LOCUS       NM_146206               2774 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus two pore segment channel 2 (Tpcn2), transcript variant
            1, mRNA.
ACCESSION   NM_146206
VERSION     NM_146206.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   de Zelicourt,A., Fayssoil,A., Mansart,A., Zarrouki,F., Karoui,A.,
            Piquereau,J., Lefebvre,F., Gerbaud,P., Mika,D.,
            Dakouane-Giudicelli,M., Lanchec,E., Feng,M., Leblais,V., Bobe,R.,
            Launay,J.M., Galione,A., Gomez,A.M., de la Porte,S. and
            Cancela,J.M.
  TITLE     Two-pore channels (TPCs) acts as a hub for excitation-contraction
            coupling, metabolism and cardiac hypertrophy signalling
  JOURNAL   Cell Calcium 117, 102839 (2024)
   PUBMED   38134531
REFERENCE   3  (bases 1 to 2774)
  AUTHORS   Goretzko,J., Pauels,I., Heitzig,N., Thomas,K., Kardell,M., Nass,J.,
            Krogsaeter,E.K., Schloer,S., Spix,B., Linard Matos,A.L., Leser,C.,
            Wegner,T., Glorius,F., Bracher,F., Gerke,V., Rossaint,J., Grimm,C.
            and Rescher,U.
  TITLE     P-selectin-dependent leukocyte adhesion is governed by
            endolysosomal two-pore channel 2
  JOURNAL   Cell Rep 42 (12), 113501 (2023)
   PUBMED   38039128
REFERENCE   4  (bases 1 to 2774)
  AUTHORS   Kounoupa,Z., Tivodar,S., Theodorakis,K., Kyriakis,D., Denaxa,M. and
            Karagogeos,D.
  TITLE     Rac1 and Rac3 GTPases and TPC2 are required for axonal outgrowth
            and migration of cortical interneurons
  JOURNAL   J Cell Sci 136 (6) (2023)
   PUBMED   36744839
  REMARK    GeneRIF: Rac1 and Rac3 GTPases and TPC2 are required for axonal
            outgrowth and migration of cortical interneurons.
REFERENCE   5  (bases 1 to 2774)
  AUTHORS   Wang,Q., Wang,Z., Wang,Y., Qi,Z., Bai,D., Wang,C., Chen,Y., Xu,W.,
            Zhu,X., Jeon,J., Xiong,J., Hao,C., Zhu,M.X., Wei,A. and Li,W.
  TITLE     A gain-of-function TPC2 variant R210C increases affinity to
            PI(3,5)P2 and causes lysosome acidification and hypopigmentation
  JOURNAL   Nat Commun 14 (1), 226 (2023)
   PUBMED   36641477
  REMARK    GeneRIF: A gain-of-function TPC2 variant R210C increases affinity
            to PI(3,5)P2 and causes lysosome acidification and
            hypopigmentation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2774)
  AUTHORS   Notomi,T., Ezura,Y. and Noda,M.
  TITLE     Identification of two-pore channel 2 as a novel regulator of
            osteoclastogenesis
  JOURNAL   J Biol Chem 287 (42), 35057-35064 (2012)
   PUBMED   22833668
  REMARK    GeneRIF: TPC2 is a novel critical molecule for osteoclastogenesis.
REFERENCE   7  (bases 1 to 2774)
  AUTHORS   Tugba Durlu-Kandilci,N., Ruas,M., Chuang,K.T., Brading,A.,
            Parrington,J. and Galione,A.
  TITLE     TPC2 proteins mediate nicotinic acid adenine dinucleotide phosphate
            (NAADP)- and agonist-evoked contractions of smooth muscle
  JOURNAL   J Biol Chem 285 (32), 24925-24932 (2010)
   PUBMED   20547763
  REMARK    GeneRIF: proteins mediate a key NAADP-regulated tissue response
            brought about by agonist activation of a cell surface recepto
REFERENCE   8  (bases 1 to 2774)
  AUTHORS   Schieder,M., Rotzer,K., Bruggemann,A., Biel,M. and Wahl-Schott,C.A.
  TITLE     Characterization of two-pore channel 2 (TPCN2)-mediated Ca2+
            currents in isolated lysosomes
  JOURNAL   J Biol Chem 285 (28), 21219-21222 (2010)
   PUBMED   20495006
  REMARK    GeneRIF: analysis of two-pore channel 2 (TPCN2)-mediated Ca2+
            currents in isolated lysosomes
REFERENCE   9  (bases 1 to 2774)
  AUTHORS   Zong,X., Schieder,M., Cuny,H., Fenske,S., Gruner,C., Rotzer,K.,
            Griesbeck,O., Harz,H., Biel,M. and Wahl-Schott,C.
  TITLE     The two-pore channel TPCN2 mediates NAADP-dependent Ca(2+)-release
            from lysosomal stores
  JOURNAL   Pflugers Arch 458 (5), 891-899 (2009)
   PUBMED   19557428
  REMARK    GeneRIF: TPCN2 is a major component of the long-sought lysosomal
            NAADP-dependent Ca(2+)-release channel.
REFERENCE   10 (bases 1 to 2774)
  AUTHORS   Calcraft,P.J., Ruas,M., Pan,Z., Cheng,X., Arredouani,A., Hao,X.,
            Tang,J., Rietdorf,K., Teboul,L., Chuang,K.T., Lin,P., Xiao,R.,
            Wang,C., Zhu,Y., Lin,Y., Wyatt,C.N., Parrington,J., Ma,J.,
            Evans,A.M., Galione,A. and Zhu,M.X.
  TITLE     NAADP mobilizes calcium from acidic organelles through two-pore
            channels
  JOURNAL   Nature 459 (7246), 596-600 (2009)
   PUBMED   19387438
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132081.3.
            
            On Jan 7, 2022 this sequence version replaced NM_146206.5.
            
            Transcript Variant: This variant (1) encodes the protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK052930.1, BC141195.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-77                AC132081.3         113689-113765       c
            78-142              AC132081.3         110067-110131       c
            143-219             AC132081.3         109115-109191       c
            220-397             AC132081.3         108575-108752       c
            398-514             AC132081.3         106947-107063       c
            515-621             AC132081.3         103700-103806       c
            622-697             AC132081.3         102118-102193       c
            698-797             AC132081.3         99572-99671         c
            798-863             AC132081.3         98698-98763         c
            864-928             AC132081.3         97906-97970         c
            929-1029            AC132081.3         97141-97241         c
            1030-1111           AC132081.3         96616-96697         c
            1112-1198           AC132081.3         96396-96482         c
            1199-1318           AC132081.3         91986-92105         c
            1319-1378           AC132081.3         91771-91830         c
            1379-1507           AC132081.3         90710-90838         c
            1508-1557           AC132081.3         89927-89976         c
            1558-1657           AC132081.3         88353-88452         c
            1658-1729           AC132081.3         87634-87705         c
            1730-1803           AC132081.3         87010-87083         c
            1804-1885           AC132081.3         86568-86649         c
            1886-1968           AC132081.3         86352-86434         c
            1969-2050           AC132081.3         85718-85799         c
            2051-2133           AC132081.3         85289-85371         c
            2134-2774           AC132081.3         83896-84536         c
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7"
     gene            1..2774
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /note="two pore segment channel 2"
                     /db_xref="GeneID:233979"
                     /db_xref="MGI:MGI:2385297"
     exon            1..77
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..2212
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /note="two pore calcium channel protein 2;
                     voltage-dependent calcium channel protein TPC2"
                     /codon_start=1
                     /product="two pore channel protein 2"
                     /protein_id="NP_666318.2"
                     /db_xref="CCDS:CCDS40203.1"
                     /db_xref="GeneID:233979"
                     /db_xref="MGI:MGI:2385297"
                     /translation="MAAEEQPLLGRDRGSGQVHSGAAADQELCIDQAVVFIEDAIKYR
                     SIYHRMDAGSLWLYRWYYSNVCQRVLGFIIFLILILAFVEVPSSFTKTADVRYRSQPW
                     QPPCGLTETIEAFCLLAFLVDLSVKGYLVGQAQLQQNLWLLAYFMVLVVSVVDWIVSL
                     SLACEEPLRMRRLLRPFFLLQNSSMMKKTLKCIRWSLPEMASVGLLLAIHLCLFTIIG
                     MLLFTIGEKDEAQDQERLAYFRNLPEALTSLLVLLTTSNNPDVMIPAYTQNRAFALFF
                     IVFTLIGSLFLMNLLTAIIYNQFRGYLMKSLQTSLFRRRLGARAAYEVLASRAGPAGT
                     TPELVGVNPETFLPVLQKTQLNKTHKQAIMQKVQSYEGRPMLADEFQKLFDEVDKGLA
                     KERPLKPQYQSPFLQTAQFIFSHHYFDYLGNLVALGNLLSICVFLVLDSDLLPGERDD
                     FVLGILDYIFILYYLLELLFKVFALGLPGYLSYHSNVFDGLLTIILLVSEICTLAVYR
                     LPHSGWKPEQYGPLSLWDMTRLMNTLIVFRFLRIIPNIKPMAEVANTILGLIPNLRAF
                     GGILVVAYYVFAMIGINLFRGVIVPPGNSSLVPDNNSAVCGSFEQLGYWPNNFDDFAA
                     ALITLWNVMVVNNWQVILEAYKRYAGPWSMVYFVLWWLVSSVIWINLFLALLLENFLH
                     RWDPQGHKQLLVGTKQMSVELMFRDILEEPKEEELMEKLHKHPHLHLCR"
     exon            78..142
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            143..219
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            220..397
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            398..514
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            515..621
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            622..697
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            698..797
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            798..863
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            864..928
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            929..1029
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1111
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1198
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1318
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1378
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1507
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1557
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            1558..1657
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1729
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            1730..1803
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            1804..1885
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..1968
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            1969..2050
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            2051..2133
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     exon            2134..2774
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2752..2757
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /note="hexamer: TATAAA"
     polyA_site      2774
                     /gene="Tpcn2"
                     /gene_synonym="D830047E22Rik; Gm35086"
                     /note="major polyA site"
ORIGIN      
        1 ctgttacatt ggtgggatgg cggcagaaga gcagcccctt ctgggccggg accgaggcag
       61 tggccaggtc cactccggtg ctgcagccga tcaggagctc tgcatagacc aggctgtggt
      121 cttcattgaa gacgccataa agtaccgctc catctaccac cgcatggatg ctggttcatt
      181 gtggctttac cgctggtatt actcgaacgt ctgccaacgt gtgctgggct tcatcatttt
      241 cctgatcctg atcttggctt ttgtggaggt cccatcttcc ttcacaaaga ctgcagatgt
      301 gcgctaccgt tcccagccct ggcagccgcc ctgtggcctg accgagacga tcgaggcgtt
      361 ctgcttgctt gccttcctgg ttgacctctc tgtgaagggc tacttggttg ggcaggccca
      421 gttgcagcag aacctgtggc tgctggccta ctttatggtg ctggttgtgt ctgtggtgga
      481 ctggatcgtt tcgctgagcc ttgcttgtga ggagcccctg cggatgcgcc ggctgctccg
      541 ccccttcttc ctgctgcaga attcctccat gatgaagaag accctgaagt gtatacggtg
      601 gtcgctacca gaaatggcca gtgtcgggct gctgctggcc atccacctgt gtctcttcac
      661 catcattggg atgctgctgt tcaccattgg tgagaaggat gaagcacagg accaggagag
      721 gctggcctac ttccggaacc ttccagaagc actgacctca ctcctagtgc tgctgacgac
      781 ttccaacaac cctgatgtga tgatccctgc gtatactcag aaccgggcct ttgctctctt
      841 cttcatagtc ttcaccttga taggaagctt gtttctgatg aacctgctga cagccatcat
      901 ctataatcag ttccgtggct acctaatgaa atctctacag acctcactgt tccggcggcg
      961 actgggggcc cgtgcagcct acgaagtcct cgcctccagg gccgggccag ctggaaccac
     1021 ccctgagtta gttggggtga atcctgagac cttcctgcca gtgcttcaga aaacgcagct
     1081 gaacaaaacc cacaaacaag ccattatgca gaaggtgcag tcctatgaag gccgcccaat
     1141 gctggctgat gagttccaga aactcttcga tgaggttgac aaaggtttgg ccaaagagcg
     1201 cccgctgaag ccccaatacc agtctccatt tctgcagact gcccagttca tctttagcca
     1261 ccactacttc gactacctgg ggaacctcgt cgctctggga aacctcttgt ctatttgtgt
     1321 gttcctggtg ctggactcag acctgctgcc tggggaacgt gatgactttg tcctggggat
     1381 tcttgactac atcttcatct tgtactacct gctggagctg ctgtttaagg tgttcgcgct
     1441 gggcctgcca ggctacctgt cctaccatag caatgtgttt gatggcctcc tcaccatcat
     1501 cctgctggtt tcggagattt gcaccctggc tgtataccga ttgccgcact caggatggaa
     1561 gccagagcag tatggcccac tgtcgctctg ggacatgaca cgactgatga acacactgat
     1621 tgtgtttcgc ttcctgcgca tcatccccaa cataaagcca atggctgagg tagccaacac
     1681 catcctgggc ctaatcccta acttgagggc atttggtggg atcctggtgg tggcatacta
     1741 tgtgtttgcc atgatcggga tcaacctgtt ccgaggtgtc atcgtgcctc ctggaaacag
     1801 cagcctggtt cccgacaaca actcagctgt atgtggaagc tttgagcagc taggttactg
     1861 gcccaacaac tttgacgact ttgctgctgc tttgatcaca ctgtggaacg tgatggtggt
     1921 gaacaattgg caggtgatcc tggaagccta taagcgctac gcaggcccgt ggtcaatggt
     1981 gtattttgtg ctatggtggc tggtgtcctc tgttatctgg atcaacctgt ttctggctct
     2041 tcttctggag aactttctcc acagatggga ccctcaaggt cataagcagc tccttgttgg
     2101 gaccaagcag atgtctgtgg agctcatgtt cagggatatc ctagaagagc ccaaggagga
     2161 ggaactgatg gagaagctac acaaacaccc acacttgcat ctgtgcaggt gacatcagag
     2221 tggcttcctt gctggggtga caggacagag atgctggcct gggcccatgc tgtggaggaa
     2281 gcaactggtc gaggcagagc aagaaaagat ggtgcctggg tcagaccact cagagacaga
     2341 cagaggctgg gacaaggact catcctctgt ggttgctcag cagtagccaa ggtgtagctt
     2401 tggcagacca ttagtcggca ccacagggcc acattcctcc atttgttgct cccccaagct
     2461 gtcttggtga gacttgctaa ggagagagag tgacttccaa gggtccttcc tcaaagccag
     2521 gaagcaagaa ggcctcagct ctccttgata cccacaaact tatctgtgcc ttcagcctca
     2581 gtggcatttg tgaacccaag cctgtacagg acagtaactg gcaggtctcc aggacattgg
     2641 ggttggtgtc atgtgtagag aatgtcacct ctttgtttca gtgctgtgga tgtgatgggg
     2701 gtgtcccggg ggtggggagg gtgaggtcac aatgtcaatc attttctgtt ttataaagaa
     2761 tgagatcatt ctta
//
