LOCUS       NM_001001491            2099 bp    mRNA    linear   ROD 06-JUN-2024
DEFINITION  Mus musculus tropomyosin 4 (Tpm4), mRNA.
ACCESSION   NM_001001491
VERSION     NM_001001491.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Pleines,I., Woods,J., Chappaz,S., Kew,V., Foad,N.,
            Ballester-Beltran,J., Aurbach,K., Lincetto,C., Lane,R.M.,
            Schevzov,G., Alexander,W.S., Hilton,D.J., Astle,W.J., Downes,K.,
            Nurden,P., Westbury,S.K., Mumford,A.D., Obaji,S.G., Collins,P.W.,
            Delerue,F., Ittner,L.M., Bryce,N.S., Holliday,M., Lucas,C.A.,
            Hardeman,E.C., Ouwehand,W.H., Gunning,P.W., Turro,E., Tijssen,M.R.
            and Kile,B.T.
  TITLE     Mutations in tropomyosin 4 underlie a rare form of human
            macrothrombocytopenia
  JOURNAL   J Clin Invest 127 (3), 814-829 (2017)
   PUBMED   28134622
  REMARK    GeneRIF: findings demonstrate a nonredundant role for TPM4 in
            platelet biogenesis in humans and mice and reveal that truncating
            variants in TPM4 cause a previously undescribed dominant Mendelian
            platelet disorder.
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Geeves,M.A., Hitchcock-DeGregori,S.E. and Gunning,P.W.
  TITLE     A systematic nomenclature for mammalian tropomyosin isoforms
  JOURNAL   J Muscle Res Cell Motil 36 (2), 147-153 (2015)
   PUBMED   25369766
  REMARK    Review article
REFERENCE   3  (bases 1 to 2099)
  AUTHORS   Liu,X., Ramjiganesh,T., Chen,Y.H., Chung,S.W., Hall,S.R.,
            Schissel,S.L., Padera,R.F. Jr., Liao,R., Ackerman,K.G.,
            Kajstura,J., Leri,A., Anversa,P., Yet,S.F., Layne,M.D. and
            Perrella,M.A.
  TITLE     Disruption of striated preferentially expressed gene locus leads to
            dilated cardiomyopathy in mice
  JOURNAL   Circulation 119 (2), 261-268 (2009)
   PUBMED   19118250
REFERENCE   4  (bases 1 to 2099)
  AUTHORS   Trimarchi,J.M., Stadler,M.B. and Cepko,C.L.
  TITLE     Individual retinal progenitor cells display extensive heterogeneity
            of gene expression
  JOURNAL   PLoS One 3 (2), e1588 (2008)
   PUBMED   18270576
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2099)
  AUTHORS   McMichael,B.K. and Lee,B.S.
  TITLE     Tropomyosin 4 regulates adhesion structures and resorptive capacity
            in osteoclasts
  JOURNAL   Exp Cell Res 314 (3), 564-573 (2008)
   PUBMED   18036591
  REMARK    GeneRIF: These results indicate that Tm-4 plays a role in
            regulating adhesion structures of osteoclasts, most likely by
            stabilizing the actin microfilaments present in podosomes and the
            sealing zone.
REFERENCE   6  (bases 1 to 2099)
  AUTHORS   Vlahovich,N., Schevzov,G., Nair-Shaliker,V., Ilkovski,B.,
            Artap,S.T., Joya,J.E., Kee,A.J., North,K.N., Gunning,P.W. and
            Hardeman,E.C.
  TITLE     Tropomyosin 4 defines novel filaments in skeletal muscle associated
            with muscle remodelling/regeneration in normal and diseased muscle
  JOURNAL   Cell Motil Cytoskeleton 65 (1), 73-85 (2008)
   PUBMED   17968984
  REMARK    GeneRIF: Tropomyosin 4 defines longitudinal filaments in mouse
            models of muscular dystrophy and nemaline myopathy.
REFERENCE   7  (bases 1 to 2099)
  AUTHORS   McMichael,B.K., Kotadiya,P., Singh,T., Holliday,L.S. and Lee,B.S.
  TITLE     Tropomyosin isoforms localize to distinct microfilament populations
            in osteoclasts
  JOURNAL   Bone 39 (4), 694-705 (2006)
   PUBMED   16765662
  REMARK    GeneRIF: Tm-4 is specifically enriched within and around osteoclast
            attachment structures, the sealing zone and podosomes.
REFERENCE   8  (bases 1 to 2099)
  AUTHORS   Hossain,M.M., Crish,J.F., Eckert,R.L., Lin,J.J. and Jin,J.P.
  TITLE     h2-Calponin is regulated by mechanical tension and modifies the
            function of actin cytoskeleton
  JOURNAL   J Biol Chem 280 (51), 42442-42453 (2005)
   PUBMED   16236705
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY230591.1, BC070421.1 and AC158898.7.
            
            On Jun 16, 2018 this sequence version replaced NM_001001491.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC070421.1, SRR3224856.966.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 BY230591.1         2-5
            5-2086              BC070421.1         1-2082
            2087-2099           AC158898.7         106825-106837       c
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 34.81 cM"
     gene            1..2099
                     /gene="Tpm4"
                     /gene_synonym="2610528G24Rik; Tpm4.2; Tpm4.2cy"
                     /note="tropomyosin 4"
                     /db_xref="GeneID:326618"
                     /db_xref="MGI:MGI:2449202"
     exon            1..166
                     /gene="Tpm4"
                     /gene_synonym="2610528G24Rik; Tpm4.2; Tpm4.2cy"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..781
                     /gene="Tpm4"
                     /gene_synonym="2610528G24Rik; Tpm4.2; Tpm4.2cy"
                     /codon_start=1
                     /product="tropomyosin alpha-4 chain"
                     /protein_id="NP_001001491.1"
                     /db_xref="CCDS:CCDS22408.1"
                     /db_xref="GeneID:326618"
                     /db_xref="MGI:MGI:2449202"
                     /translation="MAGLNSLEAVKRKIQALQQQADDAEDRAQGLQRELDGERERREK
                     AEGDAAALNRRIQLLEEELDRAQEQLATALQNLEEAEKAADESERGMKVIENRAMKDE
                     EKMEILEMQLKEAKHITDEADRKYEEVARKLVILEGELKRAEERAEVSELKCGDLEEE
                     LKNVTNNLKSLEAASEKYSEKEDKYEEEIKLLSDKLKEAETRAEFAERTVSKLEKTID
                     DLEEKLAQAKEENVGLHQTLDQTLNELNCI"
     exon            167..300
                     /gene="Tpm4"
                     /gene_synonym="2610528G24Rik; Tpm4.2; Tpm4.2cy"
                     /inference="alignment:Splign:2.1.0"
     exon            301..418
                     /gene="Tpm4"
                     /gene_synonym="2610528G24Rik; Tpm4.2; Tpm4.2cy"
                     /inference="alignment:Splign:2.1.0"
     exon            419..489
                     /gene="Tpm4"
                     /gene_synonym="2610528G24Rik; Tpm4.2; Tpm4.2cy"
                     /inference="alignment:Splign:2.1.0"
     exon            490..565
                     /gene="Tpm4"
                     /gene_synonym="2610528G24Rik; Tpm4.2; Tpm4.2cy"
                     /inference="alignment:Splign:2.1.0"
     exon            566..628
                     /gene="Tpm4"
                     /gene_synonym="2610528G24Rik; Tpm4.2; Tpm4.2cy"
                     /inference="alignment:Splign:2.1.0"
     exon            629..698
                     /gene="Tpm4"
                     /gene_synonym="2610528G24Rik; Tpm4.2; Tpm4.2cy"
                     /inference="alignment:Splign:2.1.0"
     exon            699..2099
                     /gene="Tpm4"
                     /gene_synonym="2610528G24Rik; Tpm4.2; Tpm4.2cy"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2077..2082
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpm4"
                     /gene_synonym="2610528G24Rik; Tpm4.2; Tpm4.2cy"
                     /note="hexamer: AATAAA"
     polyA_site      2097
                     /gene="Tpm4"
                     /gene_synonym="2610528G24Rik; Tpm4.2; Tpm4.2cy"
                     /note="major polyA site"
ORIGIN      
        1 agctgtcgcc ggagcccagc agaacgattg agctatggcc ggcctcaact cactggaggc
       61 agtgaagcgc aagatccagg ccctgcagca gcaggcagac gacgcagagg atcgcgcgca
      121 aggcctgcag cgcgaactgg atggcgagcg cgagcggcgc gagaaagctg aaggagatgc
      181 agccgctctc aaccgccgca tccaactgct ggaggaggaa ctggaccggg ctcaggagca
      241 gctggccaca gccctgcaga atctggaaga ggcagagaag gctgctgatg agagtgagag
      301 aggcatgaag gtaatagaga accgagccat gaaagatgag gaaaagatgg agatcctgga
      361 gatgcagctc aaagaagcca agcacatcac tgacgaagcc gaccgcaagt atgaggaggt
      421 tgctcgtaag ttggtcatcc tggagggtga gctgaagaga gcagaggaga gggcggaggt
      481 atctgaacta aagtgtggtg acctggaaga agagctcaag aatgtaacta acaatctgaa
      541 atcactggag gctgcttctg aaaagtattc tgaaaaggag gataaatatg aagaagaaat
      601 caagcttctg tctgacaagc tgaaggaggc tgagacccga gctgagtttg cagaaaggac
      661 agtttctaaa ctggagaaga caatcgatga cctggaagaa aaacttgccc aggccaaaga
      721 agagaatgtg ggcttacatc agacactgga ccagacacta aacgaactta actgtatata
      781 aactaaacca gaagagtact gttttaacac caactccact ccagagagtg tgccctgtct
      841 tcctcttttg taagaagttc cgcttattac caggtttcca ccttgcagga aatgccaagc
      901 agataatgaa ttcatgacca aatattttgt gttagacaag ctttgagcac cagttaagtt
      961 taattctttc cttttccatt caaatggcac cagcttctct gattattctt attcttaaat
     1021 tgcatttatt cctaaaatag gcagggaatt tcacactgag tatagttgct taagtctgag
     1081 ggcatttggt tcttgggaat tagtcagccc cacactttga agcacaggct caagtatcta
     1141 atcttagctg gttgctgttg gggaagtttt gtgaggctag gggttgtcac aagaaaatat
     1201 gtggaaattt tccacccaga gcaaaaatta agtctcctat gggaagttat aaagcacaac
     1261 tagattagcc tgatcatact gagtctgctc cacacggccc tgtgcagaac agattgtctt
     1321 taagcattcc aagcgagaat gctggggtga tcattgttgc ccatgtgcct tctgactgag
     1381 ctttaatgct gttccatgtt acatctttcc ttcactccag aattgttctc tgaggagcaa
     1441 gcgtcttatt ccaggacttt tctgctgtgg tctaatgata tacccagcag ggaatatggc
     1501 agagtccaac ccttttcagc cagggctata cggcgaaact ctgtctcaaa taataaaaag
     1561 gcttagttgc cttcaataca ttgagcattg tactaatacc aagtgttggg caacaaaagg
     1621 tctctccagc agcaaagtag tgatagaaaa agaaattggc ttaacttcat ctccattgtt
     1681 cagggaacaa caaatggaag cttgactttg ccaagacttg aattccttcg ggaatgacac
     1741 taataaaaga ccaagacctc ctgattgaac tggatgtgga gtgctctgta ggctgcacat
     1801 tttttggctt tgtagttttc ctttctcagt taacatctca cactgaaaca ttccacattc
     1861 cccagcagtg cggggccaac tcaagtttac agtcctgact gaaagtcacc ctgcttctag
     1921 cttatctgaa ccctgcccct cactcctatt tattaagcat cacactacca gggagatgaa
     1981 ctttgagcaa actgcaattg ataaaatcta ctctgttctt tatgttcttg ctattgtatc
     2041 atacgtaata gtatcacttt ttctacattt tggtcaaata aatttttgca taaataatg
//
