LOCUS       NM_001024134            1843 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus tripartite motif-containing 15 (Trim15), transcript
            variant 2, mRNA.
ACCESSION   NM_001024134 XM_110239 XM_128620 XM_289866
VERSION     NM_001024134.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Uchil,P.D., Pawliczek,T., Reynolds,T.D., Ding,S., Hinz,A.,
            Munro,J.B., Huang,F., Floyd,R.W., Yang,H., Hamilton,W.L.,
            Bewersdorf,J., Xiong,Y., Calderwood,D.A. and Mothes,W.
  TITLE     TRIM15 is a focal adhesion protein that regulates focal adhesion
            disassembly
  JOURNAL   J Cell Sci 127 (Pt 18), 3928-3942 (2014)
   PUBMED   25015296
  REMARK    GeneRIF: TRIM15-depleted cells display impaired cell migration and
            reduced focal adhesion disassembly rates.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CR956641.9.
            
            On Nov 17, 2006 this sequence version replaced NM_001024134.1.
            
            Transcript Variant: This variant (2) has an additional segment in
            the 3' CDS, as compared to variant 1, resulting in a reading frame
            shift. The resulting isoform (2) is slightly shorter and has a
            different C-terminus, as compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027186.1, SRR5189685.158836.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-452               CR956641.9         150812-151263
            453-548             CR956641.9         151599-151694
            549-779             CR956641.9         152808-153038
            780-802             CR956641.9         153728-153750
            803-918             CR956641.9         155101-155216
            919-951             CR956641.9         155640-155672
            952-1843            CR956641.9         156417-157308
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 19.16 cM"
     gene            1..1843
                     /gene="Trim15"
                     /gene_synonym="1810012B10Rik"
                     /note="tripartite motif-containing 15"
                     /db_xref="GeneID:69097"
                     /db_xref="MGI:MGI:1916347"
     exon            1..452
                     /gene="Trim15"
                     /gene_synonym="1810012B10Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..959
                     /gene="Trim15"
                     /gene_synonym="1810012B10Rik"
                     /EC_number="2.3.2.27"
                     /note="isoform 2 is encoded by transcript variant 2;
                     tripartite motif protein 15"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase TRIM15 isoform 2"
                     /protein_id="NP_001019305.1"
                     /db_xref="CCDS:CCDS50102.1"
                     /db_xref="GeneID:69097"
                     /db_xref="MGI:MGI:1916347"
                     /translation="MPSTRSLRGLHQVACPVCTRPLQDAVTTACGHTVCLLCLPRTPM
                     GAQLLCPLCQGAEEEKIQAAVAPVSLGPLSETYCEEHGEKIYFFCKTDAELLCVFCRE
                     GPAHQAHTVGFLDEAIQPYRDRLRSRLEALRMERDKIEDRKCQEDQKLQEVLMQVESK
                     KHQVEAAFERLKRELVDQRCLLLNRLEELERQICKEREEYISKVSQEVDRLSTQVEEL
                     EEKCQQPASELLQDARLNQSRYELKTFVSPEAISSDLVKKIRNLHRKILSLPKMMRTF
                     SENLMQHLETDSGS"
     exon            453..548
                     /gene="Trim15"
                     /gene_synonym="1810012B10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            549..779
                     /gene="Trim15"
                     /gene_synonym="1810012B10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            780..802
                     /gene="Trim15"
                     /gene_synonym="1810012B10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            803..918
                     /gene="Trim15"
                     /gene_synonym="1810012B10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            919..951
                     /gene="Trim15"
                     /gene_synonym="1810012B10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1843
                     /gene="Trim15"
                     /gene_synonym="1810012B10Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1825..1830
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trim15"
                     /gene_synonym="1810012B10Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1843
                     /gene="Trim15"
                     /gene_synonym="1810012B10Rik"
                     /note="major polyA site"
ORIGIN      
        1 gcacttaaaa gtgactcagt ttcagggaaa ggaaattgga gtggaccgag tcagaagact
       61 gtggacaggc aggggaaggc acggtgatgc cctcaacccg gtccctgcgg ggacttcacc
      121 aagtagcctg tcctgtctgc acccgaccct tgcaggatgc ggtgaccaca gcctgcggac
      181 acactgtctg cctactctgc ctcccgcgca ccccaatggg agcccagctg ctgtgccctc
      241 tctgtcaggg ggcagaagag gagaaaatcc aggccgcagt ggccccggtg tccctaggtc
      301 ccctgagcga gacctactgt gaagagcacg gagagaagat ctatttcttc tgtaagacag
      361 acgcggagct gctctgcgtg ttctgcaggg agggccccgc ccaccaggca cacactgtgg
      421 ggttcctgga tgaagccatc caaccctaca gggatcgtct caggagtcgg ttagaagctc
      481 taagaatgga gagggacaag atagaagata ggaaatgtca agaagaccag aagctccagg
      541 aggttctgat gcaggttgaa agcaaaaagc atcaggtaga agctgctttt gaaaggctga
      601 agcgggagct tgtggaccag agatgcctcc tgctgaacag gctggaggaa ctagaacggc
      661 agatctgcaa ggagagggaa gagtacatca gcaaagtctc ccaggaggtc gaccggctga
      721 gtacccaggt ggaggagcta gaggagaagt gtcagcaacc agcaagtgag cttctgcaag
      781 atgccagact caatcagagc aggtacgagc tgaagacttt tgtgagccca gaggccattt
      841 cctctgatct tgtcaagaag atccgaaacc tccacaggaa aatacttagc ctcccaaaga
      901 tgatgaggac attttcagaa aacttgatgc agcatctgga gacagattca ggctcctagg
      961 tactgtgctg ctgctgttct ggccacttca ctttaagaaa caaatcctca gacacagctg
     1021 tcactcctgt ccttcagaaa cagaagccta ggaagcaggc agcctggatg gatgccgctc
     1081 catcctgaag cggggaagag gtgtcactct ggacccacag accacaaagc caagtccagt
     1141 cctctctgtg attgccactt gagtggcggc tgcatcatga gaatggtccc ctaagtgtcc
     1201 agggtgaggg gatggcagga cctgagcacc aaggacattg tctgggccat aatacttttg
     1261 ctccaactgg ccagcgccca cctgccatgc aaaaggagac acagctgtcc tgcgcagtgt
     1321 agatgtgacc aggtgtggga gagaccttgc aaatgtccca caccaaggct tcagtcacca
     1381 tctttttcta accctatccc acctggaaag taagttcctc tgcctgcctt tgaaagacta
     1441 aggtggagcc agggaaatgc ggcagacaca ccagcaccct tggaacccag ctctgtccct
     1501 agcattcaaa tgggaatctt gtgtagcaag tcctttcact cctgttcctg gacacggtga
     1561 tctctctgaa tggagagcca gcagacagga aggcattgtc ctagacacca gcaaagctat
     1621 gagtcaatgg gatttggaag ggaagcaaac aaaaagctgt aaaagcttca aaatgaccac
     1681 tgtagggtaa ccaggaacct gtgacttgta ctaatttatg acttttggac acgaagctgg
     1741 agatgctaca gggcttcatc aacaactctg ggtggaccct aaagcccctt cagctcattt
     1801 atctacgttc tgagaggatg ttttaataaa atatgtaacc taa
//
