LOCUS       NM_144925               8493 bp    mRNA    linear   ROD 26-JUN-2024
DEFINITION  Mus musculus trinucleotide repeat containing 6a (Tnrc6a), mRNA.
ACCESSION   NM_144925 XM_486002 XM_901687 XM_915472 XM_982893 XM_992542
VERSION     NM_144925.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Liu,H., Li,H., Jiang,Z., Jin,S., Song,R., Yang,Y., Li,J., Huang,J.,
            Zhang,X., Dong,X., Mori,M., Fritzler,M.J., He,L., Cardoso,W.V. and
            Lu,J.
  TITLE     A local translation program regulates centriole amplification in
            the airway epithelium
  JOURNAL   Sci Rep 13 (1), 7090 (2023)
   PUBMED   37127654
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  AUTHORS   Wei,W., Chen,Q., Liu,M., Sheng,Y., OuYang,Q., Feng,W., Yang,X.,
            Ding,L., Su,S., Zhang,J., Fang,L., Vidal-Puig,A., Wang,H.Y. and
            Chen,S.
  TITLE     TRIM24 is an insulin-responsive regulator of P-bodies
  JOURNAL   Nat Commun 13 (1), 3972 (2022)
   PUBMED   35803934
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8493)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8493)
  AUTHORS   Hildebrandt,M.R., Wang,Y., Li,L., Yasmin,L., Glubrecht,D.D. and
            Godbout,R.
  TITLE     Cytoplasmic aggregation of DDX1 in developing embryos: Early
            embryonic lethality associated with Ddx1 knockout
  JOURNAL   Dev Biol 455 (2), 420-433 (2019)
   PUBMED   31330130
REFERENCE   5  (bases 1 to 8493)
  AUTHORS   Guo,H., Kazadaeva,Y., Ortega,F.E., Manjunath,N. and Desai,T.J.
  TITLE     Trinucleotide repeat containing 6c (TNRC6c) is essential for
            microvascular maturation during distal airspace sacculation in the
            developing lung
  JOURNAL   Dev Biol 430 (1), 214-223 (2017)
   PUBMED   28811219
REFERENCE   6  (bases 1 to 8493)
  AUTHORS   McKee,A.E., Minet,E., Stern,C., Riahi,S., Stiles,C.D. and
            Silver,P.A.
  TITLE     A genome-wide in situ hybridization map of RNA-binding proteins
            reveals anatomically restricted expression in the developing mouse
            brain
  JOURNAL   BMC Dev Biol 5, 14 (2005)
   PUBMED   16033648
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 8493)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   8  (bases 1 to 8493)
  AUTHORS   Yang,Z., Jakymiw,A., Wood,M.R., Eystathioy,T., Rubin,R.L.,
            Fritzler,M.J. and Chan,E.K.
  TITLE     GW182 is critical for the stability of GW bodies expressed during
            the cell cycle and cell proliferation
  JOURNAL   J Cell Sci 117 (Pt 23), 5567-5578 (2004)
   PUBMED   15494374
REFERENCE   9  (bases 1 to 8493)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 8493)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY720212.1, AK147327.1, CD802917.1, AC138364.8 and BF020619.1.
            
            On or before Nov 2, 2006 this sequence version replaced
            XM_486002.3, XM_915472.2, XM_901687.2, XM_992542.1, XM_982893.1,
            NM_144925.2.
            
            Summary: This gene encodes a member of the trinucleotide repeat
            containing 6 protein family. The protein is highly similar to a
            human protein that functions in post-transcriptional gene silencing
            through the RNA interference (RNAi) and microRNA pathways. The
            human protein associates with messenger RNAs and argonaute proteins
            in cytoplasmic bodies known as GW-bodies or P-bodies, and
            inhibiting its expression delocalizes other GW-body proteins and
            impairs RNAi and microRNA-induced gene silencing. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147327.1, SRR1660813.251091.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-296               BY720212.1         1-296
            297-2202            AK147327.1         2-1907
            2203-2784           CD802917.1         18-599
            2785-5581           AK147327.1         2490-5286
            5582-5582           AC138364.8         137287-137287       c
            5583-7161           AK147327.1         5288-6866
            7162-8031           AC138364.8         133970-134839       c
            8032-8493           BF020619.1         1-462               c
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 67.42 cM"
     gene            1..8493
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /note="trinucleotide repeat containing 6a"
                     /db_xref="GeneID:233833"
                     /db_xref="MGI:MGI:2385292"
     exon            1..448
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     CDS             444..6134
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /note="GW182 autoantigen"
                     /codon_start=1
                     /product="trinucleotide repeat-containing gene 6A protein"
                     /protein_id="NP_659174.3"
                     /db_xref="CCDS:CCDS57583.1"
                     /db_xref="GeneID:233833"
                     /db_xref="MGI:MGI:2385292"
                     /translation="MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAA
                     QKKATEQKIKVPEQIKPSVSQPQPANSDNGTSTATSTNNNAKRATASNQQPPPPQQQQ
                     PQQEQQQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSE
                     SSAVTNQQPQNNGEVQNSKSQSDINHNTSGSHYENCQRGPVSSTSDCSTSCKNAVNDL
                     LEKEAWPSAPGSDPELAPECIDADSASNSESERNITVMASGNTGGEKDGLRNSTGLGS
                     QSKFVVGSSSNNVGHGSSTGPWGFPHGALISTCQVSVDAPESKPESSNNRMNAWGTVS
                     SSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQNI
                     NSKVSGSSTHGTWGSLQETCEPEVSGTQKVSFSGQPQNITTETTGPNNTTNFMTSSLP
                     NSGSVQNNELPTSNPGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGESKTGGSYGTTWG
                     AYGSNYSGDKCAGPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWEPGTVNS
                     QSSPWGSGNGANSGGSRRGWGSPAQNTGTGLSSVEWNKLPSNQHSNDSANGNGKKLTN
                     GWKSTEEDDQGSATSQTNEQNSVWAKAGGTVESDGSAESTGRLEEKVTGESQSRDRRK
                     IDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGS
                     SATQTFNSGACTDKTSPNSNDTSSVSGWGDPKPTLRWGDSKGSNCQGGWEDDSAATGM
                     IKSNQWGGCKEDKSTWNDSQKSKQGWGDGQKSSQGWSISAGDNWGESSRSNHWGEANK
                     KSSSGGSDSDRSISGWNELGKTSSFTWGNNINPNNSSGWDESSKPNSSQGWGDPPKCN
                     QSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATSKPPGTGWLGGPIPAPAKEEEPTGW
                     EEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNIPEASGRSDQQAQMHRLLPA
                     ASAVSSKETSSGSGWGEPWAEPSTPATTVDNGTSAWGKPIDSGPSWGEPITAASNAST
                     WGSSSVGPQSLSKSGPKSMQDGWCGDDMPLPGSRPTGWEEEEDVEIGMWNSNSSQELN
                     SSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEDAWINPFVKQFSNISFSRDSPE
                     ENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYF
                     DKNGNPNMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAP
                     NNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSAPSANRQQQDQ
                     QGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQPLHQPAMKSFLDNVMPHTTPELQKG
                     PSPVNAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSMSVNTSLDQNSSKHGA
                     ISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEP
                     WKGYPNIDPETDPYVTPGSVINSLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSS
                     IRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRP
                     PPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQ
                     IDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAE
                     FASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDVNHWNGAGLSG
                     ANCGDLHGTSLWGTPHYSTSLWGPPSSDPRGISSPSPINAFLSVDHLGGGGESM"
     exon            449..496
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            497..584
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            585..606
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            607..990
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            991..3573
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            3574..3750
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            3751..3926
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            3927..3959
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            3960..4040
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            4041..4092
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            4093..4235
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            4236..4373
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            4374..4502
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            4503..4580
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            4581..4788
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            4789..4920
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            4921..5079
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            5080..5220
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            5221..5328
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            5329..5550
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            5551..5621
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            5622..5761
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     exon            5762..8479
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7721..7726
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /note="hexamer: AATAAA"
     polyA_site      7754
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /note="major polyA site"
     regulatory      8455..8460
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /note="hexamer: AATAAA"
     polyA_site      8479
                     /gene="Tnrc6a"
                     /gene_synonym="2010321I05Rik; 3110054G10Rik; CAGH26;
                     D130023A07Rik; GW182; Tnrc6"
                     /note="major polyA site"
ORIGIN      
        1 gaaacttgtg tcgcagtaaa gaaggaggag gggatggtcc gaggcaagcc cgagccagcc
       61 tcggcgaaaa aggccgcgct ccgctcttgc caggccccag agccgctcgt ggggaacctc
      121 ggagttcccc gacagctccg gcgaagtcct ggacgcgtca gacagttgtc aagtggctgc
      181 ctcgtccggc aggcctagtt gctaggacct ccatcttggg gctcccgggc cacggcgccc
      241 ccggaagggg ttgcgagtgg ggcattcact tccggtctgg ggcctgcggc ggcagcggtg
      301 gcggcggcgg cggcggcggc agcgggacgg tgtagaaaat ggcgctggtg cagcggctcg
      361 ggcctgtccc cgcggcgctg cggagggctt gaggctcgcg agcctcgttc cccgcgcctc
      421 acgcgctagt gcactttaca cacatgagag aattggaagc taaagctacc aaagacgtag
      481 agagaaatct tagcagggat ttagtgcaag aagaagaaca gttgatggaa gaaaagaaaa
      541 agaaaaaaga cgacaagaaa aagaaggaag ctgctcaaaa gaaggccact gaacaaaaaa
      601 tcaaagtgcc agaacagata aagcccagtg taagccagcc tcagcctgcc aactctgata
      661 acggcacttc cacagcaacc agcactaata ataatgccaa gcgagctaca gccagcaatc
      721 agcagccgcc gccaccgcag cagcagcagc cgcagcaaga gcagcagcag cagcagccac
      781 aagccttgcc tcggtatcca cgtgaagtac ctccacgatt tcgccaccag gaacacaaac
      841 agcttctgaa gaggggtcag cattttcctg tcatagcagc aaacttggga tctgctgtta
      901 aggtgttaaa cagccaatca gaaagcagtg ctgtaacaaa tcaacagcca caaaataacg
      961 gagaggtgca gaacagcaag agccagtcag atataaatca taatacttca ggatcccatt
     1021 atgaaaattg ccagcgggga cctgtgtctt ctacaagtga ctgtagcaca agctgtaaga
     1081 atgctgtaaa cgacttgttg gaaaaagaag catggccctc agcccctggc agtgatcctg
     1141 agttggctcc agaatgtata gatgctgatt ctgcctccaa ttctgagtca gagagaaaca
     1201 tcactgtcat ggcttcaggg aacacaggtg gtgagaaaga tggccttcgg aacagcactg
     1261 gacttggttc tcaaagcaaa tttgtggttg gtagcagcag caataatgtg ggccatggaa
     1321 gtagtactgg cccatggggc tttccccatg gagccctaat aagcacatgt caggtctctg
     1381 tggatgctcc tgaaagcaaa ccagaaagta gtaacaatag gatgaatgct tggggcactg
     1441 taagttcttc atcaaatgga gggttaaatc caagcacttt gaattcagct agcaaccatg
     1501 gtgcctggcc agtattagaa aacaatggac ttgccctaaa agggcctgta gggagtggga
     1561 gttctggcat caatattcag tgtagtacca taggccagat gcctaacaat cagaatatca
     1621 actctaaagt cagtggctct tctacccatg gtacctgggg tagccttcag gaaacttgtg
     1681 agcctgaagt aagtggtaca cagaaggttt cattcagtgg tcaacctcag aatatcacca
     1741 ctgaaacgac tggaccaaat aacactacta actttatgac ctctagttta ccaaactccg
     1801 gttcagtaca aaataatgaa ctgcctacta gtaatccagg ggcctggcgt gtgagcacaa
     1861 tgaatcatcc tcagatacag gctccgtcag ttatgaatgg cacttccctt tctcacctta
     1921 gtaatggaga gtcaaaaact ggaggctcct acggtactac atggggtgcc tatggttcta
     1981 attactctgg cgacaaatgt gcaggcccta atggccaagc caatggtgac actgtgaatg
     2041 caactctaat gcagcctggc ataaatgggc ctatgggcac taactttcaa gttaatacaa
     2101 ataaaggggg aggtgtatgg gagcctggga cagtgaattc ccagagttca ccatggggaa
     2161 gtggaaatgg tgcaaattct ggaggaagtc gaagaggatg gggaagtcct gcacagaaca
     2221 ctggcactgg tctatccagt gtcgagtgga acaaactgcc tagcaaccag cattccaatg
     2281 acagtgcaaa tggcaatggt aagaagctta caaatggatg gaaatctact gaggaagacg
     2341 atcagggttc tgccacatct cagacaaatg agcaaaacag tgtgtgggcc aaagcaggag
     2401 gcacagtgga gagtgatggt agtgcagaga gcactggacg ccttgaagaa aaagtaaccg
     2461 gggaaagtca gagtagagat agaagaaaaa ttgatcagca cacattactc caaagcattg
     2521 taaacagaac tgacttagat ccacgtgtcc tatccaactc tgggtgggga cagactccta
     2581 ttaagcagaa tactgcctgg gatacagaga catcaccaag aggggaaaga aagactgaca
     2641 atgggacaga ggcctgggga agctctgcaa cacagacttt taactcaggg gcatgtacag
     2701 ataagactag ccctaatagt aatgatacct catctgtatc agggtggggt gatcccaaac
     2761 ctactctgag gtggggagat tccaaaggct caaactgcca gggggggtgg gaagatgact
     2821 ctgctgctac aggaatgatc aagagcaacc agtggggggg ttgcaaggaa gacaagtcta
     2881 catggaatga ttcgcaaaag agcaaacaag gctggggtga cggacaaaag tcaagccaag
     2941 gttggtccat ttctgccggt gataactggg gagaatcttc caggagtaac cattggggtg
     3001 aggccaataa gaaatccagc tcaggaggca gtgacagtga caggtccatt tctggttgga
     3061 acgaacttgg gaaaactagt tcttttactt ggggaaataa tataaatcca aataactcat
     3121 cgggatggga tgaatcttct aaaccgaact cttcccaggg gtggggagac cctccaaagt
     3181 gtaatcagtc tctaggttgg ggagattcat caaagccagt tagttctcca gattggaaca
     3241 agcaacaaga cattgttgga tcatggggaa tcccaccagc caccagcaag cctcctggta
     3301 caggctggct cgggggacct attcctgctc cagcaaagga ggaagaaccc acaggctggg
     3361 aggagccatc cccagaatct atacgaagaa aaatggagat cgatgatgga acttcagctt
     3421 ggggagatcc aagcaaatac aactacaaaa atgtgaacat gtggaataaa aacatcccgg
     3481 aagccagcgg ccgctcagac cagcaagcgc agatgcaccg gctattgcca gccgcaagtg
     3541 ccgtctcaag caaggagacc agcagtggct ctggctgggg tgagccttgg gcagagcctt
     3601 ctactccagc cacaactgtg gataatggta cttcagcctg gggcaagccc atagacagtg
     3661 gtcccagctg gggagaaccc attactgcag catccaatgc atccacgtgg ggctccagct
     3721 ctgttggtcc acagtcatta agcaaatctg ggcccaaatc tatgcaagat ggctggtgtg
     3781 gtgatgatat gccattgcct ggaagtcgac ccactggctg ggaagaggag gaggatgtag
     3841 agattggaat gtggaacagt aactcatctc aagagcttaa ctcatcttta aattggccac
     3901 catataccaa gaaaatgtca tcaaagggtc tgagtggcaa aaaaaggaga agggaaaggg
     3961 gaatgatgaa aggcggaaac aaacaagagg acgcatggat aaatccattc gttaaacagt
     4021 tttcaaatat cagtttttcg agagactcac cagaagaaaa tgtacagagc aataagatgg
     4081 atctttctgg aggaatgtta caagacaagc ggatggagat tgataaacat agcctgaata
     4141 ttggtgatta caatcgaacg gtcgggaaag gccctgggtc tcggcctcag atttccaaag
     4201 agtcttccat ggagcgcaat ccttactttg ataagaatgg caatcccaat atgtttggtg
     4261 ttggaaacac agcagcacaa ccccggggca tgcagcagcc tccagcacaa cctctcagtt
     4321 catctcagcc taatcttcgt gctcaagtgc ctcctccatt actctcccct caggttccag
     4381 tttcattgct gaagtatgca ccaaacaacg gtggcctgaa tccgctcttt ggccctcaac
     4441 aggtagccat gctgaaccag ctatcccaac taaaccagct ttctcagatc tcccagttac
     4501 agcgattgtt agcgcagcag cagagggcac agagtcagag aagcgcgcct tctgctaacc
     4561 gacagcagca ggaccagcag ggtcgacctc ttagtgtgca gcagcagatg atgcaacagt
     4621 ctcgtcaact tgatccaagc ctgctggtga agcagcagac tccgccttct cagcagccgc
     4681 tccatcagcc agccatgaag tccttccttg acaatgtcat gccccacact acacccgagc
     4741 tgcagaaagg gccatcgcca gtaaatgctt tcagcaactt tcctataggc ttgaactcaa
     4801 acttgaatgt aaatatggat atgaacagta ttaaagagcc acagtcacga ctaaggaagt
     4861 ggacaacagt ggacagcatg tctgtgaaca cgtcgttgga tcagaactcc agcaaacatg
     4921 gtgctatttc aagtggtttt aggctggaag agtctccatt tgttccctat gactttatga
     4981 atagcagtac ttcaccagcc agtcctccag gttcaatagg agatggctgg ccacgtgcca
     5041 aatcgcctaa cggctctagc agtgttaact ggccaccaga atttcgccct ggtgaaccat
     5101 ggaaaggtta tccaaacatt gaccctgaaa ctgaccctta cgtcactcct ggcagtgtca
     5161 taaacagtct ttcaattaat actgtgcggg aagttgacca cctcagggac aggaacagtg
     5221 ggtcatcctc atccttgaac accacgctgc cttcaactag tgcctggtca tccattcgtg
     5281 cctccaacta caatgttccc ctcagcagta cagcacaaag cacttcagcc agaaatagtg
     5341 attccaaatt gacgtggtct cctggttcag ttacaaacac ctctctggct catgagctgt
     5401 ggaaagtccc tttgccacct aaaaacatca ctgctccgtc ccgcccacct ccggggctga
     5461 ctggtcagaa gccacccttg tctacgtggg ataattctcc ccttcgtgta ggtggaggat
     5521 ggggaaattc tgatgccaga tataccccag gctccagctg gggtgagagc agctcaggga
     5581 gaataaccaa ttggcttgtt ctgaagaacc tcacacctca gattgatggc tcaactctgc
     5641 gcaccctgtg catgcagcat ggcccactga ttacattcca tctgaacctc ccacatggaa
     5701 atgctctggt ccgttacagt tcaaaagaag aggtagtgaa ggcacaaaag tctctgcaca
     5761 tgtgtgtgct ggggaacact actattctcg ctgagtttgc cagtgaagag gagatcagtc
     5821 gcttctttgc acaaagtcag tctctgaccc cttctcctgg ctggcagtct ctcgggtcca
     5881 gccagagccg gctgggctct ctcgactgtt cccactcgtt ctccagccgg actgatgtca
     5941 atcactggaa tggtgctggg ctgtcgggag ctaactgtgg agaccttcac ggcacttccc
     6001 tctgggggac cccacattat tccacaagcc tgtggggtcc cccgagcagc gacccccggg
     6061 gaattagcag cccatccccc attaatgctt ttctttctgt tgaccacctg ggtgggggtg
     6121 gggagtccat gtaatggcat aggtatagac gtaaactgtg acctcacgaa gagaaggaaa
     6181 ggagcactaa gttgggctcg ccgcctgcag ccagggcacc tgtgggaaca gctgttttct
     6241 gcacattctc cactttgttt tccccaaaac atatcagttt gaatacttga atcatgcagg
     6301 ccaatattat aatgtgaaag ggtatctatc tatttacact cccaaacagc gccatacagc
     6361 taccgtgtgg aatgagctca cttgtgtatt catcatgttt agccttcgga ttccttcttt
     6421 cctccatttc catccccatc cccacctttt atttttattt gtattttttg caaaaccgtt
     6481 ttggggctga cgtatgagct tttacctttg cactgagtga tgttctccgt ctaatcggca
     6541 atatgggggc agctgttcca gtgtagatgt ttactcaagg gtgttctcaa ggcgtgcgct
     6601 ctctactatg cctcatgttg cctaccttag tgtggtatcg tggagttgaa aagatcaagt
     6661 taagatactg acgtaggatt cttaatgaaa gtattgcacc agttttttca tgttgtaaaa
     6721 ctaaagaatt tcgctctaca gtttgagaaa ctgtggccac cgctgtgacc tgcagcccgc
     6781 ctgctgccca ggacgggccc tgcactttga acaggctttc catgttgttt tgaaggttcc
     6841 cacggtgaac cttcttgttt acagattttt ttgtttgttt tttgagaaaa aaaatgttta
     6901 ctcttccatc atttaaaaaa aaaaaattaa aagacaaaaa aaaaaaaaaa ggagggtggt
     6961 ttaaaagacg cttctatctc tgggaaaaga gcagcagttg gccatgttct tctgttttct
     7021 attcctgtcc taaatcaaag agcatggttc tcaggaaacc agttccccgg tcaaaaaatc
     7081 ttgtagtttc ttataggaaa gaagaaaacc caacttttag cactgatact acgtattgct
     7141 ctgttcaaca ttttctctgc caaaaaaaga aaaaaaaaaa aaaaagaaaa gaaaaaagaa
     7201 aaaaacgcgc ttcgagctgg agttgccgtc tgctttcaga tgctgtcctt tagtagtgag
     7261 tgatgatggt ttgctaatcg ctaggtgact tttgtaaccc catcagtggc tctcatgtct
     7321 ctgctctctt gtgactgtgt tcatgtttaa ctgttgtacc ttaaagccga gatcagtaac
     7381 tatgcatact gtaaccaaga cattgggctt acagagttgt ttgttgtata aaaaatttta
     7441 aatgttgttg caaactaacg agttacacca ttttaaaatt tctttcctcc ctagcccctg
     7501 ttgttttgcc cacaaatggt attataatgc ttgcttagtc aaagagagac taaacaaggg
     7561 taaactttta acagtacaga atacgccatc atttcattgc cttgattcta actgtttgtg
     7621 tcctaagatg caaaagaggt cagtggcttt taactgttta caataggatg tgattgtaaa
     7681 tgtacagttt ggttgtgttt gaattatgaa atcttcaaat aataaaccat gactttttgg
     7741 ctgctcaaca ttaattgtct cttttttgtg gatttatttg taggctcttt tataatgaaa
     7801 gttacaaagt tgctgcatgt gagagttctt ctcatagagc ggcagattaa aaatctaagc
     7861 aaatatttga acattttacc tgaacttgtc cacaatcacc ctgagataat gtgagaacag
     7921 tgggaactgt agcttgctcc tcctcccctg agcatctgtg ggagcttgtt gttgcccaaa
     7981 gctcttctct ggcttcacct tccccaccat ctgtgcccat ctcaagcctc agcgggacca
     8041 ttgggacacg aagctgactg ccgtgacagc gtcctagtgt taacctctca tacctcacac
     8101 agtggggatg ccaccacctg gactggcctc tcttccctgt gcccttcgcc cctcctcact
     8161 gaaggagctc tgggcacaga acctgtatga cttcaaatca cgctccactt gccaggtgta
     8221 agctaatgtt ggacaccgta acctaagcaa atgtttaatg cattcggtgt gtgttgatgt
     8281 ccatactggt ttttccaaaa accaaaggta gctttgaaaa accatgtgtg gagatgtttg
     8341 gaccgttaag ctgagtgacc gtttggggct ttgagtagta tatatctgac ttcatgactt
     8401 cgttaattgt attgttaaaa gtgtttggga gtttttttgc gcttgttatg tggaaataaa
     8461 gtgtttgatt taaaaaataa aaaaaaaaaa aaa
//
