LOCUS       NM_001142698            3607 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus tubulin polyglutamylase complex subunit 2 (Tpgs2),
            transcript variant 3, mRNA.
ACCESSION   NM_001142698
VERSION     NM_001142698.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Sundberg,J.P., Dadras,S.S., Silva,K.A., Kennedy,V.E., Garland,G.,
            Murray,S.A., Sundberg,B.A., Schofield,P.N. and Pratt,C.H.
  TITLE     Systematic screening for skin, hair, and nail abnormalities in a
            large-scale knockout mouse program
  JOURNAL   PLoS One 12 (7), e0180682 (2017)
   PUBMED   28700664
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3607)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 3607)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 3607)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   6  (bases 1 to 3607)
  AUTHORS   Janke,C., Rogowski,K., Wloga,D., Regnard,C., Kajava,A.V.,
            Strub,J.M., Temurak,N., van Dijk,J., Boucher,D., van Dorsselaer,A.,
            Suryavanshi,S., Gaertig,J. and Edde,B.
  TITLE     Tubulin polyglutamylase enzymes are members of the TTL domain
            protein family
  JOURNAL   Science 308 (5729), 1758-1762 (2005)
   PUBMED   15890843
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BQ770487.1, BC060253.1, AK141632.1 and AC124441.5.
            
            Transcript Variant: This variant (3) differs in the 3' UTR and 3'
            coding region, compared to variant 1. The resulting isoform (c) is
            shorter and has a distinct C-terminus, compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784647.1308267.1,
                                           SRR17784643.728914.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-563               BQ770487.1         1-563
            564-666             BC060253.1         116-218
            667-3085            AK141632.1         484-2902
            3086-3607           AC124441.5         35073-35594
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 13.69 cM"
     gene            1..3607
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="tubulin polyglutamylase complex subunit 2"
                     /db_xref="GeneID:66648"
                     /db_xref="MGI:MGI:1913898"
     exon            1..351
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     CDS             270..995
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="isoform c is encoded by transcript variant 3"
                     /codon_start=1
                     /product="tubulin polyglutamylase complex subunit 2
                     isoform c"
                     /protein_id="NP_001136170.1"
                     /db_xref="CCDS:CCDS50240.1"
                     /db_xref="GeneID:66648"
                     /db_xref="MGI:MGI:1913898"
                     /translation="MEETTPPLQAGSKPHLEKLTLGVTRILESSPGVTEVSIIEKLPA
                     ERHMISSWEQKNNCVMPEDVRNFYLMTNGFHMTWSVKLDEHIIPLGSMVINGISKLTQ
                     LIQSSVYSLPNAPTLADLEDDTQEGNEDHQLEKPHFDCRSAIFELDSCNGNGKVCLVY
                     KNGKPGLAHDTEIWFLDRALYWHFLTDTFTAYYRLLITHLGLPQWQYAFTSYGISPQA
                     KVASIYSQEESMNFQATSVRNQW"
     exon            352..431
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            432..519
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            520..648
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            649..765
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            766..926
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            927..3607
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1299..1304
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="hexamer: AATAAA"
     polyA_site      1321
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="major polyA site"
ORIGIN      
        1 cgcggagatg tgaagcgaag cccgggccag ggtcgcggga acgagctccc ggcggaagtg
       61 ccgctgccaa agccacccgg gccgcccatc ctctcctggc ggagccctca ggcgctgaaa
      121 actacaactc ccggcatgcc ctgagcccga cggccgcaga cctgaggcgg ggtcgggcgc
      181 gtcagccggc aggccggctg tccacggcgg cgccggggac tggggccgcc gccgccgccg
      241 ccgctgcgcg cggtgtgagg acgcgtgcca tggaggagac aacgccccca ctgcaagccg
      301 gcagcaagcc gcaccttgag aagttgaccc taggcgtcac tcgcatcctg gaatcttccc
      361 caggtgtgac cgaggtgagc ataatagaaa agctgcctgc tgagcgtcat atgatttctt
      421 catgggaaca gaaaaacaat tgtgtgatgc ctgaggatgt gagaaacttc tacttgatga
      481 ccaatggttt tcacatgacc tggagtgtga agctagatga gcacattatt ccactgggaa
      541 gcatggtgat taacggcatt tcaaaactga ctcagctcat ccagtcttct gtgtactcac
      601 ttcctaatgc accaactctg gcagacctag aggacgatac tcaagaagga aatgaagacc
      661 accagctaga gaagcctcac tttgactgtc gcagtgcaat atttgagctg gattcatgca
      721 atgggaatgg gaaagtttgc ctcgtctaca aaaatgggaa acctgggtta gctcatgaca
      781 ctgagatatg gttcctggac agagcattat actggcactt tctcacggat acctttaccg
      841 cctattaccg cctgctcatc acccacctgg gcctgcccca gtggcagtat gcctttacca
      901 gctatggcat tagcccacag gccaaggtag cttctatcta tagtcaagaa gaaagcatga
      961 atttccaagc aacatctgtt agaaaccaat ggtgatttaa catctaggac catggccttt
     1021 gtggcagatt aacatccttg tgaaaactct tggatgatga agtttcttca gaaggaaaga
     1081 tactgggggt atcccaaccc attcatctct ataccttaaa cttcattttc taatagatgt
     1141 acagttctac aagcctacag gttatagttc tctaattcat agccactgat attgtgcctg
     1201 agtaagggct agaggaagcc aaagaaatgc caccctgctc aatctgaggg aggtaaatga
     1261 tttttaaatg ttttttttgt taaatgaagg ttactgttaa taaacattat gttaatatgc
     1321 atatatctta tgtctccttt cttactgtta tttttaaggt tctgaggctt gtaagtacct
     1381 agctgttaat gctctagaac ttgattgaac tagctgagaa caatgaatca ttgtttagta
     1441 aactatgatt tttatgggca tgtgtgcaca agcatacatg tgtatgtgtg agtgtgttta
     1501 tgcatgtgtg tatgtgtgtg tgcataagca tatatgtatt tgtatgtatg tgtccataca
     1561 tgcctgtatg tccatgtgtg tatatgtgag cttgaactct tactataaat atgaaagagg
     1621 ataaggacag tatcgtcaag tggcaatggc tgttataatt actgacacgg tttttaaatt
     1681 attctaatta tttatattca aaatgttgca cctccttcca gtctgctatc ccagagtcct
     1741 ttgccccacc ctccctccct ttgcctctaa gagggtgctc cctcccgcca agcatctccc
     1801 tttcctgggg catcaagtct ctacaggatt aggtacatcc tctccccctg aagccagaca
     1861 aggcagtcct ctgatacata tgttccaggg gcctcagctc agctcaagta tactctttgg
     1921 ttggtggctt agtctctggc agctcccagg gatctaggtt agttgacatt gttggtcttc
     1981 ctatggggtt gccatcccct tcagctccgt caatccttct cctaactctt ccataggtgt
     2041 cctgacctca gtccaatggt aggctgtgag tatctgcatc tgtctcagtc agctgctggt
     2101 aaagtctctc agaggacagc catgctaggc tcctgtctgc aagcacaaca tagcatcagt
     2161 aatagtgtca agatttggtg ccctcccatg ggatagatcc caagttgggc cagtcactgg
     2221 atggcctttc tttcagtctc tgctccattt ttgtccctac atttctttta gacaagaaca
     2281 attccagatc aaaatttttg gaggtgggtt ggtaacccca tccctctact ggggaacctg
     2341 tctatctact ggaggtggtc tcttcaggtt ccatctccct actgctgggc atttcagcta
     2401 aggtcatccc caattaggct gacacagttt ccaaagtcta cttttgctta gatttcttaa
     2461 tggctgtcct cagagtagag ccaccaaaca gcaaaactgt atcatgcagc agttgtctga
     2521 ttcctttcca ccagctgatt taaaattctg tatgaaatct aaactatcct taccaaccca
     2581 cacacaactc catcaattcc atttgctcct gatatacttt gagaacactg gacagaaatg
     2641 caggtatgaa gtgaccctcc cctctgagcc ttaccagcat ttctcagtga ctcggttttg
     2701 atgtagcagt taatgagaaa cctaaagcat taccaggctg ttctgtgaca actttatctc
     2761 cttgtgacct cattgtggaa ttgatttctt gtttgcatct tctctttcct tggtcacttc
     2821 tccttgctac ctggttacca cttataatca ttctttctgc ttatatacat gctcttagaa
     2881 cctctgcacc tccatgcaga cattggagaa agactgccat ggcctctcat gcatccaatg
     2941 gcctagaaga ctcactgggc tattccaagg cattagacat agtggaggga tgcagcattc
     3001 tagaccataa cagcacgata tcagtttacc ttcacttcat ttctcacgct gtgtaattgt
     3061 ttgaaagtaa acagacattt atgtttccct tttagaaatc taaccttaaa cagcctacaa
     3121 agggcaagtc atgtcaggag aggtgttgat ttaatacaga gaaatgtttg ttttcactct
     3181 gaaggataat ccccaaaggg attatctagc tagaggagaa ctttctctgg cattgaaaga
     3241 tgtcaatctg cctttttgct ttgttttgtt ttgcttaaat cttctatagt ttctgttgaa
     3301 aaaatgaacc atcatttcta cacccaggct ataggtaaaa agtgtctttt ctctgttatg
     3361 aaaatattat ctttatgtgt ggttttcagc agtatgccta tgaaattttt ttattatgta
     3421 cttagtctgc ttatttcttt tatgtgtggg ggtgttttat atgtatgtat gtgtatgcat
     3481 cgtgtatatt cctggtatct gaagccaaaa gtggtcatgg atcccctgag aatggagttt
     3541 gagatatctg tgagctgcca tgtgtgtgct gcgaaaggct aaacagtgat ctaacagctg
     3601 aaccatc
//
