LOCUS       NM_194343               4615 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus tripartite motif-containing 45 (Trim45), transcript
            variant 2, mRNA.
ACCESSION   NM_194343 XM_131105 XM_207779
VERSION     NM_194343.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Huang,X., Ye,C., Zhao,X., Tong,Y., Lin,W., Huang,Q., Zheng,Y.,
            Wang,J., Zhang,A. and Mo,Y.
  TITLE     TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in
            septic encephalopathy
  JOURNAL   J Neuroinflammation 20 (1), 284 (2023)
   PUBMED   38037161
  REMARK    GeneRIF: TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis
            in septic encephalopathy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4615)
  AUTHORS   Zhang,J., Cao,L., Gao,A., Ren,R., Yu,L., Li,Q., Liu,Y., Qi,W.,
            Hou,Y., Sui,W., Su,G., Zhang,Y., Zhang,C. and Zhang,M.
  TITLE     E3 ligase RNF99 negatively regulates TLR-mediated inflammatory
            immune response via K48-linked ubiquitination of TAB2
  JOURNAL   Cell Death Differ 30 (4), 966-978 (2023)
   PUBMED   36681779
REFERENCE   4  (bases 1 to 4615)
  AUTHORS   Xia,Q., Zhan,G., Mao,M., Zhao,Y. and Li,X.
  TITLE     TRIM45 causes neuronal damage by aggravating microglia-mediated
            neuroinflammation upon cerebral ischemia and reperfusion injury
  JOURNAL   Exp Mol Med 54 (2), 180-193 (2022)
   PUBMED   35217833
  REMARK    GeneRIF: TRIM45 causes neuronal damage by aggravating
            microglia-mediated neuroinflammation upon cerebral ischemia and
            reperfusion injury.
REFERENCE   5  (bases 1 to 4615)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4615)
  AUTHORS   Perez-Garcia,V., Fineberg,E., Wilson,R., Murray,A., Mazzeo,C.I.,
            Tudor,C., Sienerth,A., White,J.K., Tuck,E., Ryder,E.J., Gleeson,D.,
            Siragher,E., Wardle-Jones,H., Staudt,N., Wali,N., Collins,J.,
            Geyer,S., Busch-Nentwich,E.M., Galli,A., Smith,J.C., Robertson,E.,
            Adams,D.J., Weninger,W.J., Mohun,T. and Hemberger,M.
  TITLE     Placentation defects are highly prevalent in embryonic lethal mouse
            mutants
  JOURNAL   Nature 555 (7697), 463-468 (2018)
   PUBMED   29539633
REFERENCE   7  (bases 1 to 4615)
  AUTHORS   Bassett,J.H., Gogakos,A., White,J.K., Evans,H., Jacques,R.M., van
            der Spek,A.H., Ramirez-Solis,R., Ryder,E., Sunter,D., Boyde,A.,
            Campbell,M.J., Croucher,P.I. and Williams,G.R.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Rapid-throughput skeletal phenotyping of 100 knockout mice
            identifies 9 new genes that determine bone strength
  JOURNAL   PLoS Genet 8 (8), e1002858 (2012)
   PUBMED   22876197
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK150574.1 and BC057358.1.
            
            On Sep 30, 2009 this sequence version replaced NM_194343.1.
            
            Transcript Variant: This variant (2) lacks an alternate exon that
            results in a frameshift in the 3' coding region, compared to
            variant 1. The encoded isoform (b) has a distinct C-terminus and is
            shorter than isoform a. Both variants 2 and 3 encode the same
            isoform.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057358.1, SRR1660811.244715.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1168              AK150574.1         2-1169
            1169-4615           BC057358.1         1047-4493
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 43.85 cM"
     gene            1..4615
                     /gene="Trim45"
                     /gene_synonym="4921530N01Rik"
                     /note="tripartite motif-containing 45"
                     /db_xref="GeneID:229644"
                     /db_xref="MGI:MGI:1918187"
     exon            1..907
                     /gene="Trim45"
                     /gene_synonym="4921530N01Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             420..2162
                     /gene="Trim45"
                     /gene_synonym="4921530N01Rik"
                     /EC_number="2.3.2.27"
                     /note="isoform b is encoded by transcript variant 2;
                     tripartite motif-containing protein 45; RING finger
                     protein 99; E3 ubiquitin-protein ligase TRIM45"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase TRIM45 isoform b"
                     /protein_id="NP_919324.2"
                     /db_xref="CCDS:CCDS17678.1"
                     /db_xref="GeneID:229644"
                     /db_xref="MGI:MGI:1918187"
                     /translation="MSEIRKPLLGFVHKLQDANASGSSGKTHCPTCLRLFKVPRLLPC
                     LHTVCTTCLEKLDPFSVVDIRGGDSDTSSEGSVFQDPELCSLQPQIGILCPVCDAQVD
                     LPLGGVKALTVDHLAMNDVLLENLRGEGQGLVCDLCSDREVEKRCQTCKANLCHFCCQ
                     AHRRQKKTTYHTMVDLKDLKGYSQVGKPILCPSHPAEELRLFCELCDRPVCRDCVVGE
                     HREHPYDFTSNVIHKHGDSVRELLRDTQPHVEALEDALAQIKSVNNALQERVEAVAAD
                     VRTFSEGYIKAIEEHRDKLLQQLDDIRIQRETALQLQKAQLEQLLADMRTGVEFTEHL
                     LTSGSDLEILITKGVVVERLRKLNKVEYSARPGVNHKICFSPQEKAGQCQGYEVYGAI
                     NTQEVDPAQCVLQGEDLHRAREKQTASFTLFCKDASGQSMGRGGDNVHVEVVPKDKKD
                     SPIRTVVQDNKDGSYRVSYTPKEPGIYTVWVCIREQHVQGSPFNVTVRRKHRPHPGVF
                     HCCTFCSSGGQKAARCACGGTMPGGYLGCGHGHKGHPGRPHWSCCGKFIEKSECSFTS
                     GQGAPRSLLRTVAL"
     exon            908..1641
                     /gene="Trim45"
                     /gene_synonym="4921530N01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1771
                     /gene="Trim45"
                     /gene_synonym="4921530N01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1772..1886
                     /gene="Trim45"
                     /gene_synonym="4921530N01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1887..2013
                     /gene="Trim45"
                     /gene_synonym="4921530N01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2014..4594
                     /gene="Trim45"
                     /gene_synonym="4921530N01Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2263..2268
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trim45"
                     /gene_synonym="4921530N01Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2287
                     /gene="Trim45"
                     /gene_synonym="4921530N01Rik"
                     /note="major polyA site"
     regulatory      4575..4580
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trim45"
                     /gene_synonym="4921530N01Rik"
                     /note="hexamer: AATAAA"
     polyA_site      4594
                     /gene="Trim45"
                     /gene_synonym="4921530N01Rik"
ORIGIN      
        1 actctgggag agcccgagga cccactacct catcgcccgg agggcgcgct tgtggcgtgg
       61 tgggggcggg agggagggtg accttggagg acaagaaatg ctggggtcag ggtggtggtg
      121 gcagaattag ctggggacta gctctagaag cgcttgcgag gaggacgatg tgggtgatcc
      181 aggagggtgt ggctgtgact ggtgtgggat gcagtggaaa ggaagggcca aaccacaaat
      241 ccggagatct gtggatggtt cctcagaggc gttctctttc tggttgtcct tttacttggc
      301 aacacgagag tcctcatggt cagtgttcaa gatcttggaa ctctgtcttt gagtaaatct
      361 aacctgcatt agaggactgc gcagggcctt ttgtgatact gggtacatac gtggggaaga
      421 tgtcagaaat caggaagccg cttctgggtt tcgtgcacaa actccaagat gcgaacgcgt
      481 ctggaagctc aggcaagact cattgtccta catgtctgag gcttttcaaa gtccccaggc
      541 tcttgccttg cttgcacaca gtgtgcacca cgtgtctgga aaagctggat ccattctcag
      601 tagtggacat ccgtgggggt gactcagata caagctctga ggggtcagta ttccaggacc
      661 ccgagttatg tagcctgcag ccacagatcg gcatcctctg tccggtatgt gatgctcaag
      721 tggacctgcc cttgggtgga gtgaaggctt taacggtaga ccacctggcc atgaatgatg
      781 tgctgctgga gaacctgcgt ggggaaggcc agggcctggt gtgtgacctg tgcagcgaca
      841 gagaagtaga gaagaggtgt cagacctgca aagccaatct ctgccacttc tgctgccagg
      901 ctcataggcg acagaagaag acgacatatc acaccatggt ggacctaaaa gacctgaaag
      961 gctacagcca ggttggaaag cccatcctat gtccttctca ccctgcagag gagctgaggc
     1021 tgttctgtga actctgtgac cggccggtgt gtcgggactg tgtagttggg gagcaccgag
     1081 agcaccccta tgacttcacc agcaatgtca tccacaagca tggagattct gtgcgggagc
     1141 tgcttcgaga cacccagccc catgtggaag ccctggaaga tgccctggca cagattaaaa
     1201 gtgtgaacaa cgccctccaa gagcgagtgg aggcagtggc agctgacgtc cggacattct
     1261 ctgagggcta catcaaagcc atcgaggaac accgggacaa gctgctgcag cagctggatg
     1321 acatacggat ccagagggaa accgctttac agctgcagaa agcgcagctg gaacagctgc
     1381 tggcagatat gcggactgga gtggagttca ccgagcatct gctgaccagc ggctcggact
     1441 tagagattct catcaccaag ggagtggttg tagagcggct caggaagctg aataaagttg
     1501 aatacagtgc ccgcccagga gtgaaccata agatctgctt ctccccccag gagaaggcag
     1561 gccagtgcca aggctatgaa gtgtatgggg ccattaacac gcaggaagtt gatccagctc
     1621 agtgtgtcct tcagggagaa gatctccaca gagctcgaga gaaacagaca gcctctttta
     1681 ccttgttctg taaggatgcc tcgggacaga gcatgggcag gggaggagat aatgtccatg
     1741 tggaagttgt ccccaaagat aagaaggaca gtccgatcag aacagtggtc caggacaaca
     1801 aggatgggtc gtaccgcgtt tcctacaccc ccaaggagcc tggcatctac accgtgtggg
     1861 tctgcatcag agagcagcac gtgcagggct caccattcaa cgtgaccgtg aggaggaagc
     1921 atcggccaca tccgggtgtg tttcactgct gcaccttctg ctccagtgga ggacagaaag
     1981 ctgcccgctg tgcctgcgga ggcaccatgc cgggtggata ccttggctgt ggccacggac
     2041 acaaaggcca cccgggccgt ccacactggt cttgctgtgg gaagttcatt gagaagtccg
     2101 agtgctcgtt cacgagtggg cagggcgccc cgaggagtct gcttaggacc gtggcgctct
     2161 gatgcttcct ggggacaagt cgggccaggt aaccctgaag ctagcataac ttcagctatg
     2221 cagagaaccc agagcttgag tagttccaaa gagactgaca gaattaaaga cgaagtgctt
     2281 tctctaacat cagacttcaa gtgctttctg tgtgggcatc tctcgtgtac catggcggag
     2341 tgcaggcctc ccctacaccg ctcctcaagg caggctgctg atctactcca cggaaagctc
     2401 tcctgtttta tacgggcatt gaggtgtctg tgccatggga ccatgagctc ctggagcagg
     2461 gaggacttta tgccttcctt tccactgtgt tgcagcctct ccggtttacc ccgagctaga
     2521 gtttcagggc ttctcctgga ggatgaaacc cctctggccc atcatttgtt tgttgtatac
     2581 tttcagtggt ttcctagtac acagtttatg atgattatca cggttttgcc gtctagatga
     2641 gcgcccgagt gatgctcatg tgtaggcaca caagtagaca gtaaggaggt acggtgtggc
     2701 aagagcgcag ccatctgctg cagttcttta catcagatga cggcgggtcc tccatccaca
     2761 cagctctgac ttaatggagg gctttcgctg ccctctctac cctgcaacag ctgaaagcac
     2821 tgactctgca gggcaccccc tagcggctac aggattctgg gaggaagact tagtactccc
     2881 tcagcccagc caaggtcttc ccagtagaga ttttgtgttt tctgggattc ttggtttctt
     2941 acacagcagt aaactcacaa catatgaaac tgaattactg aagtttgcat tataggatct
     3001 catagggagt ggtgactcat tggaggggca attgggtaaa ggaaaggaag cgaggcactt
     3061 agatgaccac tgtcaggttt cacttccttc tcttagcctt taagataaga tacattaaga
     3121 taattaagac attaagatac tttcttcatt ttcttcgaac attccccttg agcccctttc
     3181 tggaagcaaa atctgatgtt cccagaagac aacactgccc ctctccccct cagctggttg
     3241 ttgtttgctc ctcactctgt ctgttcccac ccactcccag ccacaccctg agccaggtca
     3301 ggtgtctact gagcttgcca tggctgcttt gagatcactg gttctcagcc aggcaacccc
     3361 acctcccggg ggcgtgtctt acagaatctt ggggatatgg gttctagcag cattgagtag
     3421 ggccacaggt cagtttattc aactgcagga tagctttctg cagcaaatgg agcatgcttt
     3481 gcctccctgc agtgccattt tcatgtctga tgaggcgtac atcctgcatt ctgctcaatg
     3541 gttccctccc aggctcctaa gctcgacctt gcactgtgtt gttgtcagta tgctcagtag
     3601 agtcaaagag cagtccttcc atttctcgcc cctgtttcct cccgtgacct ccttctgact
     3661 taaataccac ttagactcgg tgactcccaa gtcctcttca ggtcactctc tagatgatta
     3721 tgaggtccag tgacttacgt catcacatct cctcttatgt gtacagtaag cccctcattc
     3781 aaatttaaat ggctcccagt gtccagttct cacttgctcc tgcctgtcat caccaaacag
     3841 cttctcctgg agtcctctcc acactttaaa agagaagcgt ctgtccttag gtggggcagc
     3901 ctggaaactc cttgtgtgtc acaccatatc caaaccactg gtaactcctg tcaacctcac
     3961 tttcactgac tcctgctctc tgcgtccatt gctaccatcc tggtctgaca agccgagact
     4021 cccaccagaa agctttgcct atcttctcgc cagcctgctt ccacatcagg tcattctgct
     4081 cggtgcctcc agtgaccgct cgctcatctc agaacagagg cagctcacct gcttcacatt
     4141 accatgtgcc agcgtggtct gatccatggc cttctcttcc tacccagctc caccttctgt
     4201 ccaggcacag tggccctgcc ggccgctgct caggctcagc agcctcttct tggagccttt
     4261 atagtaatgg gccttctatc tgacagtcct tctcaaagat gcatcatacc ctttgacttc
     4321 aagtttctgc tctagtgtca actattgtcc tattagtgag gtttctaacc atcctgagta
     4381 aaacagaaga atcataccca ggaggaggca tcccctgccc ggcttcatca ccagctgacg
     4441 ggtgatgtat tgtcttacat ctgctgtact ggactctgag ctccgcgagg gcagggagct
     4501 ggcattcagt aactgtatta tccccatgca ggtcagactt tggctcacag tagagacaaa
     4561 tagtatgttg aatgaataaa aattcaagtt ggtaaaaaaa aaaaaaaaaa aaaaa
//
