LOCUS       NM_028173               2943 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus translocating chain-associating membrane protein 1
            (Tram1), mRNA.
ACCESSION   NM_028173
VERSION     NM_028173.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Wang,H., Liu,C., Han,M., Cheng,C. and Zhang,D.
  TITLE     TRAM1 Promotes Microglia M1 Polarization
  JOURNAL   J Mol Neurosci 58 (2), 287-296 (2016)
   PUBMED   26563450
  REMARK    GeneRIF: TRAM1 plays an essential role in the M1 polarization of
            microglia.  TRAM1 increases M1-related gene expression.
REFERENCE   3  (bases 1 to 2943)
  AUTHORS   Tamborero,S., Vilar,M., Martinez-Gil,L., Johnson,A.E. and
            Mingarro,I.
  TITLE     Membrane insertion and topology of the translocating
            chain-associating membrane protein (TRAM)
  JOURNAL   J Mol Biol 406 (4), 571-582 (2011)
   PUBMED   21237175
  REMARK    GeneRIF: These data indicate that TRAM is recognized by the signal
            recognition particle and translocon components, and suggest a
            membrane topology with eight transmembrane segments, including
            several poorly hydrophobic segments.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC157769.3.
            
            On Dec 19, 2014 this sequence version replaced NM_028173.4.
            
            Summary: This gene encodes a multi-pass transmembrane protein of
            the endoplasmic reticulum (ER). It is involved in the process of
            cotranslational translocation of secretory proteins into the ER.
            [provided by RefSeq, Dec 2014].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK152338.1, BC012401.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164140
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AC157769.3         97205-97241         c
            38-311              AC157769.3         96931-97204         c
            312-375             AC157769.3         88354-88417         c
            376-497             AC157769.3         87021-87142         c
            498-614             AC157769.3         86781-86897         c
            615-673             AC157769.3         85431-85489         c
            674-758             AC157769.3         83699-83783         c
            759-829             AC157769.3         79335-79405         c
            830-934             AC157769.3         79134-79238         c
            935-1078            AC157769.3         77270-77413         c
            1079-1239           AC157769.3         76802-76962         c
            1240-2855           AC157769.3         72112-73727         c
            2856-2943           AC157769.3         72024-72111         c
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 4.18 cM"
     gene            1..2943
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /note="translocating chain-associating membrane protein 1"
                     /db_xref="GeneID:72265"
                     /db_xref="MGI:MGI:1919515"
     exon            1..311
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     CDS             189..1313
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /codon_start=1
                     /product="translocating chain-associated membrane protein
                     1"
                     /protein_id="NP_082449.1"
                     /db_xref="CCDS:CCDS14822.1"
                     /db_xref="GeneID:72265"
                     /db_xref="MGI:MGI:1919515"
                     /translation="MAIRKKSNKNPPLLSHEFLLQNHADIVSCLAMLFLLGLMFEVTA
                     KGAIIFVALQYNVTRPATEEQATESASLYHYGIKDLATVLFYMLVAIIIHAIIQEYVL
                     DKINRRMHFSKTKHSKFNESGQLSAFYLFACVWGTFILISENYISDPTILWRAYPHNL
                     MTFQTKFFYISQLAYWLHAFPELYFQKTKKEDIPRQLVYIGLYLFHIAGAYLLNLNHL
                     GLVLLVLHYFVEFLFHISRLFYFSDEKYQKGFSLWAVLFVLGRLLTLILSVLTVGFGL
                     ARAENQKLDFSTGNFNVLAVRIAVLASICITQAFMMWKFINFQLRRWREHSAFQAPPV
                     KRKPAVTKGRSSRKGTENGVNGTVTSNGADSPRNRKEKSS"
     exon            312..375
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            376..497
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            498..614
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            615..673
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            674..758
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            759..829
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            830..934
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1078
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1239
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..2943
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2836..2841
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /note="hexamer: AATAAA"
     polyA_site      2856
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /note="major polyA site"
     regulatory      2919..2924
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
                     /note="hexamer: ATTAAA"
     polyA_site      2938
                     /gene="Tram1"
                     /gene_synonym="1810049E02Rik; TRAMP"
ORIGIN      
        1 tttttttttt tttctcgggc cgccccggcg gctgcgtact ggctgtggga tgggaagtga
       61 agccccagcg agcggctgcg gcggggctgt gcggagcagc cagcgggagg cggcggcgcg
      121 gcggtgagca gccgagagct cggagcgggg cggaggaagc acctgcaggc acaggcggcg
      181 gctccaccat ggcgattcgc aagaagagca acaagaaccc gccgctgctg agccacgaat
      241 tcttgctgca gaatcacgcg gacatcgtct cctgcctggc gatgcttttc ctgctggggc
      301 ttatgttcga ggtaacagca aaaggagcca tcatctttgt tgctcttcag tataatgtta
      361 cccgccctgc aacagaagaa caagctactg aatcagcatc cctatatcac tatggtatca
      421 aagacttggc tacagttttg ttctacatgc tagtggcgat aattattcat gccataattc
      481 aggagtatgt gttggataaa attaaccgac ggatgcactt ctccaaaacg aagcacagca
      541 agtttaacga gtctggtcag ctcagtgcat tctacctttt tgcctgtgtg tggggcacgt
      601 tcattctcat ctccgaaaac tatatctcag acccaactat cctgtggaga gcctatcccc
      661 ataacctgat gacatttcag acgaagtttt tctacatctc ccagctggcg tactggctcc
      721 atgcattccc tgaactctac ttccagaaaa ccaaaaaaga agatatccct cgtcagctcg
      781 tctacattgg cctttatctc tttcatattg ctggagccta tcttctgaac ttgaaccacc
      841 tgggccttgt ccttctggtg ctgcactatt tcgttgaatt tcttttccac atttctcgtc
      901 tattttattt tagtgacgag aagtatcaga aagggttttc tctctgggca gttctttttg
      961 ttttgggaag acttctgact ctaattcttt cagttcttac tgttggtttt ggtctcgcaa
     1021 gagcggagaa tcagaagctg gactttagta ctggaaactt caacgtgttg gccgttagga
     1081 tcgctgttct cgcgtccatt tgcatcacac aagccttcat gatgtggaag ttcattaact
     1141 tccagcttcg gaggtggagg gaacattctg ccttccaggc cccacctgtg aaaaggaaac
     1201 cggccgtgac caaaggcagg tcttccagaa aaggaacaga gaacggtgtg aacggcacag
     1261 tcacatcaaa cggagcagac tcccctcgga acaggaaaga gaagtcttcg taatggatcg
     1321 gaagctgact gattattgtc cccaaagaat ctgctttctg ctctatcttt cagcactagc
     1381 aatttttctt ttctaaaaat acagtttatg ctctttgatt tctgctgttg tcctgttcct
     1441 gcatcgtaaa gggcattgag gggaggataa tcactctgag tgagaacatt gtagcttaga
     1501 ctcagctacc tgccttcaga atagtttagg gaccaccttt cttttctctt cttttctcct
     1561 cttttctacc cttttctttt tttttttctt tttctttttg ggttgggttt ggtttggttg
     1621 gttttgtttt gttttgtttt gctttgcttt ttgtccttaa tggttttttt ttttttttcc
     1681 taatggtttg gggagaaacc atttacaaac ccctgcataa ccagtgaaca tttttcttgc
     1741 tctcaccaat ttttttataa tattatagca aggtgacctg ttctaagaag gtcatatttt
     1801 ttaagttgtc ttcagggtaa aatggaaata ctataaagtc agatgtcaaa cttttaatgt
     1861 tttcagtgtt ctctaatttt taggaatttt ttgtagcctt tacacctgaa aaaaaaattt
     1921 gtaaaaatca ccaaaatact tgtgtgcttt attttctaag ttgtggctgt acatgttaca
     1981 tcccattcaa aagtactgat ggctactgca agatttattg ccatccattg gagtaggtgt
     2041 cttattaaca taaggaagtg aagtacttag gaagtgaagt gaactttttg tgcattttca
     2101 acatttatca actggcaatc agaaaatgtt tattgtgaag aggaatattc aacttacata
     2161 gccctttttg atatgttaag taatgattct taatgggctt gttgtaagtt tgatatgcat
     2221 agcatcttaa tgagattcta tttaacctgc aagccctttt gaaaagtaat gcctacttga
     2281 tttctattaa aaagaaaaag gtaccaggta attctatttg ggaaacgaat gcactaactt
     2341 taaagaaatt gaaaatccag gtggatagtc ttaacagagg catgctttat gttatgttag
     2401 agctttggac ccatctttaa ttgagaaacg tttatctgta taaaacatat ttttggataa
     2461 atatatatat atatatttgt atctacagaa aggctctgaa aagcatttga gtaaaatctt
     2521 ggtccccttt tctatgcttg tcctcgcacc ctgcgcttgg tctgtccact gttgttcagc
     2581 acatacatgc tccatcgttt ctcactctgt caccagctaa aaaatgtccg tgccatgttg
     2641 actatagaag ccacaagcct caggcctgtg gttaagttgc catgctgcta gaaagtcatg
     2701 ctttctcttc tccagccgct tacctgcttc ctgggaaagt agtcgctccc tggagcagta
     2761 ccgactttca gtggaaccaa atttccgccc ttaagactgg cattatattg aacgatacat
     2821 tgagaaatca atcgaaataa aaaattttta ctttcacaag ctgtttcctg aacaattcct
     2881 tcactgtaat gtttaagcta ttttgaacgt atattctcat taaagtgtac tattaaaaga
     2941 tga
//
