LOCUS       NM_145403               2227 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus transmembrane protease, serine 4 (Tmprss4), transcript
            variant 1, mRNA.
ACCESSION   NM_145403
VERSION     NM_145403.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Yang,Y.S., Wen,D. and Zhao,X.F.
  TITLE     Transmembrane Protease TMPRSS4 Promotes the Formation and
            Development of Mismatch Repair Deficient Colon Cancer Liver
            Metastasis
  JOURNAL   Bull Exp Biol Med 171 (2), 242-246 (2021)
   PUBMED   34173918
  REMARK    GeneRIF: Transmembrane Protease TMPRSS4 Promotes the Formation and
            Development of Mismatch Repair Deficient Colon Cancer Liver
            Metastasis.
            Erratum:[Bull Exp Biol Med. 2021 Nov;172(1):112. doi:
            10.1007/s10517-021-05343-9.. PMID: 34796428]
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Harbig,A., Mernberger,M., Bittel,L., Pleschka,S., Schughart,K.,
            Steinmetzer,T., Stiewe,T., Nist,A. and Bottcher-Friebertshauser,E.
  TITLE     Transcriptome profiling and protease inhibition experiments
            identify proteases that activate H3N2 influenza A and influenza B
            viruses in murine airways
  JOURNAL   J Biol Chem 295 (33), 11388-11407 (2020)
   PUBMED   32303635
REFERENCE   3  (bases 1 to 2227)
  AUTHORS   Bilinska,K., Jakubowska,P., Von Bartheld,C.S. and Butowt,R.
  TITLE     Expression of the SARS-CoV-2 Entry Proteins, ACE2 and TMPRSS2, in
            Cells of the Olfactory Epithelium: Identification of Cell Types and
            Trends with Age
  JOURNAL   ACS Chem Neurosci 11 (11), 1555-1562 (2020)
   PUBMED   32379417
REFERENCE   4  (bases 1 to 2227)
  AUTHORS   Valero-Jimenez,A., Zuniga,J., Cisneros,J., Becerril,C., Salgado,A.,
            Checa,M., Buendia-Roldan,I., Mendoza-Milla,C., Gaxiola,M., Pardo,A.
            and Selman,M.
  TITLE     Transmembrane protease, serine 4 (TMPRSS4) is upregulated in IPF
            lungs and increases the fibrotic response in bleomycin-induced lung
            injury
  JOURNAL   PLoS One 13 (3), e0192963 (2018)
   PUBMED   29529050
  REMARK    GeneRIF: The lung fibrotic response evaluated at 28 days after
            bleomycin injury was markedly attenuated in the haplodeficient and
            deficient TMPRSS4 mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2227)
  AUTHORS   Kuhn,N., Bergmann,S., Kosterke,N., Lambertz,R.L.O., Keppner,A., van
            den Brand,J.M.A., Pohlmann,S., Weiss,S., Hummler,E., Hatesuer,B.
            and Schughart,K.
  TITLE     The Proteolytic Activation of (H3N2) Influenza A Virus
            Hemagglutinin Is Facilitated by Different Type II Transmembrane
            Serine Proteases
  JOURNAL   J Virol 90 (9), 4298-4307 (2016)
   PUBMED   26889029
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2227)
  AUTHORS   Passero,C.J., Mueller,G.M., Myerburg,M.M., Carattino,M.D.,
            Hughey,R.P. and Kleyman,T.R.
  TITLE     TMPRSS4-dependent activation of the epithelial sodium channel
            requires cleavage of the gamma-subunit distal to the furin cleavage
            site
  JOURNAL   Am J Physiol Renal Physiol 302 (1), F1-F8 (2012)
   PUBMED   21993886
  REMARK    GeneRIF: TMPRSS4 primarily activates ENaC by cleaving basic
            residues within the tract gammaK173-K186 distal to the furin
            cleavage site, thereby releasing a previously defined key
            inhibitory tract encompassing gammaR158-F168 from the
            gamma-subunit.
REFERENCE   7  (bases 1 to 2227)
  AUTHORS   Szabo,R., Hobson,J.P., Christoph,K., Kosa,P., List,K. and
            Bugge,T.H.
  TITLE     Regulation of cell surface protease matriptase by HAI2 is essential
            for placental development, neural tube closure and embryonic
            survival in mice
  JOURNAL   Development 136 (15), 2653-2663 (2009)
   PUBMED   19592578
REFERENCE   8  (bases 1 to 2227)
  AUTHORS   Andreasen,D., Vuagniaux,G., Fowler-Jaeger,N., Hummler,E. and
            Rossier,B.C.
  TITLE     Activation of epithelial sodium channels by mouse channel
            activating proteases (mCAP) expressed in Xenopus oocytes requires
            catalytic activity of mCAP3 and mCAP2 but not mCAP1
  JOURNAL   J Am Soc Nephrol 17 (4), 968-976 (2006)
   PUBMED   16524950
  REMARK    GeneRIF: mutations in conserved residues of mCAP2 located in two
            protein-protein interacting domains significantly modulated ENaC
            activation
REFERENCE   9  (bases 1 to 2227)
  AUTHORS   Kim,T.S., Heinlein,C., Hackman,R.C. and Nelson,P.S.
  TITLE     Phenotypic analysis of mice lacking the Tmprss2-encoded protease
  JOURNAL   Mol Cell Biol 26 (3), 965-975 (2006)
   PUBMED   16428450
REFERENCE   10 (bases 1 to 2227)
  AUTHORS   Vuagniaux,G., Vallet,V., Jaeger,N.F., Hummler,E. and Rossier,B.C.
  TITLE     Synergistic activation of ENaC by three membrane-bound
            channel-activating serine proteases (mCAP1, mCAP2, and mCAP3) and
            serum- and glucocorticoid-regulated kinase (Sgk1) in Xenopus
            Oocytes
  JOURNAL   J Gen Physiol 120 (2), 191-201 (2002)
   PUBMED   12149280
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166051.5 and AC122305.4.
            
            On Jun 21, 2018 this sequence version replaced NM_145403.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY043240.1, BC021368.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-251               AC166051.5         27204-27454         c
            252-285             AC166051.5         13548-13581         c
            286-399             AC166051.5         9234-9347           c
            400-552             AC166051.5         7660-7812           c
            553-682             AC166051.5         6597-6726           c
            683-784             AC122305.4         190697-190798       c
            785-825             AC122305.4         189450-189490       c
            826-985             AC122305.4         187753-187912       c
            986-1152            AC122305.4         186433-186599       c
            1153-1251           AC122305.4         185506-185604       c
            1252-1394           AC122305.4         185149-185291       c
            1395-1544           AC122305.4         183905-184054       c
            1545-2227           AC122305.4         182773-183455       c
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 24.84 cM"
     gene            1..2227
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /note="transmembrane protease, serine 4"
                     /db_xref="GeneID:214523"
                     /db_xref="MGI:MGI:2384877"
     exon            1..251
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             249..1556
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     channel-activating protease 2; membrane-type serine
                     protease 2; channel-activating protease 4"
                     /codon_start=1
                     /product="transmembrane protease serine 4 isoform 1"
                     /protein_id="NP_663378.1"
                     /db_xref="CCDS:CCDS40607.1"
                     /db_xref="GeneID:214523"
                     /db_xref="MGI:MGI:2384877"
                     /translation="MESDSGQPLNNRDIVPFRKPRRPQETFKKVGIPIIAVLLSLIAL
                     VIVALLIKVILDKYYFICGSPLTFIQRGQLCDGHLDCASGEDEEHCVKDFPEKPGVAV
                     RLSKDRSTLQVLDAATGTWASVCFDNFTEALAKTACRQMGYDSQPAFRAVEIRPDQNL
                     PVAQVTGNSQELQVQNGSRSCLSGSLVSLRCLDCGKSLKTPRVVGGVEAPVDSWPWQV
                     SIQYNKQHVCGGSILDPHWILTAAHCFRKYLDVSSWKVRAGSNILGNSPSLPVAKIFI
                     AEPNPLYPKEKDIALVKLQMPLTFSGSVRPICLPFSDEVLVPATPVWVIGWGFTEENG
                     GKMSDMLLQASVQVIDSTRCNAEDAYEGEVTAEMLCAGTPQGGKDTCQGDSGGPLMYH
                     SDKWQVVGIVSWGHGCGGPSTPGVYTKVTAYLNWIYNVRKSEM"
     exon            252..285
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            286..399
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            400..552
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            553..682
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            683..784
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            785..825
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            826..985
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1152
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1251
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1394
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1544
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..2227
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2203..2208
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /note="hexamer: AATAAA"
     polyA_site      2227
                     /gene="Tmprss4"
                     /gene_synonym="CAP2; mCAP2"
                     /note="major polyA site"
ORIGIN      
        1 aagtcactcc tagatacagt ggcaggtggt agctggcttg cggaaggaag aggaagaaga
       61 gaatgtgggc catcaaggag caaggccagc cttgcacttg ggccccctct gctcagtgct
      121 gaccagggct ttctgagccg cttcctaatg aggctcattt gaagaccccc ccccaccccc
      181 ctcctgctgt cttgggtggc agagctagct ccaggctgta agaaaattag gaggattacc
      241 aaagcagtat ggagtcagac agtggccaac ccctcaacaa ccgtgatatt gttccctttc
      301 gcaaaccccg aaggccccag gagaccttca aaaaggtggg gatccccatc attgcagtgc
      361 tgctgagcct gatagccctc gtgattgtgg cccttctcat caaggtgatt ctggataaat
      421 actacttcat ctgcggcagt cccctgacct tcattcagag gggccagttg tgtgacggcc
      481 accttgactg cgcctcaggg gaggatgagg aacactgtgt caaggacttc cctgaaaagc
      541 ccggagtggc agtccggctc tccaaggaca gatccaccct gcaggtgctg gatgcagcca
      601 cagggacctg ggcctcagtc tgtttcgaca acttcacaga agcactggcc aagacagcct
      661 gcagacagat gggctatgac agccagcccg ctttcagagc agtggagatc cgtccagatc
      721 agaacctccc tgttgctcaa gtcacaggaa acagccagga acttcaggtg cagaatggaa
      781 gcagatcctg cctctcaggc tccctggttt ccttgcgctg ccttgactgt ggaaagagcc
      841 tgaagactcc tcgtgtggtg ggtggggtgg aggcccctgt ggattcttgg ccgtggcagg
      901 tcagcatcca gtacaacaag cagcatgtct gtggtgggag catcctggat ccccactgga
      961 tcctcacagc agcccactgc ttcaggaagt atcttgatgt gtcaagctgg aaggtcaggg
     1021 caggctcaaa catactgggt aactctccat ccttgcctgt ggccaagatc ttcatcgctg
     1081 aacccaatcc tctgtacccc aaagagaagg acattgccct tgttaagctg cagatgccac
     1141 tcacattctc aggctcagtc aggcccatct gcctgccctt ctctgatgag gtgcttgtcc
     1201 cagccacacc agtctgggtc attggatggg gctttacaga agaaaacgga ggaaagatgt
     1261 ctgacatgct actgcaggca tcagtccagg tcattgacag cacacggtgc aatgcagagg
     1321 atgcctacga aggggaagtg accgctgaga tgctgtgtgc aggtacccca cagggtggca
     1381 aggacacctg ccagggtgac agtggtgggc ctttgatgta ccattctgac aagtggcagg
     1441 tagtaggcat cgtgagctgg ggccatggat gcggcggccc aagtactcct ggagtgtata
     1501 ccaaggtcac tgcctatctc aactggatct acaatgttcg gaagtctgag atgtaacgct
     1561 gccgtccccc acatccagaa gctgcttccc ttcagaccta cctacggcat gacccctcaa
     1621 agtcagatat gggacaagag cctccttgaa caaactctgg tatccctgca gcaagcaagg
     1681 atacattgca gaggtgcccg gagtggagtc agatgggcta gctcagccac ccctgcatct
     1741 cccaaaccct gggagacatg tggcccatgg gagtaaatcc aggacattga ctcaactctc
     1801 agaagtgtta ttcagtcaag gaggctctcc cttccactga aggaaggaaa gtcagctctc
     1861 tcctgaaagg ccagatcact ggctgagtag atgagacaag ggtatgaaag gcctttgcca
     1921 tcttctttgc ccagtcctga aagcactgac gtaagagacc agtcagttct aatgtaaggt
     1981 gtatatttta gtgtcagggt attgcaattg tcacctctgt ggtcaatatc attaaacagg
     2041 tatgagaatt cgctggcata gacttcctgg tctgcttaat aagaatccaa ctaaggatgt
     2101 cacatgacag tttcccagaa aatgtgaaca agtgtccatc tgacacacgg caccaatgac
     2161 aaaccaaaga agttattctg cctgagtctc agttgctgaa ctaataaatt agctgcggtt
     2221 tcttgca
//
