LOCUS       NM_001033447            2262 bp    mRNA    linear   ROD 26-SEP-2023
DEFINITION  Mus musculus TOP6B like initiator of meiotic double strand breaks
            (Top6bl), mRNA.
ACCESSION   NM_001033447 XM_355126 XM_905554
VERSION     NM_001033447.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Nore A, Juarez-Martinez AB, Clement J, Brun C, Diagouraga B,
            Laroussi H, Grey C, Bourbon HM, Kadlec J, Robert T and de Massy B.
  TITLE     TOPOVIBL-REC114 interaction regulates meiotic DNA double-strand
            breaks
  JOURNAL   Nat Commun 13 (1), 7048 (2022)
   PUBMED   36396648
  REMARK    GeneRIF: TOPOVIBL-REC114 interaction regulates meiotic DNA
            double-strand breaks.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Nguyen NMP, Ge ZJ, Reddy R, Fahiminiya S, Sauthier P, Bagga R,
            Sahin FI, Mahadevan S, Osmond M, Breguet M, Rahimi K, Lapensee L,
            Hovanes K, Srinivasan R, Van den Veyver IB, Sahoo T, Ao A, Majewski
            J, Taketo T and Slim R.
  TITLE     Causative Mutations and Mechanism of Androgenetic Hydatidiform
            Moles
  JOURNAL   Am J Hum Genet 103 (5), 740-751 (2018)
   PUBMED   30388401
REFERENCE   3  (bases 1 to 2262)
  AUTHORS   Robert T, Nore A, Brun C, Maffre C, Crimi B, Bourbon HM and de
            Massy B.
  TITLE     The TopoVIB-Like protein family is required for meiotic DNA
            double-strand break formation
  JOURNAL   Science 351 (6276), 943-949 (2016)
   PUBMED   26917764
  REMARK    Erratum:[Science. 2016 May 6;352(6286). pii: aaf9649. doi:
            10.1126/science.aaf9649. PMID: 27151875]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC147618.3 and AC128739.4.
            
            [WARNING] On Jan 9, 2024 this sequence was replaced by
            NM_001033447.4.
            
            On Oct 21, 2011 this sequence version replaced NM_001033447.2.
            
            Sequence Note: The RefSeq transcript and protein were inferred by
            alignment of the human ortholog, NM_024650.3, because no single
            transcript from the same strain was available for the full length
            of the gene. The extent of this transcript is supported by
            transcript alignments and orthologous data.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164131
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-492               AC147618.3         96601-97092
            493-631             AC147618.3         99127-99265
            632-685             AC128739.4         2672-2725
            686-795             AC128739.4         23010-23119
            796-879             AC128739.4         31562-31645
            880-935             AC128739.4         34888-34943
            936-1007            AC128739.4         35679-35750
            1008-1203           AC128739.4         36722-36917
            1204-1354           AC128739.4         42902-43052
            1355-1501           AC128739.4         45358-45504
            1502-1623           AC128739.4         49456-49577
            1624-1734           AC128739.4         52954-53064
            1735-1833           AC128739.4         57861-57959
            1834-1912           AC128739.4         67930-68008
            1913-2262           AC128739.4         68987-69336
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 4.08 cM"
     gene            1..2262
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /note="TOP6B like initiator of meiotic double strand
                     breaks"
                     /db_xref="GeneID:381196"
                     /db_xref="MGI:MGI:2685806"
     CDS             1..2262
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /note="type 2 DNA topoisomerase VI subunit B-like"
                     /codon_start=1
                     /product="type 2 DNA topoisomerase 6 subunit B-like"
                     /protein_id="NP_001028619.2"
                     /db_xref="CCDS:CCDS57129.1"
                     /db_xref="GeneID:381196"
                     /db_xref="MGI:MGI:2685806"
                     /translation="MWEVEPGPRHRPQNRRQSHLGSRASLGILHYAAAAFQGAALENA
                     EGGQSALKRAEGRVLARPWESLRSSRRPASSRLASNLGRCATCPPAHPPTDAGRLGQL
                     PQCAGARAGGGADARSGGKGLPGVGALRVEALAEEEAVTNWQSQSCSRSPYRMERTAL
                     AVCEILRYLIIHWKCEAGTAKGTLLDGQLVISIEALRSKHLPDSLHCIITIASTRSVY
                     GGLNFKKFLQEIQPALPRLSAKLALASEEGGRSQDASGIAPCQVTFEVDENSQSLMTD
                     CLVIKHFLRKIIIVHHKLKFSFSVAVNGTLSAETFGAENEPTLRLDNGVTLVVGFQRY
                     VSKPKLNWSEAHCSRIHPVLGHPAPLFIPDAKADTGLLGELTLTPAAALCPSPKGFSS
                     QLCRISSVSIFLYGPLGLPLLSSDQDQPSTAVFRDTSYFIDWKKYNLFMVPNLDLNLD
                     TQSVLPDVNYKAESPEGNQSQNMNAQGPALLLFLFVDFQSDVPVQQAKIWGLHTLLTA
                     HLSAILSESRSTVQQSIQSAVDQVWQLYHHDAKTQQRLQASLSVAVNSIMSVLTGSTR
                     SSFRKTCLQALEAADTQEFGVKLHRIFYDITQHQFLKHCSCDTEQHLTPEKNISAQNT
                     KDQHKNIAQEFPEESIGQAENKRPKRGSPNHGREESRVLGSARDRSPPKSATRDRELT
                     EVSLTARGSQTQAAHGRAQAAEAASPAGGLEVPQREQGGCGPELPPPGPHHRLLCSRR
                     TCGYRRYPICPSG"
     exon            1..492
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
     exon            493..631
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
     exon            632..685
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
     exon            686..795
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
     exon            796..879
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
     exon            880..935
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1007
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1203
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..1354
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1501
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1623
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1734
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1833
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1912
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..2262
                     /gene="Top6bl"
                     /gene_synonym="Gm960; TOPOVIBL"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgtgggaag tggagcctgg tcccaggcac cgcccgcaga acagaaggca aagccatctg
       61 gggtctagag ccagcttggg aatcctgcac tacgcagccg cggctttcca gggagcagcc
      121 ctggaaaacg ccgagggcgg acagagcgct ttgaaaaggg ccgagggccg tgtcttggcg
      181 agaccgtggg aaagtctcag gagcagtcgc cgcccggcct cttcccggct cgcctccaac
      241 cttggcagat gcgccacctg tcctcccgcc cacccgccta cggatgcggg ccgcctgggg
      301 cagctcccgc agtgcgcagg cgcgagggct gggggcgggg cggacgcgcg gtcaggcggg
      361 aagggactgc ccggcgtggg cgccctccgg gtcgaagccc tagctgagga ggaggctgtg
      421 accaactggc agtcccagag ctgcagccgc tctccttaca gaatggagag gacggctttg
      481 gctgtctgtg agattttaag atatttaata attcactgga aatgtgaggc aggaacagca
      541 aagggaacat tgctggatgg gcagctagtg atttccatag aagcgttacg ttctaagcac
      601 ctgccagatt ctctgcattg tataataact attgcttcta ccagaagtgt ttatggtggc
      661 ttgaacttca agaagtttct acaagaaata cagcctgcgc tacccaggct ctctgcaaag
      721 ctggctctgg cttcagagga ggggggccgt tctcaagacg catcaggcat agcaccctgc
      781 caggtgactt ttgaggttga tgaaaattct caaagtttga tgacagactg tctggttata
      841 aagcactttt tacgaaaaat catcatagtt catcacaagc tcaagttttc tttcagtgtc
      901 gcggtgaatg ggaccctctc tgcagagacc ttcggggcag agaatgaacc tactttgaga
      961 ctggacaatg gagtcactct tgttgtgggc ttccagcgct atgtgagtaa accaaagttg
     1021 aattggagtg aagcacactg cagcagaatc caccctgtgc tagggcatcc ggccccactg
     1081 ttcatccccg atgccaaggc cgacacgggc ctgctgggag aactgacact gactccggct
     1141 gctgctctgt gccccagccc caagggcttc tccagccaac tgtgcaggat ttcttcagtt
     1201 tccatatttc tatatggacc tttgggtctg cctctgctat catcagatca ggatcagcca
     1261 agtacagctg tcttcagaga tacctcttat ttcattgact ggaagaaata caatttgttt
     1321 atggtgccca atttggatct caatttggat acacagtctg tgcttccaga tgtgaattat
     1381 aaagcagagt cgcctgaggg gaatcagtct cagaatatga acgcacaggg accagctctg
     1441 ctacttttcc tatttgtgga tttccaaagt gacgtcccag tacaacaagc gaaaatctgg
     1501 ggactccaca ctctgctcac agcccatctc agcgccatcc tctcggagag ccgcagcacg
     1561 gtacagcagt ccatccagtc agctgtggat caggtctggc agctgtacca ccatgatgcc
     1621 aagactcaac agagactgca ggcctccctc tctgtggctg tgaactccat catgagtgtg
     1681 ttgactggaa gcacccgcag cagcttcagg aagacatgcc tccaggctct tgaggcagct
     1741 gacacacagg aatttggtgt caaactgcac agaatatttt atgatatcac ccagcaccaa
     1801 tttctgaagc attgctcatg tgacacagag cagcacctga ctccagagaa aaacatttca
     1861 gcccagaaca ccaaagacca gcacaagaat attgcccagg aattccctga agaatccatc
     1921 gggcaagcag aaaacaagag gcccaagagg ggcagcccca accatggcag ggaagaatcg
     1981 cgagttctgg gctcagcaag agaccggagc ccgccaaaat ccgccacgcg cgaccgcgag
     2041 ctcaccgagg tctccctcac tgctagagga tcgcagaccc aagcggccca cggcagggcc
     2101 caggcggcag aggcagctag ccctgcgggc ggcctggagg taccgcagcg agagcagggc
     2161 gggtgcgggc ctgagctacc accgccgggt cctcaccacc gcctcctctg ctcccgcagg
     2221 acttgtggct acaggaggta tccaatctgt ccgagtggct ga
//
