LOCUS       NM_001142697            3786 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus tubulin polyglutamylase complex subunit 2 (Tpgs2),
            transcript variant 2, mRNA.
ACCESSION   NM_001142697
VERSION     NM_001142697.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Sundberg,J.P., Dadras,S.S., Silva,K.A., Kennedy,V.E., Garland,G.,
            Murray,S.A., Sundberg,B.A., Schofield,P.N. and Pratt,C.H.
  TITLE     Systematic screening for skin, hair, and nail abnormalities in a
            large-scale knockout mouse program
  JOURNAL   PLoS One 12 (7), e0180682 (2017)
   PUBMED   28700664
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3786)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 3786)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 3786)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   6  (bases 1 to 3786)
  AUTHORS   Janke,C., Rogowski,K., Wloga,D., Regnard,C., Kajava,A.V.,
            Strub,J.M., Temurak,N., van Dijk,J., Boucher,D., van Dorsselaer,A.,
            Suryavanshi,S., Gaertig,J. and Edde,B.
  TITLE     Tubulin polyglutamylase enzymes are members of the TTL domain
            protein family
  JOURNAL   Science 308 (5729), 1758-1762 (2005)
   PUBMED   15890843
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DV655125.1, AC124441.5 and AI666318.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation from a
            downstream start codon, compared to variant 1. The resulting
            isoform (b) is shorter at the N-terminus, compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DV655125.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164141 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-678               DV655125.1         1-678
            679-3655            AC124441.5         23684-26660
            3656-3786           AI666318.1         1-131               c
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 13.69 cM"
     gene            1..3786
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="tubulin polyglutamylase complex subunit 2"
                     /db_xref="GeneID:66648"
                     /db_xref="MGI:MGI:1913898"
     exon            1..97
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            98..177
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..906
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="isoform b is encoded by transcript variant 2"
                     /codon_start=1
                     /product="tubulin polyglutamylase complex subunit 2
                     isoform b"
                     /protein_id="NP_001136169.1"
                     /db_xref="GeneID:66648"
                     /db_xref="MGI:MGI:1913898"
                     /translation="MISSWEQKNNCVMPEDVRNFYLMTNGFHMTWSVKLDEHIIPLGS
                     MVINGISKLTQLIQSSVYSLPNAPTLADLEDDTQEGNEDHQLEKPHFDCRSAIFELDS
                     CNGNGKVCLVYKNGKPGLAHDTEIWFLDRALYWHFLTDTFTAYYRLLITHLGLPQWQY
                     AFTSYGISPQAKQWFSMYKPITYNTSLLTEESDNFVNKLDPSKVFKSKNKILIPKKKG
                     PVPPASGQKGPGPLPPPTSKPTTGSGNPVRK"
     exon            178..265
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            266..394
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            395..511
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            512..672
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            673..3775
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1176..1181
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="hexamer: AATAAA"
     polyA_site      1201
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="major polyA site"
     regulatory      1580..1585
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="hexamer: AATAAG"
     polyA_site      1600
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
     regulatory      3661..3666
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="hexamer: AATAAA"
     polyA_site      3689
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
     regulatory      3749..3754
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="hexamer: AATAAA"
     polyA_site      3775
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
ORIGIN      
        1 aagaacatct ctccatggct tctgcatcag ctcctgtttc ctgacctgct tgagttccag
       61 tcctgacttc cttggtgatg agcagcagta tggaagtaat cttccccagg tgtgaccgag
      121 gtgagcataa tagaaaagct gcctgctgag cgtcatatga tttcttcatg ggaacagaaa
      181 aacaattgtg tgatgcctga ggatgtgaga aacttctact tgatgaccaa tggttttcac
      241 atgacctgga gtgtgaagct agatgagcac attattccac tgggaagcat ggtgattaac
      301 ggcatttcaa aactgactca gctcatccag tcttctgtgt actcacttcc taatgcacca
      361 actctggcag acctagagga cgatactcaa gaaggaaatg aagaccacca gctagagaag
      421 cctcactttg actgtcgcag tgcaatattt gagctggatt catgcaatgg gaatgggaaa
      481 gtttgcctcg tctacaaaaa tgggaaacct gggttagctc atgacactga gatatggttc
      541 ctggacagag cattatactg gcactttctc acggatacct ttaccgccta ttaccgcctg
      601 ctcatcaccc acctgggcct gccccagtgg cagtatgcct ttaccagcta tggcattagc
      661 ccacaggcca agcaatggtt cagtatgtac aaacccatca cctacaacac aagcctgctc
      721 acagaagagt ccgacaactt tgtgaacaag ctagacccca gcaaagtgtt caagagcaag
      781 aacaagattt taatcccaaa aaagaaaggg cctgtaccac ctgccagtgg tcagaaaggg
      841 cctgggccct tacctcctcc tacctctaag cctaccacag gctctggaaa tcctgtccga
      901 aaataagcat tctatcacct cagtaatatc cagacacaga tggccctgtg gcagcggcat
      961 tctcctgaga gaccaaagcc ttaggccttt gtttccactg tcattagcta ggggctggga
     1021 cgatcacact gaattctcct caggagttcc ttcctctgta ggttgtgaca acctcatctc
     1081 caaagccaac gaagactttc agatgtacct gagctttctt atgtctgtac ccaatcctta
     1141 acttcttagc agtaattaga gaggtcaccc tcccaaataa acccatggtt tgactcatga
     1201 agcatcagta tcgactagtt atgtgaccat ctcagttaat gaaaattgca caccttcagt
     1261 tgcattcacc acacttcagc tatgcacctg atggtagagt ccatattgga cagcacagaa
     1321 aggggaaggt ttttgtcaca gaagtgtttt ggacaacatt gcatgtgaac acgttcatac
     1381 tgtccttcag atacaataac gtaatgcctt agtttcatac gctttatgtt ttgagaaaac
     1441 attttcaaag acagtcttgt gttctttaca acctagacca ggaatgctta aaggtgtgat
     1501 ccctagactt agaattgtgc accaaagtca ctggtatcat gtggggataa gatgattgga
     1561 ttccagttct gatctgcaaa ataagaattt ccaagaaaaa ttccaggtgt cagggttttc
     1621 tttttaagac aaggtctcac tatatagttt aggctgacct atagctcaca caccctatat
     1681 agcttcctga cacacacaca cacaccctgt cactaacgtt ttaattacag atgtacagta
     1741 tcatgcgtaa cattggattt ttagtcaatt taacagtact tctgagtttg aaactttcag
     1801 cttaagatct cagtggtttc aaaccacaaa gtttctttgt aattgggtaa agcacgtata
     1861 acttttttgt tttttttttt ttgttttttt ttttttgaaa cagggttttt ctgtgtagtc
     1921 ctggctgtcc tggaactcat ggagttgtgg ctgctaaatg acttagtgca tagagttagt
     1981 tatcctgtcc ttcctttgta atgtgaccct acggttgcta attatgacct tcctgctagc
     2041 agctgtgatc tcttaagtcg ttcatttttt gcttgcaaga caccagttgg gttctcactt
     2101 gtgcctttat acctgtcctt tccttgtgag gttcagctct agtctgcctc tactcaaagc
     2161 tcagtgaagc tccaactccc ctaggtgagc agtgattttt ttgtgcttct ccagaaagcc
     2221 atcctaatca attctgcatt aacccaaata aatgtgtcat attttagggc aataaattag
     2281 aatgaatgag taaagttgtg aaaatgttta actttaaaaa ttgagaagac aataattgcc
     2341 ttattttaga gtctggatat ctttcactca tcttacggtc tgtgtcacaa aaccatcaaa
     2401 atgaaatagg ttacacatga agccatgaat gccactggtc ttttctgcac ccagaaacta
     2461 tgttgtaccc agatactacc taactggata tcccaccaat aactgtcttg ataaggctct
     2521 aaaagaccaa aaagtaactc gtgagacatg gtgggatgtg aaacttctta ccctgtatct
     2581 aggaaaagtt cttcaacacc attgatgctg tttcttcaga tgtaaaatga aaatttatgg
     2641 aagggctata aaactattaa ataatgcaaa tggagtacac atggagatgc tttcagtaag
     2701 caacagcttt taaaatagca ttcagaaagt ccattttctc cccatgaatt acctaaccaa
     2761 cagaaccaat tgctttacca gatgattttt tgacaaaatt gattatttta acacctatct
     2821 ttactgtcta tttcaaagcc attagctttc ttataaaatc gcaaagacat ttcattcctg
     2881 aagcaatttg gtacttttgt ggtgtagtta cctctctgat aaaatgattt ctaatcaaaa
     2941 ctagaaatag ctaaagaatg ggggataact gaaatttaat tggatgacag cctcccacag
     3001 cttctgttag acctcctgag gagaaggatc aaggctctca ttttcccaac tgaccaccca
     3061 gccctgtcac agagctacct acaaacctgg cccttagtgc atagtttgtt ctactgtgtt
     3121 ttcaggacct gatggcttaa tacacataag tttacccgtg tgtatttctg caggccagaa
     3181 gttcaatgtc agttttatag agccaaaatc tagttgtcac tgggccagga cttgggaggc
     3241 tcctatagag tctgtccctg cttcctcctg cagattgttg tttgggttca tatcacttca
     3301 gtcctgttgc tttcacttcg tcttctcttt ctccaaatct cttctgactg tcctttataa
     3361 ggacacttat gattggattt gaggcatgtc ggacactaca ggatgatctt ctcacacctg
     3421 caaggaatcc ttacaaagga attgtacatt tcagagatta gaaccggagg ccccagagat
     3481 gttgagtcaa tgtattacag tagtgtttgc tacttcacca tttcaaggga ctgtggatat
     3541 aaatgattgt tttgaaggcc ataacggatc tataagccac tttagagctg ggaaactaag
     3601 ctgccgtttg tacccctgct catcttctct acattgtgtt caacaatgaa ccttgaagaa
     3661 aataaaaccc aggtctccag ccttccccag ccccgtgcat gcgcattctc cttcaccccc
     3721 aattcctttt gagtaagtta cttgttaaaa taaatcctta aggttcaaaa caaaaaaaaa
     3781 aaaaaa
//
