LOCUS       NM_001293748            1137 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus tropomyosin 3, gamma (Tpm3), transcript variant 2,
            mRNA.
ACCESSION   NM_001293748
VERSION     NM_001293748.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Chaichim,C., Tomanic,T., Stefen,H., Paric,E., Gamaroff,L.,
            Suchowerska,A.K., Gunning,P.W., Ke,Y.D., Fath,T. and Power,J.
  TITLE     Overexpression of Tropomyosin Isoform Tpm3.1 Does Not Alter
            Synaptic Function in Hippocampal Neurons
  JOURNAL   Int J Mol Sci 22 (17), 9303 (2021)
   PUBMED   34502205
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Tomanic,T., Martin,C., Stefen,H., Paric,E., Gunning,P. and Fath,T.
  TITLE     Deletion of the Actin-Associated Tropomyosin Tpm3 Leads to Reduced
            Cell Complexity in Cultured Hippocampal Neurons-New Insights into
            the Role of the C-Terminal Region of Tpm3.1
  JOURNAL   Cells 10 (3), 715 (2021)
   PUBMED   33807093
  REMARK    GeneRIF: Deletion of the Actin-Associated Tropomyosin Tpm3 Leads to
            Reduced Cell Complexity in Cultured Hippocampal Neurons-New
            Insights into the Role of the C-Terminal Region of Tpm3.1.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1137)
  AUTHORS   Parreno,J., Amadeo,M.B., Kwon,E.H. and Fowler,V.M.
  TITLE     Tropomyosin 3.1 Association With Actin Stress Fibers is Required
            for Lens Epithelial to Mesenchymal Transition
  JOURNAL   Invest Ophthalmol Vis Sci 61 (6), 2 (2020)
   PUBMED   32492110
REFERENCE   4  (bases 1 to 1137)
  AUTHORS   Cheng,C., Nowak,R.B., Amadeo,M.B., Biswas,S.K., Lo,W.K. and
            Fowler,V.M.
  TITLE     Tropomyosin 3.5 protects the F-actin networks required for tissue
            biomechanical properties
  JOURNAL   J Cell Sci 131 (23) (2018)
   PUBMED   30333143
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1137)
  AUTHORS   Geeves,M.A., Hitchcock-DeGregori,S.E. and Gunning,P.W.
  TITLE     A systematic nomenclature for mammalian tropomyosin isoforms
  JOURNAL   J Muscle Res Cell Motil 36 (2), 147-153 (2015)
   PUBMED   25369766
  REMARK    Review article
REFERENCE   6  (bases 1 to 1137)
  AUTHORS   Ko,M.S., Threat,T.A., Wang,X., Horton,J.H., Cui,Y., Wang,X.,
            Pryor,E., Paris,J., Wells-Smith,J., Kitchen,J.R., Rowe,L.B.,
            Eppig,J., Satoh,T., Brant,L., Fujiwara,H., Yotsumoto,S. and
            Nakashima,H.
  TITLE     Genome-wide mapping of unselected transcripts from extraembryonic
            tissue of 7.5-day mouse embryos reveals enrichment in the t-complex
            and under-representation on the X chromosome
  JOURNAL   Hum Mol Genet 7 (12), 1967-1978 (1998)
   PUBMED   9811942
REFERENCE   7  (bases 1 to 1137)
  AUTHORS   Clayton,L. and Johnson,M.H.
  TITLE     Tropomyosin in preimplantation mouse development: identification,
            expression, and organization during cell division and polarization
  JOURNAL   Exp Cell Res 238 (2), 450-464 (1998)
   PUBMED   9473354
REFERENCE   8  (bases 1 to 1137)
  AUTHORS   Schevzov,G., Lloyd,C., Hailstones,D. and Gunning,P.
  TITLE     Differential regulation of tropomyosin isoform organization and
            gene expression in response to altered actin gene expression
  JOURNAL   J Cell Biol 121 (4), 811-821 (1993)
   PUBMED   8491774
REFERENCE   9  (bases 1 to 1137)
  AUTHORS   Takenaga,K., Nakamura,Y., Kageyama,H. and Sakiyama,S.
  TITLE     Nucleotide sequence of cDNA for nonmuscle tropomyosin 5 of mouse
            fibroblast
  JOURNAL   Biochim Biophys Acta 1087 (1), 101-103 (1990)
   PUBMED   2400784
REFERENCE   10 (bases 1 to 1137)
  AUTHORS   MacLeod,A.R. and Gooding,C.
  TITLE     Human hTM alpha gene: expression in muscle and nonmuscle tissue
  JOURNAL   Mol Cell Biol 8 (1), 433-440 (1988)
   PUBMED   3336363
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC163616.3.
            
            Transcript Variant: This variant (2, also known as variant Tpm3.12)
            differs in the 3' UTR and has multiple differences in the 3' coding
            region compared to variant Tpm3.13. The encoded isoform (Tpm3.12st,
            also known as isoform 2) is the same length as isoform Tpm3.13cy,
            but contains a distinct C-terminal segment. The encoded protein is
            the fast skeletal muscle isoform.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF317223.1, U04541.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849387, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-200               AC163616.3         180587-180786
            201-326             AC163616.3         181656-181781
            327-460             AC163616.3         194382-194515
            461-578             AC163616.3         195391-195508
            579-649             AC163616.3         195610-195680
            650-725             AC163616.3         196984-197059
            726-788             AC163616.3         197733-197795
            789-858             AC163616.3         197946-198015
            859-937             AC163616.3         198949-199027
            938-1137            AC163616.3         199867-200066
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 39.2 cM"
     gene            1..1137
                     /gene="Tpm3"
                     /gene_synonym="gamma-TM; hTM30nm; hTMnm; TM30nm; Tm5NM;
                     TMnm; Tpm-5; Tpm5; Trop-5"
                     /note="tropomyosin 3, gamma"
                     /db_xref="GeneID:59069"
                     /db_xref="MGI:MGI:1890149"
     exon            1..200
                     /gene="Tpm3"
                     /gene_synonym="gamma-TM; hTM30nm; hTMnm; TM30nm; Tm5NM;
                     TMnm; Tpm-5; Tpm5; Trop-5"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..941
                     /gene="Tpm3"
                     /gene_synonym="gamma-TM; hTM30nm; hTMnm; TM30nm; Tm5NM;
                     TMnm; Tpm-5; Tpm5; Trop-5"
                     /note="isoform Tpm3.12st is encoded by transcript variant
                     2; tropomyosin alpha-3 chain; tropomyosin-3;
                     gamma-tropomyosin; tropomyosin 5; skalphaTM.2"
                     /codon_start=1
                     /product="tropomyosin alpha-3 chain isoform Tpm3.12st"
                     /protein_id="NP_001280677.2"
                     /db_xref="GeneID:59069"
                     /db_xref="MGI:MGI:1890149"
                     /translation="MMEAIKKKMQMLKLDKENVLDRAEQAEAEQKQAEERSKQLEDEL
                     ATMQKKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVASLNRRIQLVEEELDR
                     AQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEAD
                     RKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQK
                     EDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEDELYAQKLKYKAISDELD
                     HALNDMTSI"
     exon            201..326
                     /gene="Tpm3"
                     /gene_synonym="gamma-TM; hTM30nm; hTMnm; TM30nm; Tm5NM;
                     TMnm; Tpm-5; Tpm5; Trop-5"
                     /inference="alignment:Splign:2.1.0"
     exon            327..460
                     /gene="Tpm3"
                     /gene_synonym="gamma-TM; hTM30nm; hTMnm; TM30nm; Tm5NM;
                     TMnm; Tpm-5; Tpm5; Trop-5"
                     /inference="alignment:Splign:2.1.0"
     exon            461..578
                     /gene="Tpm3"
                     /gene_synonym="gamma-TM; hTM30nm; hTMnm; TM30nm; Tm5NM;
                     TMnm; Tpm-5; Tpm5; Trop-5"
                     /inference="alignment:Splign:2.1.0"
     exon            579..649
                     /gene="Tpm3"
                     /gene_synonym="gamma-TM; hTM30nm; hTMnm; TM30nm; Tm5NM;
                     TMnm; Tpm-5; Tpm5; Trop-5"
                     /inference="alignment:Splign:2.1.0"
     exon            650..725
                     /gene="Tpm3"
                     /gene_synonym="gamma-TM; hTM30nm; hTMnm; TM30nm; Tm5NM;
                     TMnm; Tpm-5; Tpm5; Trop-5"
                     /inference="alignment:Splign:2.1.0"
     exon            726..788
                     /gene="Tpm3"
                     /gene_synonym="gamma-TM; hTM30nm; hTMnm; TM30nm; Tm5NM;
                     TMnm; Tpm-5; Tpm5; Trop-5"
                     /inference="alignment:Splign:2.1.0"
     exon            789..858
                     /gene="Tpm3"
                     /gene_synonym="gamma-TM; hTM30nm; hTMnm; TM30nm; Tm5NM;
                     TMnm; Tpm-5; Tpm5; Trop-5"
                     /inference="alignment:Splign:2.1.0"
     exon            859..937
                     /gene="Tpm3"
                     /gene_synonym="gamma-TM; hTM30nm; hTMnm; TM30nm; Tm5NM;
                     TMnm; Tpm-5; Tpm5; Trop-5"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1137
                     /gene="Tpm3"
                     /gene_synonym="gamma-TM; hTM30nm; hTMnm; TM30nm; Tm5NM;
                     TMnm; Tpm-5; Tpm5; Trop-5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1115..1120
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpm3"
                     /gene_synonym="gamma-TM; hTM30nm; hTMnm; TM30nm; Tm5NM;
                     TMnm; Tpm-5; Tpm5; Trop-5"
                     /note="hexamer: ATTAAA"
     polyA_site      1137
                     /gene="Tpm3"
                     /gene_synonym="gamma-TM; hTM30nm; hTMnm; TM30nm; Tm5NM;
                     TMnm; Tpm-5; Tpm5; Trop-5"
                     /note="major polyA site"
ORIGIN      
        1 gacccgggtg gcagcccttc ctctctggcc ccagcctcca gttctccagt gttcacaggt
       61 gagcccacca acggtcacag ctcatgatgg aggccatcaa gaaaaagatg cagatgctga
      121 agttagacaa agagaatgtt ctggaccgag ctgagcaagc tgaagctgag cagaaacagg
      181 cagaagaaag aagcaagcag ctagaggatg aactagcaac catgcagaag aagctgaaag
      241 ggacagagga tgagctggac aagtattcgg aagctttaaa ggatgctcag gagaagctgg
      301 agctagcaga gaagaaggca gccgacgctg aagctgaggt ggcctccttg aaccgcagga
      361 tccagctggt tgaagaggag ctggaccgtg cgcaggagcg ccttgccact gctttgcaga
      421 agctggagga agcagagaag gctgctgatg agagtgagag aggtatgaag gtgattgaaa
      481 atcgggctct aaaagatgaa gaaaagatgg aactccagga aatccagcta aaggaagcaa
      541 agcacattgc agaagaggcc gataggaagt atgaagaggt ggctcgtaag ttggtgatta
      601 ttgaaggaga cttggaacgc acggaggaac gtgctgagct ggcagagtct aagtgttctg
      661 agctggagga ggagctgaag aatgtcacca acaacctcaa gtctcttgag gctcaggcgg
      721 agaagtactc tcaaaaagaa gacaagtatg aagaagaaat aaagattctt actgataaac
      781 tcaaggaggc agagacccgt gctgagtttg ctgaaagatc ggtagccaag ctggagaaga
      841 ccattgatga cctggaagac gagctctatg cccagaaact gaagtacaag gccattagcg
      901 acgagctgga ccacgccctc aatgacatga cctctatata aactgtcacc gtttctgccc
      961 tgttctggat ctgccccctt tccttgggga acccaacacc ccactcgctc tggattccat
     1021 ttgggtcagc ctggctggtt cctacagcct tagggctgga gggccttggg ggggggcaca
     1081 caaagcaatt tacatatttg ctttccaacc ccatattaaa atgttaagct gccagaa
//
