LOCUS       NM_178716               8412 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Mus musculus transportin 1 (Tnpo1), transcript variant 1, mRNA.
ACCESSION   NM_178716
VERSION     NM_178716.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Zhou,J., Wang,R., Zhang,J., Zhu,L., Liu,W., Lu,S., Chen,P., Li,H.,
            Yin,B., Yuan,J., Qiang,B., Shu,P. and Peng,X.
  TITLE     Conserved expression of ultra-conserved noncoding RNA in mammalian
            nervous system
  JOURNAL   Biochim Biophys Acta Gene Regul Mech 1860 (12), 1159-1168 (2017)
   PUBMED   29055695
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   Sharma,P., Abbasi,C., Lazic,S., Teng,A.C.T., Wang,D., Dubois,N.,
            Ignatchenko,V., Wong,V., Liu,J., Araki,T., Tiburcy,M., Ackerley,C.,
            Zimmermann,W.H., Hamilton,R., Sun,Y., Liu,P.P., Keller,G.,
            Stagljar,I., Scott,I.C., Kislinger,T. and Gramolini,A.O.
  TITLE     Evolutionarily conserved intercalated disc protein Tmem65 regulates
            cardiac conduction and connexin 43 function
  JOURNAL   Nat Commun 6, 8391 (2015)
   PUBMED   26403541
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8412)
  AUTHORS   Lim,Y.S. and Tang,B.L.
  TITLE     A role for Rab23 in the trafficking of Kif17 to the primary cilium
  JOURNAL   J Cell Sci 128 (16), 2996-3008 (2015)
   PUBMED   26136363
  REMARK    GeneRIF: Co-immunoprecipitation and affinity-binding studies
            revealed that Rab23 exists in a complex with Kif17 and importin b2
            implying that Kif17 needs to bind to regulatory proteins like Rab23
            for its ciliary transport
REFERENCE   4  (bases 1 to 8412)
  AUTHORS   Di Benedetto,A., Sun,L., Zambonin,C.G., Tamma,R., Nico,B.,
            Calvano,C.D., Colaianni,G., Ji,Y., Mori,G., Grano,M., Lu,P.,
            Colucci,S., Yuen,T., New,M.I., Zallone,A. and Zaidi,M.
  TITLE     Osteoblast regulation via ligand-activated nuclear trafficking of
            the oxytocin receptor
  JOURNAL   Proc Natl Acad Sci U S A 111 (46), 16502-16507 (2014)
   PUBMED   25378700
  REMARK    GeneRIF: Association with Tnpo1 is necessary for Oxtr nuclear
            localization. This is required for Oxt-induced osteoblast
            differentiation. osteoblast differentiation.
REFERENCE   5  (bases 1 to 8412)
  AUTHORS   Bjorkblom,B., Maple-Grodem,J., Puno,M.R., Odell,M., Larsen,J.P. and
            Moller,S.G.
  TITLE     Reactive oxygen species-mediated DJ-1 monomerization modulates
            intracellular trafficking involving karyopherin beta2
  JOURNAL   Mol Cell Biol 34 (16), 3024-3040 (2014)
   PUBMED   24912681
  REMARK    GeneRIF: DJ-1 import is mediated by an oxidative stress-dependent
            interaction with karyopherin beta2.
REFERENCE   6  (bases 1 to 8412)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   7  (bases 1 to 8412)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 8412)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 8412)
  AUTHORS   Muhlhausser,P., Muller,E.C., Otto,A. and Kutay,U.
  TITLE     Multiple pathways contribute to nuclear import of core histones
  JOURNAL   EMBO Rep 2 (8), 690-696 (2001)
   PUBMED   11493596
REFERENCE   10 (bases 1 to 8412)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154849.2 and AC129085.6.
            
            On Jan 7, 2022 this sequence version replaced NM_178716.3.
            
            Transcript Variant: This variant (1) encodes the longer protein
            (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK030580.1, SRR10223969.13086.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-93                AC154849.2         109906-109998       c
            94-207              AC154849.2         74332-74445         c
            208-283             AC154849.2         72121-72196         c
            284-433             AC154849.2         68170-68319         c
            434-540             AC154849.2         62275-62381         c
            541-674             AC154849.2         59259-59392         c
            675-756             AC154849.2         53895-53976         c
            757-879             AC154849.2         50701-50823         c
            880-998             AC154849.2         48624-48742         c
            999-1059            AC154849.2         48083-48143         c
            1060-1228           AC154849.2         47363-47531         c
            1229-1381           AC129085.6         205845-205997       c
            1382-1607           AC129085.6         204967-205192       c
            1608-1779           AC129085.6         203590-203761       c
            1780-1866           AC129085.6         202093-202179       c
            1867-2133           AC129085.6         200516-200782       c
            2134-2221           AC129085.6         200347-200434       c
            2222-2320           AC129085.6         198604-198702       c
            2321-2416           AC129085.6         198059-198154       c
            2417-2492           AC129085.6         195312-195387       c
            2493-2592           AC129085.6         194359-194458       c
            2593-2667           AC129085.6         192532-192606       c
            2668-2810           AC129085.6         191625-191767       c
            2811-8412           AC129085.6         184223-189824       c
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 52.24 cM"
     gene            1..8412
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /note="transportin 1"
                     /db_xref="GeneID:238799"
                     /db_xref="MGI:MGI:2681523"
     exon            1..93
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     CDS             79..2775
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /note="isoform 1 is encoded by transcript variant 1;
                     karyopherin (importin) beta 2; importin beta-2;
                     karyopherin beta-2"
                     /codon_start=1
                     /product="transportin-1 isoform 1"
                     /protein_id="NP_848831.2"
                     /db_xref="CCDS:CCDS26717.2"
                     /db_xref="GeneID:238799"
                     /db_xref="MGI:MGI:2681523"
                     /translation="MVWDRQTKMEYEWKPDEQGLQQILQLLKESQSPDTTIQRTVQQK
                     LEQLNQYPDFNNYLIFVLTKLKSEDEPTRSLSGLILKNNVKAHFQNFPNGVTDFIKSE
                     CLNNIGDSSPLIRATVGILITTIASKGELQNWPDLLPKLCSLLDSEDYNTCEGAFGAL
                     QKICEDSAEILDSDVLDRPLNIMIPKFLQFFKHSSPKIRSHAVACVNQFIISRTQALM
                     LHIDSFIENLFALAGDEEAEVRKNVCRALVMLLEVRMDRLLPHMHNIVEYMLQRTQDQ
                     DENVALEACEFWLTLAEQPICKDVLVRHLPKLIPVLVNGMKYSDIDIILLKGDVEEDE
                     TIPDSEQDIRPRFHRSRTVAQQHEEDGIEEEDDDDDEIDDDDTISDWNLRKCSAAALD
                     VLANVYRDELLPHILPLLKELLFHHEWVVKESGILVLGAIAEGCMQGMIPYLPELIPH
                     LIQCLSDKKALVRSITCWTLSRYAHWVVSQPPDTYLKPLMTELLKRILDSNKRVQEAA
                     CSAFATLEEEACTELVPYLAYILDTLVFAFSKYQHKNLLILYDAIGTLADSVGHHLNK
                     PEYIQMLMPPLIQKWNMLKDEDKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVNLV
                     QKTLAQAMLNNAQPEQYEAPDKDFMIVALDLLSGLAEGLGGNIEQLVARSNILTLMYQ
                     CMQDKMPEVRQSSFALLGDLTKACFQHVKPCIADFMPILGTNLNPEFISVCNNATWAI
                     GEISIQMGIEMQPYIPMVLHQLVEIINRPNTPKTLLENTAITIGRLGYVCPQEVAPML
                     QQFIRPWCTSLRNIRDNEEKDSAFRGICTMISVNPSGVIQDFIFFCDAVASWINPKDD
                     LRDMFCKILHGFKNQVGDENWRRFSDQFPLPLKERLAAFYGV"
     exon            94..207
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            208..283
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            284..433
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            434..540
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            541..674
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            675..756
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            757..879
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            880..998
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1059
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1228
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1381
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1607
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1608..1779
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..1866
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1867..2133
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2134..2221
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2222..2320
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2321..2416
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2492
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2493..2592
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2593..2667
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2668..2810
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2811..8412
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8393..8398
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /note="hexamer: AATAAA"
     polyA_site      8412
                     /gene="Tnpo1"
                     /gene_synonym="D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN"
                     /note="major polyA site"
ORIGIN      
        1 gttccgcagc catttcaggc cccggacggg aggcagcgcc gcttcggcag aaaggcccga
       61 gcgccggagg cctctgggat ggtgtgggac cggcaaacca agatggagta tgagtggaaa
      121 cctgacgagc aagggcttca gcagatcctg cagctgctca aggagtccca gtccccagac
      181 accaccatcc agaggaccgt gcaacaaaaa ctggaacaac tcaatcaata tccagatttt
      241 aacaactact tgatttttgt tcttacaaaa ttaaaatctg aagatgagcc cacacggtca
      301 ttgagcggtc tgatcttgaa gaacaatgtg aaagctcatt ttcagaactt cccaaatggt
      361 gtgacagact tcatcaagag tgaatgtcta aataacattg gggactcctc tccgttgatc
      421 agagccactg tcggtatttt aattacaacc atagcctcca agggagagct gcagaattgg
      481 cctgatctct taccaaaact ctgtagcctg ctggattccg aagactacaa cacttgtgag
      541 ggagccttcg gtgcccttca gaagatatgt gaggactctg cagagatttt agacagtgat
      601 gtcttagatc gccctctcaa catcatgatc cccaagtttt tacagttttt caagcacagc
      661 agtcccaaaa taaggtctca tgctgttgca tgtgtcaatc agttcatcat cagtcggacc
      721 caggcgctca tgctgcacat cgattccttc attgagaacc tctttgcact ggctggcgac
      781 gaggaagcag aggtgcggaa gaacgtgtgc cgggcgcttg tgatgttgct tgaagtccgg
      841 atggatcgcc tgcttcctca tatgcacaac atagtcgagt acatgctgca gagaacccaa
      901 gaccaagatg agaatgtagc tctggaggcc tgtgaattct ggctgacttt ggctgaacag
      961 ccaatatgca aagatgtact tgtaaggcat ctaccaaagt tgattcctgt gttagtgaat
     1021 ggcatgaagt actcagatat agatattatc ctgcttaagg gtgatgttga ggaagatgag
     1081 accatcccgg atagtgagca ggatatacgg ccgcggtttc atcgctcgag gacagtggct
     1141 cagcagcatg aggaggatgg gattgaggag gaagacgatg atgatgacga aattgatgat
     1201 gatgacacga tttctgactg gaacctgaga aagtgttctg ctgctgctct cgatgttctc
     1261 gcaaatgttt atcgtgatga gcttttgccg cacattctgc cacttttgaa agaattgctt
     1321 ttccatcatg aatgggttgt gaaagaatct ggcatcttgg ttttaggagc aattgctgaa
     1381 ggttgtatgc aaggcatgat tccgtacctg cccgagctca ttcctcacct tattcagtgc
     1441 ctttctgata aaaaggctct tgtgcgttcc atcacctgct ggactcttag ccgctatgca
     1501 cactgggtag tcagccagcc accagatacg tacctgaagc cattaatgac agaactgctg
     1561 aagcgtatcc tcgatagcaa caagagagta caggaagcag cttgcagtgc cttcgctaca
     1621 ttagaagagg aggcttgtac agagctcgtc ccttaccttg cttatatact cgataccctc
     1681 gtcttcgcct tcagtaaata ccagcataag aacctgctca ttctgtacga cgccataggg
     1741 acactggcag attcagtggg acatcattta aacaagccag aatatattca gatgctaatg
     1801 cctcctttga tccagaaatg gaacatgctg aaggatgaag acaaggatct tttccctttg
     1861 cttgagtgtc tctcgtctgt tgccacagcc ttgcagtctg gcttccttcc atattgtgaa
     1921 cctgtatatc agcgttgcgt aaacctagtt cagaagactc tggcacaagc catgctaaac
     1981 aatgctcaac cagaacagta tgaagctcca gataaagatt ttatgattgt ggctcttgac
     2041 ttactcagcg gcctggctga gggcctggga ggcaacattg agcagctggt ggcccgcagt
     2101 aacatcctga cgctgatgta ccagtgcatg caggataaaa tgcccgaggt tcggcagagt
     2161 tcttttgcat tgctaggtga cctgactaaa gcgtgcttcc agcatgttaa gccttgtata
     2221 gctgatttca tgccaatatt gggaaccaat ctaaatccag agtttatttc agtctgcaac
     2281 aatgccacct gggcgattgg ggaaatatca atccaaatgg gtatagagat gcagccctac
     2341 atccctatgg tgttgcacca gcttgtggag atcattaaca gacccaacac cccaaagacg
     2401 ctgttggaga acacagcaat aacaattggt cgtcttggtt acgtttgtcc tcaagaggtg
     2461 gcccccatgc tacagcagtt tataagaccc tggtgtacct ctctgagaaa cataagagac
     2521 aatgaagaaa aagattcagc attccgtggg atttgtacca tgatcagtgt gaatcccagt
     2581 ggcgtaatcc aagattttat atttttttgt gatgctgttg catcatggat taacccaaaa
     2641 gatgatctca gagacatgtt ctgtaagatc cttcatggat ttaaaaacca agttggggat
     2701 gaaaattgga ggcgattctc tgaccagttt cctcttccct taaaagagcg tcttgcagct
     2761 ttttatggtg tttaatctaa tacacttaag ctgcagtccc aaaactaggg gtccgtcagt
     2821 cttggagact atgggagcct ctgcacccag ggaaaatgtt accctttaca ggggggaagg
     2881 gtaaaccagt agggaataca gtacaatccc aaccctactg ggaggggcgg gagggaggtg
     2941 ttgccgtcac tgtactaagt cgatgttggg aaatgtttta acatctggag cctttgtggg
     3001 tggaaatctg tctccaatta caactctgca ctggatgtga agaagcaaaa actattccgt
     3061 ctactcacag aacagtactt tctggaatat tatgggggat tgtaccaaaa caagaaccac
     3121 ttaaacaatg cctagggaat gataaggagg aggaaaatcc caggtccaca gcaaaggaca
     3181 cctaagagtg ggaggctgca gcagtaaggt ggctttttat caaaggtttt tatataaatt
     3241 tcccacgtta cggggccttg ttttgcatgc tgatgaagaa ctacttaaaa ctaacagtta
     3301 aaatcagctt gcctccccta gtagaagcag gttcttggaa gttacaattt aaggtacccc
     3361 caaaaagttg gaaataaaag gaaacaaatt tgaacaacga agcaccctgt gacatgccga
     3421 cgcgccactc ctttatcttt gcgggctggg cagggaggga ggaataactg gggtcgggga
     3481 tcaaacgaaa ggtgacatct aatttcacat taaaatgtta gtggatataa tttcatggtt
     3541 gtacttctta gatttaattt ctaggccaac acgttatctt aaggtgcagt tttaaggttc
     3601 aggcatgcgc tctggctcat agtgtttgaa agtgatgtcc attagtggga ccgtagcatg
     3661 cttgcctcgc atagagtgca gttggtattc atttacctat gtgcgccagt ttgggttgcc
     3721 gttaccagtt caatatagcc ctgcatttaa aattcctttt aagattcgtg gattttattt
     3781 ttattaagta tatagatata taaagtactg tagtttacag ctaggccttg agatatcttt
     3841 ttaggatctg ttaggaataa gattgatatt gtattgtgtg taacctgcac aatgtggaaa
     3901 gctgatgaac ctgtgcctcc gtgctgtcag tgtggtgtgc tttctgcatg gttatatact
     3961 actagtaact ttatcaaatt tcaaattaca tggtaaaaga tctgtaaagg ctgcatccat
     4021 gttagttggc acataaaggc aattaattgt agaagttgaa acatgatcgc tatatttcaa
     4081 tgtttattcc cgttcaacgt actgcctcta agctttcctt taatttcttt atttttattt
     4141 ttgttcgaag catgatctta aagctatgtt taagttaatg gatgtaatgc agggttccta
     4201 cactgtattg gcgcatgttg gtggccctct gtgccctaag catatgcaca tggggcacat
     4261 acacagctac agagtgagag ttggtttgga tcctcttcat ttcatttgtt tgcctttctg
     4321 ttatttttcc ctctgcttct atgtatctct gtgaataaat ccttgttaag ttaacccttt
     4381 gctcttcctc ccatgtgtat tttcttacat actgtgaatg tgaagaccct aactggtaca
     4441 cttgatctta tgtccatatg aaagtgcaaa tctttattaa tttggattgc ctgagcagtg
     4501 tgtatcccat gatgatgaag ggaaatggag agatttttcc ttttagctct gctggtcaga
     4561 gattaagcca cacctttcca tttttcaatg ctgcatattt aatctgcaaa aaaaagttaa
     4621 gccataacag cctttttata ttttaatttg tcacctttgc attgcagaat aaatacccaa
     4681 taaccgtttt tataggagtt gctcctcttt gcatggttct gttctataga agtgactgat
     4741 aacagccatt gcactgaagt gtgagctgtc cgtgtgtgac tttataaata cagttttaaa
     4801 acatttcaca tgcatggtag cacgcagagg gttttgttgt ggatgtgttt gtttgtttgt
     4861 ttgtttgttt taacatgttc ttttactttc ttttcttctg ttttggcttt gcaaatttca
     4921 gtctgtgggg cctgaaataa aaccatgtat agtgcccagt ctctctctcc tattttttat
     4981 ttttttaagc tagactcaag ggtgtgtggt atttcgcttt ttagtacatt actttactgg
     5041 aagcttaaaa tttgcttctc agttggcctt tagagcaaga gtggaatagc tgttttattc
     5101 ttaggttgta gaatgccttg tagtgcttca tatactttgg ggacgtaata taggagatag
     5161 tcttacataa gtaatataga aacagccaca tttgtccatt aatgcataag aagcaaagta
     5221 aagttttctg gatctgtgat aaacaattct ttgttaatta aaggcaattt tattacctgt
     5281 ctaattaaga atatatttcc aagttagcag ttatgcagct caaaagcagt atttggcttt
     5341 atagtaaatt tgtttctaaa tctaataact ttaaaataga aacttaagta aatatggctt
     5401 acaccctaac actggggtgg ttttcttttc taatgtagct ttccctgttg aggctattgt
     5461 ttcttcttaa tttatattat attttaaaca acatagtata tagcacagga atttgcagaa
     5521 gccatttaag ttttttgagg tcagcgctga gttagcattt attgttttga tgaaacgtga
     5581 cacatcatgg gatatatata tatatataaa gaacttaatt cttgtggtgc agtcttgata
     5641 gttttgaatg ttgcttaaat gtctatgagg ttgcgagttt gtcattgtta cattccagtg
     5701 tttctgcctc ttggcatgct taaagcacgg cttacttcat ctgctcctta cacactaaaa
     5761 tgctcttagt gtgctcaact acagagatag ccgctgctga gtgatgtaga ttttctactt
     5821 gaatattttt atgttgtagg aacctcagga ggtcagtgtt tactgtttta tatatgcctt
     5881 ctttttcctg tttgagcttc tctctctgaa ggattctaac agaacaaaag ctgctgatca
     5941 acctaagttg gaaacagaaa gtgtgtctag tgtcattgaa gtgcgtgctt ggcagttcca
     6001 tgttacctac cacccattag ttagcaagcg aacttagtgc cacaggtgtt catttactcg
     6061 ggaaatggtt ttgctttttg tattgtctaa tctctttaat gacttcatac tgtgtaagga
     6121 aaattacttt tgtattgttt gttggttatt tccaaaataa ccaccttgta aataccctta
     6181 gagccagccc tgatgcttta catcattgct acttgatttt gttatgcaac tcagatctca
     6241 gatctcagaa tacttaacca agccattaca tcttaaaaca aaacaagtag catacatttt
     6301 gtaattaaca ttgataaaca ctgtgatttt tttggttaaa actttttcat tgatctgaat
     6361 tgcttaaatt gcatatattg taaaatagag tcagatgtat attttaaaat aatttcagct
     6421 gactttcctc tgactgcttt gtctactcag ttagaaatat tcagatacaa gttttatctc
     6481 aggtgaatat ccttgtatcg ttttgctttg ctgacatgtt tataaagaaa gaccatcata
     6541 cttaatagtc tttggaggtt tgtaaggtgt ttttttgttt tgttttaatg tattttattg
     6601 attatcttcc cggttagtcg attatttttc ctcctccaat tgttttacca gaccagagag
     6661 agaaataaaa acctctcctc acacttggta tggagactcg actgaaaatg agttgagttt
     6721 ggtcctctcc ttttccaaac actcagaaca caaaagtgga atcttctccc tacataaaat
     6781 tttgcaaagt tggggtgcca gaattaaatt atgtattaaa ctcttgtctc catttaacac
     6841 ctttgcagtg aaaaactttt tgcttcatat agttaattat attcatattg aatgtattaa
     6901 cagatagcag tgcaaaagca ttcttcccaa gagaagagtg taccatgcac gtctgcaccc
     6961 caggcccttc ctgagtcttc aggtgcacac agccttcttg tggagctact tttgcaaata
     7021 ttacaaggtg gggggacact aattgttgct gctagtgctg tctaataacg tggtcattgg
     7081 aatctaagga aactagggtc tgtggttaac accctgggat aattgtgatg ggcagatgcg
     7141 cagtaatggc ctgtgttggt aacaatatta tctagttcaa tgccatgcag tagcattgta
     7201 tagcaactag tgcatttcat actgtattaa atatggcact gcttctaagc ctcgttccag
     7261 ttaagtttca catgacagtt agctcctctg acctgtcttt ctaggtaact ggaaatgaac
     7321 ttcaggcttg atacagatct tctccctgtt ggtattacat atatagagat aattctcaat
     7381 gcctaattaa tatacttata catttgttaa ggtatgcgca taattacagc aactgttttt
     7441 atagtttgta tcccagagta tactgtgttg aaataagaag atgcatagca gctgaatgca
     7501 tgcatgactt tgagggaagt taagactggt tttcttttcc catctctgca cattgaaaaa
     7561 ccaagccaag tctatttgcc aatgatagat cactaattac aaagctagta gttctccttt
     7621 gggtaagagt ctgtttgcca tacatcactc ttccactgtg gcttggtatt gtgcagatta
     7681 cctctttgat aggtctagtc accttgttca gtgcctgtct aacatggcta gctacaaggc
     7741 aatgttgtct cctcctaatg tccacctcag tgacgaggtt ccacactgtc tcctcacctc
     7801 ttgtcacggc ttccctatgt ggatatataa agaaccaaac tgattcgctg ttgttttttt
     7861 tttttttatg gagataataa ttagataata ctatgtaatt aattttactc agtagattat
     7921 catcttaaga gatgcttaaa catggtaaat cacttcatat tatgaaagtt ttacacttag
     7981 tattcatgtt aacattgggt gcaataactt agtagtactt gcttagttat aaataactgg
     8041 atctttctgc tgacaactta ggttgtatga gttatgctta aaagctttaa atcggatgtt
     8101 tcctgtacct gccacactat gttagaatgt gtccttcaac catatcctgc aacttctcaa
     8161 actgtactaa attgatattt cttgaagtct aactctgtgc taacagatct tcattttaaa
     8221 tagaatacgg ttttaatttt tgataagctg ctgaatttta aagagagttt tttggggcca
     8281 ccaaatattt tggatcatgc agagaatata tattgtactg tagtaatttt gtatttacat
     8341 ttgtatgatg tgacataata gatgtgaatg ttaatcactg cttgactatg ttaataaagt
     8401 tgtttaacta ta
//
