LOCUS       NM_176842               2133 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus Tp53rk binding protein (Tprkb), transcript variant 2,
            mRNA.
ACCESSION   NM_176842
VERSION     NM_176842.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Iwata,S., Sasaki,T., Nagahara,M. and Iwamoto,T.
  TITLE     An efficient i-GONAD method for creating and maintaining lethal
            mutant mice using an inversion balancer identified from the
            C3H/HeJJcl strain
  JOURNAL   G3 (Bethesda) 11 (8) (2021)
   PUBMED   34849815
REFERENCE   3  (bases 1 to 2133)
  AUTHORS   Braun,D.A., Rao,J., Mollet,G., Schapiro,D., Daugeron,M.C., Tan,W.,
            Gribouval,O., Boyer,O., Revy,P., Jobst-Schwan,T., Schmidt,J.M.,
            Lawson,J.A., Schanze,D., Ashraf,S., Ullmann,J.F.P.,
            Hoogstraten,C.A., Boddaert,N., Collinet,B., Martin,G., Liger,D.,
            Lovric,S., Furlano,M., Guerrera,I.C., Sanchez-Ferras,O., Hu,J.F.,
            Boschat,A.C., Sanquer,S., Menten,B., Vergult,S., De Rocker,N.,
            Airik,M., Hermle,T., Shril,S., Widmeier,E., Gee,H.Y., Choi,W.I.,
            Sadowski,C.E., Pabst,W.L., Warejko,J.K., Daga,A., Basta,T.,
            Matejas,V., Scharmann,K., Kienast,S.D., Behnam,B., Beeson,B.,
            Begtrup,A., Bruce,M., Ch'ng,G.S., Lin,S.P., Chang,J.H., Chen,C.H.,
            Cho,M.T., Gaffney,P.M., Gipson,P.E., Hsu,C.H., Kari,J.A., Ke,Y.Y.,
            Kiraly-Borri,C., Lai,W.M., Lemyre,E., Littlejohn,R.O., Masri,A.,
            Moghtaderi,M., Nakamura,K., Ozaltin,F., Praet,M., Prasad,C.,
            Prytula,A., Roeder,E.R., Rump,P., Schnur,R.E., Shiihara,T.,
            Sinha,M.D., Soliman,N.A., Soulami,K., Sweetser,D.A., Tsai,W.H.,
            Tsai,J.D., Topaloglu,R., Vester,U., Viskochil,D.H.,
            Vatanavicharn,N., Waxler,J.L., Wierenga,K.J., Wolf,M.T.F.,
            Wong,S.N., Leidel,S.A., Truglio,G., Dedon,P.C., Poduri,A., Mane,S.,
            Lifton,R.P., Bouchard,M., Kannu,P., Chitayat,D., Magen,D.,
            Callewaert,B., van Tilbeurgh,H., Zenker,M., Antignac,C. and
            Hildebrandt,F.
  TITLE     Mutations in KEOPS-complex genes cause nephrotic syndrome with
            primary microcephaly
  JOURNAL   Nat Genet 49 (10), 1529-1538 (2017)
   PUBMED   28805828
REFERENCE   4  (bases 1 to 2133)
  AUTHORS   Quina,L.A., Wang,S., Ng,L. and Turner,E.E.
  TITLE     Brn3a and Nurr1 mediate a gene regulatory pathway for habenula
            development
  JOURNAL   J Neurosci 29 (45), 14309-14322 (2009)
   PUBMED   19906978
REFERENCE   5  (bases 1 to 2133)
  AUTHORS   Daigo,Y., Takayama,I., Ward,S.M., Sanders,K.M. and Fujino,M.A.
  TITLE     Isolation of novel mouse genes that were differentially expressed
            in W/W(V) mouse fundus
  JOURNAL   J Gastroenterol 39 (3), 238-241 (2004)
   PUBMED   15065000
REFERENCE   6  (bases 1 to 2133)
  AUTHORS   Miyoshi,A., Kito,K., Aramoto,T., Abe,Y., Kobayashi,N. and Ueda,N.
  TITLE     Identification of CGI-121, a novel PRPK (p53-related protein
            kinase)-binding protein
  JOURNAL   Biochem Biophys Res Commun 303 (2), 399-405 (2003)
   PUBMED   12659830
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY108059.1, BM948212.1 and AC158679.8.
            
            On Dec 19, 2009 this sequence version replaced NM_176842.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 3. Variants 1-5 all encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC024858.1, SRR9219381.80209.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-349               BY108059.1         2-350
            350-990             BM948212.1         87-727
            991-2133            AC158679.8         109072-110214       c
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 37.53 cM"
     gene            1..2133
                     /gene="Tprkb"
                     /gene_synonym="0610033G21Rik; 1810034M08Rik; Cgi-121;
                     DRWMS1; mDRWMS1"
                     /note="Tp53rk binding protein"
                     /db_xref="GeneID:69786"
                     /db_xref="MGI:MGI:1917036"
     exon            1..72
                     /gene="Tprkb"
                     /gene_synonym="0610033G21Rik; 1810034M08Rik; Cgi-121;
                     DRWMS1; mDRWMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            73..161
                     /gene="Tprkb"
                     /gene_synonym="0610033G21Rik; 1810034M08Rik; Cgi-121;
                     DRWMS1; mDRWMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            162..324
                     /gene="Tprkb"
                     /gene_synonym="0610033G21Rik; 1810034M08Rik; Cgi-121;
                     DRWMS1; mDRWMS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             184..711
                     /gene="Tprkb"
                     /gene_synonym="0610033G21Rik; 1810034M08Rik; Cgi-121;
                     DRWMS1; mDRWMS1"
                     /note="isoform a is encoded by transcript variant 2;
                     TP53RK-binding protein; Prpk (p53-related protein
                     kinase)-binding protein; EKC/KEOPS complex subunit Tprkb;
                     PRPK-binding protein"
                     /codon_start=1
                     /product="EKC/KEOPS complex subunit Tprkb isoform a"
                     /protein_id="NP_789812.1"
                     /db_xref="CCDS:CCDS20305.1"
                     /db_xref="GeneID:69786"
                     /db_xref="MGI:MGI:1917036"
                     /translation="MQLSQQLDLFPECRVTLLLFKDVKNAGDLRKKAMEGSIDGSLIN
                     PNVIVDPFQILVAANKAVHLHRLGKMKTRTLSTEIIFNLSPNNNISEALKKFGISETN
                     TSVLIVYIEDGSKQVPQEHLVSQVEGQQVPLESLPEITRLSEVKKIYKLSSQEERIGT
                     LLDAIICRMSTKDVL"
     exon            325..447
                     /gene="Tprkb"
                     /gene_synonym="0610033G21Rik; 1810034M08Rik; Cgi-121;
                     DRWMS1; mDRWMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            448..624
                     /gene="Tprkb"
                     /gene_synonym="0610033G21Rik; 1810034M08Rik; Cgi-121;
                     DRWMS1; mDRWMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            625..2133
                     /gene="Tprkb"
                     /gene_synonym="0610033G21Rik; 1810034M08Rik; Cgi-121;
                     DRWMS1; mDRWMS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2112..2117
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tprkb"
                     /gene_synonym="0610033G21Rik; 1810034M08Rik; Cgi-121;
                     DRWMS1; mDRWMS1"
                     /note="hexamer: ATTAAA"
     polyA_site      2131
                     /gene="Tprkb"
                     /gene_synonym="0610033G21Rik; 1810034M08Rik; Cgi-121;
                     DRWMS1; mDRWMS1"
                     /note="major polyA site"
ORIGIN      
        1 attaagcact tccggaagta gagagctaag tttcctaggg gacgatcttc tgatgcgggc
       61 gtaggatgca gggcattttc ccgactctac gggcccgtgc ttccaatctc ctgtaatcct
      121 aaacttctga ctctcctgac ctcccctgga ctggattctc ggagagtcct tcttgtctac
      181 gaaatgcagc tatcacagca gctggacttg tttcctgaat gcagggtgac ccttctgtta
      241 tttaaagatg taaaaaatgc aggagacttg agaaaaaagg ccatggaagg atctattgat
      301 ggttcattga taaaccccaa tgtgattgtc gatccatttc agatacttgt ggcagcaaac
      361 aaagccgttc atctccacag actgggaaaa atgaagacaa gaactctgtc tactgaaatt
      421 atcttcaacc tctccccaaa taataatatt tcagaggcct tgaagaaatt tggcatttca
      481 gaaactaaca cttcagttct gattgtttac attgaagatg gaagcaaaca agtaccacag
      541 gaacacctag tatctcaagt ggaaggccag caggtgcctt tggaaagtct tccagaaata
      601 acaaggctct cggaagtcaa gaagatatat aaactgtcat cacaagaaga gaggattggg
      661 acgttattgg atgctatcat ttgtagaatg tcaacgaagg atgttttata agatgtttaa
      721 aatgtcaaca gaaagccggg cgtggtgatg catgccttta atcccagcac ttgggaggca
      781 gaggcaggcg gatttctgag tttgaggcca gcctggtcta cagagtgagt tccaggacag
      841 ccagggctac acggagaaac cctgtctcga aaaaccaaaa aaaaaaaaaa attaactcta
      901 agccaggtgt ggtgactcat tacagtccac tgctcaggag gctgaggcag gagaaagctg
      961 caagtttgag gtcagcttgg gatatgccac aaattccaga tcagcctaaa gtactatgtc
     1021 aaatcctatc tcaataaata gcaattcatt taactctctt agctgctttt taggtagata
     1081 tgtctttgtg cccactttat acaagaggca gctaaaagag tatagcagta ttaactgact
     1141 agcccagggt catgtaacta gtataaaacc atgtttgatg gttccttttc cccctcactt
     1201 tatcttgtat tggattgtat ccatcaagat aggcttggtc aagggttagc aaagctctgt
     1261 gaagagccag ataagtactt tgctcctcaa aggccgcctc atctggttcc tgctacaatt
     1321 actcaatatg acctttgcag tctgaaagca gccacagagg acaggtacag agtgagtggc
     1381 ctctgtccag tgagccttga cttgaacaag cagtgggcag ccctggagtt gagactgtag
     1441 actccagagt tggctaggac atagtgacag tcaaacccta aagctcactg gcttagtaca
     1501 gcagaaggaa acattttgtt atcttgaagg cagaagggat cagagggaaa cccacagcag
     1561 ggctttagag ccctctgcgg gggcttctca gcctgggctt ctaggttact gtgcagcttg
     1621 tcagaagctc tggggagggc agagaaacct aaaagcatcc acatggaagt ggcaggtatt
     1681 gctcacctct tttcattcac acaagttcaa aaatggtgac cccttgttct tcccacaagc
     1741 acagtcagtt ctgcccctcc agtcccctct aaagcgccaa gtctgcaaag ccaggtgctc
     1801 tcatagtgca gttctggggt tgttctctgg gtaccttatg gtggtttgaa tatgtttggc
     1861 ccagggagtg gcactattta gagatgaggc ctttttggag ttggtaagac cttggcagaa
     1921 atgtgtcatt gtgggcatgg gctttaagag accctccctc cttctagccg tgtgggagcc
     1981 agttttctct tagcggcctt cagatgaaga tgtagaactc tcagatcctc ctgcaccatg
     2041 cctgcctgga cactgccatg ctcctgtctt gatgataaca gactgaacct ctgaacctgt
     2101 aagccagccc gattaaatgt tgttcttata aga
//
