LOCUS       NM_001004361            4040 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus tubulin polyglutamylase complex subunit 2 (Tpgs2),
            transcript variant 1, mRNA.
ACCESSION   NM_001004361 XM_203706
VERSION     NM_001004361.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Sundberg,J.P., Dadras,S.S., Silva,K.A., Kennedy,V.E., Garland,G.,
            Murray,S.A., Sundberg,B.A., Schofield,P.N. and Pratt,C.H.
  TITLE     Systematic screening for skin, hair, and nail abnormalities in a
            large-scale knockout mouse program
  JOURNAL   PLoS One 12 (7), e0180682 (2017)
   PUBMED   28700664
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4040)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 4040)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 4040)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   6  (bases 1 to 4040)
  AUTHORS   Janke,C., Rogowski,K., Wloga,D., Regnard,C., Kajava,A.V.,
            Strub,J.M., Temurak,N., van Dijk,J., Boucher,D., van Dorsselaer,A.,
            Suryavanshi,S., Gaertig,J. and Edde,B.
  TITLE     Tubulin polyglutamylase enzymes are members of the TTL domain
            protein family
  JOURNAL   Science 308 (5729), 1758-1762 (2005)
   PUBMED   15890843
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BQ770487.1, BC060253.1, AK017728.1, AC124441.5 and AI666318.1.
            
            On Dec 17, 2008 this sequence version replaced NM_001004361.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660817.200620.1,
                                           SRR1660821.181057.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164141
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-563               BQ770487.1         1-563
            564-1455            BC060253.1         116-1007
            1456-1847           AK017728.1         1208-1599
            1848-3909           AC124441.5         24599-26660
            3910-4040           AI666318.1         1-131               c
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 13.69 cM"
     gene            1..4040
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="tubulin polyglutamylase complex subunit 2"
                     /db_xref="GeneID:66648"
                     /db_xref="MGI:MGI:1913898"
     exon            1..351
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     CDS             270..1160
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="isoform a is encoded by transcript variant 1"
                     /codon_start=1
                     /product="tubulin polyglutamylase complex subunit 2
                     isoform a"
                     /protein_id="NP_001004361.2"
                     /db_xref="CCDS:CCDS29105.2"
                     /db_xref="GeneID:66648"
                     /db_xref="MGI:MGI:1913898"
                     /translation="MEETTPPLQAGSKPHLEKLTLGVTRILESSPGVTEVSIIEKLPA
                     ERHMISSWEQKNNCVMPEDVRNFYLMTNGFHMTWSVKLDEHIIPLGSMVINGISKLTQ
                     LIQSSVYSLPNAPTLADLEDDTQEGNEDHQLEKPHFDCRSAIFELDSCNGNGKVCLVY
                     KNGKPGLAHDTEIWFLDRALYWHFLTDTFTAYYRLLITHLGLPQWQYAFTSYGISPQA
                     KQWFSMYKPITYNTSLLTEESDNFVNKLDPSKVFKSKNKILIPKKKGPVPPASGQKGP
                     GPLPPPTSKPTTGSGNPVRK"
     exon            352..431
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            432..519
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            520..648
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            649..765
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            766..926
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     exon            927..4029
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1430..1435
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="hexamer: AATAAA"
     polyA_site      1455
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="major polyA site"
     regulatory      1834..1839
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="hexamer: AATAAG"
     polyA_site      1854
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
     regulatory      3915..3920
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="hexamer: AATAAA"
     polyA_site      3943
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
     regulatory      4003..4008
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
                     /note="hexamer: AATAAA"
     polyA_site      4029
                     /gene="Tpgs2"
                     /gene_synonym="5730437P09Rik; 5730494M16Rik; Pgs2"
ORIGIN      
        1 cgcggagatg tgaagcgaag cccgggccag ggtcgcggga acgagctccc ggcggaagtg
       61 ccgctgccaa agccacccgg gccgcccatc ctctcctggc ggagccctca ggcgctgaaa
      121 actacaactc ccggcatgcc ctgagcccga cggccgcaga cctgaggcgg ggtcgggcgc
      181 gtcagccggc aggccggctg tccacggcgg cgccggggac tggggccgcc gccgccgccg
      241 ccgctgcgcg cggtgtgagg acgcgtgcca tggaggagac aacgccccca ctgcaagccg
      301 gcagcaagcc gcaccttgag aagttgaccc taggcgtcac tcgcatcctg gaatcttccc
      361 caggtgtgac cgaggtgagc ataatagaaa agctgcctgc tgagcgtcat atgatttctt
      421 catgggaaca gaaaaacaat tgtgtgatgc ctgaggatgt gagaaacttc tacttgatga
      481 ccaatggttt tcacatgacc tggagtgtga agctagatga gcacattatt ccactgggaa
      541 gcatggtgat taacggcatt tcaaaactga ctcagctcat ccagtcttct gtgtactcac
      601 ttcctaatgc accaactctg gcagacctag aggacgatac tcaagaagga aatgaagacc
      661 accagctaga gaagcctcac tttgactgtc gcagtgcaat atttgagctg gattcatgca
      721 atgggaatgg gaaagtttgc ctcgtctaca aaaatgggaa acctgggtta gctcatgaca
      781 ctgagatatg gttcctggac agagcattat actggcactt tctcacggat acctttaccg
      841 cctattaccg cctgctcatc acccacctgg gcctgcccca gtggcagtat gcctttacca
      901 gctatggcat tagcccacag gccaagcaat ggttcagtat gtacaaaccc atcacctaca
      961 acacaagcct gctcacagaa gagtccgaca actttgtgaa caagctagac cccagcaaag
     1021 tgttcaagag caagaacaag attttaatcc caaaaaagaa agggcctgta ccacctgcca
     1081 gtggtcagaa agggcctggg cccttacctc ctcctacctc taagcctacc acaggctctg
     1141 gaaatcctgt ccgaaaataa gcattctatc acctcagtaa tatccagaca cagatggccc
     1201 tgtggcagcg gcattctcct gagagaccaa agccttaggc ctttgtttcc actgtcatta
     1261 gctaggggct gggacgatca cactgaattc tcctcaggag ttccttcctc tgtaggttgt
     1321 gacaacctca tctccaaagc caacgaagac tttcagatgt acctgagctt tcttatgtct
     1381 gtacccaatc cttaacttct tagcagtaat tagagaggtc accctcccaa ataaacccat
     1441 ggtttgactc atgaagcatc agtatcgact agttatgtga ccatctcagt taatgaaaat
     1501 tgcacacctt cagttgcatt caccacactt cagctatgca cctgatggta gagtccatat
     1561 tggacagcac agaaagggga aggtttttgt cacagaagtg ttttggacaa cattgcatgt
     1621 gaacacgttc atactgtcct tcagatacaa taacgtaatg ccttagtttc atacgcttta
     1681 tgttttgaga aaacattttc aaagacagtc ttgtgttctt tacaacctag accaggaatg
     1741 cttaaaggtg tgatccctag acttagaatt gtgcaccaaa gtcactggta tcatgtgggg
     1801 ataagatgat tggattccag ttctgatctg caaaataaga atttccaaga aaaattccag
     1861 gtgtcagggt tttcttttta agacaaggtc tcactatata gtttaggctg acctatagct
     1921 cacacaccct atatagcttc ctgacacaca cacacacacc ctgtcactaa cgttttaatt
     1981 acagatgtac agtatcatgc gtaacattgg atttttagtc aatttaacag tacttctgag
     2041 tttgaaactt tcagcttaag atctcagtgg tttcaaacca caaagtttct ttgtaattgg
     2101 gtaaagcacg tataactttt ttgttttttt ttttttgttt tttttttttt gaaacagggt
     2161 ttttctgtgt agtcctggct gtcctggaac tcatggagtt gtggctgcta aatgacttag
     2221 tgcatagagt tagttatcct gtccttcctt tgtaatgtga ccctacggtt gctaattatg
     2281 accttcctgc tagcagctgt gatctcttaa gtcgttcatt ttttgcttgc aagacaccag
     2341 ttgggttctc acttgtgcct ttatacctgt cctttccttg tgaggttcag ctctagtctg
     2401 cctctactca aagctcagtg aagctccaac tcccctaggt gagcagtgat ttttttgtgc
     2461 ttctccagaa agccatccta atcaattctg cattaaccca aataaatgtg tcatatttta
     2521 gggcaataaa ttagaatgaa tgagtaaagt tgtgaaaatg tttaacttta aaaattgaga
     2581 agacaataat tgccttattt tagagtctgg atatctttca ctcatcttac ggtctgtgtc
     2641 acaaaaccat caaaatgaaa taggttacac atgaagccat gaatgccact ggtcttttct
     2701 gcacccagaa actatgttgt acccagatac tacctaactg gatatcccac caataactgt
     2761 cttgataagg ctctaaaaga ccaaaaagta actcgtgaga catggtggga tgtgaaactt
     2821 cttaccctgt atctaggaaa agttcttcaa caccattgat gctgtttctt cagatgtaaa
     2881 atgaaaattt atggaagggc tataaaacta ttaaataatg caaatggagt acacatggag
     2941 atgctttcag taagcaacag cttttaaaat agcattcaga aagtccattt tctccccatg
     3001 aattacctaa ccaacagaac caattgcttt accagatgat tttttgacaa aattgattat
     3061 tttaacacct atctttactg tctatttcaa agccattagc tttcttataa aatcgcaaag
     3121 acatttcatt cctgaagcaa tttggtactt ttgtggtgta gttacctctc tgataaaatg
     3181 atttctaatc aaaactagaa atagctaaag aatgggggat aactgaaatt taattggatg
     3241 acagcctccc acagcttctg ttagacctcc tgaggagaag gatcaaggct ctcattttcc
     3301 caactgacca cccagccctg tcacagagct acctacaaac ctggccctta gtgcatagtt
     3361 tgttctactg tgttttcagg acctgatggc ttaatacaca taagtttacc cgtgtgtatt
     3421 tctgcaggcc agaagttcaa tgtcagtttt atagagccaa aatctagttg tcactgggcc
     3481 aggacttggg aggctcctat agagtctgtc cctgcttcct cctgcagatt gttgtttggg
     3541 ttcatatcac ttcagtcctg ttgctttcac ttcgtcttct ctttctccaa atctcttctg
     3601 actgtccttt ataaggacac ttatgattgg atttgaggca tgtcggacac tacaggatga
     3661 tcttctcaca cctgcaagga atccttacaa aggaattgta catttcagag attagaaccg
     3721 gaggccccag agatgttgag tcaatgtatt acagtagtgt ttgctacttc accatttcaa
     3781 gggactgtgg atataaatga ttgttttgaa ggccataacg gatctataag ccactttaga
     3841 gctgggaaac taagctgccg tttgtacccc tgctcatctt ctctacattg tgttcaacaa
     3901 tgaaccttga agaaaataaa acccaggtct ccagccttcc ccagccccgt gcatgcgcat
     3961 tctccttcac ccccaattcc ttttgagtaa gttacttgtt aaaataaatc cttaaggttc
     4021 aaaacaaaaa aaaaaaaaaa
//
