LOCUS       NM_001289895           10003 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus tensin 1 (Tns1), transcript variant 2, mRNA.
ACCESSION   NM_001289895
VERSION     NM_001289895.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10003)
  AUTHORS   Shih,Y.P., Sun,P., Wang,A. and Lo,S.H.
  TITLE     Tensin1 positively regulates RhoA activity through its interaction
            with DLC1
  JOURNAL   Biochim Biophys Acta 1853 (12), 3258-3265 (2015)
   PUBMED   26427649
  REMARK    GeneRIF: studies demonstrate that TNS1 binds to DLC1 and fine-tunes
            its RhoGAP activity toward RhoA and that the TNS1-DLC1-RhoA
            signaling axis is critical in regulating cellular functions that
            lead to angiogenesis
            Erratum:[Biochim Biophys Acta Mol Cell Res. 2018 Sep;1865(9):1383.
            doi: 10.1016/j.bbamcr.2018.05.016.. PMID: 29866430]
REFERENCE   2  (bases 1 to 10003)
  AUTHORS   Dina,C., Bouatia-Naji,N., Tucker,N., Delling,F.N., Toomer,K.,
            Durst,R., Perrocheau,M., Fernandez-Friera,L., Solis,J., Le
            Tourneau,T., Chen,M.H., Probst,V., Bosse,Y., Pibarot,P.,
            Zelenika,D., Lathrop,M., Hercberg,S., Roussel,R., Benjamin,E.J.,
            Bonnet,F., Lo,S.H., Dolmatova,E., Simonet,F., Lecointe,S.,
            Kyndt,F., Redon,R., Le Marec,H., Froguel,P., Ellinor,P.T.,
            Vasan,R.S., Bruneval,P., Markwald,R.R., Norris,R.A., Milan,D.J.,
            Slaugenhaupt,S.A., Levine,R.A., Schott,J.J., Hagege,A.A. and
            Jeunemaitre,X.
  CONSRTM   PROMESA investigators; MVP-France; Leducq Transatlantic MITRAL
            Network
  TITLE     Genetic association analyses highlight biological pathways
            underlying mitral valve prolapse
  JOURNAL   Nat Genet 47 (10), 1206-1211 (2015)
   PUBMED   26301497
REFERENCE   3  (bases 1 to 10003)
  AUTHORS   Enciso,J.M., Konecny,C.M., Karpen,H.E. and Hirschi,K.K.
  TITLE     Endothelial cell migration during murine yolk sac vascular
            remodeling occurs by means of a Rac1 and FAK activation pathway in
            vivo
  JOURNAL   Dev Dyn 239 (10), 2570-2583 (2010)
   PUBMED   20737513
REFERENCE   4  (bases 1 to 10003)
  AUTHORS   Chambers,D., Wilson,L.J., Alfonsi,F., Hunter,E., Saxena,U.,
            Blanc,E. and Lumsden,A.
  TITLE     Rhombomere-specific analysis reveals the repertoire of genetic cues
            expressed across the developing hindbrain
  JOURNAL   Neural Dev 4, 6 (2009)
   PUBMED   19208226
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10003)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10003)
  AUTHORS   Ishii,A. and Lo,S.H.
  TITLE     A role of tensin in skeletal-muscle regeneration
  JOURNAL   Biochem J 356 (Pt 3), 737-745 (2001)
   PUBMED   11389681
REFERENCE   7  (bases 1 to 10003)
  AUTHORS   Chen,H., Ishii,A., Wong,W.K., Chen,L.B. and Lo,S.H.
  TITLE     Molecular characterization of human tensin
  JOURNAL   Biochem J 351 Pt 2 (Pt 2), 403-411 (2000)
   PUBMED   11023826
REFERENCE   8  (bases 1 to 10003)
  AUTHORS   Leiter,E.H., Tsumura,H., Serreze,D.V., Chapman,H.D., Rabin,D.U.,
            Lan,M.S. and Notkins,A.L.
  TITLE     Mapping to chromosomes 1 and 12 of mouse homologs of human protein
            tyrosine phosphatase, receptor-type, related genes encoding
            pancreatic beta cell autoantigens
  JOURNAL   Mamm Genome 8 (12), 949-950 (1997)
   PUBMED   9383296
REFERENCE   9  (bases 1 to 10003)
  AUTHORS   Lo,S.H., Yu,Q.C., Degenstein,L., Chen,L.B. and Fuchs,E.
  TITLE     Progressive kidney degeneration in mice lacking tensin
  JOURNAL   J Cell Biol 136 (6), 1349-1361 (1997)
   PUBMED   9087448
REFERENCE   10 (bases 1 to 10003)
  AUTHORS   Jankowski,S.A. and Gumucio,D.L.
  TITLE     Genes for tensin, villin and desmin are linked on mouse chromosome
            1
  JOURNAL   Mamm Genome 6 (10), 744-745 (1995)
   PUBMED   8563175
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC117757.23 and AC116419.32.
            
            On Jun 18, 2019 this sequence version replaced NM_001289895.1.
            
            Transcript Variant: This variant (2) lacks two in-frame exons in
            the 3' coding region, compared to variant 1, resulting in an
            isoform (b) that is shorter than isoform a.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-257               AC117757.23        130379-130635       c
            258-372             AC117757.23        85286-85400         c
            373-410             AC117757.23        74696-74733         c
            411-452             AC116419.32        15589-15630
            453-494             AC116419.32        28217-28258
            495-545             AC116419.32        29438-29488
            546-601             AC116419.32        34671-34726
            602-782             AC116419.32        36394-36574
            783-833             AC116419.32        38562-38612
            834-956             AC116419.32        39553-39675
            957-1021            AC116419.32        40036-40100
            1022-1105           AC116419.32        41381-41464
            1106-1218           AC116419.32        45400-45512
            1219-1279           AC116419.32        45840-45900
            1280-1355           AC116419.32        45997-46072
            1356-1485           AC116419.32        46627-46756
            1486-1551           AC116419.32        49477-49542
            1552-1668           AC116419.32        50745-50861
            1669-3198           AC116419.32        77825-79354
            3199-3395           AC116419.32        89669-89865
            3396-3471           AC116419.32        90722-90797
            3472-3564           AC116419.32        94458-94550
            3565-3763           AC116419.32        103665-103863
            3764-4827           AC116419.32        106006-107069
            4828-4914           AC116419.32        109922-110008
            4915-5046           AC116419.32        110686-110817
            5047-5139           AC116419.32        111176-111268
            5140-5217           AC116419.32        112044-112121
            5218-5289           AC116419.32        113706-113777
            5290-5458           AC116419.32        113974-114142
            5459-5527           AC116419.32        114841-114909
            5528-5560           AC116419.32        115304-115336
            5561-10003          AC116419.32        117101-121543
FEATURES             Location/Qualifiers
     source          1..10003
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 38.17 cM"
     gene            1..10003
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /note="tensin 1"
                     /db_xref="GeneID:21961"
                     /db_xref="MGI:MGI:104552"
     exon            1..257
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..5705
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /note="isoform b is encoded by transcript variant 2"
                     /codon_start=1
                     /product="tensin-1 isoform b"
                     /protein_id="NP_001276824.1"
                     /db_xref="CCDS:CCDS48288.2"
                     /db_xref="GeneID:21961"
                     /db_xref="MGI:MGI:104552"
                     /translation="MGCTVSLVCCEALEPLPSCGPQPPGTPPGPARPERCEPGGAAPD
                     PRRRLLLQPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQA
                     KVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQ
                     PQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQML
                     KSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADP
                     HNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQR
                     RYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGI
                     YNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVV
                     FGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWD
                     SYDNFSGHREDGMEEVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNH
                     VEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREK
                     QGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQ
                     SGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIP
                     VHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEP
                     QLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPV
                     AALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQP
                     LLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSP
                     ASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHR
                     VAGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTI
                     ALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPA
                     DILLHPTGEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSF
                     VSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAAL
                     SYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGT
                     NTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPS
                     LGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRH
                     LGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYP
                     GLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANG
                     MASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLK
                     DQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGV
                     KLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLK
                     QGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQ
                     RKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAE
                     LDPNQPASAIVNFVSKVMLSAGQKR"
     exon            258..372
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            373..410
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            411..452
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            453..494
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            495..545
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            546..601
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            602..782
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            783..833
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            834..956
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1021
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1105
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1218
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1279
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1355
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1485
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1551
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1668
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..3198
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            3199..3395
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            3396..3471
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            3472..3564
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            3565..3763
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            3764..4827
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            4828..4914
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            4915..5046
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            5047..5139
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            5140..5217
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            5218..5289
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            5290..5458
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            5459..5527
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            5528..5560
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     exon            5561..10003
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9977..9982
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /note="hexamer: TATAAA"
     polyA_site      10003
                     /gene="Tns1"
                     /gene_synonym="1110018I21Rik; 1200014E20Rik;
                     E030018G17Rik; E030037J05Rik; Tns"
                     /note="major polyA site"
ORIGIN      
        1 cgtcgcagac gcctggggcc gagaggtcgc tggggctggg gggcgggccg gctcgcgggt
       61 agtccgcgtc cccgccccgg gcccgccggg tcccgggccg gatgggctgc accgtgagcc
      121 tggtgtgctg cgaggcgctc gagcctctgc cctcctgcgg cccgcagccc cccgggactc
      181 cgccgggccc cgcgcggccc gagcgctgtg agcccggggg tgcggcgccc gacccgagga
      241 ggcggctgct gcttcagcca gaagacctgg aggcccccaa gacccaccac ttcaaggtga
      301 aggccttcaa gaaggtgaag ccctgtggca tctgccggca ggccatcact cgggaaggct
      361 gtgtttgcaa agtctgcagc ttctcctgtc atcggaaatg ccaggccaag gtggctgccc
      421 cttgcgttcc tccgtccagc catgaactgg tgcccatcac cacagagacc gtacccaaga
      481 atgtagtaga tgtgggggaa ggagactgcc gggttggaag ttctccgaaa aacctggagg
      541 agggcggctc catgagagtt tctccaagca tacagccaca accacaatca cagcctacca
      601 gtctctctag aaacacgagt gtgagccgag ccatggaaga cagttgtgag ctggacctgg
      661 tgtatgtcac cgaacgcatc atcgccgtgt ccttccctag cacagccaat gaggagaact
      721 tccgcagcaa cctccgtgaa gtggctcaga tgttgaagtc caagcatggg ggcaactacc
      781 tgctattcaa cctctctgaa cagaggcctg acatcaccaa gctgcatgct aaggtcctgg
      841 aattcggctg gcctgacctg cacacaccgg ccctggagaa gatatgcagt gtctgtaagg
      901 ccatggacac ctggctcaat gcagaccccc acaatgtcgt tgttctccac aacaagggaa
      961 accgaggcag gattggggtt gtcattgcag cgtatctgca ctacagcaac atttcagcca
     1021 gtgctgacca ggctctggac cggtttgcaa tgaagagatt ctatgaggac aagatcgtgc
     1081 ccattggcca gccatcccag aggagatatg tgcactactt cagtggccta ctctctggct
     1141 ccatcaaaat gaacaacaag cctttgtttc tgcatcatgt gatcatgcac ggcatcccca
     1201 actttgagtc taaaggaggg tgccggccct ttctccgcat ctaccaagcc atgcaacctg
     1261 tgtacacatc tggcatttac aacatccctg gagacagcca ggccagcatc tgtatcacca
     1321 ttgaaccagg gctgctcttg aagggagaca tcttgctgaa gtgttatcat aagaaattcc
     1381 ggagcccggc tcgagacgtc atcttccgag tgcagttcca cacctgtgcc atccatgact
     1441 tgggggttgt ttttgggaag gaagatctgg atgaggcctt caaagatgat cgatttcctg
     1501 actatggcaa agtggagttt gtcttttcct atggaccaga gaaaattcaa ggcatggaac
     1561 acctggagaa tgggcccagc gtatctgtag actataacac ttctgacccc ctcatccgct
     1621 gggactccta tgacaacttc agtgggcatc gtgaggatgg catggaagaa gtggtgggac
     1681 acacccaagg gccgctggat gggagcctgt acgccaaggt gaagaagaag gattccctga
     1741 atggcagttc ggggcctgtc actactgcac ggcctgccct gtcagccact cccaaccacg
     1801 tggaacacac cctgtctgtg agcagcgact ctggcaactc cacagcctcc actaagacag
     1861 acaagaccga tgagccagtc tctggggcta ccactgcccc tgctgccctg agtcctcagg
     1921 agaagaaaga gctggaccgc ctactaagtg gctttggtgt ggatagagaa aagcaaggcg
     1981 ccatgtaccg ggctcagcaa ctcagatccc atccaggcgg gggcccgact gtgccctccc
     2041 ctggccgcca cattgtccca gcccaggttc acgtcaatgg tggggccctg gcatctgagc
     2101 gggagacaga cattctagat gatgagctgc ccatccagga cgggcagagt gggggcagca
     2161 tgggaacgct gtcctccctg gatggggtca ccaacaccag tgaaagtggc tacccagaga
     2221 ccctgtcccc actgaccaat ggcctggaca aaccctattc cacagaacct gtgctcaacg
     2281 gcggaggcta cccctatgag gctgccaacc gggtgattcc tgtccacagc agtcactcgg
     2341 cccccattcg accctcctac tctgctcagg agggtttagc tggctaccag cgtgagggac
     2401 cccacccagc ctggtcacag caagtgacca gtgcccactg tggctgtgac cccagtggtc
     2461 tattcagatc ccagtccttt ccagatgtgg agccccagct gccccaagct ccaacccgtg
     2521 ggggtagtag ccgagaggct gtgcagaggg gtctgaattc atggcaacag cagcagcccc
     2581 acccacctcc ccgccagcag gagagatccc cactgcagag tcttgcccgc agcaagccta
     2641 gcccccagct gtcagcagag acccccgtag ctgctctccc agagttccca agggcagcgt
     2701 cccagcagga gatcgagcag tccatcgaaa ccctcaatat gttgatgctg gacctggagc
     2761 cagcctccgc agctgcccct cttcataagt cccagagtgt ccctggggcc tggccagggg
     2821 cttctccact ctcttcccag cccctcttgg ggtcttcccg ccagtcccac ccacttaccc
     2881 agtcccgatc tggctatatc cccagtgggc attctctggg aaccccggag ctggtctctt
     2941 ctggcaggcc ttattcacct tacgattatc agctgcatcc agctggatcc aaccagagtt
     3001 tccatccaaa aagtccagcc tcttccacct tccttccaag ccctcatagc tctgcggggc
     3061 ctcaggagcc cccagcctct ctccctggtc tcatcgctca gccccagctc ccaccaaagg
     3121 aaactacatc agatccctcc cgaactccag aggaagagcc gctgaacctg gaagggctgg
     3181 tagcccatcg ggtagcaggg gttcaggctc gggagaggca gcctgcagag cccccaggcc
     3241 cactccggag gcgggcagcc agtgatggac agtatgaaaa ccagtctccg gaggccacat
     3301 ccccccgtag tcctggggtc cgctcccccg tccagtgcgt ctcccctgag ctggctctta
     3361 ccattgccct caatcctgga gggcgcccca aagagcctca tttgcatagc tacaaggagg
     3421 cctttgagga gatggagggg acctccccaa gcagcccgcc acacagtgtg gcgcgctctc
     3481 ctccaggtct ggccaagaca cccctgtctg ctctgggcct gaaaccccac aacccagcag
     3541 acatcttgct gcaccccaca ggtgagcccc ggagctatgt ggagtccgtg gcgaggacag
     3601 cggtggcagg gcctcgagct caggacgttg agcctaagag cttcagcgca ccagctgctc
     3661 acgcctatgg gcacgagaca cccctgagga acgggacccc agggggctcc tttgtctccc
     3721 cgagccccct ctccacaagc agccccatcc tcagtgctga cagcacttcc gtgggcagtt
     3781 tcccatcagt ggtgagcagt gaccagggtc cccggacacc cttccagcct atgctggact
     3841 ccagcatccg ctcaggcagc ttggggcagc cgagccctgc agcactgagt tatcagagct
     3901 cctcgcctgt ccctgttggt ggcagcagct acaacagccc tgactactcc ctccaaccat
     3961 tcagctcttc tccagaaagc caaggccagc cacagtacag tgcggccagt gtccacatgg
     4021 tacctgggag ccctcaggca cgacacagga cagtgggtac caacactcca cctagccctg
     4081 gttttggccg gcgggccgtc aaccccacca tggctgcccc tggtagtccc agtttgagcc
     4141 atcggcaggt gatgggtcca tctggtccag gtttccatgg taacgtggtt tctggccacc
     4201 cagccagtgc agctaccact cctggaagcc ccagcctggg ccggcaccca gtgggaagcc
     4261 atcaagttcc aggcctccac agcagtgtgg tcaccactcc gggaagccca agcctgggcc
     4321 gacaccctgg tgcccaccaa gggaatctgg cctccagtct ccacagcaat gcagtcatca
     4381 gccctgggag cccaagcttg ggccggcacc tgggtgggtc tggatccgtg gttcctggaa
     4441 gccccagctt ggaccggcat gcagcttatg gtggctactc tacccctgag gaccgaagac
     4501 caacactgtc ccggcaaagc agtgcctctg gctaccaggc cccatccaca ccgtcattcc
     4561 ctgtctctcc tgcctactac cctggcctga gcagccctgc cacctcaccc tcgccagact
     4621 cagcagcctt ccggcagggg agccccacac cagccttacc agagaagcga agaatgtcag
     4681 tgggagaccg ggcaggcagc ctccccaact atgccaccat caatgggaaa gtgtcttcct
     4741 cgcctgtcgc caatggcatg gccagtggga gcagcaccgt ctccttctct cacacgcttc
     4801 cggacttttc taagtattcg atgccagaca atagtccgga gactcgggct aaagtgaagt
     4861 ttgtccagga cacttccaag tattggtaca agcctgagat ctccagagaa caggccatcg
     4921 cactcctaaa ggaccaggag ccaggagcct tcatcatccg ggacagccac tccttccgag
     4981 gggcttatgg gctggccatg aaggtgtcct caccccctcc aaccatcact cagcagggca
     5041 agaaaggaga catgacccat gagctggtga ggcactttct gatcgagaca ggccctagag
     5101 gagtgaagct caagggttgt cccaatgagc caaactttgg ttccctttct gccctggtct
     5161 atcagcactc cgtcatccca ctggccctgc cctgtaaact ggtcattcca agccgagacc
     5221 ccacagatga atcaaaagat agctcaggcc ctgccaactc aaccactgac ctgctgaagc
     5281 aaggagcagc ttgcaatgtg ctcttcgtca actctgtgga tatggagtca ctcaccgggc
     5341 cacaggccat ttccaaagcc acatctgaga ctctggctgc cgaccccaca ccggctgcca
     5401 ccattgttca cttcaaggtc tctgcccaag gaatcacact gactgacaat caaagaaagc
     5461 tcttctttag aagacactac cccctcaaca ctgtcacctt ctgtgacctg gatccacaag
     5521 aaagaaagtg gatgaagaca gagggaggcg cccctgctaa gctctttggc ttcgtggccc
     5581 ggaagcaggg cagcaccaca gacaacgctt gccacctctt cgcagagctt gaccccaacc
     5641 agcctgcgtc tgccatcgtc aacttcgtct ccaaggtcat gctgagtgct ggccagaaga
     5701 gatgaacgaa atcgggagcc ctcgcccaag gctagggcat cggggatggg gtgtgtgggg
     5761 aggggacctg tgaatcctga ccaccttgaa tccagaacca ggactttggg tcgcccgtgg
     5821 agaaggaaag aagaaagtgc aatgttggga gaggaaagtg aatggcggag gggggaggca
     5881 agcaagcggg aggaagagga aggaaggaag aagagaggca ctcagaatcc cctgtatgga
     5941 tggaagccac acccccaaag cctctccagc tagctggggc tcctccccct tctgagagac
     6001 ttgcgctccc gcagggaaca cacaggcctc cttgggggcg ggggaccata tttcacagag
     6061 caatggaaaa gctccctctc cccaaccctc ctgctctgag agaagaaatc gaactgaggc
     6121 ttcttctctg gaggtaaggt tgctggtggc cagcgtatga catcaagcag tgacagtttt
     6181 tcacattttg ctcacccggg gcacccgtgg tctgtgcttg ctctgactcc ttgttgcagg
     6241 gcataaatgt gcccccccct ccacgcgctg atcgatactc tgcctgtgtc cactcaactc
     6301 agattacaaa cagctctctc tgcttgtctg tgtcccttgt gatacactta aacacactgt
     6361 ccaaagaagg ctagggaggg ttggttacag ggtggcaggg tcccaggtcg gatgacaagc
     6421 tgtctcctgc ggaagtgtag cttgtgggta tagagttctg tctgcacctg ccctctagtg
     6481 accctgggcc tgtcctctgt agagactctg gtacagaggg catgggcacc tgcagcatta
     6541 tagctgttct gtctcctgga gaccaaagct ggcttggagg aaaaaatgat gcccacagtc
     6601 ttgggaaaaa tacctgctgg gaaggatggc atcttttctg cgcggtgggg ctcccggaga
     6661 actgggcatg cgcattgtgc cgtggggctt aggatgagtt ggacacgcgc actgacaatg
     6721 cctaggactg ccttactccc ttccctcagg ccagttcctg ggccagcact agccttgaat
     6781 ttgctcatcc gcttgaaacc tactggggga tggggcccgt gcctttctga cctaccctgt
     6841 cctatatggt cctcgtgttg gcttatccca ggagcctact tcccagagtc ctgcctaggg
     6901 cacccgggga agcccgtccc cacttcccca ctctgtaagt gttttggagt ctgactattg
     6961 cagacaccct ctggctgggc tgctcagggt gacatcatag cacctctggg aatgctgccg
     7021 aagaacccca tggtcctccc acaataccaa agcttccctg tttccccttc tgcctccgtt
     7081 tccccagcag gaccacactg cctgtgggac aaaggacagg atgtatgaat ttaggccaaa
     7141 ggtcctatgg tccatttgca gggttggaaa ggcccttgcc atctcttagg cctccattta
     7201 agcccatggg ggcccctagg ggtggcattg tactgataaa taaatatata taatatatac
     7261 atgagacata ctgatagaat ctgtaagcta ataaaaaata taagaaaaag gttaaaaaaa
     7321 aaagaatagg taaattgaca agaaatattt attgttttcc cttattgcct tattgtccct
     7381 ggccacaaac tgttcatctc ttctctaatg tgtaagtgga gcctgaactg caggggctct
     7441 ttatattcca aacaacccag ggttagcctt ggcctcgatc ctggccttgg cctccctagg
     7501 ctatatctgg gactgggggg atgacatgct cccactctgg ctccttgttc tctggcaaac
     7561 cgtagcgtgg ctgccagcat cctgtagcgc ggatagctgc ttccatggcc acaggcctga
     7621 accggtgtgt atttggggat ctggatgtgt atggccacgg tgtggcagct tttggatttt
     7681 tttcctccgg ggagcattga ctgtgtggac attgtttgag gagctagaaa gaagatcggc
     7741 tggggatcac gagcccctac ttcccaaaga ataaatgggc ttagtgatca aggttcttca
     7801 aggggtcttt ctccctctga gatattcaag gcaaatgtct ggatgtcgca tttcctcacc
     7861 ccccacctca tgaatcctac ttctccccaa catagaagta gacatttctc atcccattct
     7921 ttgtaaatgt ccacacccta ccgcaaagtc tggggagtct tctggctgga atgctgttca
     7981 cgtctgcatc cagccctggg atcggccttc gtgtatggtt tcaccttccc atgctgcact
     8041 ctttcctgag ccatgtggga gtcgtgtgaa tgaccaaggc agatgctagc aagtggtgct
     8101 tccttctcag cacgtctcta ggaagcatgg acacccgagt gtggcttttc tcaaagaaag
     8161 gtgaagaagg ggcctcagtt cttacagtgg ggaccacact gctgcctgcc cccttcccaa
     8221 ctgtgattct cacacactca catgcgtgca cacacacaca cacacacaga ccacacgtgc
     8281 acacagcact ccatatgcaa ctctgtattc ttgccaaaga tttcctttaa acaagagcac
     8341 ttttacccaa tggctctttt gtgaatgatc tgtttcgtta ttgtttattg ttgagtctgt
     8401 atatcaacag ggagagtgtt gtctgctctt aaacaggaat gaaagggggg aggggagaca
     8461 ggagagaaca cggagaccag tgatgtcctt aacaagaccc ctcagctaca gcccaggtcc
     8521 aaactgcctc tggccactca gaagatgagg ttggcttggc acttcactgt ccctgatatc
     8581 tggaggtgag atggcagaga ggggcaccag tggagccagg attggggcct agggaagccc
     8641 ccaatggttc tggaggcagg gataagtgtg aagggctggg ctaggacagg atggacagga
     8701 aggctgggag aactaacagt tggatactga agttgctact gcggacctgt ggctcccttg
     8761 gccagccatg ttttgggaaa gagttcacat cttaggtttg aaagggagca tttgagtctt
     8821 ctcactgacc tcttcctagc agcttagata gcctcagaca tgactgccag gattcctgat
     8881 atgccctggc tttggagagt ccctagggat gcccactcgg gggtgtaggg aagacactgg
     8941 ctgtgcgtcc ttctaggaag gggctttcag gagagagagt tcttgtgctg tctgagaagt
     9001 gcaggggaga caaatgaatg cagtgtccat ccagctctgg gttttcttta tcctgcctcc
     9061 cgagacttct gacccccctt tcccagcttc agatcttggg gagatccatc caaggggttc
     9121 agccctctcc tgcctccatg ccttgggtcc ctgaagagct tagaacccaa agctagggcc
     9181 acccctgcac ttaggccttg gctgaagatc ttgagactgg tgtttccgtg gtcattggtg
     9241 acaggatgtg tgcgctcgag tgtgtaagtg tgggtgtgtg ggtgtgtgtg tgtggtctgc
     9301 tcatctcttt agggaacttt gaggtgggcg gtggcatatg gaggggtgtc aggttctatg
     9361 tgacccagta atgagaagag cttgaaggtg aggcctgagg ggccacagat gagtcatgtt
     9421 ttagtgctga tgacagaggg gccggtcgca tgggatgagg ctcggtgggc ctcttgcttg
     9481 ttgtctcctg tctcctcaaa tggctgaaaa cttggagggt ctcggttgtt ttccatgcct
     9541 ggcccctcac agcagcgagg ccactgaaga gcaacacacc cagtgtgtgt cagtgttctc
     9601 agcccagccc tccctgagaa cccacatttg gctcgtatgt atgtgagcac gttggggtgc
     9661 gcataagcta tcaccatctt cttctcttct atcatccaaa tcaaatgtaa gaagtccctc
     9721 acatccgatt agcccaactc tgtctcccag gccccctgct gggaaaccca tacccagcat
     9781 ccctcccctg ccctggccct cggctgtgta tttatagatg gaaatatact ttatattttg
     9841 tatcattgtg cctatagcct ctgccacctt gtataaaccc tgatgtatgc taagtgttct
     9901 ggatatgaat gtatgtacac tgatgctgcc cccctctgta cagcggcttg gcctctcacg
     9961 atccattgta tggctttata aatgataaag ttacacaaac cca
//
