LOCUS       NM_026910               7146 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus TRAF2 and NCK interacting kinase (Tnik), transcript
            variant 1, mRNA.
ACCESSION   NM_026910 XM_001473602 XM_001473621 XM_001474897 XM_001474909
VERSION     NM_026910.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Pham,T.C.P., Dollet,L., Ali,M.S., Raun,S.H., Moller,L.L.V.,
            Jafari,A., Ditzel,N., Andersen,N.R., Fritzen,A.M.,
            Gerhart-Hines,Z., Kiens,B., Suomalainen,A., Simpson,S.J., Salling
            Olsen,M., Kieser,A., Schjerling,P., Nieminen,A.I., Richter,E.A.,
            Havula,E. and Sylow,L.
  TITLE     TNIK is a conserved regulator of glucose and lipid metabolism in
            obesity
  JOURNAL   Sci Adv 9 (32), eadf7119 (2023)
   PUBMED   37556547
  REMARK    GeneRIF: TNIK is a conserved regulator of glucose and lipid
            metabolism in obesity.
REFERENCE   3  (bases 1 to 7146)
  AUTHORS   Buchl,S.C., Hanquier,Z., Haak,A.J., Thomason,Y.M., Huebert,R.C.,
            Shah,V.H. and Maiers,J.L.
  TITLE     Traf2 and NCK Interacting Kinase Is a Critical Regulator of
            Procollagen I Trafficking and Hepatic Fibrogenesis in Mice
  JOURNAL   Hepatol Commun 6 (3), 593-609 (2022)
   PUBMED   34677004
  REMARK    GeneRIF: Traf2 and NCK Interacting Kinase Is a Critical Regulator
            of Procollagen I Trafficking and Hepatic Fibrogenesis in Mice.
REFERENCE   4  (bases 1 to 7146)
  AUTHORS   Walczyk-Mooradally,A., Holborn,J., Singh,K., Tyler,M., Patnaik,D.,
            Wesseling,H., Brandon,N.J., Steen,J., Graether,S.P., Haggarty,S.J.
            and Lalonde,J.
  TITLE     Phosphorylation-dependent control of Activity-regulated
            cytoskeleton-associated protein (Arc) protein by TNIK
  JOURNAL   J Neurochem 158 (5), 1058-1073 (2021)
   PUBMED   34077555
  REMARK    GeneRIF: Phosphorylation-dependent control of Activity-regulated
            cytoskeleton-associated protein (Arc) protein by TNIK.
REFERENCE   5  (bases 1 to 7146)
  AUTHORS   Jaeger-Ruckstuhl,C.A., Hinterbrandner,M., Hopner,S., Correnti,C.E.,
            Luthi,U., Friedli,O., Freigang,S., Al Sayed,M.F., Buhrer,E.D.,
            Amrein,M.A., Schurch,C.M., Radpour,R., Riether,C. and
            Ochsenbein,A.F.
  TITLE     TNIK signaling imprints CD8+ T cell memory formation early after
            priming
  JOURNAL   Nat Commun 11 (1), 1632 (2020)
   PUBMED   32242021
  REMARK    GeneRIF: TNIK is an important regulator of effector and memory T
            cell differentiation and induces a population of stem cell-like
            memory T cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7146)
  AUTHORS   Kawabe,H., Neeb,A., Dimova,K., Young,S.M. Jr., Takeda,M.,
            Katsurabayashi,S., Mitkovski,M., Malakhova,O.A., Zhang,D.E.,
            Umikawa,M., Kariya,K., Goebbels,S., Nave,K.A., Rosenmund,C.,
            Jahn,O., Rhee,J. and Brose,N.
  TITLE     Regulation of Rap2A by the ubiquitin ligase Nedd4-1 controls
            neurite development
  JOURNAL   Neuron 65 (3), 358-372 (2010)
   PUBMED   20159449
REFERENCE   7  (bases 1 to 7146)
  AUTHORS   Mahmoudi,T., Li,V.S., Ng,S.S., Taouatas,N., Vries,R.G.,
            Mohammed,S., Heck,A.J. and Clevers,H.
  TITLE     The kinase TNIK is an essential activator of Wnt target genes
  JOURNAL   EMBO J 28 (21), 3329-3340 (2009)
   PUBMED   19816403
  REMARK    GeneRIF: siRNA depletion of TNIK followed by expression array
            analysis showed that TNIK is an essential, specific activator of
            Wnt transcriptional programme.
REFERENCE   8  (bases 1 to 7146)
  AUTHORS   Uechi,Y., Bayarjargal,M., Umikawa,M., Oshiro,M., Takei,K.,
            Yamashiro,Y., Asato,T., Endo,S., Misaki,R., Taguchi,T. and
            Kariya,K.
  TITLE     Rap2 function requires palmitoylation and recycling endosome
            localization
  JOURNAL   Biochem Biophys Res Commun 378 (4), 732-737 (2009)
   PUBMED   19061864
REFERENCE   9  (bases 1 to 7146)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   10 (bases 1 to 7146)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC050868.1, CK620825.1 and CD348964.1.
            
            On or before Jul 8, 2009 this sequence version replaced
            XM_001474897.1, XM_001474909.1, XM_001473602.1, XM_001473621.1.
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC137799.1, SRR7345562.254780.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849390, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1068              BC050868.1         1-1068
            1069-1761           BC050868.1         1173-1865
            1762-1962           CK620825.1         414-614
            1963-2811           BC050868.1         1980-2828
            2812-3134           CD348964.1         387-709
            3135-7146           BC050868.1         3128-7139
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 11.92 cM"
     gene            1..7146
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /note="TRAF2 and NCK interacting kinase"
                     /db_xref="GeneID:665113"
                     /db_xref="MGI:MGI:1916264"
     exon            1..486
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             430..4512
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1; traf2
                     and NCK-interacting protein kinase"
                     /codon_start=1
                     /product="traf2 and NCK-interacting protein kinase isoform
                     1"
                     /protein_id="NP_081186.1"
                     /db_xref="CCDS:CCDS50877.1"
                     /db_xref="GeneID:665113"
                     /db_xref="MGI:MGI:1916264"
                     /translation="MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRH
                     VKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLW
                     LVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVL
                     LTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWS
                     LGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQ
                     RPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEEND
                     SGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQ
                     KRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQ
                     LEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQ
                     EQRPLEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPP
                     RSESFSISGVQPARTPPMLRPVDPQIPQLVAVKSQGPALTASQSVHEQPTKGLSGFQE
                     ALNVTSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPA
                     LARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESSLQRTSSGSSSSSSTPSSQP
                     SSQGGSQPGSQAGSSERSRVRANSKSEGSPVLPHEPSKVKPEESRDITRPSRPASYKK
                     AIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSEEEEEDGESETHDGTVA
                     VSDIPRLIPTGAPGNNEQYNMGMVGTHGLETSHADTFGGSISREGTLMIRETAEEKKR
                     SGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHSQEMDSGAEYGIGSSTKAS
                     FTPFVDPRVYQTSPTDEDEEDDESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNI
                     RPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRF
                     QQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIH
                     YKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQR
                     LKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEG
                     VYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKR
                     AQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW"
     exon            487..552
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            553..609
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            610..735
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            736..846
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            847..937
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1068
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1123
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1202
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1378
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1445
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1650
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1761
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1848
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1849..2037
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2038..2202
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..2442
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2443..2549
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2550..2713
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2714..2811
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2812..2835
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2836..3020
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3021..3150
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3151..3315
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3316..3427
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3428..3598
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3599..3742
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3743..3877
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3878..3978
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3979..4128
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4129..4288
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4289..4428
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4429..7115
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7090..7095
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /note="hexamer: AATAAA"
     polyA_site      7115
                     /gene="Tnik"
                     /gene_synonym="1500031A17Rik; 4831440I19Rik;
                     C530008O15Rik; C630040K21Rik"
                     /note="major polyA site"
ORIGIN      
        1 ctcccaggaa gcggtgatag acagttctgt gtggttctcc acccccacct cccagctgag
       61 tggaggagtt gatatgtctc attataacag ccaatgcagc cgccatccac gcacaagcaa
      121 agaagcatgt cccatttaaa tacacccacg cagccccagc gcccttagcc gatcagggcg
      181 cgcagcccac acgcaccgcg gctccgggct tggagatccg cgcaggtcgg gctccgggat
      241 cccgcggatc gacgctctcg gaagatcggg atcggactct tgctggggcc cgggtgggcg
      301 ggggaggctg ggccccgggc ctctctggcg gacagccgca tgaggacgcg agcgaaatag
      361 accggggtgg aattgccaag gaagacgctt cggggtggtt ttggtccatt tctcccgcga
      421 agagtcgaca tggcgagcga ctccccagct cgcagcctgg atgaaatcga tctctccgcc
      481 ctgagggacc ctgcagggat ctttgagttg gtggaacttg tcggaaatgg cacgtatggt
      541 caagtttata agggtcgtca tgtcaaaacg ggccagcttg ctgccattaa ggttatggat
      601 gtcacagggg atgaagagga agaaatcaaa caagaaatta acatgttgaa gaaatattct
      661 catcacagga acattgctac atactacggt gcttttatca aaaagaaccc tcctggcatg
      721 gatgaccaac tctggttggt tatggagttc tgtggtgctg gctctgtcac tgacctgatc
      781 aagaacacga aaggcaacac attgaaagag gagtggattg catacatctg cagggagatc
      841 ttacggggcc tgagtcacct gcaccagcac aaagtgattc atcgagatat caaagggcag
      901 aacgtcttgt tgactgaaaa tgcagaggtt aagctagtgg attttggagt gagtgcccag
      961 cttgaccgaa ctgtgggcag gaggaacacg ttcatcggga ctccctactg gatggcacca
     1021 gaagtcattg cctgtgatga gaacccggat gccacatatg atttcaagag tgacttgtgg
     1081 tctttgggaa tcaccgccat tgagatggca gaaggtgccc cccccctctg tgacatgcat
     1141 cccatgagag ccctcttcct catcccacgg aaccctgcac ctcggctcaa gtctaagaag
     1201 tggtcaaaaa aattccagtc atttatcgag agctgcttgg taaagaatca cagccagcgg
     1261 ccagccacgg agcagttgat gaagcaccca ttcatacgag accaacctaa tgagaggcag
     1321 gtccgcatcc agctgaagga ccacattgat cgaacaaaga agaagcgagg agaaaaagat
     1381 gagactgagt atgaatacag cggaagtgag gaagaagagg aagagaatga ctctggggaa
     1441 cccagctcca ttctgaacct accaggggag tcaacactgc gaagggactt cctgagactg
     1501 cagctggcca acaaggagcg ctcagaggcc ctgcggcgcc aacagctgga gcagcagcag
     1561 cgggagaatg aagaacacaa gcggcagcta ctggctgagc gccagaagcg catcgaagag
     1621 cagaaggagc aaaggcggag gctggaggag caacaaaggc gagaaaaaga gcttcggaaa
     1681 cagcaggagc gggaacagcg ccggcactac gaagaacaga tgcgtcggga ggaggagagg
     1741 aggcgtgccg aacatgagca ggaatacatc aggcggcagc tagaagaaga gcaaaggcag
     1801 ttagagatct tacagcagca gctactgcat gagcaagctc tacttctgga atataagcgc
     1861 aaacaattgg aagaacagag acaagcagaa agactgcaga ggcagctaaa gcaagagcgg
     1921 gactatctgg tttccctcca gcatcagcgg caggagcaga ggcccctgga gaagaagcca
     1981 ctgtaccatt acaaggaggg catgagtcct agtgagaagc cggcctgggc caaggaggta
     2041 gaagaacgct caagactcaa ccgacagagt tcacctgcca tgcctcacaa ggttgccaac
     2101 aggatctcgg accccaacct gcccccaaga tcagagtcct tcagcattag tggggttcag
     2161 cctgcaagga cacccccaat gctcagacct gttgaccccc agatcccgca gctggtagct
     2221 gtcaaatccc agggacctgc cttgacggcc tcccagtcag tacatgagca acccacaaag
     2281 ggcctgtctg ggttccagga ggctctgaat gtgacctctc accgggtcga gatgccacgc
     2341 cagaactcgg atcccacctc agaaaaccct cctctcccca cgagaattga gaagtttgac
     2401 agaagctctt ggttacgaca ggaagaagac attccaccaa aggtgcctca aagaacaact
     2461 tctatatccc cagcactagc cagaaagaat tcccctggca atggcagtgc tctgggcccc
     2521 agacttggat ctcagcccat cagagcaagc aaccctgatc tgcgcaggac agagccagtc
     2581 ctggagagtt ccctgcagcg gacaagcagt ggcagttcct ccagctccag cactcccagc
     2641 tcccagccca gctcccaagg aggctctcaa cctggctccc aagcaggatc tagtgagcgg
     2701 tccagagtgc gggccaacag taagtccgaa ggatcacccg tgctccccca tgagccttcc
     2761 aaggtgaaac cagaagaatc cagagacatc acacggccca gtcggccagc tagctataag
     2821 aaagctatag atgaggatct gacggcatta gccaaagaat tacgagaact ccgcattgaa
     2881 gaaacaaacc gccccctgaa gaaagtgact gattactctt cctccagcga ggagtcggag
     2941 agcagtgagg aagaagagga agatggagag agtgagacac atgacgggac ggtggctgtc
     3001 agtgacatac ccagactaat acccaccgga gctccaggga acaatgagca gtacaacatg
     3061 gggatggtcg ggacacatgg gctggaaact tcgcatgcgg acacctttgg cggcagcatt
     3121 tcaagagaag gaaccttgat gatcagagag acggctgaag agaagaagcg atctggccac
     3181 agtgacagta atggattcgc cggtcacatc aatctcccag accttgtaca gcagagccat
     3241 tcgccagctg gaactcccac tgaggggctg ggccgcgtct ccactcattc ccaggagatg
     3301 gactctgggg ctgaatatgg tatagggagc agcaccaaag cctctttcac ccccttcgtg
     3361 gaccctcgag tgtaccagac atcgcccact gatgaagatg aagaggatga tgagtcttca
     3421 gctgctgccc tgtttactag cgaacttctt aggcaagaac aggccaaact caatgaagcg
     3481 aggaagattt cagtggtaaa tgtgaaccca acaaacattc gccctcatag tgacacaccg
     3541 gaaatcagaa aatacaagaa acgcttcaat tcagaaatac tttgtgcagc tctatggggt
     3601 gtgaaccttc tggtggggac tgaaaatggc ctgatgcttt tggacagaag tggccaaggc
     3661 aaagtctaca acctaatcaa ccggaggcgg tttcagcaga tggatgtgct agaaggacta
     3721 aatgttcttg tcacgatatc aggaaagaag aacaagctcc gtgtgtacta tctctcatgg
     3781 ttaagaaaca gaatcctgca caatgaccca gaagtggaaa agaagcaggg ctggatcact
     3841 gtcggtgact tggaaggctg catccattac aaagtcgtta aatatgaaag aatcaagttc
     3901 ctggtgattg ccttaaagaa tgcagtagag atatatgcgt gggcccctaa accttaccat
     3961 aagttcatgg catttaagtc ttttgcagat cttcagcata agcctctgct cgttgacctc
     4021 acagtagaag aaggtcaaag gttaaaggtc atatttggct cacacactgg tttccatgta
     4081 attgatgttg attctggaaa ctcctacgat atctatatac catcccatat tcagggcaat
     4141 atcactcctc atgctatcgt catcttgcct aaaacagatg gaatggagat gcttgtctgc
     4201 tatgaggatg aaggggtgta cgtgaacacc tacggccgga tcactaagga tgtggtgctc
     4261 caatggggag aaatgcccac atctgtggcc tacattcatt ccaatcagat aatgggctgg
     4321 ggcgagaaag ctattgagat ccggtcagtg gaaacaggac atttggatgg agtgtttatg
     4381 cataaacgag ctcaaaggtt aaagtttcta tgtgaaagaa atgataaggt attttttgca
     4441 tccgtgcgat ctggaggaag tagccaagtg tttttcatga ccctcaacag aaattccatg
     4501 atgaactggt aacagaagag cacttggcac ttatcttcat ggcgttattt ctaatgtaaa
     4561 agaacataac tcatgtggac gtatgccagt ctagaggcag aatcagaagg cctggttgaa
     4621 catactgctc tctccgtttc ctctccctcc atctctccca atatagtcct ttttggtgtt
     4681 gcagcctggt tgagaaggag agaaagaggt ggcgggaatg gctgggcaag cgattccgag
     4741 gatgctgcat tgtctgtgga cagagatgga ccttgtgccc ttcggagtta gggatagaga
     4801 caaatattgt gtgctcttat gattaagctg tgttcatgct ggcttttcag ttctttttgg
     4861 tttggaactt tttaactttt tccccccttt accaccttac tttgtaagaa tggggggaaa
     4921 tgcatactgg gaacatagtc tttttaaaat tctgtctgcc tgctagcttt aagtatatgg
     4981 tatgttgtaa aagttccaac tcccagtagt gagaaacatc gcaataaatg gaccctatct
     5041 ccctgcactg aaggccataa ccacatagtg gggtaattta agaattttct tgccttcact
     5101 aacccaagag gctatttttg ttttgtcttg ttttgtttta ttttatcttt tttttttaat
     5161 tgctactggc taatgaattt ttgaaagaga agcaaaatag ctggttttct cctctctggg
     5221 gaaatagatt ttaaatggct aaactattag ccttaaccta ataaaatcaa ctaccactct
     5281 tgtgagtctg tcaggccata ttttgatatg gccctttctg gaatggaggg tgttgaatgg
     5341 gacagcgatg ccattgagtt gagtgggctt gtgactgggg agggactctg ggatgtgact
     5401 gtccctcaat tattatgcaa cagatcaggg caaaagatgg gatgacaggt gggtcaccca
     5461 gaaggagctt ctgagaaatg agctaatatg tctatgtaaa tacacacatg ttctttcttc
     5521 aaggcacaaa gcctccttga taagaggtcc cttcagttgg gctttgttag gactgtgact
     5581 ggttgagatc tttcccagag ctgggggtac ataacagcca tgctctggaa accctcccaa
     5641 gggtgccact ttctcaacag aaaaactgtg atggagaaga attactaata aatgttcctg
     5701 agctgcctgt gttcttactg ggtcgaggga ctaactagac ttgcaaggct gccgcacaca
     5761 gcaggcacca aaagctcaga ttttagggaa cctaaaggca aggctttgac aaaattctaa
     5821 gactctaatc atgttatgtt cctccaaact ttccaacata ctgttaacaa catctgtgca
     5881 gagatgtgta tgtatttagt tcaggttgac ttgtgtcctt atagaaaccc ttactcgaat
     5941 gatttgaacc tttatgtgac tgactgggat tttcctcaaa gctataagca tggccgccta
     6001 cggtatccag gtgttgcgaa atggtatctg tgctgtgctt cctgttttaa cctacctcgt
     6061 tttgtttgtt tttgtttctc tgttcatcac agcagtgtta tctccaggag acatatagag
     6121 agctcacctg gcaatctcag ctgtattgaa tattttcaaa acagtagttg accaaattgt
     6181 tctttagaaa ttggaggggg aaaaaaaatc tccaatgaca aaaacctaat gcaggttgtt
     6241 tttaaagaat acaattactg taaatggatc aaaaaagaaa gacaaaaact gggattctcc
     6301 tgattttgcc taaagaaatg agcagctgat gtactattaa tgagaacgaa acttatatta
     6361 ggaaaacggc acctttttaa tctggtagtt ggccaaaggg gatgagaaag gggactattc
     6421 tgagttctgg actaggggaa aatctgtaat ctccacaggt gattattgtt tgcagttatc
     6481 tgaagcaggt aacacacaga aatatagtca gggtggtctt caagtgatca ccaggatgcg
     6541 cccgtcatgg tccctctagc tctcaaaggc aatgaaatcc ttccgttctc atttttactg
     6601 ctggggttat gctgccaaac aacattgtcc tcatgaattc catgggacaa attcagcaat
     6661 agatctgggt tgaatttagc aactaccata attggatgct gatatggaca aaaataatgt
     6721 agatttgggt cttgtctcca aatgcgattg ccaccagctc tttatattgc tgctgtgatg
     6781 ttttgaacct gaggcttcct taaattatgt tgcaaactga tctttgtttt aatgttttgt
     6841 ttcgtttcat tatttctaag cgatacatct gaaacacaca gctttaaatg attttttatt
     6901 gtgggacttt gggtacagtt aagaaataaa agggaatcat tgtgtttaaa cataaggtag
     6961 tttgtgaatg tattttttaa aatctagatt aattggaaca agaaaatcat aagaaaacat
     7021 attaatgccg tcttgtttac agtatgtaca gtgacataac attacatgag ctttttctgg
     7081 tgccaacaaa ataaaacaca gacgttaaac attgaaaaaa aaaaaaaaaa aaaaaaaaaa
     7141 aaaaaa
//
