LOCUS       NM_011623               5217 bp    mRNA    linear   ROD 11-DEC-2024
DEFINITION  Mus musculus topoisomerase (DNA) II alpha (Top2a), mRNA.
ACCESSION   NM_011623
VERSION     NM_011623.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Zhang,K., Zheng,X., Sun,Y., Feng,X., Wu,X., Liu,W., Gao,C., Yan,Y.,
            Tian,W. and Wang,Y.
  TITLE     TOP2A modulates signaling via the AKT/mTOR pathway to promote
            ovarian cancer cell proliferation
  JOURNAL   Cancer Biol Ther 25 (1), 2325126 (2024)
   PUBMED   38445610
  REMARK    GeneRIF: TOP2A modulates signaling via the AKT/mTOR pathway to
            promote ovarian cancer cell proliferation.
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5217)
  AUTHORS   Yao,X., Wang,C., Yu,W., Sun,L., Lv,Z., Xie,X., Tian,S., Yan,L.,
            Li,L. and Liu,J.
  TITLE     BCAS2 regulates oocyte meiotic prophase I by participating in mRNA
            alternative splicing
  JOURNAL   FASEB J 38 (1), e23361 (2024)
   PUBMED   38085152
REFERENCE   4  (bases 1 to 5217)
  AUTHORS   Zhang,W., Zhang,R., Wu,L., Zhu,C., Zhang,C., Xu,C., Zhao,S.,
            Liu,X., Guo,T., Lu,Y., Gao,Z., Yu,X., Li,L., Chen,Z.J., Qin,Y. and
            Jiao,X.
  TITLE     NLRP14 deficiency causes female infertility with oocyte maturation
            defects and early embryonic arrest by impairing cytoplasmic UHRF1
            abundance
  JOURNAL   Cell Rep 42 (12), 113531 (2023)
   PUBMED   38060382
REFERENCE   5  (bases 1 to 5217)
  AUTHORS   Sun,B., Reynolds,K., Saha,S.K., Zhang,S., McMahon,M. and Zhou,C.J.
  TITLE     Ezh2-dependent methylation in oral epithelia promotes secondary
            palatogenesis
  JOURNAL   Birth Defects Res 115 (19), 1851-1865 (2023)
   PUBMED   37435868
REFERENCE   6  (bases 1 to 5217)
  AUTHORS   Baloch,Z., Cohen,S., Fresa,K. and Coffman,F.D.
  TITLE     Modulation of topoisomerase activities by tumor necrosis factor
  JOURNAL   Cell Immunol 160 (1), 98-103 (1995)
   PUBMED   7842491
REFERENCE   7  (bases 1 to 5217)
  AUTHORS   Adachi,N., Miyaike,M., Ikeda,H. and Kikuchi,A.
  TITLE     Characterization of cDNA encoding the mouse DNA topoisomerase II
            that can complement the budding yeast top2 mutation
  JOURNAL   Nucleic Acids Res 20 (20), 5297-5303 (1992)
   PUBMED   1331984
REFERENCE   8  (bases 1 to 5217)
  AUTHORS   Capranico,G., Tinelli,S., Austin,C.A., Fisher,M.L. and Zunino,F.
  TITLE     Different patterns of gene expression of topoisomerase II isoforms
            in differentiated tissues during murine development
  JOURNAL   Biochim Biophys Acta 1132 (1), 43-48 (1992)
   PUBMED   1380833
REFERENCE   9  (bases 1 to 5217)
  AUTHORS   Ura,K. and Hirose,S.
  TITLE     Possible role of DNA topoisomerase II on transcription of the
            homeobox gene Hox-2.1 in F9 embryonal carcinoma cells
  JOURNAL   Nucleic Acids Res 19 (22), 6087-6092 (1991)
   PUBMED   1683482
REFERENCE   10 (bases 1 to 5217)
  AUTHORS   Deffie,A.M., Bosman,D.J. and Goldenberg,G.J.
  TITLE     Evidence for a mutant allele of the gene for DNA topoisomerase II
            in adriamycin-resistant P388 murine leukemia cells
  JOURNAL   Cancer Res 49 (24 Pt 1), 6879-6882 (1989)
   PUBMED   2555055
  REMARK    Erratum:[Cancer Res 1990 Jan 15;50(2):449]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591067.35.
            
            On Jul 26, 2007 this sequence version replaced NM_011623.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D12513.1, SRR9219380.46551.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-144               AL591067.35        167470-167613       c
            145-297             AL591067.35        166264-166416       c
            298-388             AL591067.35        165764-165854       c
            389-452             AL591067.35        165135-165198       c
            453-598             AL591067.35        162220-162365       c
            599-696             AL591067.35        159854-159951       c
            697-909             AL591067.35        159452-159664       c
            910-1083            AL591067.35        158310-158483       c
            1084-1185           AL591067.35        158112-158213       c
            1186-1323           AL591067.35        157839-157976       c
            1324-1462           AL591067.35        155445-155583       c
            1463-1620           AL591067.35        154330-154487       c
            1621-1746           AL591067.35        153898-154023       c
            1747-1857           AL591067.35        153452-153562       c
            1858-1963           AL591067.35        153217-153322       c
            1964-2073           AL591067.35        153014-153123       c
            2074-2166           AL591067.35        152654-152746       c
            2167-2281           AL591067.35        150605-150719       c
            2282-2403           AL591067.35        150384-150505       c
            2404-2552           AL591067.35        149427-149575       c
            2553-2784           AL591067.35        147536-147767       c
            2785-2919           AL591067.35        147250-147384       c
            2920-3120           AL591067.35        146931-147131       c
            3121-3316           AL591067.35        146298-146493       c
            3317-3408           AL591067.35        144774-144865       c
            3409-3567           AL591067.35        144398-144556       c
            3568-3651           AL591067.35        144192-144275       c
            3652-3837           AL591067.35        142586-142771       c
            3838-3928           AL591067.35        141253-141343       c
            3929-4075           AL591067.35        140153-140299       c
            4076-4199           AL591067.35        139274-139397       c
            4200-4243           AL591067.35        139105-139148       c
            4244-4378           AL591067.35        138668-138802       c
            4379-4578           AL591067.35        137631-137830       c
            4579-4750           AL591067.35        137188-137359       c
            4751-5217           AL591067.35        136371-136837       c
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 62.91 cM"
     gene            1..5217
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /note="topoisomerase (DNA) II alpha"
                     /db_xref="GeneID:21973"
                     /db_xref="MGI:MGI:98790"
     exon            1..144
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..4710
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /EC_number="5.6.2.2"
                     /note="DNA topoisomerase II, alpha isozyme; DNA
                     Topoisomerase II alpha"
                     /codon_start=1
                     /product="DNA topoisomerase 2-alpha"
                     /protein_id="NP_035753.2"
                     /db_xref="CCDS:CCDS25370.1"
                     /db_xref="GeneID:21973"
                     /db_xref="MGI:MGI:98790"
                     /translation="MELSPLQPVNENMLMNKKKNEDGKKRLSIERIYQKKTQLEHILL
                     RPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMS
                     CIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGG
                     RNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITF
                     QPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKD
                     KVDETGNSLKVIHEQVNPRWEVCLTMSERGFQQISFVNSIATSKGGRHVDYVADQIVS
                     KLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQ
                     LSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRN
                     STECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINN
                     IIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRH
                     RFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAK
                     EYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPED
                     YLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKRE
                     VKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKD
                     SASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIG
                     TGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAI
                     LDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMT
                     EEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYG
                     LRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKA
                     WKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRN
                     EKEQELNTLKQKSPSDLWKEDLAVFIEELEVVEAKEKQDEQVGLPGKAGKAKGKKAQM
                     CADVLPSPRGKRVIPQVTVEMKAEAEKKIRKKIKSENVEGTPAEDGAEPGSLRQRIEK
                     KQKKEPGAKKQTTLPFKPVKKGRKKNPWSDSESDVSSNESNVDVPPRQKEQRSAAAKA
                     KFTVDLDSDEDFSGLDEKDEDEDFLPLDATPPKAKIPPKNTKKALKTQGSSMSVVDLE
                     SDVKDSVPASPGVPAADFPAETEQSKPSKKTVGVKKTATKSQSSVSTAGTKKRAAPKG
                     TKSDSALSARVSEKPAPAKAKNSRKRKPSSSDSSDSDFERAISKGATSKKAKGEEQDF
                     PVDLEDTIAPRAKSDRARKPIKYLEESDDDDDLF"
     exon            145..297
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            298..388
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            389..452
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            453..598
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            599..696
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            697..909
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1083
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1185
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1186..1323
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1462
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1620
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1746
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..1857
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1858..1963
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2073
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2166
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2281
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2282..2403
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2404..2552
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2553..2784
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2785..2919
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2920..3120
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3121..3316
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3317..3408
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3409..3567
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3568..3651
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3652..3837
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3838..3928
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3929..4075
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4076..4199
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4200..4243
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4244..4378
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4379..4578
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4579..4750
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4751..5217
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5195..5200
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /note="hexamer: AATAAA"
     polyA_site      5217
                     /gene="Top2a"
                     /gene_synonym="Top-2"
                     /note="major polyA site"
ORIGIN      
        1 tcctaaccga caagcgtctg tgaggggagc gtccaggtcc cgcgtcgcgc gtcgcgggtc
       61 gcctagcgtt tcccgctggt tttgtcgctt tccggattct cgagcctctt caggaccgtc
      121 accatggagt tgtcaccgct gcagcctgta aatgaaaata tgctaatgaa caaaaagaag
      181 aatgaagatg gcaagaaaag attgtctatt gaaagaatct atcagaaaaa aacacaactg
      241 gaacatatac tgctccgccc agatacctac attggctctg tggagttagt gacccagcaa
      301 atgtgggttt acgatgaaga tgttggcatt aactacaggg aagtcacttt tgttcctggt
      361 ttgtataaaa tctttgatga gattctagtt aatgctgctg ataacaaaca aagggaccca
      421 aaaatgtctt gtattagagt cacaattgat ccagaaaata atgtaattag catctggaat
      481 aatgggaaag gaatccctgt tgttgaacac aaagttgaga aaatatatgt cccagctctc
      541 atatttggac aactcctgac ctccagtaac tatgacgacg atgagaagaa agtgacaggt
      601 ggtcgaaatg gctatggagc taaactgtgt aacatattca gcaccaaatt tactgtggaa
      661 acagccagta gagagtacaa gaaaatgttc aaacagacgt ggatggataa catggggaga
      721 gctggtgaca tggaactcaa gccctttagt ggagaagatt atacatgtat caccttccag
      781 cctgacttat ctaagtttaa aatgcaaagc ctggacaaag atattgttgc actgatggtc
      841 agaagagcat atgatatcgc tggctccact aaagatgtca aagtctttct taatggaaat
      901 agtctgccag tgaaaggatt ccgcagttac gtggatttgt atttgaagga taaggtagat
      961 gaaactggga attcactgaa ggtgatacat gaacaggtca atccccggtg ggaagtgtgc
     1021 ttaacaatga gcgagagagg cttccagcag attagcttcg tcaacagcat tgcgacttct
     1081 aagggcggca gacatgtcga ttatgtagct gatcagattg tgagcaaact cgttgatgtg
     1141 gtgaaaaaga agaacaaggg cggggttgca gtgaaggctc atcaggtgaa aaatcacatg
     1201 tggatttttg taaatgcctt aattgaaaac ccaacctttg actctcaaac aaaagaaaac
     1261 atgactttac aagccaagag ctttggatca acatgtcaat taagtgaaaa gttcatcaaa
     1321 gctgcaattg gttgtggtat tgtggaaagc atactaaact gggtgaagtt caaggctcaa
     1381 atccaactga acaagaagtg ttcagctgta aaacatacca aaatcaaggg aatccccaaa
     1441 cttgatgatg ccaacgatgc agggagccga aactctactg aatgcacact tatcctgact
     1501 gagggagact cagcaaaaac tctagccgtt tcaggccttg gagtggttgg aagagacaaa
     1561 tatggggtgt tccctcttag aggaaagata ctcaatgtgc gagaagcctc tcataaacag
     1621 atcatggaaa atgctgaaat taacaatatc atcaagattg tgggtctcca gtacaagaaa
     1681 aactatgaag atgaagattc attgaaaact cttcgttatg ggaagatcat gattatgaca
     1741 gatcaggacc aagatggttc ccacatcaaa ggcttgctga tcaattttat ccatcacaat
     1801 tggccatctc ttctgcgaca tcgttttcta gaagaattta tcactcccat tgtaaaggtg
     1861 tccaaaaata agcaagaaat agcattctat agtcttcccg agtttgaaga atggaaaagt
     1921 tcgactccga atcataaaaa gtggaaagtc aaatactaca aaggtttggg caccagcaca
     1981 tcaaaggaag ctaaggaata ttttgcagat atgaaacgac atcgtattca gttcaaatac
     2041 tctggccctg aagatgatgc tgcgatcagc ctggccttta gtaaaaaaca agttgatgat
     2101 cgaaaggaat ggttaactaa ttttatggaa gacagaagac agcggaagtt acttggcctt
     2161 cctgaggatt atttgtatgg acaaagcact tcgtacctga cctataatga cttcataaac
     2221 aaggaactca tcctcttctc caactctgat aatgaaaggt ctatcccatc catggtagac
     2281 ggtttgaaac caggtcagag aaaggttttg tttacttgtt tcaaacggaa tgacaagcga
     2341 gaagtgaagg ttgcccagtt agctgggtca gtggcagaaa tgtcctctta tcaccatggt
     2401 gagatgtcac tgatgatgac cattatcaat ttggcgcaga attttgtggg tagcaataat
     2461 ttgaaccttt tacagcccat tggtcagttt ggaaccaggc tgcatggtgg caaggactca
     2521 gctagtccta ggtacatctt tacaatgctc agccctttgg ctcggttgtt gtttcctcca
     2581 aaagatgatc acacattacg gtttctatat gatgacaacc aacgtgttga gcctgaatgg
     2641 tacattccta taatacccat ggtgctgata aatggtgctg agggaattgg tactgggtgg
     2701 tcctgcaaaa tccccaactt tgatgtgcgt gaagttgtga ataatatcag acggcttctg
     2761 gatggagagg agcccctacc catgctccca agttataaga atttcaaagg tactattgaa
     2821 gaactggctt caaatcagta tgtgattaat ggagaagtag ctattctgga ttctacaacc
     2881 attgaaatct cagagcttcc catccgaacg tggactcaga catataaaga acaggttcta
     2941 gaacccatgt tgaatggaac tgagaagaca ccctctctaa taacagacta tagggaatac
     3001 catacagata ccactgtgaa gtttgtcata aagatgactg aagaaaaact ggcagaggca
     3061 gagagagtcg gcctacacaa agtcttcaaa ctccagagta gtctcacttg caactctatg
     3121 gtgctttttg accatgtagg ttgcttaaag aaatatgaca ctgtgttgga tattctaaga
     3181 gacttctttg agctcaggct taaatattat ggattaagaa aagaatggct tctaggaatg
     3241 cttggtgcag aatcttctaa actgaataat caagctcgct ttatattaga gaaaatagat
     3301 ggcaaaatag tcattgaaaa taaacctaaa aaagaattaa ttaaagttct gattcagaga
     3361 ggctacgact ctgaccctgt gaaagcctgg aaagaagctc agcagaaggt tccagatgaa
     3421 gaagaaaatg aagaaagtga cactgaaacc agcaccagtg actctgcagc cgaggctggg
     3481 ccgaccttca actaccttct cgacatgccc ctgtggtatc tgaccaagga gaagaaggat
     3541 gagctgtgca aacaaagaaa cgagaaggaa caagagctca acacattaaa gcaaaagagt
     3601 ccatcagatc tgtggaagga agatctggct gtttttattg aagaactgga ggttgtcgag
     3661 gccaaggaaa agcaggatga acaagtgggc cttcctggaa aggcggggaa agcaaagggg
     3721 aagaaagcac agatgtgtgc tgacgtcttg ccttctcctc ggggcaaaag agtcatcccc
     3781 caagtgactg tggagatgaa agctgaggca gagaagaaaa ttaggaagaa aattaagagt
     3841 gaaaacgttg agggtacccc tgctgaggat ggtgcggaac caggaagcct ccggcaacga
     3901 atagagaaga agcagaagaa agagccaggt gcaaagaagc agactacatt gccgtttaag
     3961 cctgtcaaaa aagggaggaa gaaaaacccc tggtctgact ctgagtcaga cgtgagcagt
     4021 aatgaaagta acgttgatgt ccctcctcga caaaaagagc aaagaagtgc tgcagcaaaa
     4081 gccaaattca cagtggattt agactcggat gaagatttct caggtttgga tgagaaggat
     4141 gaggatgaag atttcctccc attagatgct actccaccta aggccaaaat ccccccaaaa
     4201 aatactaaaa aagcactgaa gacccaggga agctccatgt cggtagttga tcttgaaagt
     4261 gatgttaagg acagtgtgcc agcttctcca ggcgttcctg ctgctgactt cccagcggaa
     4321 actgaacagt caaagccatc caaaaagacc gtgggagtga agaagacagc aaccaaaagc
     4381 cagtcttcag tctccactgc tggtaccaaa aagagagctg cgccaaaggg aaccaaatca
     4441 gattcagcct tgagtgctcg tgtctcggaa aaacctgctc ctgccaaagc caagaacagt
     4501 cgcaaaagga agccatcttc ttctgatagc tctgactctg attttgagag agcaatttct
     4561 aaaggtgcca cgagcaagaa agcaaaggga gaggaacagg acttccctgt ggacttggaa
     4621 gacacgatag ctcctcgagc caaatctgac cgggcgagga agccaattaa gtacctggag
     4681 gagtccgatg acgatgacga cctcttctga ggcagaagag attgttttag ggatggtttt
     4741 acggagccag ttttataggt agaattcggt catatagaac tggttagttc tagtacagat
     4801 acagtgctca acctctgacg tgatgcattt tgtttaagcc atgagaagtt gctcgtacct
     4861 tttgaacatc ggaggctgga gaaagtcacc ttgactgtgt ctttatgact cagcacaagc
     4921 agcaaggtga ggaaagttag gtcagttacg tagagtactg actctactcg ggatgccttc
     4981 ccaacacgag atccctggga ggaggcagga ctgctcacgg ttacatctcc aggatcaacc
     5041 aggaagtggg atccattgca gatctccata attccaactt gtgactgcca agaccttctg
     5101 tgctgttgtc tcttatttaa gtgctgttat caatgtcttt tgtaaatatt taatatgtct
     5161 gtctgttccg ctaattccaa ctattttgta ctttaataaa tcttctaaac aatgcaa
//
