LOCUS       NM_013861               2678 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Mus musculus thiamine pyrophosphokinase (Tpk1), transcript variant
            1, mRNA.
ACCESSION   NM_013861
VERSION     NM_013861.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Sahu,U., Villa,E., Reczek,C.R., Zhao,Z., O'Hara,B.P., Torno,M.D.,
            Mishra,R., Shannon,W.D., Asara,J.M., Gao,P., Shilatifard,A.,
            Chandel,N.S. and Ben-Sahra,I.
  TITLE     Pyrimidines maintain mitochondrial pyruvate oxidation to support de
            novo lipogenesis
  JOURNAL   Science 383 (6690), 1484-1492 (2024)
   PUBMED   38547260
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2678)
  AUTHORS   Liu,J.Y., Timm,D.E. and Hurley,T.D.
  TITLE     Pyrithiamine as a substrate for thiamine pyrophosphokinase
  JOURNAL   J Biol Chem 281 (10), 6601-6607 (2006)
   PUBMED   16365036
  REMARK    GeneRIF: Three-dimensional structure of thiamine pyrophosphokinase
            crystallized from an equilibrium substrate/product mixture shows
            clear electron density for pyrithiamine pyrophosphate bound in the
            enzyme active site.
REFERENCE   4  (bases 1 to 2678)
  AUTHORS   Timm,D.E., Liu,J., Baker,L.J. and Harris,R.A.
  TITLE     Crystal structure of thiamin pyrophosphokinase
  JOURNAL   J Mol Biol 310 (1), 195-204 (2001)
   PUBMED   11419946
REFERENCE   5  (bases 1 to 2678)
  AUTHORS   Zhao,R., Gao,F. and Goldman,I.D.
  TITLE     Molecular cloning of human thiamin pyrophosphokinase
  JOURNAL   Biochim Biophys Acta 1517 (2), 320-322 (2001)
   PUBMED   11342117
REFERENCE   6  (bases 1 to 2678)
  AUTHORS   Nosaka,K., Onozuka,M., Kakazu,N., Hibi,S., Nishimura,H., Nishino,H.
            and Abe,T.
  TITLE     Isolation and characterization of a human thiamine
            pyrophosphokinase cDNA
  JOURNAL   Biochim Biophys Acta 1517 (2), 293-297 (2001)
   PUBMED   11342111
REFERENCE   7  (bases 1 to 2678)
  AUTHORS   Nosaka,K., Onozuka,M., Nishino,H., Nishimura,H., Kawasaki,Y. and
            Ueyama,H.
  TITLE     Molecular cloning and expression of a mouse thiamin
            pyrophosphokinase cDNA
  JOURNAL   J Biol Chem 274 (48), 34129-34133 (1999)
   PUBMED   10567383
REFERENCE   8  (bases 1 to 2678)
  AUTHORS   Bettendorff,L. and Wins,P.
  TITLE     Mechanism of thiamine transport in neuroblastoma cells. Inhibition
            of a high affinity carrier by sodium channel activators and
            dependence of thiamine uptake on membrane potential and
            intracellular ATP
  JOURNAL   J Biol Chem 269 (20), 14379-14385 (1994)
   PUBMED   8182042
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK087844.1, AK052225.1, AK035044.1 and BE691023.1.
            
            On Jul 24, 2015 this sequence version replaced NM_013861.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK087844.1, BC015246.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-114               AK087844.1         2-115
            115-309             AK052225.1         3-197
            310-2532            AK052225.1         199-2421
            2533-2663           AK035044.1         2431-2561
            2664-2678           BE691023.1         1-15                c
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 21.19 cM"
     gene            1..2678
                     /gene="Tpk1"
                     /note="thiamine pyrophosphokinase"
                     /db_xref="GeneID:29807"
                     /db_xref="MGI:MGI:1352500"
     exon            1..143
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            144..201
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            202..273
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            274..343
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            344..416
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            417..512
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            513..659
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            660..771
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     exon            772..2671
                     /gene="Tpk1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2512..2517
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpk1"
                     /note="hexamer: AATAAA"
     polyA_site      2533
                     /gene="Tpk1"
                     /note="major polyA site"
ORIGIN      
        1 agaggcgggg ctctgtgctc gcgcacgaag ccctctcccg cgaacgcgcg gcgcgcccca
       61 ggctccgcag ccgagcgccg agctttcgat ccgtcactcc cgcagccctc gctcgcgtcg
      121 ctcgtggctc cacccgccat tgtaatccat tccccgctat ggagcatgcc tttaccccgt
      181 tggaacccct gctacctacg gggaacttga aatactgcct tgtggttctt aatcagcctt
      241 tggatgcacg atttcgccat ctttggaaaa aagctctctt aagagcctgt gctgatgggg
      301 gtgccaacca cttatatgat ctcactgaag gagagagaga aagcttcttg cctgaattcg
      361 tcagtgggga ctttgattct attaggcctg aagtcaaaga gtactacacc aagaagggct
      421 gtgatcttat ttcaactcct gaccaagacc acactgactt taccaagtgt cttcaagtgc
      481 tccaaaggaa gatagaggaa aaggaactgc aggttgacgt gattgtgaca ctgggaggtc
      541 tcggtgggcg ttttgaccaa atcatggcct ctgtgaatac ccttttccaa gccactcaca
      601 tcactcctgt gccgattata ataatccaaa aggactctct catctacctc ctccaacccg
      661 ggaagcacag gctccatgta gacactggaa tggaagggag ctggtgtggc ctgattcctg
      721 ttggacagcc ttgcaaccag gtgacgacaa caggcctgaa atggaacctc acaaatgatg
      781 ttcttggctt tggaacactg gtcagtactt ctaacaccta cgatgggtcc ggccttgtca
      841 ctgtggaaac tgaccaccca ctcctctgga ccatggccat caagagctaa cctacagtgc
      901 ggcatccatc ttgtgcctgt gccttccctt acctgcccac tggttcgttg ctcagcatga
      961 acagccacat aggtgtgcag aaatgtaaac ttctccatgg ggaaccatga gctttcaagg
     1021 tgttggggtt aaggtatagc tgagtggcag agcacttgtt gcgcatgtac aaggccttgg
     1081 gttcaaccct ccacaccaca aaataaagat ggtggtgttt aaagattcca ggtaatgcta
     1141 taaatgatat ctcaatgtta tattgaggga aaaattatgg tctatcaaag caaataagct
     1201 gtattgtatg gctaatatag ctaatgagga tcattacatt atgtaattca gttttgatca
     1261 ttttaattta gttaatcagt ccatttacct taaaattctt tttttaaatt ttgaagcacc
     1321 agaagctttt ttattgtgtt gccttggaat ctggcacttc cttaagtgcc tgacaacaag
     1381 ggctgcctcc tctgagtagc tcctaacatc agactccggt ctgtaatgta caatagctcc
     1441 tcgagtgctg tgatagaatg gcaccagttc actttgacat catcttttca aagataagaa
     1501 tcattgactt aattgttagg tttaaaagca agcagctaat gggcttcggg taaatgagaa
     1561 atggcaattc aggattcatt caaacattaa cccctgaaaa cgtgaaagca cgctctctga
     1621 gctctaggaa tgtgggtgcc cttatgaaaa ttgtgacaca ctcaaaggct ttggcctgag
     1681 gaatcgatca tgagcctgga aattcagtca cttttaacct atttcccact aggagcagga
     1741 aagatggctc agcggttagg gacactagat actcttccag aggtcttgaa ttcagttccc
     1801 accaactacg tggtagcttg catccatcta taatggaatc cgataccctc ttatggaatg
     1861 caggtgtaca tgcagagaga atactcataa acataaaaat aaataaattt ggagggaaaa
     1921 cacacttcac gctaaaaagc aaaatattac tccatggacc atatctactg acattaccag
     1981 aaaattagtg tgactatgga gataaatgaa taccacttgc tgaggagtca acaactgaag
     2041 cctgtttctg cattccagtc caccatgact cgctctctct gctgtatggt gggaggaagt
     2101 actcactgag tgtgtgaaag aaccacaggt ataaactcta aatgctaacg tgttgcaggt
     2161 gtagagtaaa taacacagtc cctgacttag cctaagcttg ctggtgcatt aaaacttatt
     2221 catgtttctt tcatgagctc ccccctcatc ttcctaccca tttcccctca tcttcttgtc
     2281 ccatttctta aaccgtatct tgccaaacaa accatgattt aaggcaacct gcctaccttg
     2341 ttgcatttat ccaatgacta tattactgtc ttcttggtgc tacaacagaa tgaagcttaa
     2401 aacaaataga ggaagaaaag ggatcagttg aatcaatacc aagttcttaa cagaaatagt
     2461 tagagtcatt attattttat gcaaatatat gattctgaaa tctgaatgtc aaataaaaag
     2521 ttatcaatgt gcatgtctgt agttggtttg tcagaaatgt ttaatgttat cgcttgtaat
     2581 tcaacatgac taatctttca taatcattgt ttctgttcaa aaggtgaatt actatgtgta
     2641 ttttataatt aaaatctcag atctgacttc aaaaaaaa
//
