LOCUS       NM_009409               5661 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus topoisomerase (DNA) II beta (Top2b), mRNA.
ACCESSION   NM_009409
VERSION     NM_009409.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Segev,A., Heady,L., Crewe,M. and Madabhushi,R.
  TITLE     Mapping catalytically engaged TOP2B in neurons reveals the
            principles of topoisomerase action within the genome
  JOURNAL   Cell Rep 43 (2), 113809 (2024)
   PUBMED   38377005
  REMARK    GeneRIF: Mapping catalytically engaged TOP2B in neurons reveals the
            principles of topoisomerase action within the genome.
REFERENCE   3  (bases 1 to 5661)
  AUTHORS   Zhang,W., Zhang,R., Wu,L., Zhu,C., Zhang,C., Xu,C., Zhao,S.,
            Liu,X., Guo,T., Lu,Y., Gao,Z., Yu,X., Li,L., Chen,Z.J., Qin,Y. and
            Jiao,X.
  TITLE     NLRP14 deficiency causes female infertility with oocyte maturation
            defects and early embryonic arrest by impairing cytoplasmic UHRF1
            abundance
  JOURNAL   Cell Rep 42 (12), 113531 (2023)
   PUBMED   38060382
REFERENCE   4  (bases 1 to 5661)
  AUTHORS   Mucino-Hernandez,G., Acevo-Rodriguez,P.S., Cabrera-Benitez,S.,
            Guerrero,A.O., Merchant-Larios,H. and Castro-Obregon,S.
  TITLE     Nucleophagy contributes to genome stability through degradation of
            type II topoisomerases A and B and nucleolar components
  JOURNAL   J Cell Sci 136 (1) (2023)
   PUBMED   36633090
  REMARK    GeneRIF: Nucleophagy contributes to genome stability through
            degradation of type II topoisomerases A and B and nucleolar
            components.
REFERENCE   5  (bases 1 to 5661)
  AUTHORS   Fulton,S.L., Wenderski,W., Lepack,A.E., Eagle,A.L., Fanutza,T.,
            Bastle,R.M., Ramakrishnan,A., Hays,E.C., Neal,A., Bendl,J.,
            Farrelly,L.A., Al-Kachak,A., Lyu,Y., Cetin,B., Chan,J.C.,
            Tran,T.N., Neve,R.L., Roper,R.J., Brennand,K.J., Roussos,P.,
            Schimenti,J.C., Friedman,A.K., Shen,L., Blitzer,R.D., Robison,A.J.,
            Crabtree,G.R. and Maze,I.
  TITLE     Rescue of deficits by Brwd1 copy number restoration in the Ts65Dn
            mouse model of Down syndrome
  JOURNAL   Nat Commun 13 (1), 6384 (2022)
   PUBMED   36289231
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5661)
  AUTHORS   Adachi,N., Miyaike,M., Kato,S., Kanamaru,R., Koyama,H. and
            Kikuchi,A.
  TITLE     Cellular distribution of mammalian DNA topoisomerase II is
            determined by its catalytically dispensable C-terminal domain
  JOURNAL   Nucleic Acids Res 25 (15), 3135-3142 (1997)
   PUBMED   9224616
REFERENCE   7  (bases 1 to 5661)
  AUTHORS   Juenke,J.M. and Holden,J.A.
  TITLE     The distribution of DNA topoisomerase II isoforms in differentiated
            adult mouse tissues
  JOURNAL   Biochim Biophys Acta 1216 (2), 191-196 (1993)
   PUBMED   8241259
REFERENCE   8  (bases 1 to 5661)
  AUTHORS   Adachi,N., Miyaike,M., Ikeda,H. and Kikuchi,A.
  TITLE     Characterization of cDNA encoding the mouse DNA topoisomerase II
            that can complement the budding yeast top2 mutation
  JOURNAL   Nucleic Acids Res 20 (20), 5297-5303 (1992)
   PUBMED   1331984
REFERENCE   9  (bases 1 to 5661)
  AUTHORS   Capranico,G., Tinelli,S., Austin,C.A., Fisher,M.L. and Zunino,F.
  TITLE     Different patterns of gene expression of topoisomerase II isoforms
            in differentiated tissues during murine development
  JOURNAL   Biochim Biophys Acta 1132 (1), 43-48 (1992)
   PUBMED   1380833
REFERENCE   10 (bases 1 to 5661)
  AUTHORS   Deffie,A.M., Bosman,D.J. and Goldenberg,G.J.
  TITLE     Evidence for a mutant allele of the gene for DNA topoisomerase II
            in adriamycin-resistant P388 murine leukemia cells
  JOURNAL   Cancer Res 49 (24 Pt 1), 6879-6882 (1989)
   PUBMED   2555055
  REMARK    Erratum:[Cancer Res 1990 Jan 15;50(2):449]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154452.2 and AC154471.2.
            
            On Sep 14, 2022 this sequence version replaced NM_009409.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC054541.1, D38046.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-549               AC154452.2         75705-76253
            550-705             AC154452.2         93589-93744
            706-796             AC154452.2         94984-95074
            797-860             AC154452.2         96618-96681
            861-1006            AC154452.2         97800-97945
            1007-1104           AC154452.2         98917-99014
            1105-1317           AC154452.2         100294-100506
            1318-1491           AC154452.2         103686-103859
            1492-1593           AC154452.2         105934-106035
            1594-1731           AC154452.2         106119-106256
            1732-1870           AC154452.2         108310-108448
            1871-2028           AC154452.2         109134-109291
            2029-2154           AC154452.2         109436-109561
            2155-2265           AC154452.2         110544-110654
            2266-2371           AC154452.2         110918-111023
            2372-2481           AC154452.2         117257-117366
            2482-2574           AC154452.2         117639-117731
            2575-2689           AC154452.2         117818-117932
            2690-2811           AC154452.2         119354-119475
            2812-2960           AC154452.2         119696-119844
            2961-3192           AC154452.2         120321-120552
            3193-3327           AC154452.2         122583-122717
            3328-3528           AC154452.2         123446-123646
            3529-3724           AC154452.2         124088-124283
            3725-3816           AC154452.2         124429-124520
            3817-3972           AC154452.2         127084-127239
            3973-4056           AC154452.2         127479-127562
            4057-4251           AC154452.2         128864-129058
            4252-4396           AC154452.2         130868-131012
            4397-4558           AC154452.2         131230-131391
            4559-4760           AC154452.2         133149-133350
            4761-4840           AC154452.2         134226-134305
            4841-4951           AC154471.2         20369-20479
            4952-5077           AC154471.2         23488-23613
            5078-5172           AC154471.2         24352-24446
            5173-5661           AC154471.2         25313-25801
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 7.08 cM"
     gene            1..5661
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /note="topoisomerase (DNA) II beta"
                     /db_xref="GeneID:21974"
                     /db_xref="MGI:MGI:98791"
     exon            1..549
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             502..5340
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /EC_number="5.6.2.2"
                     /note="DNA topoisomerase II, beta isozyme"
                     /codon_start=1
                     /product="DNA topoisomerase 2-beta"
                     /protein_id="NP_033435.2"
                     /db_xref="CCDS:CCDS26833.1"
                     /db_xref="GeneID:21974"
                     /db_xref="MGI:MGI:98791"
                     /translation="MAKSSLAGSDGALTWVNNATKKEELETANKNDSTKKLSVERVYQ
                     KKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAA
                     DNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNY
                     DDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHF
                     DGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGF
                     RSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRH
                     VDYVVDQVVSKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTL
                     QPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKL
                     DDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHK
                     QIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFI
                     HHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKG
                     LGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRR
                     QRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLF
                     TCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQ
                     FGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPM
                     VLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQN
                     QYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTD
                     TTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKE
                     FFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQ
                     RGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEK
                     VEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQEREDILAGMSGKAI
                     KGKVGKPKVKKLQLEETMPSPYGRRIVPEITAMKADASRKLLKKKKGDPDTTVVKVEF
                     DEEFSGTPAEGTGEETLTPSAPVNKGPKPKREKKEPGTRVRKTPTSTGKTNAKKVKKR
                     NPWSDDESKSESDLEEAEPVVIPRDSLLRRAAAERPKYTFDFSEEEDDDAAAADDSND
                     LEELKVKASPITNDGEDEFVPSDGLDKDEYAFSSGKSKATPEKSSNDKKSQDFGNLFS
                     FPSYSQKSEDDSAKFDSNEEDTASVFAPSFGLKQTDKLPSKTVAAKKGKPPSDTAPKA
                     KRAPKQKKIVETINSDSDSEFGIPKKTTTPKGKGRGAKKRKASGSENEGDYNPGRKPS
                     KTASKKPKKTSFDQDSDVDIFPSDFTSEPPALPRTGRARKEVKYFAESDEEEDVDFAM
                     FN"
     exon            550..705
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            706..796
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            797..860
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            861..1006
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1104
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1317
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1491
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1492..1593
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1594..1731
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1732..1870
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1871..2028
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2154
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2155..2265
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2266..2371
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2372..2481
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2482..2574
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2575..2689
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2690..2811
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2812..2960
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2961..3192
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3193..3327
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3328..3528
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3529..3724
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3725..3816
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3817..3972
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3973..4056
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4057..4251
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4252..4396
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4397..4558
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4559..4760
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4761..4840
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4841..4951
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4952..5077
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5078..5172
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5173..5661
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5644..5649
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /note="hexamer: ATTAAA"
     polyA_site      5661
                     /gene="Top2b"
                     /gene_synonym="D230016L12Rik; Top-2"
                     /note="major polyA site"
ORIGIN      
        1 gtgtgtgccg tgttatgccg gactaaaggg aggccaccac ggcggctctg tttattgtcc
       61 ctcttggact gtgctccgga ggaccggttc agacgccgct tgggcggttt tgaggcagtg
      121 gcggcctttg aggctgcggc gaccgcggcg aggactctgc ggcttcggtg gcttgaggag
      181 aagcagcggc tcattcctgt cagggctctg gctcgcgagt cctagccggc ccgcttcaac
      241 ctcgtccctc atccgcgatc ccaatcgccc gcgtgggtcc cggaaggccg cgccgcatcg
      301 ccagcccttc ctagtcctcg accactctgt aggcccgggc gccgcgctcg agtttgaggg
      361 cggcgggcag cgcggcctcc tcagcggctc gggcccgcga tggggcgcgg ctaggagtgc
      421 tgcgcgcagc ggtcagtgcg agcggcgggc ccggcgtccc cggagcgcga gcagcccctg
      481 gcctgcacgg gaggcgctgc catggccaag tccagcctcg cgggctctga cggggcgctg
      541 acctgggtga acaatgctac aaaaaaggaa gagttggaga cagcaaacaa aaatgactct
      601 actaagaagt tgtctgttga gagagtgtac cagaagaaga cccaactgga gcacattctc
      661 cttcgtcctg atacatacat tggatcagtg gagccactga cacagctaat gtgggtgtat
      721 gatgaagatg tagggatgaa ctgcagggag gtcacctttg ttccaggctt atacaagatc
      781 tttgatgaaa ttttggttaa tgctgctgac aacaagcaga gggacaagaa catgacctgt
      841 attaaggttt ctattgatcc tgaatctaat attataagca tttggaataa tgggaaaggc
      901 attccagtag tagaacacaa agtagagaaa gtttatgttc ctgctttaat ttttggacag
      961 cttttaacat cgagtaatta tgacgatgat gagaaaaaag ttacaggagg ccgtaatggt
     1021 tatggtgcaa agctttgtaa tatttttagt acaaagttta cagtagaaac ggcttgcaaa
     1081 gaatacaaac atagttttaa acagacatgg atgaataata tgatgaagac ttctgaagcc
     1141 aaaattaaac attttgatgg tgaagattat acatgcataa cattccagcc agatctatcc
     1201 aaatttaaga tggaaaaact tgacaaggac attgtggccc tcatgaccag aagagcatat
     1261 gatttagctg ggtcctgtaa aggagtcaag gtcatgttta atgggaagaa gttgcctgta
     1321 aatggatttc gcagctatgt agacctttat gtgaaagaca aattggatga aactggggtg
     1381 gctctcaaag ttattcatga acttgcaaat gaaagatggg atgtttgtct aaccttgagt
     1441 gaaaaaggtt tccagcagat cagctttgtg aatagtattg caactacaaa aggtgggcgg
     1501 catgtggatt atgtagtaga tcaagttgtt agtaaactga ttgaagtagt taagaaaaag
     1561 aacaaagctg gtgtatcagt gaaaccattt caggttaaaa accatatatg ggtttttatt
     1621 aattgtctta ttgaaaatcc aacctttgat tctcaaacta aggaaaacat gactctgcag
     1681 cccaaaagtt ttgggtccaa atgccaactg tcagaaaaat tttttaaagc agcctctaat
     1741 tgtggcattg tagaaagcat attgaactgg gtgaaattta aggctcagac tcaactgaat
     1801 aagaagtgtt catcagtaaa gtacagtaaa atcaaaggca ttcccaaact ggatgatgcc
     1861 aatgatgctg gtggcaaaca ttccctggag tgtacattaa tattaacaga gggggactcc
     1921 gccaaatctc tagctgtatc tggattaggt gtgattggta gggacagata tggagtcttc
     1981 ccactcaggg gtaaaattct caatgtacga gaagcctctc ataaacagat tatggaaaat
     2041 gcagagataa ataatattat taaaatagtt ggtttgcaat acaagaaaag ttatgatgat
     2101 gcagaatctc tgaaaacttt acgttatgga aagattatga ttatgactga tcaggatcaa
     2161 gatggttctc acatcaaagg cctgctaatc aattttattc atcacaattg gccatcactt
     2221 ttgaagcatg gttttcttga agaattcatt actcccattg taaaggcaag caaaaataag
     2281 caggaacttt ccttttatag tattcctgaa tttgatgagt ggaaaaaaca tatagaaaac
     2341 cagaaagcct ggaaaataaa gtactacaaa ggattaggta ccagtaccgc taaagaagca
     2401 aaggaatatt ttgctgatat ggaaaggcat cgcattttgt ttagatatgc tggacctgaa
     2461 gatgatgctg ccatcacctt ggcatttagt aagaagaaga ttgatgacag aaaagagtgg
     2521 ttaaccaact ttatggagga tcggagacag cgtaggttac atggcctacc agagcaattt
     2581 ttatatggta cagcaacaaa gcatttgaca tacaatgatt tcatcaacaa ggaattgatt
     2641 ctcttctcaa actcggacaa tgagagatct atcccatctc ttgtggatgg cttcaagcca
     2701 ggtcaacgaa aagttttgtt tacctgtttc aagaggaatg ataaacgtga agtgaaagta
     2761 gcgcagctgg ctggatctgt tgctgagatg tctgcttacc accatggaga acaagcattg
     2821 atgatgacta ttgtgaattt ggctcagaat tttgtgggca gtaataacat taacttgctt
     2881 cagcccattg gtcagtttgg aactcggctt catggtggca aagatgctgc aagccctcgt
     2941 tatattttca caatgttgag ctctctggca agacttcttt ttccagctgt ggatgataac
     3001 ttgcttaagt tcctttatga tgataatcaa cgtgtagagc cagagtggta tatccctata
     3061 atccccatgg ttttgattaa tggtgctgag ggtattggga ctggatgggc ttgtaaactg
     3121 cccaactatg atgctagaga aattgtgaac aatgtcaggc ggatgctaga aggtttggat
     3181 cctcatccca tgcttccaaa ctataagaac ttcaaaggca ccatccagga acttggtcag
     3241 aaccagtatg ctgttagtgg tgagatattt gtggtggaca gaaacacagt agagattaca
     3301 gaacttccag ttagaacatg gacacaggta tacaaggagc aggtcctaga acctatgctt
     3361 aatggaacag ataaaacacc ggcactgatt tctgattaca aagaatatca tactgacaca
     3421 actgtgaagt ttgtggtgaa aatgacagaa gagaaactag cgcaagcaga agctgctggc
     3481 ctgcataaag tttttaaact tcaaactact cttacttgca attccatggt actctttgat
     3541 catatgggtt gtctgaagaa gtatgaaact gtgcaagaca ttttgaaaga attctttgat
     3601 ttacggttaa gttattatgg tttacggaag gaatggcttg taggaatgct gggagcagaa
     3661 tctacaaagc ttaataatca agcccgtttc attttagaga agatacaggg gaaaattact
     3721 atagagaata ggtcaaagaa agatttgatt caaatgttag tccagagagg ttatgaatct
     3781 gacccagtga aagcgtggaa agaagcacaa gaaaaggctg cagaagagga agattcacaa
     3841 aaccaacatg atgacagttc ctctgattca ggaactcctt caggccctga ttttaattat
     3901 attttaaata tgtctctatg gtctcttact aaagaaaaag ttgaagaact cattaaacag
     3961 agagatacga aagggcgaga ggttaatgat ctcaagagga agtctccttc agatctttgg
     4021 aaagaagact tagcagcatt tgttgaagaa ttggataaag tggaagcaca agagcgggaa
     4081 gatattctgg caggaatgtc tgggaaagca ataaaaggta aagtcggcaa acccaaagta
     4141 aagaaacttc agctggagga gacaatgccc tcgccttacg ggagaagaat tgttccagag
     4201 attactgcta tgaaggcaga tgccagcaga aaattgctga agaagaaaaa gggtgatcct
     4261 gataccacag tagtgaaagt agaatttgat gaagaattta gtggaacacc agcagaaggt
     4321 acaggagagg agacattgac cccatctgct cctgtaaata aaggccccaa acccaaaaga
     4381 gagaagaagg agcctggtac cagggttaga aaaacaccta catcaactgg taaaactaat
     4441 gcgaagaaag tgaagaaacg gaatccttgg tcagatgatg agtccaaatc agagagtgac
     4501 ttggaagaag cagaacctgt ggttatccca agagattctt tgcttaggag agcagctgct
     4561 gaaagaccta agtacacttt tgatttctca gaagaggagg atgatgatgc tgctgctgct
     4621 gatgacagta atgatttaga agaactgaaa gttaaagcat ctcccataac aaatgatggg
     4681 gaagatgaat ttgttccttc agatggctta gataaagatg agtatgcttt ttcttcaggc
     4741 aaatccaaag ctactccaga aaaatcttca aatgacaaaa aaagtcagga ttttggaaat
     4801 cttttttcat ttccttcata ttctcagaag tcagaagatg attcagctaa atttgacagt
     4861 aatgaagaag ataccgcttc tgtttttgct ccatcatttg gtctgaagca aacagataaa
     4921 cttccaagta aaacagttgc tgctaaaaag ggtaaaccac cttcagatac agcccctaaa
     4981 gctaagagag cccctaaaca gaagaagata gtagagacta taaactctga ctcagactct
     5041 gaatttggca ttccaaagaa gactacaaca ccaaaaggta aaggccgagg ggcaaagaaa
     5101 aggaaagcat caggctcaga aaatgaaggt gattataacc ctggaaggaa accatctaaa
     5161 acagccagca agaaaccgaa gaaaacatct tttgatcagg attcagatgt agacatcttc
     5221 ccctcagact tcacttctga accaccggct ctcccacgga ctggtcgggc taggaaagaa
     5281 gtaaaatatt ttgcagagtc tgatgaagaa gaagatgttg attttgcaat gtttaattaa
     5341 gtgcccaaag agcacgaaca tttttcaaaa ataccttgtg ttgtcctttg tcttttctgt
     5401 ctcagaattt tgtacatctg gcttatttta atgtgatgat gtaattgatg gttttttatt
     5461 attgtggtag gccttttaac attttgttct tacacataca gttttatgct cttttttact
     5521 cattgaaatg tcatgtgttg tctgattggc ttgtagaatt gttatagatt accgtgcatt
     5581 agcacagatt ttaattgtca tgattgcaga ctacagatct gctttttgaa atgaaattta
     5641 aacattaaaa atggaactgt g
//
