LOCUS       NM_010067               7919 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus tRNA aspartic acid methyltransferase 1 (Trdmt1),
            transcript variant 1, mRNA.
ACCESSION   NM_010067
VERSION     NM_010067.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7919)
  AUTHORS   Chen,X., Wang,C., Zhao,G., Li,Z., Zhang,W., Song,T., Zhang,C. and
            Duan,N.
  TITLE     Suppression of DNMT2/3 by proinflammatory cytokines inhibits
            CtBP1/2-dependent genes to promote the occurrence of atrophic
            nonunion
  JOURNAL   Cytokine 173, 156436 (2024)
   PUBMED   37979214
  REMARK    GeneRIF: Suppression of DNMT2/3 by proinflammatory cytokines
            inhibits CtBP1/2-dependent genes to promote the occurrence of
            atrophic nonunion.
REFERENCE   3  (bases 1 to 7919)
  AUTHORS   Satir-Basaran,G., Kianmehr,L., Mehmetbeyoglu,E., Korkmaz Bayram,K.,
            Memis,M., Yilmaz,Z., Tufan,E., Taheri,S., Kelestimur,F. and
            Rassoulzadegan,M.
  TITLE     Mouse Paternal RNAs Initiate a Pattern of Metabolic Disorders in a
            Line-Dependent Manner
  JOURNAL   Front Genet 13, 839841 (2022)
   PUBMED   35419033
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7919)
  AUTHORS   Yu,T., Xie,Y., Tang,C., Wang,Y., Yuan,S., Zheng,H. and Yan,W.
  TITLE     Dnmt2-null sperm block maternal transmission of a paramutant
            phenotypedagger
  JOURNAL   Biol Reprod 105 (3), 603-612 (2021)
   PUBMED   33929014
  REMARK    GeneRIF: Dnmt2-null sperm block maternal transmission of a
            paramutant phenotypedagger.
REFERENCE   5  (bases 1 to 7919)
  AUTHORS   Muller,M., Legrand,C., Tuorto,F., Kelly,V.P., Atlasi,Y., Lyko,F.
            and Ehrenhofer-Murray,A.E.
  TITLE     Queuine links translational control in eukaryotes to a
            micronutrient from bacteria
  JOURNAL   Nucleic Acids Res 47 (7), 3711-3727 (2019)
   PUBMED   30715423
  REMARK    GeneRIF: We also show that Q-dependent stimulation of Dnmt2 is
            conserved in mice. Our findings reveal a direct mechanism for the
            regulation of translational speed and fidelity in eukaryotes by a
            nutrient originating from bacteria.
REFERENCE   6  (bases 1 to 7919)
  AUTHORS   MacDonald,J.L., Gin,C.S. and Roskams,A.J.
  TITLE     Stage-specific induction of DNA methyltransferases in olfactory
            receptor neuron development
  JOURNAL   Dev Biol 288 (2), 461-473 (2005)
   PUBMED   16288735
REFERENCE   7  (bases 1 to 7919)
  AUTHORS   Chen,H.W., Chen,J.J., Yu,S.L., Li,H.N., Yang,P.C., Su,C.M.,
            Au,H.K., Chang,C.W., Chien,L.W., Chen,C.S. and Tzeng,C.R.
  TITLE     Transcriptome analysis in blastocyst hatching by cDNA microarray
  JOURNAL   Hum Reprod 20 (9), 2492-2501 (2005)
   PUBMED   15919778
REFERENCE   8  (bases 1 to 7919)
  AUTHORS   Dong,A., Yoder,J.A., Zhang,X., Zhou,L., Bestor,T.H. and Cheng,X.
  TITLE     Structure of human DNMT2, an enigmatic DNA methyltransferase
            homolog that displays denaturant-resistant binding to DNA
  JOURNAL   Nucleic Acids Res 29 (2), 439-448 (2001)
   PUBMED   11139614
REFERENCE   9  (bases 1 to 7919)
  AUTHORS   Okano,M., Xie,S. and Li,E.
  TITLE     Dnmt2 is not required for de novo and maintenance methylation of
            viral DNA in embryonic stem cells
  JOURNAL   Nucleic Acids Res 26 (11), 2536-2540 (1998)
   PUBMED   9592134
REFERENCE   10 (bases 1 to 7919)
  AUTHORS   Yoder,J.A. and Bestor,T.H.
  TITLE     A candidate mammalian DNA methyltransferase related to pmt1p of
            fission yeast
  JOURNAL   Hum Mol Genet 7 (2), 279-284 (1998)
   PUBMED   9425235
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL772303.11 and AL773538.7.
            
            On Feb 23, 2023 this sequence version replaced NM_010067.4.
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029136.1, BC087892.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-96                AL772303.11        17773-17868         c
            97-206              AL772303.11        6588-6697           c
            207-283             AL773538.7         175366-175442       c
            284-355             AL773538.7         173174-173245       c
            356-421             AL773538.7         172572-172637       c
            422-491             AL773538.7         171826-171895       c
            492-575             AL773538.7         171011-171094       c
            576-919             AL773538.7         169504-169847       c
            920-977             AL773538.7         166407-166464       c
            978-1107            AL773538.7         165763-165892       c
            1108-7919           AL773538.7         154627-161438       c
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 10.0 cM"
     gene            1..7919
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /note="tRNA aspartic acid methyltransferase 1"
                     /db_xref="GeneID:13434"
                     /db_xref="MGI:MGI:1274787"
     exon            1..96
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /inference="alignment:Splign:2.1.0"
     CDS             33..1280
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /EC_number="2.1.1.204"
                     /note="isoform 1 is encoded by transcript variant 1; tRNA
                     (cytosine-5-)-methyltransferase; tRNA
                     (cytosine(38)-C(5))-methyltransferase; DNA MTase homolog
                     MmuIIP; DNA methyltransferase homolog MmuIIP; DNA
                     (cytosine-5)-methyltransferase-like protein 2; DNA
                     methyltransferase 2"
                     /codon_start=1
                     /product="tRNA (cytosine(38)-C(5))-methyltransferase
                     isoform 1"
                     /protein_id="NP_034197.3"
                     /db_xref="CCDS:CCDS15695.1"
                     /db_xref="GeneID:13434"
                     /db_xref="MGI:MGI:1274787"
                     /translation="MEPLRVLELYSGIGGMHHALRESHIPAHVVAAIDVNTVANEVYK
                     HNFPHTHLLSKTIEGISLEDFDKLSFNMILMSPPCQPFTRIGLQGDMTDPRTTSFLYI
                     LDILPRLQKLPKYILLENVKGFEVSSTRGLLIQTIEACGFQYQEFLLSPSSLGIPNSR
                     LRYFLIAKLQSEPFPFQAPGQILMEFPKIVTVEPQKYAVVEESQPRVQRTGPRICAES
                     SSTQSSGKDTILFKLETVEERDRKHQQDSDLSVQMLKDFLEDGDTDEYLLPPKLLLRY
                     ALLLDIVKPTSRRSMCFTKGYGSYIEGTGSVLQAAEDAQIENIYKSLPDLPPEEKIAK
                     LSMLKLRYFTPKEIANLQGFPPEFGFPEKTTVKQRYRLLGNSLNVHVVAKLLTVLCEG
                     FGNASESCHKMPLILDSNSKILS"
     exon            97..206
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            207..283
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            284..355
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            356..421
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            422..491
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            492..575
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            576..919
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            920..977
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1107
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..7919
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1665..1670
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /note="hexamer: TATAAA"
     polyA_site      1687
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /note="major polyA site"
     regulatory      2019..2024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /note="hexamer: ATTAAA"
     polyA_site      2046
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /note="major polyA site"
     regulatory      2612..2617
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /note="hexamer: ATTAAA"
     polyA_site      2637
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /note="major polyA site"
     regulatory      3757..3762
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /note="hexamer: ATTAAA"
     polyA_site      3784
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
     regulatory      6456..6461
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /note="hexamer: AATAAA"
     polyA_site      6476
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
     regulatory      7895..7900
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
                     /note="hexamer: AATAAA"
     polyA_site      7919
                     /gene="Trdmt1"
                     /gene_synonym="Dnmt2; m.MmuIIP; met-2; Rnmt2"
ORIGIN      
        1 agtttccagg ccgcggtcgc ggttgcgaga ggatggaacc tctgcgtgtc ctggagctgt
       61 acagtggcat tggtggcatg caccacgcgc tgcgagaaag tcatatccct gcacatgtgg
      121 tggctgctat tgatgtgaat actgttgcta atgaagtata caagcataat tttcctcata
      181 ctcacttact gtcaaagaca attgaaggta tttcactgga agactttgac aagctatctt
      241 tcaatatgat tttaatgagc cctccatgcc agccattcac aagaattggc ctacaggggg
      301 atatgaccga tccaaggaca actagcttct tgtatattct agatattctc ccaagattac
      361 aaaaattacc caagtatatt ctcttagaaa atgtcaaagg ttttgaagta tcttctacaa
      421 gagggctgct gatacaaaca atagaagcct gtggctttca gtatcaagag tttctattgt
      481 ctccttcttc tctaggcatt ccaaactcga ggctccgata ttttctcatt gcaaagcttc
      541 agtcagagcc tttccccttc caggcccctg gacagatact gatggagttt cctaaaattg
      601 taactgttga gccacaaaaa tatgcagtag ttgaagaaag tcagccaaga gtgcagagaa
      661 ctggaccacg tatctgtgct gagagcagca gcacacagag ttctggaaaa gataccattc
      721 tctttaagct tgagactgta gaagaaaggg acaggaaaca tcaacaagac agtgacctct
      781 ctgtgcagat gctgaaagac ttccttgaag atggcgacac agatgagtac cttttaccac
      841 ccaagttatt gctgcgatac gctctcttac tagatatcgt gaagcccacg tccagaaggt
      901 ccatgtgctt tacgaaaggg tatgggagtt acatagaggg gacaggctcc gtgttacagg
      961 ctgcagagga tgcgcagatt gagaatatct acaaatctct tcctgatttg ccaccagaag
     1021 aaaagatagc taaattgtca atgcttaaac tgcgatattt cacaccgaaa gaaattgcaa
     1081 atctccaggg atttcctcca gaatttgggt ttcctgagaa gacaacagtg aaacagcgtt
     1141 accggctgct tggcaatagc ctcaacgtgc atgtggtagc aaaactcctc acagtcctgt
     1201 gtgaaggatt tggaaatgcc tctgagagct gtcacaagat gccgctaatt ctagatagta
     1261 attccaagat tctatcttga atgaattctt atagagttca gctaaattct ttgaatagca
     1321 ttttcctggg taattcagca ggaacttaaa tgtgtacatc caattgtccc tcacatttat
     1381 gtcactgtat catccaattg tccctcacat ttatgtcact gtatcatcca attgtccctc
     1441 acatttatgt cactgtatca tccaattgtc ccttacattt atgtcactgt atcatccaat
     1501 tgtcccttac atttatgtca ctggatttga taacttcaca ttgctggttt tgctttatgg
     1561 acaaatattt ttacaaagct tttaaacttg tgtattaaat atcttttata ttatgttata
     1621 aacaaattta agaatgcttg aattttatat gtatattagt actttataaa gtatgacatg
     1681 aataatataa tgatccttag aaaattattt ttgtatgtca atttaaaaat tgttttatat
     1741 aacatggaga aactaactgg atatagatgg aagcttcatc cctctagcta gtgctgttca
     1801 tgctaccaga agcaaaaata gccatcatgt ttagctagct atgaaccagg acgccacagt
     1861 agtgactctc ctacgaagat atgcccatga gtatcgtggg aatagccacc tgctctgtga
     1921 tacgatttaa ggcctgatca atggctcttt tatctggttc agagacctgt ggctagacag
     1981 gttatgggcc ccagtaagta acttactagt attattttat taaatgggca ttgtattaaa
     2041 tgataatgat tcttgatgat tgattcttat accatagatt agtgcgtccc tcaacattca
     2101 acaggtaagc aagcctcttg cagtagacag caacacagaa acccagagag caagagactg
     2161 tgaaggactc agcgctaaga gacatctatc actcccctcc ctcccgaggc tcagcgatca
     2221 ttgcagaaga ggaagaaaaa acactgtaag agtcacaagt ggtagatgac tttaggaaaa
     2281 tatattatcc agatcagcag gacaactaca cgtgtggact catagctagg tgacagcatt
     2341 caaaagatct gaaccccaga gcgcatccca gcatggagca ggtagagtgg gtaccaaggc
     2401 acacccctag ctcagaggct attggcattt ggtagctgct gggagaggaa cctaagatgt
     2461 gattcctggt atgtggaaca caccccaggg taggcttata ctcaagagta tttgaggaac
     2521 acaaattgaa tttgggtgga taggggaggg agtatgaata tgatccaaat aaatcgtatg
     2581 acattctcaa agaattaata ttatttaaaa aattaaaaat tgttttacaa ctcttaagat
     2641 attactcttt ttgttaattt atcctaactc aaaactgact caaatgctag ctattgagtg
     2701 ttttatttaa tttcaggcct ttttagataa gtgcatacat tttaaaccaa attatcatca
     2761 tccaaacata gatatcagtc tgaagaatgt actagaaaaa tgctcataaa ttcttaatca
     2821 aatctttgaa aactgcaggt gacctttttg tttgtttgtt tgtttgagac agggtctcac
     2881 tgtgtagccc tgggtggcct ggaagttgct atctaatcca gtctggcttc taactcaggg
     2941 atctgtctgc cccaacctcc catgtgctag ctagaattaa agctagagat acttaacctg
     3001 gcttcctctc taaccttaat ctggagataa agcctcattg tcctgcctcc atttgcacat
     3061 ccttcctttc agtttgctat taccagggtg ctttggcttt gtcagccttc ggattaagct
     3121 tctctgttgt tccattctaa ggcatatggc atgggtcatg tggcagtaag gttaagggag
     3181 gaaaacgtgc tgctattgct gctatttcta tatgatgcta ctccgtcctt tatcatactg
     3241 actctaaact taacttttct ctctccactc agtaaaagaa aagataaata aaatgtgttg
     3301 caaacatcta ctagaatacc agtcagtgtt acctggaatg ggtttatgat acttgccgtg
     3361 gcctgatgag ccttgggaaa ctgccaatga aattagcccg tcatgaaaag gcaagagtgg
     3421 tttcattccc ctgtgagtca agctcataga gacagtaagg agggcagtgt ttgcaggggt
     3481 tgggaggagg ggactaggag gtcagtgttt agcaggtaca gagcttctgt ttgcaagcat
     3541 gagaaaattc tgaaggcgag aggcactgct ggttggacat ggtggtgtgc tgagtgccac
     3601 ccaactgtac acttaatgtc tgttggatgg ctaattttat attacttaca ttgtactgaa
     3661 cttttaaaga ttactcctga actcagataa atatgggtct atcaaaggta taagctatgt
     3721 gtatatcaac agtcactgtc taaagagatg aacctcatta aagtctcaca attacaacct
     3781 gaaatcacca ttgtgaaact tactttcttt ctttcttcct ttctttcttc cttccttcct
     3841 tccttccttc cttccttcct tccttccttt cttccttcct tccttccttc ctccctccct
     3901 ccctccctcc ctccttcctt ccttccttcc ttcccctttt aaatttattt aatgtatgtg
     3961 agtatactgg tctctgtctt gagagaccag aagagagcat tggatcccat tacagatgat
     4021 tgtgagccac catgtggttg ctgggaattg aactcaggac ctctggaaga gcagtagtgc
     4081 tcttaaccgc tgagccatat ctccagccca ttcatggtgc aatttcatgt aggttaactt
     4141 cactgctgaa gaattattat caaaaaaatg gtttatggtt taacagtatt atgctatgct
     4201 tacaattatg ttgtaattat aaggtccaat gccccattga acaatattat aataagtgct
     4261 tagttgtagt tcctccatta tgaacacata taggtctgca gagcatttac ttataagttg
     4321 ggaaacaaaa tatggctttg taagattttt aaggggctgg agaggtgggt cagcagttca
     4381 cagcattcac tgttctttcc agggactggg gtttgattcc taccacgtat gtgctatgct
     4441 atgtaattct aaggtactta tttcttgtcc cataattttg gtgctttaca ggccccaaac
     4501 taatgaatag atgcagatta attaatacaa acaagatgat ttatgttgga aaataacttg
     4561 gaagatgagt agtctgagga gtgaaacctg gaccaccgtt attgtcagcg tgaagtgcaa
     4621 ggatagaggg agagggaatg agtcacctct tccatgggac cagctgacct tctccagagg
     4681 acctagcaca tttgagttct ggctattaag ccacagctac aattgctctt gcctggcaaa
     4741 gtgagcagat acctgcagct gccaacccag tttgctctcc tcaacagctt ttgaaagaaa
     4801 tttgcagttg aaataataaa tgatgaaggg tttacccagt gctaaaaata ctaggatagg
     4861 gctggtgaga tggctcagtg ggtaagagca cccgactgct cttccgaagg tccagagttc
     4921 aaatcccagc aaccacatgg tggctcacaa ccatccgcaa caagatctga ttccctcttc
     4981 tggagtgtct gaggacagct acagctacag tgtacttaca tatattaata aaataaatct
     5041 ttaaaaaaaa taaataaaaa tactaggata aactctttgg aatggtccac tttctctctc
     5101 tctcccttcc tctccccctc ctctctcctt tttccctcct tccctccttc ctttcctcgc
     5161 ttcctccctt cctttttgaa gcagtgtctc tttatagttg aaactaaccg caagcttgca
     5221 atgtagatga gagtggcctt gaactttggc tccttcttcc tctgctccca gatgctggga
     5281 ccacagggta aggtgcacca tgcctagctt atgaggtgca gaggatgtgt ttagttgata
     5341 gcatgaatgt gaggtgaaca tgacccagag aagtgcaccc ctggaccact ttggcttttt
     5401 aaagaggtaa atgtatttga tggagaagct gttcatgact ctaacaaccg gcactcctac
     5461 taagaaccca tacaatgatg ggcggtgcca agcccaatgt agacaattct ggtcgcatag
     5521 ttggagggta gggttcttga aaataaatta catcctgtct ttggcttaaa tctcagcctc
     5581 attcagatca aaatgttagc taggaggatt ctgaggagga agaaatgaca ccaaggagga
     5641 ttaaaactgg tgtagttctg ttagcttagc ccagaataaa agagacgtta tgttcagccg
     5701 tcagcacgtg tgtcctgagt gatgcacttt gcaggtgagt tcttctttct ccatccaaac
     5761 caaagggatt ctgcttattg gtttgttttg gcaacttctt tatgcagagt cgagaggttt
     5821 tcacaaaccg agtcaaaaca aggtaccagt tttgagctca catcaacttt ttaagtgtgc
     5881 tttcctgtct tccttaagtt gttgggcaga aacttagctg gtgttggggt tgtaccttaa
     5941 tgacagagtg tgcagtctaa atctcagctt acacacacac acacacacac acacacacta
     6001 taaaaagaag gcacataaaa agaagaattt acatgtaatt tgctcttcac ctgggaacaa
     6061 ttttaaacac ctaacttgct ttaagtgcca ctgccctcag ctttgggctt tgagaatgag
     6121 tcacagtttt tgggagcctg tgatacactg gtttggattc ttttaaagat gtggcctttc
     6181 ggccccttga caagttcaac ctttctgcca gcatttagtg cctgtgtgca ctgcatacga
     6241 tcatgccaac ttaacaagaa tctctcatgt tctgattatt tttcctcatt cttcaactct
     6301 atctagcatg tgcctgtaag tcgtcatctg gaagagcact tcacatgctg tgctagataa
     6361 acatctagac actttccaac atcagcttct aacagccaac cgatgtccac catgtttcct
     6421 gttcttctgt tcactactaa aatgttgaag tttctaataa aaattatttt ccctgagtgc
     6481 ttctatttct ttgctacttg aaggatattt tactatagga tatgtgtact cttctaaata
     6541 tataatcaca attcaaacta tcagtttatc ctggttaatt tacctcagga attaaaatat
     6601 tcaggtggac atggtggcac acacctttaa tcccagcact agagagacag atggatctct
     6661 gtgccttccg tgccagccag gtctacttga tatgtagaga gaccctgtct caaaggaaga
     6721 aaattaaaat attctttaaa tacactacat agacctataa aataataaat aagaaaagca
     6781 tgcccctcag tacatattaa ttccatttct gagaccttag tgttaacact gaggaggcaa
     6841 gcaccagtca cttggaggga aatatacatt ctgtaagaag tcctgtggag tcatcatttg
     6901 ctcttacagt ggattctgca caaaccctag atgaggagag ggtcggttcc tctggattcc
     6961 tgatacgaag ctgtccttga gcctagttac caggcaagag gatctctgct atcagaacct
     7021 ccacaaaggc cctggagaag agttcaggca acgctcaata gtatctaagc aagttacatg
     7081 gggcagattt cagcatccta gcctacttta tcaaagaaac tccttagaaa ctatcagata
     7141 atgtctaggg tttaggcaaa tagcaattaa aattttaaag ttgttcgata cattcatctt
     7201 acttagatat gactagtttt tgttactttg tttctaattt catctacgca gcatttcatt
     7261 ctcagttgta aaattcaaaa aataacattt ataccaaata ttgataaatg agctactaga
     7321 aaattagaat gaattctagt gttctgttga ccaggactac agtgaactta gaaataacct
     7381 ggtcttttgg aacattgctt gttgtaagct ttgcatattg tatctagttt gactggtttt
     7441 gtcctgttgt tgaggcattc tctagtgtct cttgaatatt caggttctcc ctccccattg
     7501 ctggtgagac aagaaattct taaggagtat atgctggatt ttttttcctg tgttttttgt
     7561 ttgctcttaa tttatgggtc atttttgaga tgttctactc atttttggac cgtagttaaa
     7621 tcctgaggcc acttgaaagc aagctggata gtgaggctct acgttactgc atagtaacac
     7681 gccacccagg cccaacctct ggtaaggtgc acgtgaattc tccagtatag tgggtttcag
     7741 atccataaat attttgttac atacatatag agaaccttgc ttttaccttg ctactgaaca
     7801 ttattctctt ttgtttctgt tagctttgca tttagttgct caagaaacac tggcttgtgc
     7861 aaaatgctgt gttgccttct cttccttgtt ctcaaataaa tgtttttgca tttactgta
//
