LOCUS       NM_146260               2786 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus transmembrane inner ear (Tmie), mRNA.
ACCESSION   NM_146260
VERSION     NM_146260.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Wang,P., Miller,K.K., He,E., Dhawan,S.S., Cunningham,C.L. and
            Grillet,N.
  TITLE     LOXHD1 is indispensable for maintaining TMC1 auditory
            mechanosensitive channels at the site of force transmission
  JOURNAL   Nat Commun 15 (1), 7865 (2024)
   PUBMED   39256406
  REMARK    Erratum:[Nat Commun. 2024 Oct 15;15(1):8878. doi:
            10.1038/s41467-024-52972-5.. PMID: 39406713]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Jiang,Q., Zou,W., Li,S., Qiu,X., Zhu,L., Kang,L. and Muller,U.
  TITLE     Sequence variations and accessory proteins adapt TMC functions to
            distinct sensory modalities
  JOURNAL   Neuron 112 (17), 2922-2937 (2024)
   PUBMED   38986620
REFERENCE   3  (bases 1 to 2786)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2786)
  AUTHORS   Krey,J.F., Chatterjee,P., Halford,J., Cunningham,C.L., Perrin,B.J.
            and Barr-Gillespie,P.G.
  TITLE     Control of stereocilia length during development of hair bundles
  JOURNAL   PLoS Biol 21 (4), e3001964 (2023)
   PUBMED   37011103
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2786)
  AUTHORS   Ji,Y.R., Tona,Y., Wafa,T., Christman,M.E., Tourney,E.D., Jiang,T.,
            Ohta,S., Cheng,H., Fitzgerald,T., Fritzsch,B., Jones,S.M.,
            Cullen,K.E. and Wu,D.K.
  TITLE     Function of bidirectional sensitivity in the otolith organs
            established by transcription factor Emx2
  JOURNAL   Nat Commun 13 (1), 6330 (2022)
   PUBMED   36280667
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2786)
  AUTHORS   Cho,K.I., Suh,J.G., Lee,J.W., Hong,S.H., Kang,T.C., Oh,Y.S. and
            Ryoo,Z.Y.
  TITLE     The circling mouse (C57BL/6J-cir) has a 40-kilobase genomic
            deletion that includes the transmembrane inner ear (tmie) gene
  JOURNAL   Comp Med 56 (6), 476-481 (2006)
   PUBMED   17219777
  REMARK    GeneRIF: characterized the circling mutation as a 40-kilobase
            deletion that includes the transmembrane inner ear (tmie) gene
REFERENCE   7  (bases 1 to 2786)
  AUTHORS   Jones,S.M., Johnson,K.R., Yu,H., Erway,L.C., Alagramam,K.N.,
            Pollak,N. and Jones,T.A.
  TITLE     A quantitative survey of gravity receptor function in mutant mouse
            strains
  JOURNAL   J Assoc Res Otolaryngol 6 (4), 297-310 (2005)
   PUBMED   16235133
REFERENCE   8  (bases 1 to 2786)
  AUTHORS   Mitchem,K.L., Hibbard,E., Beyer,L.A., Bosom,K., Dootz,G.A.,
            Dolan,D.F., Johnson,K.R., Raphael,Y. and Kohrman,D.C.
  TITLE     Mutation of the novel gene Tmie results in sensory cell defects in
            the inner ear of spinner, a mouse model of human hearing loss DFNB6
  JOURNAL   Hum Mol Genet 11 (16), 1887-1898 (2002)
   PUBMED   12140191
  REMARK    GeneRIF: Mutation of the novel gene Tmie results in sensory cell
            defects in the inner ear of spinner, a mouse model of human hearing
            loss DFNB6.
REFERENCE   9  (bases 1 to 2786)
  AUTHORS   Vargas,G.A., Fantino,E., George-Nascimento,C., Gargus,J.J. and
            Haigler,H.T.
  TITLE     Reduced epidermal growth factor receptor expression in hypohidrotic
            ectodermal dysplasia and Tabby mice
  JOURNAL   J Clin Invest 97 (11), 2426-2432 (1996)
   PUBMED   8647934
REFERENCE   10 (bases 1 to 2786)
  AUTHORS   DEOL,M.S. and ROBINS,M.W.
  TITLE     The spinner mouse
  JOURNAL   J Hered 53, 133-136 (1962)
   PUBMED   14027064
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC139378.4.
            
            On Jun 19, 2018 this sequence version replaced NM_146260.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK030341.1, AF481143.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-804               AC139378.4         195208-196011       c
            805-1016            AC139378.4         191566-191777       c
            1017-1247           AC139378.4         189734-189964       c
            1248-1365           AC139378.4         186569-186686       c
            1366-1515           AC139378.4         183386-183535       c
            1516-2786           AC139378.4         181625-182895       c
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 60.79 cM"
     gene            1..2786
                     /gene="Tmie"
                     /gene_synonym="5131400L21Rik; Mm.87012; sr"
                     /note="transmembrane inner ear"
                     /db_xref="GeneID:20776"
                     /db_xref="MGI:MGI:2159400"
     exon            1..804
                     /gene="Tmie"
                     /gene_synonym="5131400L21Rik; Mm.87012; sr"
                     /inference="alignment:Splign:2.1.0"
     exon            805..1016
                     /gene="Tmie"
                     /gene_synonym="5131400L21Rik; Mm.87012; sr"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1247
                     /gene="Tmie"
                     /gene_synonym="5131400L21Rik; Mm.87012; sr"
                     /inference="alignment:Splign:2.1.0"
     CDS             1152..1613
                     /gene="Tmie"
                     /gene_synonym="5131400L21Rik; Mm.87012; sr"
                     /note="transmembrane inner ear expressed protein; spinner"
                     /codon_start=1
                     /product="transmembrane inner ear expressed protein
                     precursor"
                     /protein_id="NP_666372.1"
                     /db_xref="CCDS:CCDS23578.1"
                     /db_xref="GeneID:20776"
                     /db_xref="MGI:MGI:2159400"
                     /translation="MAGRQHGSGRLWALGGAALGACLAGVATQLVEPSTAPPKPKPPP
                     LTKETVVFWDMRLWHVVGIFSLFVLSIIITLCCVFNCRVPRTRKEIEARYLQRKAAKM
                     YTDKLETVPPLNELTEIPGEDKKKKKKDSVDTVAIKVEEDEKNEAKKKGEK"
     exon            1248..1365
                     /gene="Tmie"
                     /gene_synonym="5131400L21Rik; Mm.87012; sr"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1515
                     /gene="Tmie"
                     /gene_synonym="5131400L21Rik; Mm.87012; sr"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..2786
                     /gene="Tmie"
                     /gene_synonym="5131400L21Rik; Mm.87012; sr"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2746..2751
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmie"
                     /gene_synonym="5131400L21Rik; Mm.87012; sr"
                     /note="hexamer: AATAAA"
     polyA_site      2766
                     /gene="Tmie"
                     /gene_synonym="5131400L21Rik; Mm.87012; sr"
                     /note="major polyA site"
ORIGIN      
        1 agagcctctc ccgccagact gcgaaagctc cccttcggtc ggtccgcgag ctccccagga
       61 tcgcaccctg gctgtctttc gccttttacc tccctctcca gatccgagga cggcaggcag
      121 tgcctggggc caccccgatt tccttaccca ccaactctgc ccaggctgta gctctgaaat
      181 cttgccgacc ccctttgggt cactgggcac cccccccccc tcaccctcac acacccgcga
      241 aggagccctg ggtagattcg ggaaacttgt tgcttagagc agttggccat gatgatccga
      301 agaccctgaa ctgggcatgc tttggtagca gctactgctg tgtgtaacct tgaaggaggc
      361 cccctgcatt cattccgggt acagcctgaa gctggccagt ttcctgtgcc aaagccttcg
      421 accttggaaa gagcagcgca gttccaaacc aaagtcttgg aaggaggagg gaccatggtt
      481 caaacctgta acaaagaggc ctgctgtcca gctgccgggc tcccttttgg ccagcaagca
      541 agtcacccct ccacctgcag tggcacccgg cctctaggag ctgctgacta gcctggctga
      601 gttgcttcta agccttccaa gcctgagtac agagagaagc ctggggagga gacagctgtg
      661 aaagagcaag gatagtccac ctgcccccta cccaagtttt ggacagcctc catgcattct
      721 gccagagaga aaagagactt ctaagagttt ctcagaaggg gcctgccaac ttcagcatcc
      781 ctggctccct tggggtgctt tctggagaaa atgtcaaatc cttctgggac tggagttact
      841 gatagttgag agccaccccg aggatgctgg aaatcaaact tgggacctct gcaagagcaa
      901 caaatgctcc taaccactga gcctcttcca agagaatgaa ctttgaacat cagtaccttc
      961 ctgggctgag cagcaaggga agtcctgtga gcagcagacc aatgtgtcct gctaagccag
     1021 aactctccgc ggccgctgcc accaccaccg tctccgcctg ctggtggcca gggacagtca
     1081 cctggtagct ctgtgcatca gccccctgga ctctcaggac ctgcaccgga gcgcgcggca
     1141 aacgagagaa catggccggg aggcagcatg gctcggggcg gctctgggcg ctgggcggcg
     1201 ccgcgctggg ggcttgcctg gcgggtgtcg ccacgcagct ggtagagccc agcaccgcac
     1261 cgccaaagcc caagccgccc ccactgacca aggagactgt ggtgttctgg gacatgcgcc
     1321 tgtggcacgt ggtgggcatc ttttcgctct tcgtgttgtc catcatcatc acgctatgct
     1381 gtgtcttcaa ctgccgggtg ccacggacgc ggaaggagat cgaggctcgg tatctacagc
     1441 gaaaggcggc caagatgtac acagacaagc tggagactgt gcctcccctt aatgaactca
     1501 cagaaatccc tggagaggac aagaagaaaa agaagaagga cagtgtggac acagtggcta
     1561 tcaaggtaga ggaggacgag aagaatgagg ccaagaagaa aggagagaaa tgagggcggc
     1621 ttcctgaggt ggcagccggg gctggcaggc cctgaagcac aagttcaggc cggatccggg
     1681 catcaggact tcgtgttcat acatggactg cagaggcggt ggtccaaggg gaagccaagg
     1741 ccatggtccg ttacctcatg gcccaaaaga tgtagggaca agacttcact agtgtccatc
     1801 ctgggctctg ttcctgtcac atcatccatc tggcacttct cattggccac tgtccttcca
     1861 tagttataga gtggcctcaa actcctctcc ccgtccagac aagcttccat cccacctttc
     1921 ttgctagggg cagaagtggc tgtgaaggag tcagggactg gtcgtttagg atggcaagac
     1981 tgaacaggtt gtccaaaaac agggagggag ggctggaggg gggcttgtga tgtgagagat
     2041 tctctgacat agctagatag ccgcctcaaa tagcaaggag attgccccaa gttggctgct
     2101 agggtggccc cattagcagg agagtccctg acctggcaga tacaaagctt tgaaaacaag
     2161 gtaacagtgt gacagacctg aggccactga ggggctggca ggaggcccag ggcctggctc
     2221 aggggtgggg tctaaaacac agaggactga agtgagtgag cctggacctc ctcatgggaa
     2281 tcaagaaata cttaagtcgc agggcaccag cgcggactac tacagttgcc ttttctatgg
     2341 ccaggactct ctgatgatgg cagcagtcag ccatctgcct gctgaacata gtggtgtgtg
     2401 ggtccacttc tgccatgtcc cttactaaaa caaaaaaaac aaaaaaaaca aaaaaaaaat
     2461 ctctgccaag aagaaaattg aaacgctggc tctagagagg agagatctac aaggcgcttc
     2521 cagcatcaaa atgaggagct ctgaccagag ctggggactg agaccctggg aagactttcc
     2581 aggtggaaag cctgctaaaa aggtatccgg aggaaatttg taaataaagc tgggtgcctt
     2641 ctcaggccta gacatagaaa acttcatcct gagatggaat tccaccccgg ccccatgctt
     2701 tgtcaggccc ttggggactc ctgtagaagt gacacaacaa tacataataa aaggcgcctc
     2761 agacaagcct gacgttctct ccctga
//
