LOCUS       NM_028097               2690 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus transmembrane protein 68 (Tmem68), transcript variant
            1, mRNA.
ACCESSION   NM_028097 XM_973122
VERSION     NM_028097.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   McLelland,G.L., Lopez-Osias,M., Verzijl,C.R.C., Ellenbroek,B.D.,
            Oliveira,R.A., Boon,N.J., Dekker,M., van den Hengel,L.G., Ali,R.,
            Janssen,H., Song,J.Y., Krimpenfort,P., van Zutphen,T., Jonker,J.W.
            and Brummelkamp,T.R.
  TITLE     Identification of an alternative triglyceride biosynthesis pathway
  JOURNAL   Nature 621 (7977), 171-178 (2023)
   PUBMED   37648867
  REMARK    GeneRIF: Identification of an alternative triglyceride biosynthesis
            pathway.
REFERENCE   3  (bases 1 to 2690)
  AUTHORS   Tamplin,O.J., Kinzel,D., Cox,B.J., Bell,C.E., Rossant,J. and
            Lickert,H.
  TITLE     Microarray analysis of Foxa2 mutant mouse embryos reveals novel
            gene expression and inductive roles for the gastrula organizer and
            its derivatives
  JOURNAL   BMC Genomics 9, 511 (2008)
   PUBMED   18973680
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2690)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL732617.24, AK084881.1 and AK048210.1.
            
            On Sep 20, 2017 this sequence version replaced NM_028097.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK048210.1, AK166237.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                AL732617.24        173012-173064       c
            54-2554             AK084881.1         3-2503
            2555-2690           AK048210.1         2470-2605
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 1.98 cM"
     gene            1..2690
                     /gene="Tmem68"
                     /gene_synonym="2010300G19Rik; Diesl; MGAT/DGAT"
                     /note="transmembrane protein 68"
                     /db_xref="GeneID:72098"
                     /db_xref="MGI:MGI:1919348"
     exon            1..150
                     /gene="Tmem68"
                     /gene_synonym="2010300G19Rik; Diesl; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            151..202
                     /gene="Tmem68"
                     /gene_synonym="2010300G19Rik; Diesl; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            203..591
                     /gene="Tmem68"
                     /gene_synonym="2010300G19Rik; Diesl; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            592..756
                     /gene="Tmem68"
                     /gene_synonym="2010300G19Rik; Diesl; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            757..950
                     /gene="Tmem68"
                     /gene_synonym="2010300G19Rik; Diesl; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1011
                     /gene="Tmem68"
                     /gene_synonym="2010300G19Rik; Diesl; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1151
                     /gene="Tmem68"
                     /gene_synonym="2010300G19Rik; Diesl; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..2690
                     /gene="Tmem68"
                     /gene_synonym="2010300G19Rik; Diesl; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2433..2438
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem68"
                     /gene_synonym="2010300G19Rik; Diesl; MGAT/DGAT"
                     /note="hexamer: AATAAA"
     polyA_site      2454
                     /gene="Tmem68"
                     /gene_synonym="2010300G19Rik; Diesl; MGAT/DGAT"
                     /note="major polyA site"
     regulatory      2461..2466
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem68"
                     /gene_synonym="2010300G19Rik; Diesl; MGAT/DGAT"
                     /note="hexamer: AGTAAA"
     polyA_site      2492
                     /gene="Tmem68"
                     /gene_synonym="2010300G19Rik; Diesl; MGAT/DGAT"
ORIGIN      
        1 ggggcgaccg acggtcctac acccaatgtg gctcggcctt cttcctctca ggcagttaaa
       61 ggaaaccggc actacccgcg tgtctgcttc tcccggctct gccgccgacc agctgccgag
      121 gtcgccgatt tcctgtgact ttcttgtcag agtatatgca ttgaagctaa agacaagata
      181 tttgtgtaat ttgtgtaatc tggcattgaa gttggaacat atggcaatga agaaagagac
      241 aggatttggg attttcatct aagaacatga tagataacaa ccaaacctgt gctgcaggac
      301 aggactctgt gccctatgtg acctgtatga tttatgtgct tgaggagtgg ttgggtgtgg
      361 agcaattgga ggactatctg aattttgcaa accatctttt gtgggttttc accccactga
      421 tacttttaat acttccttat tttaccatct ttcttctcta ccttactatt attttcctcc
      481 atatctacaa gaggaagaat gtattaaaag aagcctactc tcataacttg tgggatggtg
      541 caaggaaaac ggtggcaact ctgtgggatg gacatgcggc tgtttggcat ggttatgaag
      601 ttcatgggat ggaaaagata ccagaaggag ctgcacttat aattttttat catggagcca
      661 tccccataga cttttactac ttcatggcta aaattttcat ccagaaaggc agaacttgcc
      721 gagtagtagc tgaccacttt gtctttaaaa ttccagggtt cagtttatta cttgatgtgt
      781 tttgtgctct tcatggacca agagaaaaat gtgttgaaat cttgaggagt ggtcacttgt
      841 tagctatctc accgggcgga gttcgagaag ccttacttag tgatgaaacc tacaacatca
      901 tatggggtaa tcgtaaaggc tttgctcagg ttgcaattga tgcaaaagtg cccattattc
      961 ctatgtttac acaaaatatt cgagaaggat ttagatcact tggaggcaca agattattta
     1021 agtggcttta tgaaaaattc cgctatccat ttgctccaat gtatggaggt tttcctgtaa
     1081 agttgcggac cttcttaggt gatcccatac catatgaccc aaaggtaaca gcagaagaat
     1141 tagccgaaaa gaccaagaat gctgttcaag ctttgattga caagcaccaa aggataccgg
     1201 ggaacattag gagtgctctg ctggaccgct ttcataaaga gcagaaggct cattagcaga
     1261 tagcttaatg tgtctgcagt gacaagtttg catctgtggt actgtcttgt aggttgtata
     1321 ttgattagtg tttttaaaac gttgtgctaa tgaacatctt tctttagaac ttaaacttta
     1381 tatggttaat cttatccttt caatcagttg tgtcaaattt aataagtagc tcatcatttg
     1441 cttgactcag caaatgatca tgttgcattt gcagtttttt tagtagtata tgataagcac
     1501 agaatcttag agttaagtct tccttggtat ccgcagatta ggtcaggcag actttactaa
     1561 atcatgtttc ttatgtttgt gtctgttgtg ccaatatgat agtagggtgt agcacttgct
     1621 taattaagat gagtgatgcc tttatgtatc tgtccttcat tccattcaca actgctggca
     1681 agttgtggaa ctcttttgca cctcaatgat ttatgttaag catgctaata ctgtgctgat
     1741 gaacattatt ttaagttgct tatatctact gtgatatatc catatatgat agatatctgt
     1801 agtgagaatc tataattgaa ttgattccta aatcctctag gagttatgta tataattttg
     1861 tccattggat gttgaaacta ctccttcagg tatgatgtcc agttgccaag cacattgaaa
     1921 gagatgtact attttataaa tcaaatcatt gttatttgta gtatttatta accgaattgt
     1981 ttagtattta ttttaaataa gtatctaagg aaatgtatag gatgagttgt cagcacagtg
     2041 ttttatggtg taaagacaaa atcatgggca tgctctgcag ttcatgttag aagtgggttc
     2101 taggctaagg aataacatgc taagacagct ctggtctcgc tcagtatccg ggctctcttc
     2161 ctgtgctttg cagccagtaa cgtggttatt attttaataa aacgtacttg aatgataaac
     2221 ttctgtaaac atggagctct ctgaaaatgt aattgctttt gatttgttgt ctttttgaaa
     2281 acttatttta aaagtattta aacaacaaag tatcataagc tcactgcagt atgtctgtct
     2341 cctaccacct ttgtatactt gggttttata tggacttttt aactacaagt gtttatttat
     2401 gacatatata atgtatatag aaatgatgtg aaaataaagc tgaattgcac ctccttctct
     2461 agtaaaaatg ttattcttga gattcttcaa aattgtctcc atacgtctca gtagtcattt
     2521 cttttgacca caataaatat gtggtatacg tcctatgtag tctgtatatt tcagggacaa
     2581 cttcagagaa aggagccaat ttgctgccac tccctctcac tttagtaaat ggtacttggt
     2641 tcaaagctgt taaatatttc caaaatgaag gaataaaagt ctataaaatg
//
