LOCUS       NM_027025                957 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus transmembrane and immunoglobulin domain containing 3
            (Tmigd3), transcript variant 2, mRNA.
ACCESSION   NM_027025
VERSION     NM_027025.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Shide,K., Kameda,T., Kamiunten,A., Ozono,Y., Tahira,Y.,
            Yokomizo-Nakano,T., Kubota,S., Ono,M., Ikeda,K., Sekine,M.,
            Akizuki,K., Nakamura,K., Hidaka,T., Kubuki,Y., Iwakiri,H.,
            Hasuike,S., Nagata,K., Sashida,G. and Shimoda,K.
  TITLE     Calreticulin haploinsufficiency augments stem cell activity and is
            required for onset of myeloproliferative neoplasms in mice
  JOURNAL   Blood 136 (1), 106-118 (2020)
   PUBMED   32219445
REFERENCE   2  (bases 1 to 957)
  AUTHORS   Shide,K., Kameda,T., Yamaji,T., Sekine,M., Inada,N., Kamiunten,A.,
            Akizuki,K., Nakamura,K., Hidaka,T., Kubuki,Y., Shimoda,H.,
            Kitanaka,A., Honda,A., Sawaguchi,A., Abe,H., Miike,T., Iwakiri,H.,
            Tahara,Y., Sueta,M., Hasuike,S., Yamamoto,S., Nagata,K. and
            Shimoda,K.
  TITLE     Calreticulin mutant mice develop essential thrombocythemia that is
            ameliorated by the JAK inhibitor ruxolitinib
  JOURNAL   Leukemia 31 (5), 1136-1144 (2017)
   PUBMED   27807369
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121847.2, AK005581.1 and BC038878.1.
            
            On Sep 24, 2013 this sequence version replaced NM_027025.3.
            
            Summary: This gene encodes a transmembrane and immunoglobulin
            domain-containing protein. Alternative splicing results in multiple
            transcript variants, one of which shares its 3' terminal exon with
            that of the overlapping adenosine A3 receptor gene (GeneID:11542).
            [provided by RefSeq, Nov 2014].
            
            Transcript Variant: This variant (2) encodes the longer isoform
            (2), which is a transmembrane and immunoglobulin domain-containing
            protein and it does not share any similarity with the adenosine A3
            receptor.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.70219.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                AC121847.2         62013-62061         c
            50-440              AK005581.1         2-392
            441-834             BC038878.1         321-714
            835-955             BC038878.1         718-838
            956-957             AC121847.2         8875-8876           c
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3"
     gene            1..957
                     /gene="Tmigd3"
                     /gene_synonym="1700001d09rik; 4930578J19Rik;
                     Tg(H2-K-CALR*)del52Shmd"
                     /note="transmembrane and immunoglobulin domain containing
                     3"
                     /db_xref="GeneID:69296"
                     /db_xref="MGI:MGI:5604098"
     exon            1..154
                     /gene="Tmigd3"
                     /gene_synonym="1700001d09rik; 4930578J19Rik;
                     Tg(H2-K-CALR*)del52Shmd"
                     /inference="alignment:Splign:2.1.0"
     exon            155..256
                     /gene="Tmigd3"
                     /gene_synonym="1700001d09rik; 4930578J19Rik;
                     Tg(H2-K-CALR*)del52Shmd"
                     /inference="alignment:Splign:2.1.0"
     CDS             217..846
                     /gene="Tmigd3"
                     /gene_synonym="1700001d09rik; 4930578J19Rik;
                     Tg(H2-K-CALR*)del52Shmd"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; transmembrane domain-containing protein TMIGD3"
                     /codon_start=1
                     /product="transmembrane domain-containing protein TMIGD3
                     isoform 2 precursor"
                     /protein_id="NP_081301.2"
                     /db_xref="CCDS:CCDS38582.1"
                     /db_xref="GeneID:69296"
                     /db_xref="MGI:MGI:5604098"
                     /translation="MEFLLLLSLALFSDAMVMDEKVKSGVELETASAVCVYDAYYKDH
                     TKYWCRGYFRDSCNIIAFTPNSTNRVALKDTGNQLIITISCLVKEDTGWYWCGIQRDL
                     ARDDMDFTQLIVTDNREDRANGFSSDPSGNRTRSCRASKAVQKAEGSRMSILIICILI
                     TSLGIIFIISHLSRGRRSQRNREVTGKSISRNPQASQGPSMVSITLARI"
     exon            257..598
                     /gene="Tmigd3"
                     /gene_synonym="1700001d09rik; 4930578J19Rik;
                     Tg(H2-K-CALR*)del52Shmd"
                     /inference="alignment:Splign:2.1.0"
     exon            599..665
                     /gene="Tmigd3"
                     /gene_synonym="1700001d09rik; 4930578J19Rik;
                     Tg(H2-K-CALR*)del52Shmd"
                     /inference="alignment:Splign:2.1.0"
     exon            666..766
                     /gene="Tmigd3"
                     /gene_synonym="1700001d09rik; 4930578J19Rik;
                     Tg(H2-K-CALR*)del52Shmd"
                     /inference="alignment:Splign:2.1.0"
     exon            767..834
                     /gene="Tmigd3"
                     /gene_synonym="1700001d09rik; 4930578J19Rik;
                     Tg(H2-K-CALR*)del52Shmd"
                     /inference="alignment:Splign:2.1.0"
     exon            835..957
                     /gene="Tmigd3"
                     /gene_synonym="1700001d09rik; 4930578J19Rik;
                     Tg(H2-K-CALR*)del52Shmd"
                     /inference="alignment:Splign:2.1.0"
     regulatory      928..933
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmigd3"
                     /gene_synonym="1700001d09rik; 4930578J19Rik;
                     Tg(H2-K-CALR*)del52Shmd"
                     /note="hexamer: AATAAA"
     polyA_site      957
                     /gene="Tmigd3"
                     /gene_synonym="1700001d09rik; 4930578J19Rik;
                     Tg(H2-K-CALR*)del52Shmd"
                     /note="major polyA site"
ORIGIN      
        1 gcatcaccgg aggatgcctg ggtgtcagct tgccttcagg tgctcagcta gacttcatca
       61 agcacatgtg gagagctctg tattaggtta atgtgctaac attaacactg gtgagcaaag
      121 tatgatcctc gtgctcaaaa cgctgagaat ccagatccag ggttcctgag atcctcgctc
      181 taatcattcc tgccgaaggc tcacttcctt ctcagcatgg agttcctcct ccttctctcc
      241 ttggctctct tttccgatgc catggtcatg gatgaaaagg tgaaatcagg tgttgagctg
      301 gagacagctt ctgcagtctg tgtctatgat gcctactaca aggaccacac caagtactgg
      361 tgccgtggct acttcaggga ctcgtgcaac attattgcct tcacccctaa tagcaccaac
      421 cgtgtggccc tgaaggatac aggaaaccaa ctcattatca ctatatcctg cctggtcaag
      481 gaggacacag gctggtactg gtgtggtatc cagcgggact tggccagaga tgacatggat
      541 tttacacagc tgattgtgac tgacaataga gaagaccggg ccaatggctt ttcatctgac
      601 ccttcaggca ataggacccg gagctgtagg gcttccaaag ctgtccaaaa ggcagagggc
      661 tcccgaatgt ccattctgat catttgtata ctgattacca gtttggggat catctttata
      721 atcagtcact tgtccagagg aaggcgaagc caaagaaata gagaagttac tggcaaaagc
      781 atcagtagaa acccacaagc aagccaaggc ccgtctatgg tctccataac tttggcaagg
      841 atttgaagtt ctttccacgt gtcctgactc aagggaatgg cttgaactga acatgtgtga
      901 ctgaagattt ctttcactta gctcctaaat aaagtgatgt cattacagta gaatata
//
