LOCUS       NM_175212               3644 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus transmembrane protein 65 (Tmem65), mRNA.
ACCESSION   NM_175212
VERSION     NM_175212.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Teng,A.C.T., Gu,L., Di Paola,M., Lakin,R., Williams,Z.J., Au,A.,
            Chen,W., Callaghan,N.I., Zadeh,F.H., Zhou,Y.Q., Fatah,M.,
            Chatterjee,D., Jourdan,L.J., Liu,J., Simmons,C.A., Kislinger,T.,
            Yip,C.M., Backx,P.H., Gourdie,R.G., Hamilton,R.M. and
            Gramolini,A.O.
  TITLE     Tmem65 is critical for the structure and function of the
            intercalated discs in mouse hearts
  JOURNAL   Nat Commun 13 (1), 6166 (2022)
   PUBMED   36257954
  REMARK    GeneRIF: Tmem65 is critical for the structure and function of the
            intercalated discs in mouse hearts.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3644)
  AUTHORS   Sharma,P., Abbasi,C., Lazic,S., Teng,A.C.T., Wang,D., Dubois,N.,
            Ignatchenko,V., Wong,V., Liu,J., Araki,T., Tiburcy,M., Ackerley,C.,
            Zimmermann,W.H., Hamilton,R., Sun,Y., Liu,P.P., Keller,G.,
            Stagljar,I., Scott,I.C., Kislinger,T. and Gramolini,A.O.
  TITLE     Evolutionarily conserved intercalated disc protein Tmem65 regulates
            cardiac conduction and connexin 43 function
  JOURNAL   Nat Commun 6, 8391 (2015)
   PUBMED   26403541
  REMARK    GeneRIF: Tmem65 is an intercalated disc protein that interacts with
            and functionally regulates ventricular Cx43.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3644)
  AUTHORS   Miller,J.A., Nathanson,J., Franjic,D., Shim,S., Dalley,R.A.,
            Shapouri,S., Smith,K.A., Sunkin,S.M., Bernard,A., Bennett,J.L.,
            Lee,C.K., Hawrylycz,M.J., Jones,A.R., Amaral,D.G., Sestan,N.,
            Gage,F.H. and Lein,E.S.
  TITLE     Conserved molecular signatures of neurogenesis in the hippocampal
            subgranular zone of rodents and primates
  JOURNAL   Development 140 (22), 4633-4644 (2013)
   PUBMED   24154525
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC107772.19.
            
            On Dec 12, 2006 this sequence version replaced NM_175212.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC096426.1, SRR17784649.239632.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-585               AC107772.19        133814-134398       c
            586-630             AC107772.19        108172-108216       c
            631-698             AC107772.19        105313-105380       c
            699-753             AC107772.19        101126-101180       c
            754-796             AC107772.19        100357-100399       c
            797-902             AC107772.19        98081-98186         c
            903-3644            AC107772.19        93240-95981         c
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 25.09 cM"
     gene            1..3644
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
                     /note="transmembrane protein 65"
                     /db_xref="GeneID:74868"
                     /db_xref="MGI:MGI:1922118"
     exon            1..585
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             300..1004
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
                     /codon_start=1
                     /product="transmembrane protein 65"
                     /protein_id="NP_780421.2"
                     /db_xref="CCDS:CCDS27493.1"
                     /db_xref="GeneID:74868"
                     /db_xref="MGI:MGI:1922118"
                     /translation="MSRLLPLLGSRTARSLRPGPAAAPRLPSWCCCGRGLLALGVPGG
                     PRLLGTHPKKEPMEALNTAQGARDFIYSLHSTERSCLLKELHRFESIAIAQEKLEALP
                     PTPGQLRYVFFHNAIPFVGFGFLDNAIMIVAGTQIELSIGIILGISTMAAAALGNLVS
                     DLAGLGLAGYVEALASRLGLSIPDLTPKQVDMWQTRVSTHLGKAVGVTIGCILGMFPL
                     IFFGGSEEDEKLETTN"
     exon            586..630
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            631..698
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            699..753
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            754..796
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            797..902
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            903..3644
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1362
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
                     /note="major polyA site"
     regulatory      3100..3105
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3121
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
                     /note="major polyA site"
     regulatory      3507..3512
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
                     /note="hexamer: AACAAA"
     polyA_site      3528
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
     regulatory      3623..3628
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3644
                     /gene="Tmem65"
                     /gene_synonym="2610029O13Rik; 4930438D12Rik"
ORIGIN      
        1 cgccgccgct cctcatccgg gaggcgcccg cccgcccgca ccgcccgccc gctccgagct
       61 cccggcccgg ccgcggcgca gaaagttttc ccgggctcac ggcctgcggg aagcggagcg
      121 ggcacggctg ggaggcggtc gccgcgcggg cccgccgagt gcatggtgtc gggcgcccgg
      181 gccgcgcgtc aggaggaccc ggggcggggc cgggctgagc ccagctcctg agcctctgtc
      241 cagctgcgcc ccgaagccgt cttcccttgg gaccgctgcg ggggtcgcgc tccagcgcca
      301 tgtcccggct gctgccgctg ctggggagcc ggacggcgcg cagcctgagg cccggccctg
      361 ccgccgcgcc ccgcctgccg tcctggtgct gctgcggccg gggactgctg gcgctcgggg
      421 ttcccggcgg tccccggctt ctgggcacac accccaagaa ggagcccatg gaggcgctga
      481 acacggcgca gggcgcgcgc gacttcatct acagcctgca ctccacggag aggagctgct
      541 tgctcaaaga gctgcaccgc ttcgagtcca ttgccatcgc acaagaaaaa ttggaagctc
      601 taccacccac tccaggacag ctgagatatg tattcttcca caatgcgata cctttcgtag
      661 ggtttggctt tttggataac gcaattatga ttgttgcagg aacccaaatt gaattgtcta
      721 ttggaattat cttggggatt tcaacaatgg cagctgctgc tttgggaaat cttgtttcag
      781 atttagctgg actgggcctt gcaggttatg ttgaggcctt ggcgtccagg ctaggcctct
      841 caattcctga tcttacacca aagcaagttg acatgtggca aacacgtgtt agcacacatt
      901 tgggcaaggc tgttggggtg acgatcggct gcattctagg aatgttcccg ttgattttct
      961 ttggaggaag tgaagaggat gagaaactgg aaacaacaaa ttaatcacct tagaacacct
     1021 ataagaagat gtaaactaat gtacctcatc attaactata ctgtccccac agttagcagt
     1081 gaagacagta gcagtgtata gatacgggat caaataactc agcatgtact atggaaacac
     1141 taacttattg tggcttggtc ttcttaagac atctttttaa acttacttag tatcatttta
     1201 tgtaaattgt tgatgctttc catcaatggc agtcgacatg tcatcttttc cttctctgtg
     1261 tatcaagata ttatttaact ttcagtcact gatactgtac tgtactgtac tgttctgact
     1321 tgggtttgca tatttgttac ataacatgca cgggaatacc tatttatggt tttattttta
     1381 tttttcctat ggttatatca atgctgcggt ttctccaaat tatttaagat gtttaatatc
     1441 agttaacatt ttaactccct gtctggtggt attacttctg tgttaattca cagtgaacat
     1501 ttacaatcat ataaattcat tcaattttta acctttcaca tctatccaaa atgaagcagt
     1561 gttttaagta tgtctgattc actttaattt tgagccatgc tgtgttaaga gccagaggtt
     1621 aatcggatgg aacaattctc tggcctccct tttacacaaa ccatacatgc taacaagtgt
     1681 acttatctct gattggctct ttacctttaa tttcccaact ttgaacaatc acacagtgaa
     1741 ttctggtttg tgagagaaag gcagtgcttg gatatgtccg gtctcttaaa atagggatta
     1801 agagagtagg gaaaactaag aaatttataa tgacatttgg aaattaggct aaaataattt
     1861 tttttttata gaaaatggaa attttcaagg aggttgtgtg gggtggatga taaagttcca
     1921 gtgctgccgg gaaggttcag gaagcatcag ttctatggct ttggaaagag tttcccctga
     1981 tgtgttgtca ttgtctcatg gaacggaagg agccaaaata tcccatgctt aatcctaatc
     2041 tcattagatc ttgctttgtg acttttttct ggatgaatat tttagaaaca acatgtattt
     2101 tctagtttgc tacctagtct gtaacacaag gagaggtcct ttctcccttt ggtggatggt
     2161 tttcttcttt cgtggtggtt atgcacacag ttggtgaaag ctgtgtgtgt gtgtgtgtgt
     2221 gtgtgtgtgt gtgtgtgaca gctgtgtcct gtgtctgacc tgaacaccgt cctgagaatc
     2281 ccgagcttta attgctgaac tgcccccccc ctttatctca tttgctctac atgttaaaaa
     2341 gttatatgta gcagaataat tggacatgtg gaagagagaa actgaagtta gaatttaatc
     2401 cttggggaat taaaattatt catgtttcct aagtagatgg aaaagatgaa cttcacgtga
     2461 ctatgctttc taaatcttat tttggagttt tgtttgcaca taattacttt gggctccttt
     2521 taaagaaaat ttcttttcca taagaaagca tgtcatatat tgttttcttt gcactttgtc
     2581 ctgtccattt atcatagaat ttaaggaaag atagcataaa agtaaaaaac atcaaattct
     2641 catatacaca agtaatattc tcctttggtc aggctagcat cacatgactt gaggtggcag
     2701 acagattcat ctttgctcac atgtcccacg acagcaagca caaggttcca ggtgaaaggc
     2761 tagaatgcac attttacttt ctgtgtagac atgtgagtgc tctttccttc ccttgtgtat
     2821 cggtggcatg tcctgtcttt gtttgtcagt gctgtattgt ggtgcttaac tcttctttgt
     2881 attaaatacc atctccagaa acttgtcttg gagtttagaa agcactttgg attgttgatt
     2941 ttttttttct ttctcttgaa aaagctcctc gtccttatta ccagaatttc tgttaacaga
     3001 attatgccag ttttgttgaa aacttcactt atgggaggtg taactttcac acgaaagaca
     3061 tgctaatttt cagtcaaaat ttttgaatca tatttaatca ataaaatgct ttcaatcatt
     3121 atgatacttc gttttcaaaa gcagacaaac aaaattaaag tctcgttctc tcgagctctg
     3181 gctctgacct aacatggtac tttgcacagt gtgatttctg aaggtggccc ctttaagtta
     3241 aggactccat accacccgtg ttgagcgttt ttcaccacag agactctgat gccagctgaa
     3301 actggactgc cctctttctc atagcagctg ttctctatca gcgttagagc tgcagcggac
     3361 ggcatcgtgc gctgctgtag tcctgtctgt tcagaatcca tggtgtttgt gtattatgat
     3421 ttatggactt gttgatgcat gtaaactggg tgcattctgt tgcctctgta tgttaaatag
     3481 tgaccaatgt ttttacgaaa gaattgaaca aaaaaatatc ttttaagacg tttggaatgt
     3541 ggtttttgat tttaaaattt ggttttctta ttaatctcag aggcgctggg ttattctatg
     3601 acagaaggaa acaggatcaa tgaataaatg caggtttgta atga
//
