LOCUS       NM_009408               3763 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus topoisomerase (DNA) I (Top1), transcript variant 1,
            mRNA.
ACCESSION   NM_009408 NM_175299
VERSION     NM_009408.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Lau,M.S., Hu,Z., Zhao,X., Tan,Y.S., Liu,J., Huang,H., Yeo,C.J.,
            Leong,H.F., Grinchuk,O.V., Chan,J.K., Yan,J. and Tee,W.W.
  TITLE     Transcriptional repression by a secondary DNA binding surface of
            DNA topoisomerase I safeguards against hypertranscription
  JOURNAL   Nat Commun 14 (1), 6464 (2023)
   PUBMED   37833256
  REMARK    GeneRIF: Transcriptional repression by a secondary DNA binding
            surface of DNA topoisomerase I safeguards against
            hypertranscription.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Amin,W., Nishio,S., Honjo,T. and Kobayashi,M.
  TITLE     Necessity of HuR/ELAVL1 for the activation-induced cytidine
            deaminase-dependent decrease in topoisomerase 1 in antibody
            diversification
  JOURNAL   Int Immunol 35 (8), 361-375 (2023)
   PUBMED   37086201
  REMARK    GeneRIF: Necessity of HuR/ELAVL1 for the activation-induced
            cytidine deaminase-dependent decrease in topoisomerase 1 in
            antibody diversification.
REFERENCE   3  (bases 1 to 3763)
  AUTHORS   Kobayashi,M., Wakaguri,H., Shimizu,M., Higasa,K., Matsuda,F. and
            Honjo,T.
  TITLE     Ago2 and a miRNA reduce Topoisomerase 1 for enhancing DNA cleavage
            in antibody diversification by activation-induced cytidine
            deaminase
  JOURNAL   Proc Natl Acad Sci U S A 120 (18), e2216918120 (2023)
   PUBMED   37094168
  REMARK    GeneRIF: Ago2 and a miRNA reduce Topoisomerase 1 for enhancing DNA
            cleavage in antibody diversification by activation-induced cytidine
            deaminase.
REFERENCE   4  (bases 1 to 3763)
  AUTHORS   Reijns,M.A.M., Parry,D.A., Williams,T.C., Nadeu,F., Hindshaw,R.L.,
            Rios Szwed,D.O., Nicholson,M.D., Carroll,P., Boyle,S., Royo,R.,
            Cornish,A.J., Xiang,H., Ridout,K., Schuh,A., Aden,K., Palles,C.,
            Campo,E., Stankovic,T., Taylor,M.S. and Jackson,A.P.
  CONSRTM   Genomics England Research Consortium; Colorectal Cancer Domain UK
            100,000 Genomes Project
  TITLE     Signatures of TOP1 transcription-associated mutagenesis in cancer
            and germline
  JOURNAL   Nature 602 (7898), 623-631 (2022)
   PUBMED   35140396
  REMARK    Erratum:[Nature. 2022 May;605(7910):E7. doi:
            10.1038/s41586-022-04812-z.. PMID: 35504971]
REFERENCE   5  (bases 1 to 3763)
  AUTHORS   Krupa,O., Fragola,G., Hadden-Ford,E., Mory,J.T., Liu,T.,
            Humphrey,Z., Rees,B.W., Krishnamurthy,A., Snider,W.D., Zylka,M.J.,
            Wu,G., Xing,L. and Stein,J.L.
  TITLE     NuMorph: Tools for cortical cellular phenotyping in tissue-cleared
            whole-brain images
  JOURNAL   Cell Rep 37 (2), 109802 (2021)
   PUBMED   34644582
REFERENCE   6  (bases 1 to 3763)
  AUTHORS   Baloch,Z., Cohen,S., Fresa,K. and Coffman,F.D.
  TITLE     Modulation of topoisomerase activities by tumor necrosis factor
  JOURNAL   Cell Immunol 160 (1), 98-103 (1995)
   PUBMED   7842491
REFERENCE   7  (bases 1 to 3763)
  AUTHORS   Giros,B., Pohl,M., Rochelle,J.M. and Seldin,M.F.
  TITLE     Chromosomal localization of opioid peptide and receptor genes in
            the mouse
  JOURNAL   Life Sci 56 (18), PL369-PL375 (1995)
   PUBMED   7752808
REFERENCE   8  (bases 1 to 3763)
  AUTHORS   Masaki,S. and Watanabe,T.
  TITLE     The gene Bfsp1 for the lens fiber cell beaded-filament structural
            protein CP94 maps to mouse chromosome 2
  JOURNAL   Genomics 22 (2), 449-450 (1994)
   PUBMED   7806233
REFERENCE   9  (bases 1 to 3763)
  AUTHORS   Kingsmore,S.F., Lo,C.K., Hwang,J., Hui,C.F. and Seldin,M.F.
  TITLE     Genetic mapping of DNA topoisomerase I-related sequences to mouse
            chromosomes 2 and 14
  JOURNAL   Mamm Genome 5 (4), 247-248 (1994)
   PUBMED   8012118
REFERENCE   10 (bases 1 to 3763)
  AUTHORS   Porteus,M.H., Brice,A.E., Bulfone,A., Usdin,T.B., Ciaranello,R.D.
            and Rubenstein,J.L.
  TITLE     Isolation and characterization of a library of cDNA clones that are
            preferentially expressed in the embryonic telencephalon
  JOURNAL   Brain Res Mol Brain Res 12 (1-3), 7-22 (1992)
   PUBMED   1372074
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591673.11 and AL590414.4.
            
            On Oct 27, 2022 this sequence version replaced NM_009408.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D10061.1, L20632.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-282               AL591673.11        10502-10783
            283-307             AL591673.11        11129-11153
            308-410             AL590414.4         19040-19142
            411-534             AL590414.4         31591-31714
            535-590             AL590414.4         33388-33443
            591-686             AL590414.4         36588-36683
            687-762             AL590414.4         39217-39292
            763-869             AL590414.4         42636-42742
            870-985             AL590414.4         47064-47179
            986-1107            AL590414.4         51642-51763
            1108-1230           AL590414.4         52512-52634
            1231-1418           AL590414.4         53723-53910
            1419-1563           AL590414.4         54117-54261
            1564-1707           AL590414.4         61585-61728
            1708-1893           AL590414.4         63021-63206
            1894-1962           AL590414.4         63986-64054
            1963-2077           AL590414.4         64653-64767
            2078-2205           AL590414.4         66327-66454
            2206-2300           AL590414.4         69519-69613
            2301-2450           AL590414.4         69867-70016
            2451-3763           AL590414.4         70392-71704
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 80.96 cM"
     gene            1..3763
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /note="topoisomerase (DNA) I"
                     /db_xref="GeneID:21969"
                     /db_xref="MGI:MGI:98788"
     exon            1..282
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             250..2553
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /EC_number="5.6.2.1"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     topoisomerase 1; DNA topoisomerase I"
                     /codon_start=1
                     /product="DNA topoisomerase 1 isoform 1"
                     /protein_id="NP_033434.2"
                     /db_xref="CCDS:CCDS16995.1"
                     /db_xref="GeneID:21969"
                     /db_xref="MGI:MGI:98788"
                     /translation="MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKDKDK
                     DREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKERRKEEKIRAAGDA
                     KIKKEKENGFSSPPRIKDEPEDDGYFAPPKEDIKPLKRLRDEDDADYKPKKIKTEDIK
                     KEKKRKSEEEEDGKLKKPKNKDKDKKVAEPDNKKKKPKKEEEQKWKWWEEERYPEGIK
                     WKFLEHKGPVFAPPYEPLPESVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIF
                     RKNFFKDWRKEMTNDEKNTITNLSKCDFTQMSQYFKAQSEARKQMSKEEKLKIKEENE
                     KLLKEYGFCVMDNHRERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDA
                     KVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETAR
                     RLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCS
                     LRVEHINLHPELDGQEYVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDL
                     FDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENVPAKILSYNRA
                     NRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKDQLADARRDLKSAKADAKVMKDAKT
                     KKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGV
                     PIEKIYNKTQREKFAWAIDMTDEDYEF"
     exon            283..307
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            308..410
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            411..534
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            535..590
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            591..686
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            687..762
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            763..869
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            870..985
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1107
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1230
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1418
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1563
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1707
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1708..1893
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..1962
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1963..2077
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2078..2205
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2206..2300
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2301..2450
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2451..3763
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3738..3743
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /note="hexamer: AATAAA"
     polyA_site      3763
                     /gene="Top1"
                     /gene_synonym="D130064I21Rik; Top-1"
                     /note="major polyA site"
ORIGIN      
        1 gaaatgcgaa cttaggctgt tacacaactg ctggggtctg ttctcgctgt ccgcccgccc
       61 ggcagtctgt cagtcgcgtc gctaccgccg ctgcctcctc cactgtcccg ggagtctcgg
      121 gccggtggct gctgtcgccg tctgttcgtg cttctcagga gcttcgtccg cccgttcagg
      181 ccgtccctgc cgtccgcctc tcttcccccg cgctgcctct gagcccgcag cgttcgcacg
      241 ccggccgaca tgagcgggga ccatctccac aacgattccc agatcgaagc ggatttccga
      301 ttgaatgatt ctcacaaaca caaagataaa cacaaagatc gagaacaccg gcataaggag
      361 cacaagaagg ataaggataa ggaccgggaa aagtctaagc atagcaacag tgaacataaa
      421 gattctgaaa agaaacacaa agagaaagag aaaaccaaac acaaagatgg cagctcagaa
      481 aaacataaag acaaacataa agacagagac aaggaaagac gaaaggagga aaagattaga
      541 gctgctgggg atgcaaaaat aaagaaggaa aaggaaaatg gcttctctag tccgccacga
      601 attaaagatg aacctgagga tgatggctat tttgctcctc caaaagagga tattaagcca
      661 ttgaagaggc ttcgagatga agatgatgct gattataaac ctaagaaaat taagacagaa
      721 gatatcaaga aggagaagaa aagaaaatca gaagaggaag aggatggtaa acttaaaaag
      781 cccaagaata aagataaaga taaaaaagtt gctgagccag ataataagaa aaagaagccg
      841 aaaaaggaag aggaacagaa gtggaaatgg tgggaagaag aacgttatcc agaaggcatc
      901 aaatggaaat tcctagagca taaagggcct gtcttcgctc caccatatga gcctctgcca
      961 gagagtgtca agttttacta tgatggtaaa gttatgaagc tgagtcctaa agcagaagaa
     1021 gtagctacat tctttgcaaa aatgcttgac cacgaatata ctactaagga aatattcagg
     1081 aaaaatttct ttaaagattg gagaaaggaa atgactaatg acgaaaaaaa tacgattacc
     1141 aacctaagca aatgcgactt tacacagatg agccagtatt tcaaagcgca gtcagaagct
     1201 cggaaacaga tgagcaagga agaaaaattg aaaatcaaag aagaaaatga aaagttattg
     1261 aaagaatacg gcttttgtgt aatggataat catagagagc ggattgccaa cttcaagata
     1321 gagcctccgg ggcttttccg aggccgaggg aaccacccca agatgggtat gctgaagaga
     1381 aggatcatgc ctgaggacat catcatcaac tgtagcaaag acgcaaaggt tccttctccc
     1441 cctcctggac ataaatggaa ggaagtccga catgataaca aggttacttg gctggtctcc
     1501 tggacagaga atatccaagg ttctatcaaa tatatcatgc tgaatcccag ttcacgaatc
     1561 aagggtgaga aagactggca gaagtatgag actgcgcggc gtctgaagaa gtgtgtggac
     1621 aagatccgaa atcagtatcg ggaagactgg aagtccaaag agatgaaagt tcgtcagaga
     1681 gctgtagcac tgtacttcat tgataagctt gctctgagag caggcaatga gaaggaagaa
     1741 ggagagacgg cagacactgt gggctgctgt tcacttagag tggaacacat caatttacac
     1801 ccagaattgg atggtcagga gtatgtggtg gaatttgact ttcctgggaa ggactcaatc
     1861 agatactata acaaagtccc agttgagaaa cgagttttta aaaacttaca actgtttatg
     1921 gagaacaagc agcctgagga cgatcttttt gatcgactta atactggtat tctaaataaa
     1981 catcttcagg atctcatgga gggcttgact gccaaggtgt tccgtaccta caatgcctcc
     2041 atcacactac agcagcagct taaagagctc acagcccctg atgagaatgt accagcgaag
     2101 attctatctt ataaccgtgc caatcgagct gttgcaattc tttgtaacca ccagagggcg
     2161 ccaccaaaga cctttgagaa gtcaatgatg aacttgcagt ctaagattga tgccaagaaa
     2221 gatcagttag cagatgctcg aagggacctg aaaagtgcta aggctgatgc caaagtcatg
     2281 aaggatgcaa agaccaagaa ggtagtagag tcaaaaaaga aggctgtaca gagactagaa
     2341 gagcagttga tgaagctgga agttcaagcc acagatcgag aggagaacaa acaaattgcc
     2401 ttggggacct ccaaactcaa ttatctggac cctaggatca cagtggcttg gtgcaaaaag
     2461 tggggggtcc caattgagaa gatttacaac aaaacccaga gggagaagtt tgcttgggcc
     2521 attgatatga ctgatgagga ctacgagttt tgaccagtct cggcaggggt tctgtgaaaa
     2581 ggaacagtgt ggtttgggga agatggataa actgagcctc acttgccctc acacccgagg
     2641 gagaggccac aagtcttaac aaaccaacat ctttgcaaaa agatagacct ggagatacta
     2701 taagggagag ctgagccagt tgtcctatgg acaacttatt taaaaatatt tcagatatca
     2761 aaattctagc tgtatgattt gttttgaatt ttgtttttac tttcaagaga gcaagtggat
     2821 gggaatttgt cagcatcctg ccaggcaaat tcagttttgc tgcaatgttt ggattctctc
     2881 agctactgta tgtgaagtct gattatattg gtgcgttttt acagttaggg ttttgcaata
     2941 acttctatat tttaatagaa acaaattcct aaactccctc ctctccccta tttcaggaat
     3001 ttaaaattaa gtagaacaaa acaaaaaccc agcgcaccta ttacagctgt cattctatcg
     3061 tcatgggaat cagttttcat taaacttgaa gcagttgtga cattggcagt gttttggttc
     3121 agacacctat tcacagaaaa gcatgatggg aaaatatttc ctgacttgag tgttcctttt
     3181 taaatgtgaa tttttatttc gttttaatta ttttaaaata tttaagcctt cttcttgatc
     3241 ttaaagatcg tgtagcttgg ggttggggag ggatgaaggg cgagtgagtc taaggataat
     3301 gtaataatca gtgactgaaa ccattttccc atcatccttt gttctgacca ttctttgtac
     3361 cctttaagag atccatcttt ttctttggaa accccagtct ttcatttgaa agatgttatt
     3421 gtataaaaac ttccacaggt caataaattt agaggaaaat gagcatttgg tccaagaaaa
     3481 acaaaaacca aaacaaaaca aaaaaggaaa aataatcaag attttagggc ttttattttt
     3541 tcttttgtaa ttgtgtaaaa aatggaaaaa aaaacataaa aaggaaaatt ttaatgtgaa
     3601 gacttttttt ttgctataat cattagtttt agaggcattg ttagttagtg tgtgtgcaga
     3661 gtccattccc acatctttcc tcaagtatct tctattttta tcatgaattc ccttttaatc
     3721 aactgtaggt tatttaaaat aaactcctac aacttaacgg aaa
//
