LOCUS       NM_029726               4514 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus triadin (Trdn), transcript variant 1, mRNA.
ACCESSION   NM_029726 XM_483890 XM_906658
VERSION     NM_029726.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Zhao,Y., Riching,A.S., Knight,W.E., Chi,C., Broadwell,L.J., Du,Y.,
            Abdel-Hafiz,M., Ambardekar,A.V., Irwin,D.C., Proenza,C., Xu,H.,
            Leinwand,L.A., Walker,L.A., Woulfe,K.C., Bristow,M.R.,
            Buttrick,P.M. and Song,K.
  TITLE     Cardiomyocyte-Specific Long Noncoding RNA Regulates Alternative
            Splicing of the Triadin Gene in the Heart
  JOURNAL   Circulation 146 (9), 699-714 (2022)
   PUBMED   35862102
  REMARK    GeneRIF: Cardiomyocyte-Specific Long Noncoding RNA Regulates
            Alternative Splicing of the Triadin Gene in the Heart.
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Manno,C., Tammineni,E., Figueroa,L., Marty,I. and Rios,E.
  TITLE     Quantification of the calcium signaling deficit in muscles devoid
            of triadin
  JOURNAL   PLoS One 17 (2), e0264146 (2022)
   PUBMED   35213584
  REMARK    GeneRIF: Quantification of the calcium signaling deficit in muscles
            devoid of triadin.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4514)
  AUTHORS   Seo,M.H., Lim,S. and Yeo,S.
  TITLE     Association of decreased triadin expression level with apoptosis of
            dopaminergic cells in Parkinson's disease mouse model
  JOURNAL   BMC Neurosci 22 (1), 65 (2021)
   PUBMED   34736417
  REMARK    GeneRIF: Association of decreased triadin expression level with
            apoptosis of dopaminergic cells in Parkinson's disease mouse model.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4514)
  AUTHORS   Sebastien,M., Aubin,P., Brocard,J., Brocard,J., Marty,I. and
            Faure,J.
  TITLE     Dynamics of triadin, a muscle-specific triad protein, within
            sarcoplasmic reticulum subdomains
  JOURNAL   Mol Biol Cell 31 (4), 261-272 (2020)
   PUBMED   31877066
  REMARK    GeneRIF: Dynamics of triadin, a muscle-specific triad protein,
            within sarcoplasmic reticulum subdomains.
REFERENCE   5  (bases 1 to 4514)
  AUTHORS   Zhang,L., Salgado-Somoza,A., Vausort,M., Leszek,P. and Devaux,Y.
  CONSRTM   Cardiolinctrade mark network. Electronic address:
            https://www.cardiolinc.org
  TITLE     A heart-enriched antisense long non-coding RNA regulates the
            balance between cardiac and skeletal muscle triadin
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1865 (2), 247-258 (2018)
   PUBMED   29126880
  REMARK    GeneRIF: The lncRNA TRDN-AS regulates the balance between cardiac
            and skeletal isoforms of triadin.
REFERENCE   6  (bases 1 to 4514)
  AUTHORS   Arvanitis,D.A., Vafiadaki,E., Fan,G.C., Mitton,B.A., Gregory,K.N.,
            Del Monte,F., Kontrogianni-Konstantopoulos,A., Sanoudou,D. and
            Kranias,E.G.
  TITLE     Histidine-rich Ca-binding protein interacts with sarcoplasmic
            reticulum Ca-ATPase
  JOURNAL   Am J Physiol Heart Circ Physiol 293 (3), H1581-H1589 (2007)
   PUBMED   17526652
  REMARK    GeneRIF: Histidine-rich Ca-binding protein may play a key role in
            the regulation of SR Ca cycling through its direct interactions
            with SERCA2 and triadin, mediating a fine cross talk between SR Ca
            uptake and release in the heart.
REFERENCE   7  (bases 1 to 4514)
  AUTHORS   Kirchhof,P., Klimas,J., Fabritz,L., Zwiener,M., Jones,L.R.,
            Schafers,M., Hermann,S., Boknik,P., Schmitz,W., Breithardt,G.,
            Kirchhefer,U. and Neumann,J.
  TITLE     Stress and high heart rate provoke ventricular tachycardia in mice
            expressing triadin
  JOURNAL   J Mol Cell Cardiol 42 (5), 962-971 (2007)
   PUBMED   17408688
  REMARK    GeneRIF: Triadin(TRD)xjunctin(JCN) mice were studied to compensate
            the down-regulation of junctin expression in triadin-expressing
            mice; exercise or stress provoked repetitive ventricular
            tachycardia in freely roaming TRDxJCN mice.
REFERENCE   8  (bases 1 to 4514)
  AUTHORS   Hong,S., Kim,T.W., Choi,I., Woo,J.M., Oh,J., Park,W.J., Kim,D.H.
            and Cho,C.
  TITLE     Complementary DNA cloning, genomic characterization and expression
            analysis of a mammalian gene encoding histidine-rich calcium
            binding protein
  JOURNAL   Biochim Biophys Acta 1727 (3), 188-196 (2005)
   PUBMED   15777620
REFERENCE   9  (bases 1 to 4514)
  AUTHORS   Kirchhefer,U., Baba,H.A., Kobayashi,Y.M., Jones,L.R., Schmitz,W.
            and Neumann,J.
  TITLE     Altered function in atrium of transgenic mice overexpressing
            triadin 1
  JOURNAL   Am J Physiol Heart Circ Physiol 283 (4), H1334-H1343 (2002)
   PUBMED   12234783
  REMARK    GeneRIF: triadin 1 overexpression triggered time-dependent
            alterations in SR protein expression, Ca(2+) homeostasis, and
            contractility, indicating for the first time an inhibitory function
            of triadin 1 on SR-Ca(2+) release in vivo.
REFERENCE   10 (bases 1 to 4514)
  AUTHORS   Hong,C.S., Ji,J.H., Kim,J.P., Jung,D.H. and Kim,D.H.
  TITLE     Molecular cloning and characterization of mouse cardiac triadin
            isoforms
  JOURNAL   Gene 278 (1-2), 193-199 (2001)
   PUBMED   11707337
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB658668.1, AK009816.1, AC157017.3, AI642378.1, AC153966.4,
            AC109214.6 and AK036470.1.
            
            On or before Apr 9, 2009 this sequence version replaced
            XM_906658.3, XM_483890.5, NM_029726.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMN01164133, SAMN01766820
                              [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                BB658668.1         3-32
            31-791              AK009816.1         4-764
            792-851             AC157017.3         90125-90184
            852-1088            AI642378.1         3-239
            1089-1160           AC157017.3         137339-137410
            1161-1223           AC157017.3         160559-160621
            1224-1277           AC157017.3         161571-161624
            1278-1331           AC153966.4         134402-134455
            1332-1361           AC153966.4         150670-150699
            1362-1391           AC109214.6         12646-12675
            1392-1409           AC109214.6         13442-13459
            1410-1442           AC109214.6         15192-15224
            1443-1469           AC109214.6         15350-15376
            1470-1707           AK036470.1         9-246
            1708-4514           AK036470.1         248-3054
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 18.37 cM"
     gene            1..4514
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /note="triadin"
                     /db_xref="GeneID:76757"
                     /db_xref="MGI:MGI:1924007"
     exon            1..263
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     CDS             242..2323
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cardiac triadin"
                     /codon_start=1
                     /product="triadin isoform 1"
                     /protein_id="NP_084002.2"
                     /db_xref="CCDS:CCDS48532.1"
                     /db_xref="GeneID:76757"
                     /db_xref="MGI:MGI:1924007"
                     /translation="MTEITAEGNASTTTTVIDNKNGCIPKSPGKVLKRSVTEDIVTTF
                     SSPAAWLLVIALIITWSAVAIVMFDLVDYKNFSASSIAKIGSDPLKLVNDAVEETTDW
                     IYGFFSLLSDIISSEGDEDDEDADEDIDKGEIEEPPLKRKEIHQEKAEKEEKPEKKIQ
                     TKASHREREKGKEKLKGEKPEKTATHKEKLEKKERPETKMMAKEDKKIKTKEKTEEKA
                     KKEMKVGKQEKVKPTAAKAKETPKTPPKARKKDDKEMPAVHEQKDQYAFCRYMIDMFV
                     HGDLKPGQSPVMPPPSLTPSKPALSTTALEEKEKEEKKKMEKKDTSDTKKKEKEVKKK
                     SEETTIDGKGKEPGKPPETKQMTAKLTTQAAARKDEKKEESKKMRKPTEEQPKGKKQE
                     KKEKHIEPAKTPKKEHPGPSEKLKKAKAEQAKEEIAAASTKKALHGKKEEKAKTVEQE
                     VKKEKSGKSSSDLKDKEVKKEKSGKSSSDLKDKEPQLKNEEKSKPQVKKEAKLASSDK
                     GQTRKQNITRPEQVIPHVKPEKAEHQEKGHPSIKKDKPKPSSKGAPEVPDSGKKKIEK
                     SEKESKVPTREENLQVYNVTKAEKPGKIPKDSKEAPASKKDKEDSKEAPTSKKDKEDS
                     KDVPHSKKDKEVTDDVSSPKKQTRPISFFQCVYLNGYNGYGFQFPVTPVQQPGENPGK
                     TNSPGQKQQEQ"
     exon            264..473
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            474..635
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            636..668
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            669..728
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            729..791
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            792..851
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            852..1028
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1088
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1160
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1223
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1277
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1331
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1361
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1391
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1409
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1442
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1443..1469
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..1496
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1497..1544
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..1589
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1640
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1691
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1721
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1751
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1778
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1826
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1827..1871
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1872..1892
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1893..1919
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..1958
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            1959..2003
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2027
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            2028..2066
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2105
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            2106..2144
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            2145..2183
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     exon            2184..4514
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4430..4435
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /note="hexamer: AGTAAA"
     polyA_site      4452
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /note="major polyA site"
     regulatory      4475..4480
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
                     /note="hexamer: AAGAAA"
     polyA_site      4510
                     /gene="Trdn"
                     /gene_synonym="2310045H21Rik; EG432451; TDN"
ORIGIN      
        1 gtttgaagct gatactttta gctgagccac aagtactgct tggaaagggc ttaggttgtt
       61 tccaggggga gaactctgca gagaagaggc aaagttcaac aagacaacaa aaatcagcca
      121 gaaaatccag gaaaaggaag agcccttgtc atacccctct accagagttc tctaatccta
      181 ccttattctg caaagtgcca cttgactgga actcttcacc tgcttgattt ctgcacccac
      241 catgactgag atcactgctg aaggaaatgc atcgacaacc acaacggtga tagacaacaa
      301 aaatggatgt attcctaaat cccctggaaa ggtgctgaag aggtctgtca ccgaagacat
      361 tgtgacaaca ttcagctccc ctgcagcctg gcttcttgtc atcgctctga ttatcacatg
      421 gtcagctgtt gctatcgtga tgtttgattt agtggattat aaaaactttt cagcaagctc
      481 cattgccaag attggctcag atcctctaaa actagtgaat gatgctgtgg aggagacaac
      541 agactggatc tatggcttct tctctttgct atctgacatc atctcatctg aaggtgacga
      601 agatgatgag gatgcagatg aagacattga taaaggagaa atagaagaac ctcccttaaa
      661 aagaaaagaa atacaccaag aaaaggctga aaaagaggag aaacctgaga agaaaataca
      721 aactaaagct tcacacagag aaagggagaa aggaaaagaa aaattaaaag gagaaaaacc
      781 tgagaagaca gcaactcaca aagagaaact tgagaaaaaa gaaagaccag agacgaagat
      841 gatggcaaaa gaggacaaga aaattaagac taaggaaaag actgaagaaa aggctaagaa
      901 ggaaatgaaa gttggaaaac aggagaaagt gaaaccaaca gctgcaaaag ccaaagaaac
      961 tccgaaaaca ccaccaaagg ccagaaagaa ggatgacaaa gagatgccag ctgtgcatga
     1021 gcagaaagat cagtatgcat tctgtcgata tatgattgac atgtttgtcc atggggattt
     1081 aaaaccagga caaagcccag ttatgccacc accatcattg acaccttcca aacctgcttt
     1141 gtcaacaacg gcccttgaag aaaaagaaaa agaagagaag aagaaaatgg agaagaaaga
     1201 tacttctgat acaaaaaaga aagaaaaaga agtcaaaaag aaaagtgaag aaactaccat
     1261 tgatgggaaa ggcaaagaac caggcaaacc tcctgaaacc aaacaaatga ccgccaaact
     1321 cacaacacaa gctgcagcta gaaaggatga gaagaaagaa gaatcaaaga aaatgagaaa
     1381 acctacagaa gaacaaccca agggaaaaaa acaggaaaag aaagaaaagc acatagaacc
     1441 agcaaagaca ccaaaaaagg aacatccagg tccaagtgaa aaactcaaaa aagcaaaagc
     1501 tgaacaagcc aaggaagaga ttgctgctgc ctcaacaaaa aaggccctgc atggaaagaa
     1561 agaagagaaa gccaagacag tcgagcaaga agtgaaaaaa gaaaaatctg ggaagagttc
     1621 ttcagatttg aaagataaag aagtgaaaaa agaaaagtct gggaagagtt cttcagattt
     1681 gaaagataaa gagccacaac taaaaaacga agagaagtca aagccacaag tgaaaaagga
     1741 agcaaaacta gcatcatctg acaaaggcca aacacgcaaa cagaacatca ccagaccaga
     1801 gcaagttatt cctcatgtta aaccagaaaa agctgaacac caagaaaaag gccacccatc
     1861 tataaaaaaa gataagccta aaccatcttc aaagggagca ccagaagttc cagactcagg
     1921 gaagaagaag attgaaaaat ctgaaaagga aagtaaagtt ccaacaagag aagaaaatct
     1981 tcaggtgtac aatgtgacta aagcagaaaa acctggaaaa ataccaaaag attccaaaga
     2041 agcaccagct tcaaagaaag ataaagaaga ttccaaagaa gcaccaactt caaagaaaga
     2101 taaagaagac tccaaagatg tcccacattc aaagaaagat aaagaagtaa ctgatgatgt
     2161 atcttcacca aagaagcaaa cacgtcccat cagcttcttc caatgtgtgt acttgaatgg
     2221 gtacaatggc tatgggtttc agtttcctgt cactcctgtc caacaacctg gagagaaccc
     2281 tggcaaaaca aactctccag gacagaagca acaagaacaa tagacatcca cgcctgaccc
     2341 tgacaactgc ttttacatgg tgaaagtgga atgttctctt ttgtccacgg tggcacaggc
     2401 aatcaaaact atctgatgct gagattccat tgtaaacatt ttgcctggca taaagagatg
     2461 acatagacaa aggaattgac taataagcaa ttgtgttcag ggtctaatac ttgagaatct
     2521 tcatttgtgt ctgttgctat aggagggata cttacattaa gctccttcta gctgttataa
     2581 ggtagtatgt tcattaaagt ttacttttaa atataattgg cacaattaca aaatgagatt
     2641 tatgatttca ggcagggaat ttctaaattg gtaacataga ctgtgttcta attttaaata
     2701 gcacaggaac atatataaaa gttattcttc tgcatcatgt gggtagactc agtttaccca
     2761 aaaaaaccta tgtgcatcat tatattcacg tctgtttcta gttcagttat ggcaaggatc
     2821 tacataggta tatcttacac cagagtgctc taaatccctt gcaatattct taatgatcag
     2881 actatctaaa agatgggaca tgacaaagaa taagtgcttg ttggatctta tctctgaggt
     2941 tgtcttgggc tatgcttaag acattctctc ttgtggacat tgcaattaag cacatttggt
     3001 aaataacttg tttaatatgc aagttaatga gatatgaaat tttcacatct ctccagattg
     3061 tgcctcacta aatccatcac ctggatgaag gtttagttca aagaacaaca actatggttg
     3121 atcaatccaa acagttattt ctcaatacat actttctgtg agattagatg ttttcttttt
     3181 gaaatggaaa caatacaaat gacataaaac gttaagtgag atattttggg tccatacatg
     3241 ttcatgagat gtcttacttt cttctggcta attgaacagt aatgccagtt aaatgatggc
     3301 agtttttaac ttacttgtcc atgcatcact gaaaataact gttgagaatg ttcatccttt
     3361 tggtaataca aacactagat tttcatgaac ttttcttatc tagaaaacat aattgaatct
     3421 tttcatcatg tttctacttg cagctgaact tatgacaaat taactcacaa aacaacttga
     3481 gttaccattt ctggcactat cataagctca gccaatgctg agctactgac tttgtgaact
     3541 agctgtgtga ttggtttcaa acactgcagc caaacatagt tcttgaggtc taagacaaaa
     3601 gtccatttta tcaatattca ggtaggatat ggaccatgta cttagcatta acgatgggat
     3661 atcggtgact tttataagaa agttagcttc aaccagatgc tgacagactg aattcacacc
     3721 aaaaatgtca acactacaat gaactagatt aatggagcca tttggagggg aaggagtaca
     3781 taattgacca gagcttcatt caacaattca aacttacaag gagaacagtg gataaagtgc
     3841 tctacttagt tgaagtttca ctaatttcct gagtatggga cagagaataa atgtgtttta
     3901 aaacacatac taccaaatgg gttatccact aagttattat ctttcaatga ctatgcactt
     3961 agtctttcta tactgtttca ggttatagac catgaaacat agtacagcaa tagattctac
     4021 ttcatgttat tctggcacag taagtaacat tcaaaaaatg cattatatgt attaggtctg
     4081 tatctccagg tttcaaagaa ccatcaaaac aaacagctca gcacttcaca gagttactct
     4141 gtagctagat aaagtgtctt aatacgggtt tgtttgaaag cattctttga aattatgttt
     4201 ttggagatta cattaatctt atctctacca acttttaaat atcatcatct attttctttg
     4261 tgaaacaaat tgacaaaata taacaaatgt tattgtaaat ttgggtgtga gttagagaaa
     4321 aaaatatgat gtaatagtta acaatagttg ttaagatttg tctaaaattt gaataactag
     4381 tctaatttga aaaaattatc taaactgttt tctgttatat ttttacatta gtaaactact
     4441 tctgactctt gagtttaatg gtttgtatct tgaaaagaaa aggcatgaat agttaatttt
     4501 ttattttaaa tttc
//
