LOCUS       NM_001195710            1198 bp    mRNA    linear   ROD 08-JUN-2024
DEFINITION  Mus musculus transmembrane protein 95 (Tmem95), mRNA.
ACCESSION   NM_001195710 XM_484042 XM_978002
VERSION     NM_001195710.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Inoue,N. and Wada,I.
  TITLE     Deletion of the initial methionine codon of the Tmem95 gene causes
            subfertility, but not complete infertility, in male mice
  JOURNAL   Biol Reprod 106 (3), 378-381 (2022)
   PUBMED   34982145
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Matsumura,T., Noda,T., Satouh,Y., Morohoshi,A., Yuri,S., Ogawa,M.,
            Lu,Y., Isotani,A. and Ikawa,M.
  TITLE     Sperm IZUMO1 Is Required for Binding Preceding Fusion With Oolemma
            in Mice and Rats
  JOURNAL   Front Cell Dev Biol 9, 810118 (2022)
   PUBMED   35096839
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1198)
  AUTHORS   Lamas-Toranzo,I., Hamze,J.G., Bianchi,E., Fernandez-Fuertes,B.,
            Perez-Cerezales,S., Laguna-Barraza,R., Fernandez-Gonzalez,R.,
            Lonergan,P., Gutierrez-Adan,A., Wright,G.J., Jimenez-Movilla,M. and
            Bermejo-Alvarez,P.
  TITLE     TMEM95 is a sperm membrane protein essential for mammalian
            fertilization
  JOURNAL   Elife 9, e53913 (2020)
   PUBMED   32484434
  REMARK    GeneRIF: TMEM95 is a sperm membrane protein essential for mammalian
            fertilization.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1198)
  AUTHORS   Noda,T., Lu,Y., Fujihara,Y., Oura,S., Koyano,T., Kobayashi,S.,
            Matzuk,M.M. and Ikawa,M.
  TITLE     Sperm proteins SOF1, TMEM95, and SPACA6 are required for
            sperm-oocyte fusion in mice
  JOURNAL   Proc Natl Acad Sci U S A 117 (21), 11493-11502 (2020)
   PUBMED   32393636
  REMARK    GeneRIF: Sperm proteins SOF1, TMEM95, and SPACA6 are required for
            sperm-oocyte fusion in mice.
REFERENCE   5  (bases 1 to 1198)
  AUTHORS   Pausch,H., Kolle,S., Wurmser,C., Schwarzenbacher,H., Emmerling,R.,
            Jansen,S., Trottmann,M., Fuerst,C., Gotz,K.U. and Fries,R.
  TITLE     A nonsense mutation in TMEM95 encoding a nondescript transmembrane
            protein causes idiopathic male subfertility in cattle
  JOURNAL   PLoS Genet 10 (1), e1004044 (2014)
   PUBMED   24391514
REFERENCE   6  (bases 1 to 1198)
  AUTHORS   Church,D.M., Goodstadt,L., Hillier,L.W., Zody,M.C., Goldstein,S.,
            She,X., Bult,C.J., Agarwala,R., Cherry,J.L., DiCuccio,M.,
            Hlavina,W., Kapustin,Y., Meric,P., Maglott,D., Birtle,Z.,
            Marques,A.C., Graves,T., Zhou,S., Teague,B., Potamousis,K.,
            Churas,C., Place,M., Herschleb,J., Runnheim,R., Forrest,D.,
            Amos-Landgraf,J., Schwartz,D.C., Cheng,Z., Lindblad-Toh,K.,
            Eichler,E.E. and Ponting,C.P.
  CONSRTM   Mouse Genome Sequencing Consortium
  TITLE     Lineage-specific biology revealed by a finished genome assembly of
            the mouse
  JOURNAL   PLoS Biol 7 (5), e1000112 (2009)
   PUBMED   19468303
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845465.2.
            
            On Feb 15, 2019 this sequence version replaced NM_001195710.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849384
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-209               AL845465.2         24894-25102         c
            210-266             AL845465.2         24587-24643         c
            267-347             AL845465.2         24346-24426         c
            348-368             AL845465.2         24228-24248         c
            369-449             AL845465.2         24008-24088         c
            450-537             AL845465.2         23834-23921         c
            538-1198            AL845465.2         23101-23761         c
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 42.91 cM"
     gene            1..1198
                     /gene="Tmem95"
                     /gene_synonym="432576"
                     /note="transmembrane protein 95"
                     /db_xref="GeneID:100503361"
                     /db_xref="MGI:MGI:3779488"
     exon            1..209
                     /gene="Tmem95"
                     /gene_synonym="432576"
                     /inference="alignment:Splign:2.1.0"
     CDS             38..568
                     /gene="Tmem95"
                     /gene_synonym="432576"
                     /note="sperm-egg fusion protein TMEM95"
                     /codon_start=1
                     /product="sperm-egg fusion protein TMEM95 precursor"
                     /protein_id="NP_001182639.1"
                     /db_xref="CCDS:CCDS56783.1"
                     /db_xref="GeneID:100503361"
                     /db_xref="MGI:MGI:3779488"
                     /translation="MWVLALGGAFLAVAKACIFCRLQDHALANRLAQLNNQTKPKWKW
                     KEWASPDFSAFALDEVSMKQVTEKTHRVLRVIEKKGSTSLIPLYWQWLQKTRIPQYTR
                     EALCAPVCRGSTILYNCSTCEGKEESCWPQKHCYPDSHDLWDARILLLCIFGIVLLSG
                     VVSLQVEYLNLQAKDL"
     exon            210..266
                     /gene="Tmem95"
                     /gene_synonym="432576"
                     /inference="alignment:Splign:2.1.0"
     exon            267..347
                     /gene="Tmem95"
                     /gene_synonym="432576"
                     /inference="alignment:Splign:2.1.0"
     exon            348..368
                     /gene="Tmem95"
                     /gene_synonym="432576"
                     /inference="alignment:Splign:2.1.0"
     exon            369..449
                     /gene="Tmem95"
                     /gene_synonym="432576"
                     /inference="alignment:Splign:2.1.0"
     exon            450..537
                     /gene="Tmem95"
                     /gene_synonym="432576"
                     /inference="alignment:Splign:2.1.0"
     exon            538..1198
                     /gene="Tmem95"
                     /gene_synonym="432576"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1175..1180
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem95"
                     /gene_synonym="432576"
                     /note="hexamer: AATAGA"
     polyA_site      1198
                     /gene="Tmem95"
                     /gene_synonym="432576"
                     /note="major polyA site"
ORIGIN      
        1 gagggggtcc aactccttcc ataccccagc aggcttcatg tgggtgctgg ctctgggtgg
       61 ggctttcttg gccgtggcca aggcttgtat cttctgtcgt ctccaagatc atgccttggc
      121 aaaccgcctg gctcagctca ataaccagac gaagccgaag tggaaatgga aggaatgggc
      181 ttccccagat ttctcggcct ttgccttaga tgaggtctca atgaagcaag tcacagagaa
      241 aactcaccga gtcctgagag tcatagagaa aaaaggatcc acctccttga ttccattata
      301 ctggcaatgg cttcagaaga ccaggatccc ccagtacacc agggaagctc tttgtgctcc
      361 tgtctgccgg ggcagcacca tcctgtacaa ctgctccacc tgcgagggca aggaggagtc
      421 ttgttggccc caaaagcact gctacccaga cagtcacgat ctgtgggacg ctaggattct
      481 gctcctatgt atcttcggaa ttgtcctgct ttcgggtgtt gtgagcctcc aagtggagta
      541 cctgaacctg caagcaaaag acttgtgaag aggaggacag cttttccagc cccagctcta
      601 agaaatctgt gcccacaatt tccacatccc ttagggtggg aacacttttt agacccagcc
      661 ccaaagcctc agtctttcag accctaaacc tcagacttct gctatcaata ttcgtgtcag
      721 atggcctcca ggaacccaag cacccacagc tggaaagtcc ctccctttct gtgttccacc
      781 aatcaaacag agcagcagat gatgccagga aattatctac agaaggaaag aatcgcttac
      841 caccaccact cccacatccc ctctgtccat tcccggaaag cgggggactg ttggcctgta
      901 gatgttgctc taggccctcc aatgactgat ccggaatccg ggaatgaggg ttctggaaaa
      961 ctctctccct ctggggtgag accacatcag tcaacccgct ggcatacagt ccctccattg
     1021 tattaatatt gtgcagcctg gacctccccc tccttctggg cctgtgtaaa tctgctgtgt
     1081 gatgctttcc attcctgtct gactccatct gcaacttggg caggcagatg ggatgggaat
     1141 tcttaggaat tcttgttaga ggtgcccccc ccccaataga tatttagcaa tgtctgga
//
