcacc ttgctgcagg cgctgcccgt tctgccggcg
      361 ccgcccgccg tgactccgcg ccgggactcc gccacaagcc acagcgctcg ctgcctgcgc
      421 cacgggaggc cgctcgagtt cttctgtcgc acggagggcc tctgcgtgtg cagcgcgtgc
      481 acggtgcacg actgcagcca ccacgagcgg gcgctgctgg acgtggagcg ccgcgtgcgc
      541 gaggaccagc tgagagcgag ggtactggtt acccagcagc aggttgccca ggcagagacc
      601 cagcttcagg agctgcagga gcaacgcagt cggattgaga gttcagcctg caccctggcc
      661 tcggtggtct ccaggagatt cagtagcctg cttcaggcgc tggagaagca gcaggcctca
      721 acactgagtg atatagaggt agccaagaag caggcgctgg gccaagttct gaatgagaaa
      781 cagcggctga ctgaccacct aagggccctg tctcagtatg atcagagtgt ccaggatctc
      841 ctggcacagg cggatgactg catcttcttc caggaattac agcagctccc tgagcccaca
      901 gagtccctcg ggccactgac ctctccacag tggaatgaag aacagcagct gagcaacgtg
      961 aaccagctgc ttagccccct gtgtgagctc ctccttgaag agaagagcct ccccaaggtg
     1021 gcagccaagg ctgcagatgc tggccctttg gagaccctgg gtcccctggc accagtcccc
     1081 agcgcagtgt gtccactgag gaagaagctc tggcagaatt atcgcaatct gacctttgac
     1141 ccagagaccg ccaaccagta tttgcacttg tctcacaagg accagcgggt gacacaccat
     1201 ttccaggccc agggcccagc caagtcgggc agcttcgagc tgtggcaggt gcagtgtacc
     1261 cagagttttc agactggaca acattactgg gaggtacgtg tctctgatca ctctgtcact
     1321 ctgggcgtca cctacccaaa gctatcacgc caaaagctgg ggacccacac ggataacatc
     1381 ggccgtggac cctgctcctg gggcctctgc atccaggagg acagtatgca ggcctggcac
     1441 aatggcaaat ctcagcgcct gcgtggattg cccggacaac tcctgggcgt agatttgaac
     1501 ttgacctctg gctgcctcac cttctacagc ctggagccac gcacacagcc gctgcacact
     1561 ttctatgcag tgttcagcca gcctctcttc cctgtcttct ggctccttga ggggaggacc
     1621 cttactctgt gccatcagcc tgaggccacg ctccctgcaa ggccccagga agaggccaca
     1681 gcacccagct gaggaaggca cagacaggtg ccaccgtgcc tgggtgccca aaagtggccc
     1741 cacctctagc ttctagaagg aacagctgtt ggcctctctg ttgactcaga gacggacatg
     1801 ggagaggagc cttagaacaa agtttacttt ttactatcta tgaaggggac aggggacaga
     1861 gagaaattaa ataggaagtc ccagccccaa gcaagaacca ggttcttagc attgcgtgga
     1921 catctatctt agtcactgtt ctgttgctgt gaagagacac catgactaag gcaactctat
     1981 agaagaaagc gtttaactca gggcttgcta acagttttag cccatgatta tcatggaggg
     2041 aagcagacag gcgtggtgct agagcagtag tcaggagctt cacatcctga tcaccaggcg
     2101 catgggcacg agtgcgcacg cacacacaca cacacacaca cacacacact cacgcacaca
     2161 gactagcata ggcttttgta tatttaaagt gacacactcc cccaacaagg ccacacctcc
     2221 taatccctct caaacagttc caccaagtgg ggactaagcg cacaggctgt agacagccaa
     2281 tgtggtgatg agtatgggtg cagcagatgt ggtgtggcag atgtgaaccg ctttcctttg
     2341 cttgtcccac atatgcttcc atgaaggttc tgggtggttt tttgctcaca tttctggaag
     2401 tagaattttt tttttttttt tttttttgta ctgggtatag agtctgaaat gttttgaatg
     2461 ctaacattca gagttctgat ctctcactga caacatctcc agcctggggt gatttattta
     2521 cttgagagcc ccccacctcc cccccccccc atgttctgat gttggtggta gttacaagga
     2581 agcccacttt tgtaatgtcc ttttcttttt gtaattcctt aagcatttgt tttgtatgct
     2641 cgtgggtaaa catctcacaa aaaaattaga aattctttca tgtttattta gtgtgggact
     2701 cagagtgggg tggcttgtgt acgccatggc ttacgtgtgg aggtcagagg acaacttgca
     2761 ggagtccgat ctctccttct accatgtggg ttctggagtg gaacccagac tgtcacgctt
     2821 gacagcaagc acattgggat agctgcagtt atcccatcat cccgacaata acaggttttt
     2881 gttgtttgaa acacgatatc tctatgaacc cctggttggc tcagaactat gtagaccagg
     2941 ctgtccaact cataaaaatc tgaaccccca tcagtggcct ctaagagttt ttaaagaatt
     3001 atatgtgcaa taatatattc cctagggaag atataaatat tttatatgaa
//
