LOCUS       NP_035754                862 aa            linear   ROD 11-MAR-2025
DEFINITION  DNA topoisomerase 3-beta-1 [Mus musculus].
ACCESSION   NP_035754
VERSION     NP_035754.1
DBSOURCE    REFSEQ: accession NM_011624.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 862)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 862)
  AUTHORS   Zhu,X., Joo,Y., Bossi,S., McDevitt,R.A., Xie,A., Wang,Y., Xue,Y.,
            Su,S., Lee,S.K., Sah,N., Zhang,S., Ye,R., Pinto,A., Zhang,Y.,
            Araki,K., Araki,M., Morales,M., Mattson,M.P., van Praag,H. and
            Wang,W.
  TITLE     Tdrd3-null mice show post-transcriptional and behavioral
            impairments associated with neurogenesis and synaptic plasticity
  JOURNAL   Prog Neurobiol 233, 102568 (2024)
   PUBMED   38216113
REFERENCE   3  (residues 1 to 862)
  AUTHORS   Cuarenta,A., Kigar,S.L., Keese,A.P., Guagliardo,S.E., Chang,L.,
            Bakshi,V.P. and Auger,A.P.
  TITLE     DNA topoisomerase Top3beta is impacted by early life stress in the
            developing female and male rat brain
  JOURNAL   Brain Res 1809, 148339 (2023)
   PUBMED   36966960
  REMARK    GeneRIF: DNA topoisomerase Top3beta is impacted by early life
            stress in the developing female and male rat brain.
REFERENCE   4  (residues 1 to 862)
  AUTHORS   Rahman,F.U., Kim,Y.R., Kim,E.K., Kim,H.R., Cho,S.M., Lee,C.S.,
            Kim,S.J., Araki,K., Yamamura,K.I., Lee,M.N., Park,S.G., Yoon,W.K.,
            Lee,K., Won,Y.S., Kim,H.C., Lee,Y., Lee,H.Y. and Nam,K.H.
  TITLE     Topoisomerase IIIbeta Deficiency Induces Neuro-Behavioral Changes
            and Brain Connectivity Alterations in Mice
  JOURNAL   Int J Mol Sci 22 (23), 12806 (2021)
   PUBMED   34884616
  REMARK    GeneRIF: Topoisomerase IIIbeta Deficiency Induces Neuro-Behavioral
            Changes and Brain Connectivity Alterations in Mice.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 862)
  AUTHORS   Joo,Y., Xue,Y., Wang,Y., McDevitt,R.A., Sah,N., Bossi,S., Su,S.,
            Lee,S.K., Peng,W., Xie,A., Zhang,Y., Ding,Y., Ku,W.L., Ghosh,S.,
            Fishbein,K., Shen,W., Spencer,R., Becker,K., Zhao,K., Mattson,M.P.,
            van Praag,H., Sharov,A. and Wang,W.
  TITLE     Topoisomerase 3beta knockout mice show transcriptional and
            behavioural impairments associated with neurogenesis and synaptic
            plasticity
  JOURNAL   Nat Commun 11 (1), 3143 (2020)
   PUBMED   32561719
  REMARK    GeneRIF: Topoisomerase 3beta knockout mice show transcriptional and
            behavioural impairments associated with neurogenesis and synaptic
            plasticity.
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 862)
  AUTHORS   Kwan,K.Y., Moens,P.B. and Wang,J.C.
  TITLE     Infertility and aneuploidy in mice lacking a type IA DNA
            topoisomerase III beta
  JOURNAL   Proc Natl Acad Sci U S A 100 (5), 2526-2531 (2003)
   PUBMED   12591952
  REMARK    GeneRIF: DNA topoisomerase III beta has a role in infertility and
            aneuploidy
REFERENCE   7  (residues 1 to 862)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (residues 1 to 862)
  AUTHORS   Kwan,K.Y. and Wang,J.C.
  TITLE     Mice lacking DNA topoisomerase IIIbeta develop to maturity but show
            a reduced mean lifespan
  JOURNAL   Proc Natl Acad Sci U S A 98 (10), 5717-5721 (2001)
   PUBMED   11331780
REFERENCE   9  (residues 1 to 862)
  AUTHORS   Wilson,T.M., Chen,A.D. and Hsieh,T.
  TITLE     Cloning and characterization of Drosophila topoisomerase IIIbeta.
            Relaxation of hypernegatively supercoiled DNA
  JOURNAL   J Biol Chem 275 (3), 1533-1540 (2000)
   PUBMED   10636841
REFERENCE   10 (residues 1 to 862)
  AUTHORS   Seki,T., Seki,M., Onodera,R., Katada,T. and Enomoto,T.
  TITLE     Cloning of cDNA encoding a novel mouse DNA topoisomerase III (Topo
            IIIbeta) possessing negatively supercoiled DNA relaxing activity,
            whose message is highly expressed in the testis
  JOURNAL   J Biol Chem 273 (44), 28553-28556 (1998)
   PUBMED   9786843
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166832.2, BY035336.1, AK146475.1 and AW122701.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031723.1, AK146475.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 10.46 cM"
     Protein         1..862
                     /product="DNA topoisomerase 3-beta-1"
                     /EC_number="5.6.2.1"
                     /note="DNA topoisomerase III beta-1; Topo III beta"
                     /calculated_mol_wt=96819
     Region          3..167
                     /region_name="TOPRIM_TopoIA_TopoIII"
                     /note="The topoisomerase-primase (TORPIM) domain found in
                     members of the type IA family of DNA topoisomerases (Topo
                     IA) similar to topoisomerase III. Type IA DNA
                     topoisomerases remove (relax) negative supercoils in the
                     DNA by: cleaving one strand of the DNA...; cd03362"
                     /db_xref="CDD:173782"
     Site            order(9..10,13,117,119,121)
                     /site_type="active"
                     /db_xref="CDD:173782"
     Site            order(16,118,120,127..128,130..131)
                     /site_type="other"
                     /note="putative interdomain interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:173782"
     Site            order(117,119)
                     /site_type="other"
                     /note="putative metal-binding site [ion binding]"
                     /db_xref="CDD:173782"
     Site            120
                     /site_type="other"
                     /note="putative nucleotide binding site [chemical
                     binding]"
                     /db_xref="CDD:173782"
     Region          172..593
                     /region_name="TOP1Ac"
                     /note="DNA Topoisomerase, subtype IA; DNA-binding,
                     ATP-binding and catalytic domain of bacterial DNA
                     topoisomerases I and III, and eukaryotic DNA topoisomerase
                     III and eubacterial and archael reverse gyrases.
                     Topoisomerases clevage single or double stranded DNA...;
                     cd00186"
                     /db_xref="CDD:238110"
     Site            order(172..199,212..232)
                     /site_type="active"
                     /note="domain I [active]"
                     /db_xref="CDD:238110"
     Site            order(181..182,185,189,193,326,330,338,512..513,516..517,
                     519,523..524)
                     /site_type="DNA binding"
                     /note="DNA binding groove [nucleotide binding]"
                     /db_xref="CDD:238110"
     Site            order(223,227,549)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:238110"
     Site            order(237..244,282..296,422..436,438..456,479..489)
                     /site_type="active"
                     /note="domain II [active]"
                     /db_xref="CDD:238110"
     Site            order(297..303,305..360,386..394,401..421)
                     /site_type="active"
                     /note="domain III [active]"
                     /db_xref="CDD:238110"
     Site            order(308,312..314,387,407,410,414,510,512,535)
                     /site_type="other"
                     /note="nucleotide binding site [chemical binding]"
                     /db_xref="CDD:238110"
     Site            order(336,338,387)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238110"
     Site            order(490..530,535..549,555..593)
                     /site_type="active"
                     /note="domain IV [active]"
                     /db_xref="CDD:238110"
     Region          820..855
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z321.1)"
     CDS             1..862
                     /gene="Top3b"
                     /coded_by="NM_011624.3:436..3024"
                     /db_xref="CCDS:CCDS27990.1"
                     /db_xref="GeneID:21976"
                     /db_xref="MGI:MGI:1333803"
ORIGIN      
        1 mktvlmvaek pslaqsiaki lsrgnmsshk glngacsvhk ytgtfagqpv hfkmtsvcgh
       61 vmtldflgky nkwdkvdpae lfsqaptekk eanpklnmvk flqvegrgcd yvvlwldcdk
      121 egenicfevl davlpvmnna hngektvfra rfssitdtdi cnamtrlsep dhnealsvda
      181 rqeldlrigc aftrfqtkyf qgkygdldss lisfgpcqtp tlgfcverhd kiqsfkpety
      241 wvlqakvhtd keesllldwd rvrvfdweia qmflnmtkle keawveatsr kekakqrpla
      301 lntvemlrva ssalgmgpqh amqiaerlyt qgyisyprte tthypenfdl kgslrqqanh
      361 pywadsvkql laeginrprk ghdagdhppi tpmksateae lggdawrlye yitrhfiatv
      421 shdckylqst isfrigpehf tcmgktvisp gfteimpwqs vpleeslptc qkgdtftvge
      481 vkmlekqtsp pdylteaeli tlmekhgigt dasipvhinn icqrnyvtve sgrrlkptnl
      541 givlvhgyyk idaelvlpti rsavekqlnl iaqgkadyhq vlghtldifk rkfhyfvdsi
      601 agmdelmevs fsplaatgkp lsrcgkchrf mkyiqakpsr lhcshcdety tlpqngtikl
      661 ykelrcpldd felvlwssgs rgksyplcpy cynhppfrdm kkgmgcnect hptcqhslsm
      721 lgigqcvece ngvlvldpts gpkwkvacnt cnvvahcfen ahrvrvsadt cntceaalld
      781 vdfnkakspl pgnetqhtgc ifcdpvfqel velkhaasch pmhrggpgrr qgrgrgrgrr
      841 ppgkpnprrp kdkmsalaay fv
//
