LOCUS       NM_001141977            4365 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus TPX2, microtubule-associated (Tpx2), transcript
            variant 1, mRNA.
ACCESSION   NM_001141977
VERSION     NM_001141977.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Zhou,B., Feng,C., Sun,S., Chen,X., Zhuansun,D., Wang,D., Yu,X.,
            Meng,X., Xiao,J., Wu,L., Wang,J., Wang,J., Chen,K., Li,Z., You,J.,
            Mao,H., Yang,S., Zhang,J., Jiao,C., Li,Z., Yu,D., Wu,X., Zhu,T.,
            Yang,J., Xiang,L., Liu,J., Chai,T., Shen,J., Mao,C.X., Hu,J.,
            Hao,X., Xiong,B., Zheng,S., Liu,Z. and Feng,J.
  TITLE     Identification of signaling pathways that specify a subset of
            migrating enteric neural crest cells at the wavefront in mouse
            embryos
  JOURNAL   Dev Cell 59 (13), 1689-1706 (2024)
   PUBMED   38636517
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4365)
  AUTHORS   Li,L., Sun,Y., Davis,A.E., Shah,S.H., Hamed,L.K., Wu,M.R.,
            Lin,C.H., Ding,J.B. and Wang,S.
  TITLE     Mettl14-mediated m6A modification ensures the cell-cycle
            progression of late-born retinal progenitor cells
  JOURNAL   Cell Rep 42 (6), 112596 (2023)
   PUBMED   37269288
REFERENCE   4  (bases 1 to 4365)
  AUTHORS   Lei,Z., Liu,W., Nie,Y., Yang,Y., Chen,G., Huang,L., Wu,H., Lei,Y.,
            Chen,L., Hu,Q., Rong,H., Yu,S., Song,Q., Tong,F. and Guo,J.
  TITLE     EpCAM Is Essential to Maintaining the Immune Homeostasis of
            Intestines via Keeping the Expression of pIgR in the Intestinal
            Epithelium of Mice
  JOURNAL   Front Immunol 13, 843378 (2022)
   PUBMED   35493520
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4365)
  AUTHORS   Wang,X., Wang,J., Shen,H., Luo,Z. and Lu,X.
  TITLE     Downregulation of TPX2 impairs the antitumor activity of CD8+ T
            cells in hepatocellular carcinoma
  JOURNAL   Cell Death Dis 13 (3), 223 (2022)
   PUBMED   35273149
  REMARK    GeneRIF: Downregulation of TPX2 impairs the antitumor activity of
            CD8+ T cells in hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4365)
  AUTHORS   Mori,D., Yamada,M., Mimori-Kiyosue,Y., Shirai,Y., Suzuki,A.,
            Ohno,S., Saya,H., Wynshaw-Boris,A. and Hirotsune,S.
  TITLE     An essential role of the aPKC-Aurora A-NDEL1 pathway in neurite
            elongation by modulation of microtubule dynamics
  JOURNAL   Nat Cell Biol 11 (9), 1057-1068 (2009)
   PUBMED   19668197
REFERENCE   7  (bases 1 to 4365)
  AUTHORS   Zheng,P. and Dean,J.
  TITLE     Role of Filia, a maternal effect gene, in maintaining euploidy
            during cleavage-stage mouse embryogenesis
  JOURNAL   Proc Natl Acad Sci U S A 106 (18), 7473-7478 (2009)
   PUBMED   19376971
REFERENCE   8  (bases 1 to 4365)
  AUTHORS   Bird,A.W. and Hyman,A.A.
  TITLE     Building a spindle of the correct length in human cells requires
            the interaction between TPX2 and Aurora A
  JOURNAL   J Cell Biol 182 (2), 289-300 (2008)
   PUBMED   18663142
REFERENCE   9  (bases 1 to 4365)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 4365)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ163921.1, CA558942.1, BC060619.1 and AK167030.1.
            
            [WARNING] On Oct 22, 2024 this sequence was replaced by
            NM_001141977.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AK153957.1, AK090101.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN00849380, SAMN00849383 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-140               CJ163921.1         3-142
            141-511             CA558942.1         1-371
            512-989             BC060619.1         400-877
            990-990             AK167030.1         442-442
            991-3062            BC060619.1         879-2950
            3063-4365           AK167030.1         2498-3800
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 75.41 cM"
     gene            1..4365
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /note="TPX2, microtubule-associated"
                     /db_xref="GeneID:72119"
                     /db_xref="MGI:MGI:1919369"
     exon            1..334
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            335..429
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            430..603
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     CDS             498..2735
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /note="TPX2, microtubule-associated protein homolog"
                     /codon_start=1
                     /product="targeting protein for Xklp2"
                     /protein_id="NP_001135449.1"
                     /db_xref="CCDS:CCDS16900.1"
                     /db_xref="GeneID:72119"
                     /db_xref="MGI:MGI:1919369"
                     /translation="MSQVPTTYSFDAPTDFINFSSLDAEEDTENIDSWFDEKANLENK
                     FLRQRGIGEPFQGKNSLRKAKLQQGFVTPLKAVDNTYHKETEKENLQKQSIPSNDCSS
                     LDAKRAVSGNTPVQPQRRSIRLSAQKDLEQKEKNHVASVEMKAKRCVAPATDCPPQKR
                     MKVSHKKKLEEEEEGSAPATSRKNERETLEKAKGKHTVPGVPPAREKVLKSTEEQEIE
                     KRLRMQQEVVELRRKNEEFKKLALAGPGQPVKKSTSQVTKTVDFHFLTDERIKQHPKN
                     QEEYKEVNFMSELRKHSSTPARGTRGCTIIKPFNLSKGKKRTFDEAASTYVPIAQQVE
                     AFHKRTPNRYHLRNKKDESLLPSKSVNKIARDPQTPILQTKYRTRAVTCKSTAEQEAE
                     ELEKLQQYKFKARELDPRIFESGPILPKRAPVKPPTQPVGFDLEIEKRIHERESKKKT
                     EDEQFEFHSRPCPTKILEDVVGVPEKKVIPATVPKSPVFALKNRIRVPIKDEEEEKPV
                     VIKAQPVPHYGVPYKPHIAEARNVEVCPFSFDTRDKERQLQKEKKIKEMQKGEVPKFK
                     ALPVPHFDTINLPEKKVKNVTQAEPFSLETDKRGAYKAEMWKHQLEEEQKQQKDAACF
                     KARPNTVIFQEPFVPKKEKKSLAENPSGSLVQEPFQLATERRAKERQELEKKMAEVEA
                     WKLQQLEEVRQQEEEQQKEELARLRKELVHKANPIRKYAAVEVKSSELPLTVPVSPKF
                     STRFQ"
     exon            604..726
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            727..853
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            854..985
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1111
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1233
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1385
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1554
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1693
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            1694..1910
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..2003
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2180
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            2181..2327
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            2328..2439
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            2440..2627
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            2628..4365
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3042..3047
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /note="hexamer: AATAAA"
     polyA_site      3063
                     /gene="Tpx2"
                     /gene_synonym="2610005B21Rik; DIL2; p100; REPP86"
                     /note="major polyA site"
ORIGIN      
        1 tttgttggct tccggctctc tgattggtgc gttcgaccgg gtgtctgagt tcaaattttc
       61 aaccgtacca gttgagtcct gacgcggtcg tgagtcgctg gactcgtagg gttggtgcaa
      121 gctgaaaccg ggaaagtagc gtgaaggtga ggggaggcag cggtgcgccg gaggccgttt
      181 ttccagggtt ctgagagggc aggggagtcc cccgggcggg tccggacagc ctagacagcg
      241 cgcgggcttt ggacgccggt gacaggactt cggggcgttg gtatttctgg gaaccccgga
      301 gcagaggctg ctccaggctg tcagccacta gacggttctt catgagaagt gtctgccaga
      361 ctgcaagaat tcaagagact tgagaacttg attgtggctt tgagcgaggt tgtcaggtgt
      421 gtatacatga agacagtcta ttgaggagag tattagaatc ctggaaaaag cttgtttttc
      481 tgcactaagt tggggcaatg tcacaagtcc ctactactta ctctttcgat gcccccaccg
      541 actttatcaa tttttcatct ttggatgctg aagaggatac tgaaaatata gactcatggt
      601 ttgatgagaa ggccaacttg gagaacaagt ttcttcgaca gaggggaata ggcgagcctt
      661 ttcaggggaa gaattccttg agaaaagcca aacttcaaca gggcttcgtc acaccgttga
      721 aggcagttga caacacttac cacaaagaga cagaaaagga aaatcttcag aaacagtcta
      781 tcccatcaaa tgattgttct tccctggatg ctaagagagc tgtatcagga aatactcctg
      841 tccagcctca gagaagatct attagactct ctgctcagaa ggatttggag cagaaagaga
      901 aaaaccatgt tgcctctgtt gaaatgaaag ccaagagatg tgttgctccg gccactgatt
      961 gtccccccca gaaaagaatg aaagtttctc ataaaaagaa actggaggaa gaggaggaag
     1021 gcagtgctcc agctacttca agaaagaatg aaagagaaac cctggagaaa gccaagggca
     1081 agcacactgt gccaggtgtg ccacctgcaa gggagaaggt tctaaagagt actgaggagc
     1141 aggagataga gaaaaggctg cggatgcagc aggaggtggt ggagctgcgc aggaaaaacg
     1201 aagagttcaa gaagctcgcg ctcgcagggc cagggcaacc tgtgaagaag tccacgagcc
     1261 aggttaccaa gacagttgac tttcacttcc tcacagatga gcgaatcaag caacatccca
     1321 agaaccagga agagtataag gaagtgaact tcatgtctga acttcggaag cattcttcca
     1381 cgcctgcccg aggaaccaga ggatgcacta tcattaagcc tttcaacctg tccaaaggga
     1441 agaaaagaac atttgatgaa gcagcttcta cgtatgtgcc cattgcacag caggttgaag
     1501 ccttccacaa acgaaccccc aatagatacc atctgaggaa caagaaggac gagagcttgt
     1561 taccctccaa atctgtgaac aagattgcac gagaccccca gacccccata ctgcagacca
     1621 aatatcgtac aagggctgtg acttgcaaaa gtactgcaga gcaggaggcc gaggagcttg
     1681 agaaactgca acaatacaaa ttcaaagcac gggaacttga tcctagaatt tttgaaagtg
     1741 gccccatctt gcccaagaga gcacctgtta aacctcctac tcagcctgtt ggttttgatt
     1801 tggaaattga gaaacgaatt catgagcgag agtcaaagaa aaaaacagaa gatgaacaat
     1861 ttgaatttca ttctagacct tgtcctacta agatcttgga agatgtcgtg ggtgttcctg
     1921 aaaagaaggt aattccagct actgtcccga agtcgccagt ttttgcattg aagaacagga
     1981 tccgagtgcc catcaaagat gaggaagagg agaaaccagt agtgataaaa gctcaacctg
     2041 tgccacatta tggggtgcct tataagcccc acatcgcaga ggcaagaaat gtggaggtgt
     2101 gcccgttctc ctttgatacc cgggacaaag aacgccagtt gcagaaggag aaaaaaataa
     2161 aagagatgca gaaaggggag gtgcccaagt tcaaggcact tcctgtgccc cattttgaca
     2221 ccattaacct gccagagaaa aaggtaaaga acgtgactca agctgagcct ttctccctgg
     2281 agacagacaa gagaggcgcc tacaaggctg agatgtggaa gcaccagctg gaggaagagc
     2341 agaagcagca gaaggacgcc gcttgcttca aggctcgtcc aaacaccgtc atcttccaag
     2401 agcccttcgt tcccaagaag gagaaaaaat cactggctga gaacccttct ggttctctag
     2461 ttcaggaacc ttttcagctg gccaccgaga ggagagccaa agagcggcag gagctggaga
     2521 agaaaatggc tgaagtagag gcctggaaac tgcagcagtt ggaggaagtc aggcagcagg
     2581 aagaggagca gcagaaggag gagttagcca ggctccggaa agaactggtg cacaaggcaa
     2641 atccaatacg gaagtacgca gcagtggagg tgaaatctag cgagctgcct ctgactgtgc
     2701 cggtgtctcc taagttctcc actcggttcc agtagaagct gcatctatat cacaagcatg
     2761 gagcacgcgt cttcagactc acccgccatg tgtggaaaca cacctgataa ctaatggatg
     2821 ccgggtgttt tgaagatgac tctacacagc aactttgaag ctaatgagcc tccactcatt
     2881 tagactttaa ccagacttct tgtagttatt tccttcagac attagccggc cctttcctgt
     2941 tggtcttaac gctggctagg atttcaagaa ttcgttttgg catagtctac ttttcaccta
     3001 atcccccctt attacccctt atatatgtat tttaagcata aaataaaggc agtcaattcc
     3061 tgggttgtgg tttcagtcac agtgtaagtg agaagcagcc agtctattgg attccttctc
     3121 tgttcaaaat gcgtttataa aggaaccctg tgctgcgagg taggaaggga acctctgtaa
     3181 tggctccaag gaagactgcg cagactccgg aagaccttac agagctgctg ttgtagcatg
     3241 ttctttattg ttggtgctga caatgggatg ccacagccta tagtttccaa cctcacttgg
     3301 ataacttttt ttttaaggtc acaagaattt atttaagctc actgttttct ggaatccagt
     3361 ccatcctgga gggggtggtg agatgatact gttcagactg tgtgacagat tggcaccttc
     3421 aaagacctct ctccctgttg gcttacatac tccagttgag ccttgcaggc aaaagtccta
     3481 caacttccca aggcagtgcc aggctatgga ccaagaaaca ctagcctatg gtgggcattt
     3541 tatacctacg tgatagcatt ccacctgtgg gtgctatagg ctcacggtta ttgcccagtg
     3601 caaaggcatg tatttcaact taagaagaat ctgtaatgta attttctttc tgagatttct
     3661 tgattttata tatttggttt ttagaggcga ggtttctatg tagctcttgt tgtcctagaa
     3721 cgtgatttgt agaccagagt tgaccttgaa tgaatttttt ttttttacct gcatgtatgc
     3781 atgtggaatt cttacctacc ctgtgccagt ggatgtctgt ggagtgtgtc agatgccctg
     3841 ggattggagt tacagatggt tgtattggga aaccctgctc ataatgtcag cttttgacat
     3901 taccagaaag cctagactga ggtcggtgca aaatggtgga ttcctttcgt cagcaaggtt
     3961 tccttttctc tacctgaggg atcccggacc acacacctgt ggaatatcac atagccttgg
     4021 ttagatttca ggtccgactt cttttctact tacacgaacc cgttggagta gcaccggaga
     4081 ctgctggatg tcggagtgcc tctgtgtgtg tgatggtttg tatattcttg gaccagggag
     4141 aggcaccatt tggaggtgtg gccttgtttg aataggtgtg gcctggttgg agtaggtgtg
     4201 tcactgtagg ttgacttcct agttgcctgg aagtcagtct tccactagta gcctttggat
     4261 gaagatgtag aactctcagc tctgcctgga tactgccctg ttcccacctt gataatggac
     4321 tgaacctctg agcctgtaag ccagccccaa ttaaatgttg tttat
//
