LOCUS       NM_001361704            5299 bp    mRNA    linear   ROD 31-JUL-2024
DEFINITION  Mus musculus transmembrane protein 63c (Tmem63c), transcript
            variant 3, mRNA.
ACCESSION   NM_001361704 XM_006515716
VERSION     NM_001361704.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Qin,Y., Yu,D., Wu,D., Dong,J., Li,W.T., Ye,C., Cheung,K.C.,
            Zhang,Y., Xu,Y., Wang,Y., Shi,Y.S. and Dang,S.
  TITLE     Cryo-EM structure of TMEM63C suggests it functions as a monomer
  JOURNAL   Nat Commun 14 (1), 7265 (2023)
   PUBMED   37945568
  REMARK    GeneRIF: Cryo-EM structure of TMEM63C suggests it functions as a
            monomer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5299)
  AUTHORS   Zhao,X., Yan,X., Liu,Y., Zhang,P. and Ni,X.
  TITLE     Co-expression of mouse TMEM63A, TMEM63B and TMEM63C confers
            hyperosmolarity activated ion currents in HEK293 cells
  JOURNAL   Cell Biochem Funct 34 (4), 238-241 (2016)
   PUBMED   27045885
  REMARK    GeneRIF: TMEM63 proteins may serve as an osmolarity sensitive ion
            channel for the osmoreception.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY239104.1, AK034410.1, AC132609.3 and CN459916.1.
            
            On Mar 29, 2018 this sequence version replaced XM_006515716.3.
            
            Transcript Variant: This RefSeq record was created from transcript
            and genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK043536.1, AK034410.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN00849388
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-201               BY239104.1         2-202
            202-3254            AK034410.1         2-3054
            3255-3449           AK034410.1         3056-3250
            3450-4965           AC132609.3         70596-72111
            4966-5299           CN459916.1         1-334
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 41.21 cM"
     gene            1..5299
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /note="transmembrane protein 63c"
                     /db_xref="GeneID:217733"
                     /db_xref="MGI:MGI:2444386"
     exon            1..299
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            300..362
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            363..572
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             429..2837
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /note="isoform 1 is encoded by transcript variant 3;
                     calcium permeable stress-gated cation channel 1"
                     /codon_start=1
                     /product="calcium permeable stress-gated cation channel 1
                     isoform 1"
                     /protein_id="NP_001348633.1"
                     /db_xref="CCDS:CCDS26069.1"
                     /db_xref="GeneID:217733"
                     /db_xref="MGI:MGI:2444386"
                     /translation="MSAFPDSMDQKFHNMTVNECFQSRSTVLQGQPFGGIPTVLVLNI
                     ILWVFVVLLYSFLRKAAWDYGRLALLIHNDSLTSLIYGEQSEKSSPSEVSLEAERRDR
                     GFSSWFFNSLTMRDRDLINKCGDDARIYITFQYHLIIFVLILCIPSLGIILPVNYIGT
                     VLDWNSHFGRTTIVNVSTESKFLWLHSLFAFLYFLINLAFMGHHCLGFVPKKSLHFTR
                     TLMITYVPTEIQDPEIISKHFHEAYPGCVVTRVHFCYDVRNLIDLDDQRRHAMRGRLY
                     YTAKAKKTGKVMIKTHPCSRLCFCKCWTCFKEVDAEQYYSELEEQLTDEFNAELNRVQ
                     LKRLDLIFVTFQDARTVRRIYDDYKYIHCGRHPKQSSVTTIVKNYHWRVAHAPHPKDI
                     IWKHLSIRRFSWWTRFIAINTFLFFLFFFLTTPAIIINTIDIYNVTRPIEKLQSPIVT
                     QFFPSVLLWAFTVTMPLLVYLSAFLEAHWTRSSQNLIIVHKCYIFLVFMVVILPSMGL
                     TSLHVFLRWLFDIYYLEHATIRFQCVFLPDNGAFFINYVITAALLGTGMELMRLGSLC
                     TYCTRLFLSKSEPERVHIRKNQATDFQFGREYAWMLNVFSVVMAYSITCPIIVPFGLL
                     YLCMKHITDRYNMYYSYAPTKLNAQIHMAAVYQAIFAPLLGLFWMLFFSILRVGSLHS
                     ITLFSMSSLIISVVIAFSGVFLGKLRIAQRYEQPEEETETVFDVEPSSTTSTPTSLLY
                     VATVLQEPELNLTPASSPARHTYGTINSQPEEGEEESGLRGFARELDSAQFQEGLEME
                     GQSH"
     exon            573..652
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            653..734
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            735..772
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            773..915
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            916..964
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1073
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1147
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1228
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1352
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1486
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..1609
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1763
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1870
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1871..1957
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1958..2023
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2024..2186
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2187..2292
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2293..2460
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2461..2570
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2571..2642
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2643..5239
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3424..3429
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      3458
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /note="major polyA site"
     regulatory      5221..5226
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5239
                     /gene="Tmem63c"
                     /gene_synonym="4932420N09; 9330187M14Rik"
ORIGIN      
        1 agttgttgca aaccgcagcc agggagcggc agccttgtgc attcgcgatg gtcatggttt
       61 ctgataacta caggatttcc agggcagagc cttggtccct gcttatctag cccgggttcc
      121 cccacaggtc agagcagcgt tgggcggggc caaggttgac tcctactggg aggagccggg
      181 atgccgctcc cgcggtgatg cagaggggaa tttgctgaca gtcccttgcc aggtggagct
      241 ggctttacca ggccgctaaa aagaaggagg aggaggaccc acccgcagtc tcctctcagc
      301 aaagctgttg ctgagtggat gcctcagatc tgacgtggac cttccctgga gagctgcctc
      361 agagtgggga gtagtcccag tgtctgactg agaccttctg cctggctggg agcctccgtg
      421 tgcccaggat gtctgccttt ccagacagca tggaccagaa gtttcataac atgacggtga
      481 atgagtgctt ccagtcgcgg agcactgtcc tccaggggca gccctttggg gggatcccca
      541 ccgtgctcgt actcaacatc atcttatggg tgttcgttgt cctgctttat tccttcctcc
      601 ggaaagctgc gtgggactac gggaggctgg cactgctgat acacaacgac agcctgacct
      661 cactgatcta cggggagcaa agtgagaagt catctccgtc agaggtttcc ttggaggcgg
      721 aacgcaggga caggggcttc tcttcctggt tcttcaacag cctaaccatg agggaccggg
      781 acctgatcaa caaatgcggg gatgatgcgc gaatttacat cacgttccag tatcacctca
      841 tcatctttgt gctcatcctc tgcatcccct ccctgggcat cattttgcct gttaactaca
      901 ttggaactgt tctggactgg aacagtcact ttggccggac caccattgtc aatgtctcta
      961 cagagagcaa gttcctgtgg ctgcatagcc tcttcgcgtt cttgtacttc ctcatcaacc
     1021 ttgccttcat gggtcatcac tgcttgggat ttgtgccaaa gaagagcctc cacttcacga
     1081 ggacactgat gatcacctat gtacccactg aaatccagga tcccgagatc atcagcaagc
     1141 acttccatga ggcctatcct ggatgtgtgg tgaccagagt ccatttctgc tatgatgtca
     1201 ggaacctgat tgacttggat gatcaaaggc gccatgccat gcggggcagg ctctactaca
     1261 cggccaaggc taagaagact gggaaggtca tgatcaagac acatccctgt tcccgcctgt
     1321 gcttctgcaa atgttggacc tgcttcaagg aggtagatgc ggagcagtac tacagtgagc
     1381 tggaggagca gctgacagac gagttcaatg ctgagcttaa tcgtgtccag ctgaagaggc
     1441 ttgacctgat ctttgtcacc ttccaggatg ccaggacagt gagacgcatc tatgatgatt
     1501 acaagtacat ccattgtggt agacacccca agcagtcttc agtcacgacc atcgtcaaaa
     1561 actaccactg gagggttgct catgcccctc accccaaaga catcatctgg aagcacctgt
     1621 ccatccgccg cttcagttgg tggactcgct ttatcgccat caataccttc ctgttcttcc
     1681 tcttcttctt tctcaccaca cctgccatca tcatcaacac catcgacata tacaatgtca
     1741 cccgccccat tgagaaattg cagagcccaa tcgtgactca gttcttcccc tctgtactgc
     1801 tctgggcctt cactgtgaca atgcctctgc tggtctactt atcggccttc ctagaggccc
     1861 actggacaag atcaagtcag aacctgatca tagtgcacaa atgctacatc ttcctggtgt
     1921 ttatggtggt cattcttccc tccatggggc tgaccagtct gcatgtgttt ttacgatggc
     1981 tctttgacat ctactatctt gaacatgcga ccatcaggtt ccagtgcgtg ttcctcccag
     2041 acaatggtgc attcttcatc aactacgtga tcacagcggc tctgcttggg acaggcatgg
     2101 agctgatgcg cctggggtca ctctgcacat attgtacccg cctcttctta tccaaatcag
     2161 agccagaaag agttcacatc agaaagaacc aagccacaga tttccagttt ggacgagagt
     2221 atgcatggat gttgaacgtc ttcagcgtgg tgatggcgta tagcatcact tgccctatca
     2281 ttgtcccgtt tgggctgctg tatctgtgca tgaagcacat tacagaccgc tataacatgt
     2341 actactccta cgcgcccacc aaactcaacg cgcagatcca catggccgca gtctaccagg
     2401 ccatttttgc cccactcttg gggctcttct ggatgctctt cttctccatc ctgcgagtag
     2461 gttccctcca cagcatcacc ttattctcca tgtcttccct catcatctcc gtggtcattg
     2521 ccttttctgg tgtttttctg gggaagctcc ggattgccca acggtatgag cagcctgagg
     2581 aagaaacaga gactgtgttt gatgtggaac caagcagcac cacctccact cccacctccc
     2641 ttctgtacgt ggccaccgtg ctacaggagc cagagctgaa cctgaccccc gcctcctccc
     2701 cagcccggca cacctatggc accatcaaca gccagccaga agagggagaa gaagagagcg
     2761 gtctgagggg ctttgcaagg gagctggact cagcccagtt ccaggaaggg ctggaaatgg
     2821 agggccagag tcactgatga ggactccagt ccaggggcaa ggggagagcc tggcaggggg
     2881 aggcagtaag gagcttcctt ccacagactt tgggcagctc ctgatggaga catctgccac
     2941 cctgtggccc tgtgcagagc ctaatctgct gaccagccgg aaagggaagg atggggtgga
     3001 ggaggcttat accatgtttc tgggagtggt atctaaagcc caggacacaa gagactgtct
     3061 gctgtgtgtc ttgctggggt caagctgatg agagtgtgcg tacgggaaga gataggatga
     3121 agcccactga cagcttcctg ggggttcacc atccctaccc cactccagct gcccatcaag
     3181 gattgctcca gacccctatc ctccactgca gtctgagcag aaaaaggtat gtgtactggg
     3241 ccttgtgaac aaggcaccag tggctgtctc gtcacaggtt tccctacaga gcatctagaa
     3301 actctgtact tcgccctctg atcaaggggc agctggaaca agcctgggaa gtgactgagg
     3361 ccttctgtgt ttctctgctc tgctatctac ttatcatggt agctgggaca gctactcagg
     3421 ctcattaaag gagacctaca tgcatgtggt aaagaaaacc atgctgaatc cttgctggtt
     3481 gatttaaggg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgta ggggtggcat
     3541 cgaggagttc aggaaacaag gtcttctctc aaaaactggg ccatggaaat cagacacaca
     3601 tctctctgtc ccaaggataa atgaaaagat cccagagcct agaggtagcc accaagacac
     3661 cctctgcccc atgtatgtga gtgctgtgcc ctagcatggc caagagtggt gacagcgtag
     3721 gctgcaaact gaaagcactg gccatcccct accctcagga ggactcttcc tctttcacgc
     3781 tgccatgcct ggcctctagt gtgatattgg ctcaggaatt atggatgctt cgcacagagg
     3841 tggtgtcacc ccagtcctac cagggtagga cttactcagg ggaggagtga tggatgaccc
     3901 cccaccccca cccccacaga gaagaccctt ctgatgggca agcctctggc tggaggagca
     3961 aaccccggaa atgctcagct cctcgttcca acttctgcct cttgaaataa ggcattgtca
     4021 ggaccgccat ggagcctgag ccacctgaac caaggggtct gtagcccctg aagttcctcc
     4081 ccagatatct gggttcttcc ttgctgcctc tttactgcag gtgcgtccag actcattcag
     4141 ctccaagtgt ctccacccat ggctgccctg aaaacagatc acttggcctg gtctctgctc
     4201 ccactccagc cacgtaggca gttccaagct tcactccagg gcagaaacag ttgctcaagg
     4261 agcaggtaga gtcacacccc agttcttagc cccacacgct agcatgggtg atctttgctg
     4321 agaacttgag tcagctggga aagaggctac ctccagtcca tgtcaagctt ggaataaaga
     4381 tagaactata ttcagccagg ccctatgcac tccagtatgc cgaggccctc accccgggga
     4441 tttcatcaat gaggggaaga acttcttcat agctgagtga tcaggcccaa catctagctc
     4501 tgctcaaagt ggggagttac agtccacccc cacccccaca ttgtgtattt ggtatgtagt
     4561 gatttggaat gcaaccagaa cctagatcgg ggcctcttct ctgggtgctg cttgctggca
     4621 cctgccccag atccctgcag ccctctctgc tgctgctggc cctccctcct accctgaccc
     4681 actgtagtcc atgaaagctt caggtacaag gggtcctgta gacagtaggg caggatggct
     4741 ggagggcctt tcccctctga atctgggtca gatgcagcct ggttctaatg ccaatgttgg
     4801 gcaagtggta gatcccactc tgcccttcca ggagtgtcca cctggctaga ccttgagttt
     4861 gcaaggtcac tagaggctct cctcagcatc tgctatccca gcctattggt catcaccaca
     4921 ccctggcccc atgctcagtc catcactttg atcccttctg gagggcgatg tctcccagga
     4981 gacccggaat gacacagagc ctctcagagt ggggactctt atcaggctta gagcagcctc
     5041 acccctgcct gcctgtccct aagctgcctc tggaagcaaa gtgcctatca acagcattgc
     5101 atccttgggg gtcgggaggg ggggggtgct ggaggggtgt ggactaatca cggctactac
     5161 cactaaagga atgtgccaga atactgaacc ttcttgttat taatgtatga ctgtgccttg
     5221 aataaacaag tcctcctaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     5281 aaaaaaaaaa aaaaaaaaa
//
