LOCUS       NM_177368               5701 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus transmembrane and tetratricopeptide repeat containing
            2 (Tmtc2), mRNA.
ACCESSION   NM_177368 NM_025775
VERSION     NM_177368.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB652678.1, AK052344.1 and BC092226.1.
            
            On Apr 20, 2006 this sequence version replaced NM_177368.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC092226.1, BC138362.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-527               BB652678.1         1-527               c
            528-2882            AK052344.1         501-2855
            2883-5701           BC092226.1         2839-5657
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 54.72 cM"
     gene            1..5701
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /note="transmembrane and tetratricopeptide repeat
                     containing 2"
                     /db_xref="GeneID:278279"
                     /db_xref="MGI:MGI:1914057"
     exon            1..813
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             731..3241
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /EC_number="2.4.1.109"
                     /note="transmembrane and TPR repeat-containing protein 2;
                     protein O-mannosyl-transferase TMTC2; transmembrane
                     O-mannosyltransferase targeting cadherins 2"
                     /codon_start=1
                     /product="protein O-mannosyl-transferase TMTC2 precursor"
                     /protein_id="NP_796342.2"
                     /db_xref="CCDS:CCDS24157.1"
                     /db_xref="GeneID:278279"
                     /db_xref="MGI:MGI:1914057"
                     /translation="MIAELVSSALGLALYLNTLSADFCYDDSRAIKTNQDLLPETPWT
                     HIFYNDFWGTLLTHSGSHKSYRPLCTLSFRLNHAIGGLNPWSYHLVNVLLHAAVTGLF
                     TRFSKALLGDGYWTFMAGLMFASHPIHTEAVAGIVGRADVGASLFFLLSLLCYIKHCS
                     TRGYSARTWGWFLGTGLCAGCSMLWKEQGVTVLAVSAVYDVFVFHRLKMKQILPTIYK
                     RKNLSLFLSISLLTFWGTCLLGARLYWMGNKPPSFSNSDNPAADSDSLLARTLTFLYL
                     PTKNLWLLLCPDTLSFDWSMDAVPLLKTVCDWRNLHTVAFYSGLLLLAYCGLKNPSLE
                     GECNGKALTNGKQNANGHSCHSDVEYRNSEMKPSFASKVENGIKNCVPQRTQLPSTEN
                     IVILSLSLLIIPFIPATNLFFYVGFVIAERVLYIPSMGFCLLITVGARALYVKVQKRF
                     LKSLVFYATATLIVFYGVKTAIRNGDWQNEEMLYRSGIKVNPAKAWGNLGNVLKSQSK
                     ISEAESAYRNALFYRSNMADMLYNLGLLLQENSRFAEALHYYKLAIGSRPTLASAYLN
                     TGIILMNQGKTEEARRTFLKCSEIPDENLKDPHAHKSSVTSCLYNLGKLYHEQGRYEE
                     ALSVYREAIQKMPRHFAPQSLYNMMGEAYMRLSKLPEAEHWYMESLRSKTDHIPAHLT
                     YGKLLALTGRKSEAEKFFLKAIELDPTKGNCYMHYGQFLLEESRLTEAAEMAKKAAEL
                     DNTEFDVVFNAAHMLRQASLNEAAEKYYDLAARLRPNYPAALMNLGAILHLNGRLQKA
                     EANYLRALQLKPDDVITQSNLRKLWNIMEKQGLKTSKT"
     exon            814..1384
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..2213
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2214..2328
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2329..2414
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2415..2599
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2600..2678
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2679..2800
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2801..2882
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2883..2997
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2998..3061
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3062..5673
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5654..5659
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5673
                     /gene="Tmtc2"
                     /gene_synonym="8430438D04Rik; D330034A10Rik"
                     /note="major polyA site"
ORIGIN      
        1 cgggctagct tagggtggtg ggctggccac gcaggcgcgt ggggccgcga cgagaacccc
       61 acgcgggagc ttttggagtc cccagtcgcc gcttctcact tcactggaga ggggtccggg
      121 agggaaccaa taaagagtga atgtgccgca gccgcggtgg cggccgagtt gctgcgaacg
      181 gccccggagt cgtgcgcccg ggaggcggcg aagggaggcg gttcgcctcg tccctcccac
      241 ccgccccttt ctctctcggt ctctctctct gtctctcctt tcctcccgct ggtcccagat
      301 cctcgctctg tccagccctt tggtcccccc tcttgcactc ttctatctcc gcagtccttt
      361 accttagggc gtcccaaagt gccatgggtt agggtgggga ccgcgagccg cgggaaaagg
      421 acaagccccg attctccgcc ggggggcgtg aagccgatcg ccgcttgcct ctcgcggttg
      481 tcgggtatgg ggagagcggt gtgagcccgc aacccccgga gcacgcagga acagcggagc
      541 ccggcgcccc gaggaggagc ccgggatcgg aagaaggaga ggagggagga cgaggaggcg
      601 ggcggggaag ccggggaaaa gtgcagccaa ggaggagaag gcggcggaag gtggggattg
      661 atgcttctgt tttttgttgc cgctgctgcg ctcgcgctgg gagccaagcc ggagggaagg
      721 cggtggagag atgattgcag agttggtgag cagcgctctg gggctcgcct tgtatctcaa
      781 caccctgagt gctgatttct gctacgatga cagccgtgct atcaagacta atcaagacct
      841 cctgccagaa accccctgga cacacatttt ctacaatgac ttttggggga ctcttctaac
      901 ccacagcggt agccacaagt cctaccggcc actctgcact ctctcttttc gactgaatca
      961 tgccattgga gggctgaatc cctggagcta tcaccttgtc aatgtcctgt tgcatgcggc
     1021 tgtcaccggc ctcttcacac gcttctccaa ggccctcctt ggggacggat actggacctt
     1081 catggctggc ttgatgtttg cttcacaccc catccacacg gaagcggtgg cgggcatcgt
     1141 gggacgagca gatgtgggcg ctagtctctt ttttctcctg tccctgctct gctacattaa
     1201 gcactgttct acaagaggct actctgccag aacttggggc tggttcctgg ggactggatt
     1261 gtgcgctgga tgcagcatgc tttggaaaga gcaaggagtg actgtcctgg cagtttcagc
     1321 cgtttatgac gtctttgtct tccacaggct aaaaatgaag cagatcctgc ctaccattta
     1381 caaaaggaag aacttgtctc tttttctcag cattagcttg ttaacattct gggggacctg
     1441 ccttttgggt gcccggctgt actggatggg caataaacca ccaagcttct ccaactccga
     1501 caaccccgcg gctgactcgg acagcctttt ggctcgcacg ctcaccttct tgtatttacc
     1561 taccaagaac ctctggctat tgctctgccc ggacaccctg agctttgatt ggtccatgga
     1621 tgccgtgcct cttctcaaaa cagtttgtga ttggagaaat ctacacactg tggccttcta
     1681 ttctggactt ctcctccttg cctactgtgg tttgaagaac ccaagtttag aaggagagtg
     1741 caacgggaaa gccttgacaa atggcaagca gaatgcaaat ggccatagct gccactcaga
     1801 cgtggagtac cggaactcag aaatgaagcc cagctttgca tccaaagtag agaatggaat
     1861 taaaaattgt gtaccacaga gaactcagct cccgtccacg gagaacattg ttattctgtc
     1921 cttatctttg ttgataatac cttttattcc tgccacgaac ctgtttttct atgtcggctt
     1981 tgtaatcgca gagcgagtct tatatattcc cagtatgggc ttctgccttc tgatcacagt
     2041 gggtgccaga gccctgtatg tcaaagtaca aaagaggttt ctcaagagct tggtttttta
     2101 tgctactgcc acgttaattg tcttctacgg agtcaagact gcgatcagga acggagactg
     2161 gcagaacgag gagatgctgt atagatcagg gatcaaagtg aacccagcca aagcatgggg
     2221 taaccttgga aatgttctga agagtcagag taaaatctct gaagctgaaa gcgcctacag
     2281 aaatgctctg ttctaccgca gcaacatggc tgacatgctg tataatctag ggttgctgct
     2341 ccaagagaac agcaggtttg cagaagctct tcactattac aaactggcca ttgggagcag
     2401 gcctaccctg gcttctgcct atttaaacac cggcattatt ctcatgaacc aagggaagac
     2461 agaagaagcc cggcggacat tcctcaagtg ttctgagatt ccagatgaga acctgaagga
     2521 tcctcatgca cataagagtt ctgttaccag ctgtctgtac aacctcggga aactctatca
     2581 cgagcaagga cgctatgagg aagccctgag tgtatatagg gaagcaattc agaagatgcc
     2641 aaggcacttt gcccctcaga gcctgtacaa catgatgggt gaagcctaca tgagactgag
     2701 taagcttcct gaagctgagc attggtacat ggaatcactg agatccaaga ccgatcacat
     2761 ccctgctcat ctcacctacg ggaagctgct cgccttaaca ggtcgtaaga gtgaggctga
     2821 aaagttcttc ttgaaggcta ttgagctgga tcctaccaaa ggaaactgtt acatgcacta
     2881 tggtcagttt ctcctagaag agtctcgcct cacagaagca gcagagatgg caaagaaagc
     2941 agctgagctg gacaacacag agttcgatgt cgtcttcaat gctgcccata tgctcagaca
     3001 agcaagcctc aatgaagccg ctgagaagta ttacgatctg gcagcaagac tgagacctaa
     3061 ttaccctgct gctctcatga acctaggggc cattctgcat ctcaatgggc gactgcagaa
     3121 ggctgaggcc aactacctga gggccctgca actcaagcca gatgatgtca tcactcagtc
     3181 taatctccgc aagctgtgga acatcatgga aaagcaaggt ttgaagacct ccaagacctg
     3241 acacaggaga actcccctcc tcctcctccg cttgtcagag ctggcttcat taacagacag
     3301 aacttcccag gagtgctgtg ataaaagctc aagggcgact tcaagcccag gacaaaggtc
     3361 actatggtta ctgccactgc tgggagaatc cactttgcta tgggcacagc ccttaacacc
     3421 aaaaagggga acattgagac cctctgggag ggtgtaaact gttacccgta gactgtaaac
     3481 cagagcacct aagaggattt cggagagttc ctagaaagta gaggggtctc atttgcagat
     3541 gtccactttg gagggagaat ttgaaattct attgtttctt aaacactgtg agaggaagtc
     3601 agagagccca tccagccaca gtgaactctt aagggggtga ttaacacaag tgtaaatcct
     3661 cagtgagcca ccggttgtcc ttatgctgct gccttccctc tttgggacag cctgcttatt
     3721 gattgctgaa gctaatccaa ctttggtttt aacagcgtgt cgggatctgt cagaatctcc
     3781 agttgtttct tggctcgtgc acataaacat tttatggttt gaatgtccta ggcttcctaa
     3841 gtgtttagca tcatttttct gtcatgtctc aaaacgaacg tgtggagaag agctaaccac
     3901 tcgtagaggt gatacaagag gacttagagt ggccttgggt gtgtgtcaca aaggcatttt
     3961 catttcgata ttgtcagtat tgtctcccgt gttgtttagt agatctcatt gtattaattt
     4021 ttttgaaatt atcttctatg atgtctatcc atggttacta tacatgttgt tcactaaggc
     4081 tgaaaaaaaa atggggatat aagtgttcca gttctcataa ctaatcatga ctatatatga
     4141 cacagtcctc ggtgcttcct agccagtggt tgctataata agccaatgca gggtaagtac
     4201 ataaggattg ttgccaacga ctttgtgttt tgaaaagagc tgatatgaaa tacccattgt
     4261 gtcctcgcat acctaaacac cagtgcggat aaatcctgga gggttgtctc attttgctct
     4321 tcctgaaaga tgtcaaaaat aaattatttt aagatgccat ggagtaaaca tcagcctgca
     4381 tatatctcca tcgtctgcca aatgcttttc tcagttaaaa gatattttct catagaaaac
     4441 tgagcgtttt ctttcctcac ctcctattag ctatttcagt gatcatttta taagttaaaa
     4501 gctataaatg aaacaaagaa aaaaattttt taacattttt gccttgtact caaactttac
     4561 tactaaaaat taaattccca gaataccagt ctattagcaa cctctttgta gagaaattaa
     4621 ctattctgta cccaacatat ttatttattt attatccagc catagcaaaa taacaaaaca
     4681 agacaaaaaa gatttgctaa aacagttatt tgctttttct ccttcccttc tatactccat
     4741 gtatatatat atatgtatat ggtcagtgat tccattaaag ggaagctggt catgaaaaaa
     4801 attctatgtt ttctccctat attatatatc atgtcatgta tcgaatacag tggataaaga
     4861 acttgaggta gtgttccaat gacattagtt tctatgagag aacagatgta gtgagagttt
     4921 taatggggtt tatgagaagg ccctctttaa agtgtatatt actctgtaaa catttcactc
     4981 aagggccaga agttaaagta taactaaatc gctgtgttgt cagaataata tttaacatta
     5041 acaagaacaa acccatggtc aaaccaaaaa atagtgggtt gaagtataca tttcattttc
     5101 taatgcattg gcaattcgga tgacaaagaa atttaatata aagctgtaga gataaattca
     5161 gtgtctaacc ttttttttta atttatttaa atagttgtgg cattctagtc tttacatttt
     5221 atgtctgtct tattactgtt ccttctttct aacacttgtt ttctgtaggt tcactgaatg
     5281 cacaatagtc acatttcaca tgactcaact cccaagtggt gttaatcatt gggcctgtgt
     5341 cataaaatgc atggatcttt aatacctaca tgtcgctgac acttttcact acagggctgg
     5401 acttagtaac tgaccaactc ggggggggga gggggcgctg gggtattaga acatgatcaa
     5461 aatgtctctg ctcagggatt tatggtggat cattgcagac agtgctaaaa atgtagagca
     5521 caagacaagt ttactaaatt aaaattttat tttttgaaaa actgttattt gtataaatta
     5581 tcaagatttg taggctttcc ttttgtagaa ataattgttt gatgtgccag agaatttcaa
     5641 ttttgttttg aacaataaag cattgataac aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     5701 a
//
