LOCUS       NM_001368840            5298 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus transmembrane protein 8B (Tmem8b), transcript variant
            3, mRNA.
ACCESSION   NM_001368840 XM_011250027
VERSION     NM_001368840.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL732626.8.
            
            On Mar 4, 2019 this sequence version replaced XM_011250027.2.
            
            Transcript Variant: This variant (3) represents the longest
            transcript and encodes the longest isoform (3).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219381.177776.1,
                                           SRR11927933.2776588.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-728               AL732626.8         106876-107603
            729-918             AL732626.8         111469-111658
            919-1126            AL732626.8         112006-112213
            1127-1260           AL732626.8         118228-118361
            1261-1529           AL732626.8         118615-118883
            1530-1855           AL732626.8         119528-119853
            1856-1949           AL732626.8         122866-122959
            1950-2073           AL732626.8         123095-123218
            2074-2216           AL732626.8         123297-123439
            2217-2395           AL732626.8         123580-123758
            2396-2542           AL732626.8         126996-127142
            2543-2659           AL732626.8         127260-127376
            2660-5298           AL732626.8         127596-130234
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 23.05 cM"
     gene            1..5298
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /note="transmembrane protein 8B"
                     /db_xref="GeneID:242409"
                     /db_xref="MGI:MGI:2441680"
     exon            1..728
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..2995
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /note="isoform 3 is encoded by transcript variant 3"
                     /codon_start=1
                     /product="transmembrane protein 8B isoform 3"
                     /protein_id="NP_001355769.1"
                     /db_xref="CCDS:CCDS89738.1"
                     /db_xref="GeneID:242409"
                     /db_xref="MGI:MGI:2441680"
                     /translation="MAQPLSRPLILSQFQPWPPATPSPRFPNLSRPRPVSQRMSRSRS
                     PLQPRSLPPAWPLSPPRPLFHPLSQFPAQHLTLSHSPRLLKPLAQPQPLLQSPSHPLL
                     PSHLLPLFEPRCSVQRHPKKAQSLPPSLCLPKSLPLGPRLSHTLPLSQPRLRSGLQLP
                     PALLLLLLFSVLGPGAGGLFLTDYSTCSPRKLSPFRSFASTELFHFHVPEDTFLAVWN
                     LIIFKEQGGTFGDHCPDQSVTVYFRSGAPPVINPLHTHFPADTAVPGVFSLTLSWTLP
                     NRTSGIFNVSSPLPGDWFLAAHLPQAHGHISVKGLQDECQYLLQPQLIVRRLLDVAVL
                     VPGRPSEQTLSSHNRSALYKVFVPSFTYRVSAQLVCVGGRGASVCPLTLRLRPKAPPL
                     HNSSSVACGGASVCQLELALPPWGHWVYVRVETPSRGPGRTISFQLCVRLQECPQPSL
                     SRALVPGAAMNMPQSLGTQPLPPEPPSLGTPIEGSGAIAPTEHCWPVRPTLRNELDTF
                     SVHFYIFFGPSVALPPERPAVFALRLLPVLDSGGVLSLELQLNASSLRQENVTVFGCL
                     THEVPLSLGDAAVTCSKESLAGFLLSVSATSRVARLRIPFPQTGTWFLTLRSLCGVGP
                     RFVRCRNATAEVRLRTFLSPCVDDCGPYGQCKLLRTHNYLYAACECKAGWRGWGCTDS
                     ADALTYGFQLLSTLLLCLSNLMFLPPVVLAIRSRYVLEAAVYTFTMFFSTFYHACDQP
                     GIVVFCIMDYDVLQFCDFLGSLMSVWVTVIAMARLQPVIKQVLYLLGAMLLSMALQLD
                     RHGLWNLLGPSLFALGILATAWTVRSVRRRHCYPPTWRRWLFYLCPGSLIAGSAVLLY
                     AFVETRDNYFYIHSIWHMLIAGSVGFLLPPRAKTDRRVPSGARARGCGYQLCINEQEE
                     LGLVGPGGTTVSSICVS"
     exon            729..918
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1126
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1260
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1529
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1530..1855
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1856..1949
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..2073
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2216
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..2395
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2396..2542
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2543..2659
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2660..5298
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      5056
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /note="major polyA site"
     regulatory      5276..5281
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5297
                     /gene="Tmem8b"
                     /gene_synonym="4930500O05Rik"
                     /note="major polyA site"
ORIGIN      
        1 gttaacgccg gttcagcgca gcccggagtc gcccaggcct gaactcctcc ccaggtcccg
       61 gccccggccc cggccccggc cccagccccg agcccgcgag gacgccagag gccacccccc
      121 gggggtgggg agcggagccg cgggtcagct ctacgagcgt cccgtgctgg cctggccggc
      181 cccgcccccg ccccgggcca tggcccagcc cttgtcccgg cccctcatcc tatcccaatt
      241 tcagccttgg cctccggcca cgccctcgcc ccgcttccca aacctgtcca ggccccggcc
      301 ggtgtcccag cgaatgtcca ggtccaggtc cccgcttcaa ccccggtccc tgcccccagc
      361 ctggccgcta tctccgcctc ggcccttgtt ccaccccttg tcacaattcc cagcccagca
      421 tctgaccctg tcccattccc cgcgtttgct taagcccctg gcccagccac agcccttgct
      481 tcaatctcca tcacacccac tccttccatc acaccttctg cccttgtttg agccccggtg
      541 ctcagttcag cgccacccaa aaaaagccca gtctttgccc ccatctctat gtttgcccaa
      601 gtctcttcca ctgggtcccc gcctctctca tactctaccc ctctcccagc ctcgactcag
      661 gtctgggctc cagctgccac cagccctatt gctgctgttg ctgttttctg tccttggtcc
      721 aggggctgga ggcctgttcc tgactgatta ctccacctgc tcgccccgca agctcagccc
      781 ctttcgctcc tttgccagta cggagctctt ccacttccac gttcctgagg acacgttcct
      841 ggctgtttgg aacctcatca tcttcaagga gcagggagga acctttgggg accactgccc
      901 agaccaaagc gtgactgtgt atttccgctc tggagcacct cccgtcatca atccgctgca
      961 cacacacttc ccagcagaca cggctgtgcc tggggttttc tcactgaccc tcagctggac
     1021 actgcccaac agaacctcag gcatcttcaa cgttagcagc cccttacctg gggactggtt
     1081 cttggctgcc caccttcccc aagcccatgg ccacatctct gtcaagggtc tccaggatga
     1141 atgtcagtac ctgcttcagc cacaactgat tgtccgacga ctgctggatg tggctgtact
     1201 ggttcctggc cgtccctcag agcaaaccct ttcctcccat aatcgttcag ccttgtacaa
     1261 ggtctttgtg ccaagcttca cctacagggt ttcagcccag ctggtgtgtg tagggggtcg
     1321 aggggcatct gtctgtcccc tgacactgcg cctacgtccc aaggctccac ccctacacaa
     1381 ctcaagctct gtggcctgtg gaggtgcctc agtatgccag ctggagttgg ccttgccccc
     1441 ctggggccac tgggtctatg tgcgtgtgga gacgccatcc cgtggtcctg gcaggaccat
     1501 cagcttccag ctctgtgtgc ggttacaaga gtgtccacaa cccagcctgt cccgtgcctt
     1561 ggtccctggg gctgccatga acatgcccca gtcactaggc acccagccac tgcctccgga
     1621 gccaccatcc ctggggactc ctatagaagg gtctggagcc atagcaccca ctgagcactg
     1681 ctggccagtg cgtccgactc tgcgcaatga gctggatacc ttctctgtcc acttctacat
     1741 cttttttggt cccagcgtgg cccttccacc tgagcgccca gctgtatttg ccctgaggct
     1801 gttgccggtg ctggacagcg ggggcgtcct tagcctggag ctccagctca atgcgagctc
     1861 tctgcgccag gaaaatgtaa cagtattcgg atgcctgact catgaggtgc ccttgagcct
     1921 gggggatgct gctgtgacct gttccaaaga gtccctggca ggcttccttc tctcggtcag
     1981 tgccacctcc agagtggcca ggcttcgaat cccattccca cagacgggga cctggttcct
     2041 gaccctccgt tctctgtgcg gggtgggacc tcggtttgtg cggtgccgca atgcaacggc
     2101 agaagtgcgg ctgcgtacct tcttgtcccc atgtgtggat gactgtgggc cctatggcca
     2161 gtgcaaactg ctgcgcaccc acaactacct gtacgcggcc tgcgagtgca aggctgggtg
     2221 gcggggctgg ggttgcacag acagtgccga cgcgctcacc tatggattcc agctgctgtc
     2281 cacactcctc ctctgcctga gcaacctcat gtttttgcca cctgtggtcc tggccattcg
     2341 gagccgatac gtgctggaag ctgccgtcta caccttcacc atgttcttct ccacgttcta
     2401 tcatgcctgt gaccagccag gcattgtggt tttctgtatc atggactatg atgtacttca
     2461 gttctgtgat ttcctgggct ccttaatgtc cgtgtgggtc accgtcatag ccatggctcg
     2521 tctgcagcct gtgatcaagc aggtgctgta tctgctcggg gctatgctgc tgtctatggc
     2581 tctgcagctt gaccggcatg gactctggaa tctgctggga cccagtctct ttgccctcgg
     2641 gatcttggcc acagcttgga cagttcgcag cgtccggcgc cggcactgtt acccaccgac
     2701 atggcgccgc tggcttttct acctgtgtcc cggcagcctg attgctggca gtgccgttct
     2761 actgtatgct ttcgtggaga ctcgggacaa ctacttctac attcacagca tttggcacat
     2821 gctcattgct ggcagtgtgg gcttcctgct gcctccccgt gccaagactg accgccgggt
     2881 cccttctgga gcccgggcca ggggctgcgg ttaccagctg tgcatcaatg aacaggagga
     2941 gctgggactt gtgggcccag gagggaccac tgtcagcagc atctgtgtca gctgagaggg
     3001 gctttgggcc aagctctaag gggacatgaa ccctgcctag ggttcctcct ggggctgtgg
     3061 agcccaccta ggtacaggga caagtagtgt ttcttaatga cacagtcttt ctcaaggatg
     3121 actgttttca gggagcagga ggctttccta agacatggag aatttcttaa gagcctagag
     3181 tcctcctgtg cttatggagt ccttcttaag gatgagctat gcaggacact gcgcccctga
     3241 ggaccaggga atccctaggg gtgtggagct cttcccaggg atagggaacc ctcctacaga
     3301 catgagatac ttcccaagga gacaaaatgt catcagtgca gagggaaaag gggtcgatcc
     3361 tggagaaaca agtcacggga tttttccagt ggcttagcag tgctgggcag aagggctgca
     3421 ggagagagag agggagagag ggagagaggg ggagagagtg agccgggact gctaggcagc
     3481 tggcgtcctc atgtggtaca gcggagcctg ctatgtctcc agtgaagtgt gtactgagtc
     3541 tgtgctgcct tttttccctt gttgggggtc agggtgtggg aaaatggggc gagagggaca
     3601 ctgaggaaat cctgagagtg acagggacct tcctggagac gatgctggaa gctactgtct
     3661 ggtttctgcc ccgtggcttg tacaccagta tccctctcat ctggtccctg tgtttaattc
     3721 caaatactcc ccaatgccca tttcccaatt gctatggatc ctagtctgga gtagcgaaga
     3781 tgggtgtgtg catctgtgtg tgcgtgtgct tgctgtgtat atacagagat gaattagtga
     3841 gacagactcc ccatgacgcc tacacccaca gagaaggaag ctgaagtgcc aggaaattag
     3901 agtaacaggt acaaatttca gcatcgaagg caccacaaga atggaactat tttaagcttc
     3961 catttttttt tcttgcatgc cagaaataat cttgtataaa ttgagatttt tttctttgtg
     4021 tgtgtgtgag tgagtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg agagagagag
     4081 aaagagagag agagagagag agagagagag agagagagag agagagagag agagagagag
     4141 agaggagaga gagaaaggag gaaagagaga gaggctctca ctatgtatat agtttgttct
     4201 ggctcagatc tcatgttcct ctgggtcctg gagttacaga catgggtgcc atacctggca
     4261 gctggacttg ttctttcccc atggggtcat agctgagttt tgagggtctt gtcttacact
     4321 gagatacgag aacattcttt ctggtcccca gtatccatac tttatcatct tacaagagtt
     4381 cagtcacccc agctggctcc cagtctgcag tcagacaggc tgctaaatgc tgagaggctt
     4441 cgtgttctgt ctgcttagcc ccgagttcct gcaccccagg tctgtgccac atggatgttc
     4501 ttgaatggct acccagacac agcatgaagc cgcttttctt ctggagtcct gtgctaggtt
     4561 ttacttgata aggcaaaaga tgagttgttt tttcttttaa gctccgtaga tggagccagg
     4621 ggctctgctc ctgtctcttt agcaacctgg gacagtgctc gcaccttctc ccctcctccc
     4681 cactcccact tgtcagactg gagaaggagc cagcctcaca tttcccccaa ttcttctctc
     4741 tgtgccttaa gtcctttcta ctctcttcac aacccaggcc tttgaagccc aaagcctaga
     4801 aaataccatt ttattcttta ctctctgtaa taaaatctct tcctgaggca ctgagtactg
     4861 aataaagtag ctgactgatt ggccaggagt gagggtatac aggctgggtg tctcttcctg
     4921 tattaaaagt gctattcttg ggccaggtat ggtggtgcat gcctttaacc ccagcattct
     4981 ggggctagga gctggtgagt taatgtgagt gagagccagc caaggttaca tagaccctct
     5041 cttaaagaaa aataaagtaa aagaaaataa aatggctgaa gaaatggccc agcaattacg
     5101 agcatgcatt gctgttccag aggacccgag ttcaattccc tgtatcctgt taccagctaa
     5161 gatgcctgta accacagctc caggggatac actacctctg ggctccatgg gtacctctcc
     5221 acacaagtgg tgtacactca cacagacaca aacacacata cacatgatta aaaataataa
     5281 atttttttca gaaagaag
//
