LOCUS       NM_009405                724 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus troponin I, skeletal, fast 2 (Tnni2), mRNA.
ACCESSION   NM_009405 XM_006508539
VERSION     NM_009405.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 724)
  AUTHORS   Montanes-Agudo,P., Aufiero,S., Schepers,E.N., van der Made,I.,
            Cocera-Ortega,L., Ernault,A.C., Richard,S., Kuster,D.W.D.,
            Christoffels,V.M., Pinto,Y.M. and Creemers,E.E.
  TITLE     The RNA-binding protein QKI governs a muscle-specific alternative
            splicing program that shapes the contractile function of
            cardiomyocytes
  JOURNAL   Cardiovasc Res 119 (5), 1161-1174 (2023)
   PUBMED   36627242
REFERENCE   3  (bases 1 to 724)
  AUTHORS   Gao,M., Liu,X., Guo,P., Wang,J., Li,J., Wang,W., Stoddart,M.J.,
            Grad,S., Li,Z., Wu,H., Li,B., He,Z., Zhou,G., Liu,S., Zhu,W.,
            Chen,D., Zou,X. and Zhou,Z.
  TITLE     Deciphering postnatal limb development at single-cell resolution
  JOURNAL   iScience 26 (1), 105808 (2022)
   PUBMED   36619982
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 724)
  AUTHORS   Wu,T., Liang,Z., Zhang,Z., Liu,C., Zhang,L., Gu,Y., Peterson,K.L.,
            Evans,S.M., Fu,X.D. and Chen,J.
  TITLE     PRDM16 Is a Compact Myocardium-Enriched Transcription Factor
            Required to Maintain Compact Myocardial Cardiomyocyte Identity in
            Left Ventricle
  JOURNAL   Circulation 145 (8), 586-602 (2022)
   PUBMED   34915728
REFERENCE   5  (bases 1 to 724)
  AUTHORS   Tan,C., Zhu,S., Chen,Z., Liu,C., Li,Y.E., Zhu,M., Zhang,Z.,
            Zhang,Z., Zhang,L., Gu,Y., Liang,Z., Boyer,T.G., Ouyang,K.,
            Evans,S.M. and Fang,X.
  TITLE     Mediator complex proximal Tail subunit MED30 is critical for
            Mediator core stability and cardiomyocyte transcriptional network
  JOURNAL   PLoS Genet 17 (9), e1009785 (2021)
   PUBMED   34506481
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 724)
  AUTHORS   Guenet,J.L., Simon-Chazottes,D., Gravel,M., Hastings,K.E. and
            Schiaffino,S.
  TITLE     Cardiac and skeletal muscle troponin I isoforms are encoded by a
            dispersed gene family on mouse chromosomes 1 and 7
  JOURNAL   Mamm Genome 7 (1), 13-15 (1996)
   PUBMED   8903721
REFERENCE   7  (bases 1 to 724)
  AUTHORS   Zhu,L., Lyons,G.E., Juhasz,O., Joya,J.E., Hardeman,E.C. and Wade,R.
  TITLE     Developmental regulation of troponin I isoform genes in striated
            muscles of transgenic mice
  JOURNAL   Dev Biol 169 (2), 487-503 (1995)
   PUBMED   7781893
REFERENCE   8  (bases 1 to 724)
  AUTHORS   Braun,T. and Arnold,H.H.
  TITLE     Inactivation of Myf-6 and Myf-5 genes in mice leads to alterations
            in skeletal muscle development
  JOURNAL   EMBO J 14 (6), 1176-1186 (1995)
   PUBMED   7720708
REFERENCE   9  (bases 1 to 724)
  AUTHORS   Chen,Z., Friedrich,G.A. and Soriano,P.
  TITLE     Transcriptional enhancer factor 1 disruption by a retroviral gene
            trap leads to heart defects and embryonic lethality in mice
  JOURNAL   Genes Dev 8 (19), 2293-2301 (1994)
   PUBMED   7958896
REFERENCE   10 (bases 1 to 724)
  AUTHORS   Koppe,R.I., Hallauer,P.L., Karpati,G. and Hastings,K.E.
  TITLE     cDNA clone and expression analysis of rodent fast and slow skeletal
            muscle troponin I mRNAs
  JOURNAL   J Biol Chem 264 (24), 14327-14333 (1989)
   PUBMED   2760067
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL603651.14.
            
            On or before Jun 5, 2018 this sequence version replaced
            XM_006508539.3, NM_009405.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CB575219.1, CB588547.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                AL603651.14        93525-93577         c
            54-83               AL603651.14        92895-92924         c
            84-90               AL603651.14        92696-92702         c
            91-132              AL603651.14        92181-92222         c
            133-261             AL603651.14        91962-92090         c
            262-351             AL603651.14        91733-91822         c
            352-528             AL603651.14        91311-91487         c
            529-724             AL603651.14        90983-91178         c
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 87.93 cM"
     gene            1..724
                     /gene="Tnni2"
                     /note="troponin I, skeletal, fast 2"
                     /db_xref="GeneID:21953"
                     /db_xref="MGI:MGI:105070"
     exon            1..53
                     /gene="Tnni2"
                     /inference="alignment:Splign:2.1.0"
     exon            54..83
                     /gene="Tnni2"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..624
                     /gene="Tnni2"
                     /codon_start=1
                     /product="troponin I, fast skeletal muscle"
                     /protein_id="NP_033431.1"
                     /db_xref="CCDS:CCDS40192.1"
                     /db_xref="GeneID:21953"
                     /db_xref="MGI:MGI:105070"
                     /translation="MGDEEKRNRAITARRQHLKSVMLQIAATELEKEESRRESEKENY
                     LSEHCPPLHIPGSMSEVQELCKQLHAKIDVAEEEKYDMEVKVQKSSKELEDMNQKLFD
                     LRGKFKRPPLRRVRMSADAMLKALLGSKHKVCMDLRANLKQVKKEDTEKERDLRDVGD
                     WRKNIEEKSGMEGRKKMFESES"
     exon            84..90
                     /gene="Tnni2"
                     /inference="alignment:Splign:2.1.0"
     exon            91..132
                     /gene="Tnni2"
                     /inference="alignment:Splign:2.1.0"
     exon            133..261
                     /gene="Tnni2"
                     /inference="alignment:Splign:2.1.0"
     exon            262..351
                     /gene="Tnni2"
                     /inference="alignment:Splign:2.1.0"
     exon            352..528
                     /gene="Tnni2"
                     /inference="alignment:Splign:2.1.0"
     exon            529..724
                     /gene="Tnni2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      701..706
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tnni2"
                     /note="hexamer: AATAAA"
     polyA_site      724
                     /gene="Tnni2"
                     /note="major polyA site"
ORIGIN      
        1 aactcactgc tgccggcctg acctcgctcc cagcactgct gcacagcatc taggatcaga
       61 gcttgagatc tcaggatggg agatgaggag aagcgcaaca gggccatcac ggcccggaga
      121 cagcacctga agagtgtgat gctccagata gcggccacag agctggagaa agaggaaagc
      181 cgccgagaat ctgagaagga gaactacctg tcagaacact gcccgccact gcacatccca
      241 ggctccatgt ctgaagtgca ggaactctgc aaacaactgc atgcgaagat cgacgtggct
      301 gaagaggaga aatatgacat ggaggtgaag gtgcagaaga gcagcaagga gctggaagac
      361 atgaaccaga agctatttga cctgaggggc aagttcaaga ggcccccact tcggagggtg
      421 cgtatgtctg ccgacgccat gctgaaggcg ctgctgggct ctaagcacaa ggtgtgcatg
      481 gacctgaggg ccaacctgaa gcaggtcaag aaggaagaca ctgagaagga acgggacctg
      541 cgtgacgtgg gtgactggag gaagaatatt gaggagaagt ctggcatgga gggccggaag
      601 aaaatgttcg agtctgagtc ctaactgccc acttccccag taccacccta gcattgggga
      661 agcacccaac atccagtctg ggagtacttg gcctcgctcc aataaaggat tcacatctct
      721 ttca
//
