LOCUS       NM_001310540            4669 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus tripeptidyl peptidase II (Tpp2), transcript variant 2,
            mRNA.
ACCESSION   NM_001310540 XM_006495885
VERSION     NM_001310540.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Zhao,J., He,C., Fan,X., Wang,L., Zhao,L., Liu,H., Shen,W.,
            Jiang,S., Pei,K., Gao,J., Qi,Y., Liu,Y., Zhao,J., Zhang,R., Lu,C.,
            Tong,J. and Huai,J.
  TITLE     Tripeptidyl peptidase II coordinates the homeostasis of calcium and
            lipids in the central nervous system and its depletion causes
            presenile dementia in female mice through calcium/lipid
            dyshomeostasis-induced autophagic degradation of CYP19A1
  JOURNAL   Theranostics 14 (4), 1390-1429 (2024)
   PUBMED   38389851
  REMARK    GeneRIF: Tripeptidyl peptidase II coordinates the homeostasis of
            calcium and lipids in the central nervous system and its depletion
            causes presenile dementia in female mice through calcium/lipid
            dyshomeostasis-induced autophagic degradation of CYP19A1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Stepensky,P., Rensing-Ehl,A., Gather,R., Revel-Vilk,S., Fischer,U.,
            Nabhani,S., Beier,F., Brummendorf,T.H., Fuchs,S., Zenke,S.,
            Firat,E., Pessach,V.M., Borkhardt,A., Rakhmanov,M., Keller,B.,
            Warnatz,K., Eibel,H., Niedermann,G., Elpeleg,O. and Ehl,S.
  TITLE     Early-onset Evans syndrome, immunodeficiency, and premature
            immunosenescence associated with tripeptidyl-peptidase II
            deficiency
  JOURNAL   Blood 125 (5), 753-761 (2015)
   PUBMED   25414442
REFERENCE   3  (bases 1 to 4669)
  AUTHORS   Nahalkova,J. and Tomkinson,B.
  TITLE     TPPII, MYBBP1A and CDK2 form a protein-protein interaction network
  JOURNAL   Arch Biochem Biophys 564, 128-135 (2014)
   PUBMED   25303791
REFERENCE   4  (bases 1 to 4669)
  AUTHORS   Zhou,Y., Ru,Y., Wang,C., Wang,S., Zhou,Z. and Zhang,Y.
  TITLE     Tripeptidyl peptidase II regulates sperm function by modulating
            intracellular Ca(2+) stores via the ryanodine receptor
  JOURNAL   PLoS One 8 (6), e66634 (2013)
   PUBMED   23818952
  REMARK    GeneRIF: Data indicate that TPPII can regulate sperm maturation by
            modulating intracellular Ca(2+) stores via the type 3 RyR.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4669)
  AUTHORS   Eklund,S., Lindas,A.C., Hamnevik,E., Widersten,M. and Tomkinson,B.
  TITLE     Exploring the active site of tripeptidyl-peptidase II through
            studies of pH dependence of reaction kinetics
  JOURNAL   Biochim Biophys Acta 1824 (4), 561-570 (2012)
   PUBMED   22266401
  REMARK    GeneRIF: Previously unknown differences between TPP II orthologues
            and subtilisin as well as features that might be conserved within
            the entire family of subtilisin-like serine peptidases.
REFERENCE   6  (bases 1 to 4669)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 4669)
  AUTHORS   Tomkinson,B., Hansen,M. and Cheung,W.F.
  TITLE     Structure-function studies of recombinant murine
            tripeptidyl-peptidase II: the extra domain which is subject to
            alternative splicing is involved in complex formation
  JOURNAL   FEBS Lett 405 (3), 277-280 (1997)
   PUBMED   9108304
REFERENCE   8  (bases 1 to 4669)
  AUTHORS   Bermingham,N.A., McKay,T., Hoyle,J., Hernandez,D., Martin,J.E. and
            Fisher,E.M.
  TITLE     The gene encoding tripeptidyl peptidase II maps to chromosome 1 in
            the mouse
  JOURNAL   Mamm Genome 7 (5), 390 (1996)
   PUBMED   8661741
REFERENCE   9  (bases 1 to 4669)
  AUTHORS   Tomkinson,B.
  TITLE     Characterization of cDNA for murine tripeptidyl-peptidase II
            reveals alternative splicing
  JOURNAL   Biochem J 304 (Pt 2) (Pt 2), 517-523 (1994)
   PUBMED   7998988
  REMARK    Erratum:[Biochem J. 1995 Mar 15;306 ( Pt 3):880. doi:
            10.1042/bj3060880.. PMID: 7702588]
REFERENCE   10 (bases 1 to 4669)
  AUTHORS   Epstein,D.J., Vekemans,M. and Gros,P.
  TITLE     Splotch (Sp2H), a mutation affecting development of the mouse
            neural tube, shows a deletion within the paired homeodomain of
            Pax-3
  JOURNAL   Cell 67 (4), 767-774 (1991)
   PUBMED   1682057
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY147762.1 and BC060627.1.
            
            On Jul 15, 2015 this sequence version replaced XM_006495885.2.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (2) has the same
            N- and C-termini but is shorter compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC060627.1, AK141908.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 BY147762.1         1-8
            9-504               BC060627.1         1-496
            505-4669            BC060627.1         498-4662
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 23.5 cM"
     gene            1..4669
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /note="tripeptidyl peptidase II"
                     /db_xref="GeneID:22019"
                     /db_xref="MGI:MGI:102724"
     exon            1..260
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..3845
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /EC_number="3.4.14.10"
                     /note="isoform 2 is encoded by transcript variant 2;
                     tripeptidyl-peptidase 2; TPP-II; tripeptidyl
                     aminopeptidase"
                     /codon_start=1
                     /product="tripeptidyl-peptidase 2 isoform 2"
                     /protein_id="NP_001297469.1"
                     /db_xref="CCDS:CCDS78577.1"
                     /db_xref="GeneID:22019"
                     /db_xref="MGI:MGI:102724"
                     /translation="MATAATEEPFPFHGLLPKKETGASSFLCRYPEYDGRGVLIAVLD
                     TGVDPGAPGMQVTTDGKPKIIDIIDTTGSGDVNTATEVEPKDGEIIGLSGRVLKIPAN
                     WTNPLGKYHIGIKNGYDFYPKALKERIQKERKEKIWDPIHRVALAEACRKQEEFDIAN
                     NGSSQANKLIKEELQSQVELLNSFEKKYSDPGPVYDCLVWHDGETWRACVDSNENGDL
                     SKCAVLRNYKEAQEYSSFGTAEMLNYSVNIYDDGNLLSIVTSGGAHGTHVASIAAGHF
                     PEEPERNGVAPGAQILSIKIGDTRLSTMETGTGLIRAMIEVINHKCDLVNYSYGEATH
                     WPNSGRICEVINEAVWKHNTIYVSSAGNNGPCLSTVGCPGGTTSSVIGVGAYVSPDMM
                     VAEYSLREKLPANQYTWSSRGPSADGALGVSISAPGGAIASVPNWTLRGTQLMNGTSM
                     SSPNACGGIALVLSGLKANNVDYTVHSVRRALENTAIKADNIEVFAQGHGIIQVDKAY
                     DYLIQNTSFANRLGFTVTVGNNRGIYLRDPVQVAAPSDHGVGIEPVFPENTENSEKIS
                     FQLHLALTSNSSWVQCPSHLELMNQCRHINIRVDPRGLREGLHYTEVCGYDIASPNAG
                     PLFRVPITAVIAAKVNESSHYDLAFTDVHFKPGQIRRHFVEVPEGATWAEVTVCSCSS
                     EVSAKFVLHAVQLVKQRAYRSHEFYKFCSLPEKGTLIEAFPVLGGKAIEFCIARWWAS
                     LSDVNIDYTISFHGIVCTAPQLNIHASEGINRFDVQSSLKYEDLAPCITLKSWVQTLR
                     PVNAKTRPLGSRDVLPNNRQLYEMVLTYSFHQPKSGEVTPSCPLLCELLYESEFDSQL
                     WIIFDQNKRQMGSGDAYPHQYSLKLEKGDYTIRLQIRHEQISDLDRLKDLPFIVSHRL
                     SNTLSLDIHENHSLALLGKKKSSSLTLPPKYNQPFFVTSLPDDKIPKGAGPGCYLAGS
                     LTLSKTELGKKADVIPVHYYLIPPPTKIKNGSKDKEKDSEKEKDLKEEFTEALRDLKI
                     QWMTKLDSTDIYNELKETYPAYLPLYVARLHQLDAEKERMKRLNEIVDAANAVISHID
                     QTALAVYIAMKTDPRPDAATIKNDMDKQKSTLIDALCRKGCALADHLLHTQPHDGAAA
                     GDAEAKEEEGESTMESLSETYWETTKWTDLFDTKVLIFAYKHALVNKMYGRGLKFATK
                     LVEEKPTKENWKNCIQLMKLLGWTHCASFTENWLPIMYPPDYCVF"
     exon            261..389
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            390..485
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            486..590
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            591..715
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            716..879
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1034
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1111
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1239
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1339
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1488
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1604
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1773
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            1774..1931
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            1932..2008
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2115
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            2116..2270
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            2271..2387
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            2388..2488
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            2489..2585
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            2586..2723
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            2724..2968
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            2969..3047
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            3048..3199
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            3200..3296
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            3297..3427
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            3428..3635
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            3636..3755
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     exon            3756..4652
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3967..3972
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /note="hexamer: ATTAAA"
     polyA_site      3994
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /note="major polyA site"
     regulatory      4630..4635
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /note="hexamer: AATAAA"
     polyA_site      4652
                     /gene="Tpp2"
                     /gene_synonym="TPP-2; TppII"
                     /note="major polyA site"
ORIGIN      
        1 acctccgtcc cgcggtagcc gggtgtcctc gcgccgctcg tccgcgcgct gcctggcagt
       61 ttgcctcttc ctcgtctgtc ctccagcttg cgtccatggc caccgccgcg accgaggagc
      121 cctttccttt ccacggtctt ctaccaaaga aggagaccgg ggcctcctcc ttcctgtgcc
      181 gctacccgga gtatgacggg cgcggggtgc tcatcgccgt cctggacaca ggggtcgatc
      241 ccggggcccc gggcatgcag gttacaactg atggaaaacc aaaaatcatt gatatcattg
      301 atacaacagg aagtggtgat gtaaatactg ctacagaagt agaaccaaaa gatggtgaaa
      361 ttattggtct ttctggaaga gtgcttaaga ttcctgcaaa ctggacaaat cctttaggaa
      421 aatatcatat tggcattaaa aatggttatg acttctatcc aaaggctctc aaggaaagga
      481 tacagaaaga acggaaggaa aaaatctggg atccaattca cagagttgca cttgcagaag
      541 cttgtagaaa acaagaagaa tttgatattg ccaacaatgg ctcttcccaa gccaataaac
      601 taatcaagga agagttgcaa agtcaagtgg aattacttaa ttcttttgag aaaaagtata
      661 gtgatccagg ccctgtatat gactgcttgg tgtggcatga tggtgagacc tggagagcct
      721 gtgttgattc aaatgagaat ggggacttga gtaaatgtgc cgtattgaga aactacaaag
      781 aggcccaaga gtacagttct tttggcacag ctgagatgct gaattactct gtgaacattt
      841 atgacgatgg gaacctgctc tccattgtga ccagcggagg agctcatgga acccatgtag
      901 caagtatagc cgcagggcat tttccagaag agcctgaacg gaatggagtt gctcctggtg
      961 ctcaaattct atccattaag attggtgata cacggctaag cactatggaa acaggcacag
     1021 gcctcatcag agctatgata gaagttataa atcataagtg tgatcttgtc aactacagtt
     1081 atggagaagc aactcattgg ccaaattctg ggagaatttg tgaagtaatt aatgaagcag
     1141 tatggaaaca taatacaatt tatgtttcaa gtgctggaaa taatggtcca tgcctttcta
     1201 cagtgggttg tccaggagga actacatcca gtgtgatagg tgttggagct tatgtttccc
     1261 ctgatatgat ggttgcagag tattcactga gagagaaact gcctgcaaat caatatacat
     1321 ggtcttctag aggcccaagt gctgatggag ccctcggtgt gagcatcagt gcaccaggag
     1381 gtgctattgc ttctgtgcct aactggacat tgagggggac tcagctaatg aatgggacat
     1441 caatgtcttc ccccaatgcc tgtggtggca ttgccctggt actttcaggg ctgaaagcaa
     1501 ataatgttga ctatactgta cactcagtca gaagagctct agaaaatact gcaataaaag
     1561 ctgacaatat agaagtattt gcccaaggac atggaattat tcaggttgac aaagcttatg
     1621 actacctcat tcaaaataca tcatttgcta acagattagg ttttacagtt actgttggaa
     1681 ataaccgtgg tatctacctc cgagatcctg tccaggtggc tgctccttca gatcatggtg
     1741 ttggcattga gcctgtattt ccagagaaca cagaaaactc tgaaaaaata tcctttcagc
     1801 ttcatttagc tttaacttca aattcatctt gggttcagtg tcccagccat ttggaactca
     1861 tgaatcagtg tcggcacata aacatacgtg tggaccccag gggcttaaga gaagggttac
     1921 attatacaga ggtatgtggc tatgatatag catcccccaa tgcaggtcct ttattcagag
     1981 ttccaatcac tgcagttata gcagcaaaag taaatgagtc atcacattat gatctagcct
     2041 ttacagatgt acattttaaa cctggtcaga ttcgaagaca ttttgttgag gttcctgaag
     2101 gggcaacctg ggctgaagtt accgtgtgtt catgttcttc tgaggtatcg gcaaaatttg
     2161 ttcttcatgc agtacagctt gtgaagcaga gagcatatcg aagtcatgaa ttttataagt
     2221 tttgttccct tccagaaaaa ggaacactta ttgaagcttt tcctgttttg ggcggaaaag
     2281 caattgaatt ttgtattgct cgttggtggg caagtcttag tgatgtcaat attgattata
     2341 ccatatcatt ccatgggata gtgtgtactg caccacagtt aaacattcat gcatctgaag
     2401 gaatcaatcg ttttgatgtt cagtcctctt taaagtatga agatctggct ccttgcataa
     2461 ctttgaagag ctgggtgcaa acgctacgcc cagtaaatgc aaaaaccaga cctttaggat
     2521 caagagatgt tttgccaaat aatcgccagc tttatgagat ggtccttaca tacagctttc
     2581 atcagcccaa gagcggagaa gtaacaccta gttgtccact cctttgtgaa ttgttatatg
     2641 agtcagaatt tgacagtcag ttgtggatta ttttcgacca gaacaaaaga cagatgggct
     2701 caggcgatgc ctatccacat cagtattctc tgaaattgga gaaaggagat tatacaattc
     2761 gattacagat tcgtcatgag caaatcagtg atttggatcg tctcaaagat cttccgttta
     2821 ttgtttcaca taggttgtct aataccttga gcttagatat tcatgaaaat catagccttg
     2881 cacttctagg aaagaagaaa tcaagcagct taacattacc acccaaatac aatcagccat
     2941 tctttgttac ttccttacct gatgataaaa tacctaaggg ggcaggaccc ggatgctacc
     3001 ttgcaggctc cttgacattg tcaaagactg agcttggaaa gaaagctgat gtgatccctg
     3061 ttcattacta tctcatacct ccaccaacaa agattaagaa tggcagcaaa gataaagaaa
     3121 aggattcaga aaaagagaaa gacttgaaag aagagtttac tgaagcatta cgcgatctca
     3181 aaattcagtg gatgaccaag cttgattcta ctgacattta caatgaattg aaagaaacat
     3241 atcctgctta ccttcctttg tatgttgcac gtcttcatca actggatgct gaaaaggaac
     3301 gaatgaaaag acttaatgaa attgttgatg ctgccaatgc tgttatttct cacatcgatc
     3361 aaaccgctct tgcagtttac attgcaatga agactgaccc caggcctgat gcagctacta
     3421 taaaaaatga tatggacaag cagaaatcca ccctgataga tgccctctgc aggaaaggat
     3481 gtgccctggc agatcacctt cttcatacac agcctcacga cggggcagca gctggagatg
     3541 ctgaagcaaa ggaagaagaa ggagaaagta ccatggaatc tctatcggaa acctattggg
     3601 aaactacaaa gtggacagat ctttttgaca ctaaggtttt gatatttgca tacaagcatg
     3661 cattagtaaa taagatgtat gggagaggcc ttaagtttgc aaccaaactc gtagaagaaa
     3721 aaccaacaaa agaaaactgg aaaaattgta ttcaactcat gaaattactt ggatggaccc
     3781 actgtgcatc ttttactgaa aactggctcc ccatcatgta tcctcctgat tattgtgtat
     3841 tctaaaataa gaaccaaaac tttacatttc aaaacagaaa attttatagt gaatggatat
     3901 atgaagaatt gtgtggcatt tttagtctga ctaatgcatg ttttcatcta ccattcaata
     3961 ctgatcatta aaactatatg tatttatcag agaactcagt ggcgtgtggc ttaatacatg
     4021 taatacagac ctctgacatc atgctgcttt cctactgcct ccgacactcg gcaagtagag
     4081 agcactccct gcctgccagc cccacctgag ttggatgcag ctgcacactg catgagaggt
     4141 tttcatgttt taattttagc tgtaaaatgt catcctgttt tttattttat aaatcacaaa
     4201 ggttaaaaca tgctaaattt ttccaattat atagaggcct taaaaatgct acattaggtg
     4261 tagctaaatt atttatttga ctaaaaaata tgggaacata atttccagac tttttttcta
     4321 aaaataaatt caattagtgg ggatggtggg gggcataaac aaatggcaac tgggaaaaga
     4381 aacatgttaa ggaaatctcc ataaggagtc ataacaaaaa caaggtgttt ttagagcacc
     4441 ctcaattaca tagcatcaga atgagttaaa atttcttgtg ttggtgacag acagctgaat
     4501 ctatctggtt ttgtaaagaa atacacagta tgtttataac attgaaatca tgtaaaatac
     4561 atgaataaac gtgcaaaacc acaggcacag tacaccatat gcactctgat accttatttt
     4621 ttttttataa ataaaagtaa atattaaagc taaaaaaaaa aaaaaaaaa
//
