LOCUS       NM_177709               3237 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus trafficking regulator of GLUT4 (SLC2A4) 1 (Trarg1),
            mRNA.
ACCESSION   NM_177709
VERSION     NM_177709.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Duan,X., Krycer,J.R., Cooke,K.C., Yang,G., James,D.E. and
            Fazakerley,D.J.
  TITLE     Membrane Topology of Trafficking Regulator of GLUT4 1 (TRARG1)
  JOURNAL   Biochemistry 57 (26), 3606-3615 (2018)
   PUBMED   29787242
  REMARK    GeneRIF: The study uses a combination of bioinformatics prediction
            tools and biochemical assays to define the membrane topology of the
            173-amino acid mouse TRARG1. These analyses revealed that, contrary
            to the consensus prediction, the N-terminus is cytosolic and that a
            short segment at the C-terminus resides in the
            luminal/extracellular space.
REFERENCE   3  (bases 1 to 3237)
  AUTHORS   Fazakerley,D.J., Naghiloo,S., Chaudhuri,R., Koumanov,F.,
            Burchfield,J.G., Thomas,K.C., Krycer,J.R., Prior,M.J., Parker,B.L.,
            Murrow,B.A., Stockli,J., Meoli,C.C., Holman,G.D. and James,D.E.
  TITLE     Proteomic Analysis of GLUT4 Storage Vesicles Reveals Tumor
            Suppressor Candidate 5 (TUSC5) as a Novel Regulator of Insulin
            Action in Adipocytes
  JOURNAL   J Biol Chem 290 (39), 23528-23542 (2015)
   PUBMED   26240143
REFERENCE   4  (bases 1 to 3237)
  AUTHORS   Beaton,N., Rudigier,C., Moest,H., Muller,S., Mrosek,N., Roder,E.,
            Rudofsky,G., Rulicke,T., Ukropec,J., Ukropcova,B., Augustin,R.,
            Neubauer,H. and Wolfrum,C.
  TITLE     TUSC5 regulates insulin-mediated adipose tissue glucose uptake by
            modulation of GLUT4 recycling
  JOURNAL   Mol Metab 4 (11), 795-810 (2015)
   PUBMED   26629404
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3237)
  AUTHORS   Sallman Almen,M., Bringeland,N., Fredriksson,R. and Schioth,H.B.
  TITLE     The dispanins: a novel gene family of ancient origin that contains
            14 human members
  JOURNAL   PLoS One 7 (2), e31961 (2012)
   PUBMED   22363774
REFERENCE   6  (bases 1 to 3237)
  AUTHORS   Oort,P.J., Warden,C.H., Baumann,T.K., Knotts,T.A. and Adams,S.H.
  TITLE     Characterization of Tusc5, an adipocyte gene co-expressed in
            peripheral neurons
  JOURNAL   Mol Cell Endocrinol 276 (1-2), 24-35 (2007)
   PUBMED   17689857
  REMARK    GeneRIF: These findings may point to participation of Tusc5 in
            shared adipose-nervous system functions.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK048522.1, BX000359.7 and AK158395.1.
            
            On Sep 29, 2009 this sequence version replaced NM_177709.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK048522.1, AK158395.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2072              AK048522.1         1-2072
            2073-2103           BX000359.7         42402-42432
            2104-3233           AK048522.1         2102-3231
            3234-3237           AK158395.1         3195-3198
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 45.98 cM"
     gene            1..3237
                     /gene="Trarg1"
                     /gene_synonym="C130069F04Rik; DSPB1; Lost1; Tusc5"
                     /note="trafficking regulator of GLUT4 (SLC2A4) 1"
                     /db_xref="GeneID:237858"
                     /db_xref="MGI:MGI:3029307"
     exon            1..665
                     /gene="Trarg1"
                     /gene_synonym="C130069F04Rik; DSPB1; Lost1; Tusc5"
                     /inference="alignment:Splign:2.1.0"
     CDS             288..809
                     /gene="Trarg1"
                     /gene_synonym="C130069F04Rik; DSPB1; Lost1; Tusc5"
                     /note="tumor suppressor candidate 5 homolog; dispanin
                     subfamily B member 1"
                     /codon_start=1
                     /product="trafficking regulator of GLUT4 1"
                     /protein_id="NP_808377.1"
                     /db_xref="CCDS:CCDS25069.1"
                     /db_xref="GeneID:237858"
                     /db_xref="MGI:MGI:3029307"
                     /translation="MANPAQPPLQDPGSTSPLELPEMEKLLTKVENKDDQALNLSKSL
                     SGALDLEQNGHSLPFKVISEGHRQPSLSGSPSRVSSRRASSVITTSYAQDQEAPKDYL
                     VLAIASCFCPVWPLNLIPLIFSIMSRSSVQQGDLDGARRLGRLARLLSITFIILGIVI
                     IIVAVTVNFTVPK"
     exon            666..798
                     /gene="Trarg1"
                     /gene_synonym="C130069F04Rik; DSPB1; Lost1; Tusc5"
                     /inference="alignment:Splign:2.1.0"
     exon            799..3237
                     /gene="Trarg1"
                     /gene_synonym="C130069F04Rik; DSPB1; Lost1; Tusc5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3216..3221
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trarg1"
                     /gene_synonym="C130069F04Rik; DSPB1; Lost1; Tusc5"
                     /note="hexamer: AATAAA"
     polyA_site      3237
                     /gene="Trarg1"
                     /gene_synonym="C130069F04Rik; DSPB1; Lost1; Tusc5"
                     /note="major polyA site"
ORIGIN      
        1 aaagttagcc tcaaacttga acgggagcgg aaacccccag ttccctggga gctccgcggt
       61 ggtggtggct gcagcagcag cagcagcagc agtccagctg tggacgcttc gattcttgag
      121 aagctcttct gaatcttcca acactcagct agcccccagc tctctgcaaa atacatctac
      181 cctcgtcctg gaggtttgaa atctggactt ggggaatggt gtgctgagga ccctctggaa
      241 ctcactatcc ctacctggac ttgaagaaac ccaaagccag agtcaacatg gccaacccag
      301 cgcagcctcc gcttcaggac ccaggctcca cctcaccctt ggaactgcct gagatggaga
      361 aactactcac aaaagtggag aacaaggatg accaagccct gaatctgtcc aagtctctgt
      421 caggggctct ggacctggag cagaatggtc acagcctgcc cttcaaggtc atctccgagg
      481 ggcaccgcca gccctccctt tccggctccc cttcccgggt cagctctcgg cgagcatcct
      541 ccgtcatcac cacatcctac gcccaggacc aagaagcccc caaagattac ctggtccttg
      601 ccattgcctc ttgcttctgc cccgtctggc ccctcaacct catccccctt atcttttcta
      661 tcatgtctcg aagtagtgtg cagcaggggg acctggatgg ggcacggagg ctgggccgcc
      721 tggcacggct actcagcatc accttcatca tcctgggcat cgttatcatc attgtggctg
      781 tcactgtcaa tttcacagtt ccgaagtaac cgcggagctg tttctagcag aggcagaggc
      841 agatgccggc cagctggaga tctccgaacc tacatgtccc cacacattac aaaggacagg
      901 atggtgggtg ctgagtgccc acctgtccct aagatccaaa agcacaaact tggagggaaa
      961 tctctccatc actccattgc cagcccacgg cagacgggag ggaaaatctg aaatgaagtt
     1021 ggctgtggac tcaatctcag agtcacatct ctcgggcttg tttctcatcc cacaattcct
     1081 gggacttaca cgtagcgtaa actgccagaa tgggaagaaa atcagcccca tagccaagaa
     1141 agaagcactg ggtatctatg aacgtgagca catcctggcc gcccgtcttt tctattttta
     1201 ttgaggggga gtaaagcgac tgcttgcatg ctttgtgccg agcatccatg gtgatcccag
     1261 ccacagccca aggtgaatct gtcactcagc acaggccctg gatacccact ggcaaagagg
     1321 caacggccat acggaaatcc tccattgatg actgactgaa tagggatagt atgacagacc
     1381 tggtgctctt gctgaaggtc acgctggagg cttctctacc aaggactctg gatccagagt
     1441 atcttccaga accacaggat ctgcctcttg attttcacct gtgatgtagc ctgtggacac
     1501 caacctggaa gccctagaac ttgacctgtc ccaggtcagc catgggggag ggacagggct
     1561 tgatctctct ctcttgccac ggactctttg gaggtctggg accccgtctt ggtaacccac
     1621 acaagtggct caggagccct atacagggtt ttcaggggac cctacacaga agagctgaag
     1681 tagagcctgg cttcagactg ttagccgagc tctaatggat gctctgtgcc tggtcctgca
     1741 tccctggcca tccacagcaa caaaggcttt ccctagaggt agctttgaga ctattggact
     1801 ctcttgttta ttcataggct ctggggagct gctgcagcat gtaagggagg gggctagaga
     1861 attcttagga aggtttgtta tttcttgatc tggagtccaa ggaggggctc acctgaaagg
     1921 gcagtctggg gagtggggac caggacagga agcaaaggcc cagtagccat agcctctcct
     1981 gagtagaaca cggaaaagaa gctaatctct gccaaacagc ctcatgcttg ttgcatctct
     2041 atttctccac tttttttttt tttttttttt tttttggcct ggcacaggct gtgcccacgg
     2101 gggcttcacc tgcttgcctt gcctgctctg agctccatct acccaatgtc atactccacc
     2161 accaccaact tcagctcaga cctggcagaa gggcttccct tggccagcag ccagcgtttt
     2221 gggggggggg gggaaacagg tccagggctg tgactacact ggtcgggaat tggaagttca
     2281 cttcctctga gcaaaattag gatcctgatc cctcctccct tgggccccat gcttggctcc
     2341 ctgcgatctc agagaggctg ggcagggcac gctagcacag agcactggtc aatgggagag
     2401 agaaggttgg tacccccaaa tcaggccctt attgaggaag tgaacttgga ctcatgttcc
     2461 taaagcaggg gctggctgca caccctttat gggatctttg aaaccaaact agagatgtcc
     2521 tcgttttctg tgggaagtaa gcatgcgagc ccagaatacc tatgtgactt gagaggttgg
     2581 gtcaggagag atgtgctatc taaagaacag cttatcataa actccagatc ctgagtgggt
     2641 ggcctcctaa aggccctccc aggacagata gtgtctaggg attgtcatga tggtttctac
     2701 atcaccagag tccaggtcac ccgcagccat ttcttctcat ctcctagtca cagcctgcca
     2761 gtcacacctt cacccttgca caccctcaca cactcacaca ttgattcaca cttcagcctg
     2821 gaacacgcag ggtctctaca ttagggaatg gcttagctag aacattcttc tacccaggca
     2881 agaacaacct aggagccttc ccgtggttcc agggacacag cctagaggtc cccaagatct
     2941 cccctgagca gaggggagta cagggcagga ggagagagac attagggtgg gaagagagtg
     3001 agcaagagtt ctctcagccc ccagttccca ctgaagctag gagggatggg ggccctgccg
     3061 tcccagctct ggcttggttc agctgccagc tgtatgagca cagtggctga gcggagtgac
     3121 tctttggcct ctctttggcc atcctggtga acagcaagaa ctgccccctc cccaccacac
     3181 gcactagtga ctctgtattt cttggcatgg aaagaaataa agctgaatat atatgtt
//
