LOCUS       NM_001413623            3476 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus transmembrane protein 63b (Tmem63b), transcript
            variant 3, mRNA.
ACCESSION   NM_001413623 XM_006524136
VERSION     NM_001413623.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Chen,G.L., Li,J.Y., Chen,X., Liu,J.W., Zhang,Q., Liu,J.Y., Wen,J.,
            Wang,N., Lei,M., Wei,J.P., Yi,L., Li,J.J., Ling,Y.P., Yi,H.Q.,
            Hu,Z., Duan,J., Zhang,J. and Zeng,B.
  TITLE     Mechanosensitive channels TMEM63A and TMEM63B mediate lung
            inflation-induced surfactant secretion
  JOURNAL   J Clin Invest 134 (5), e174508 (2024)
   PUBMED   38127458
  REMARK    GeneRIF: Mechanosensitive channels TMEM63A and TMEM63B mediate lung
            inflation-induced surfactant secretion.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Dalghi,M.G., DuRie,E., Ruiz,W.G., Clayton,D.R., Montalbetti,N.,
            Mutchler,S.B., Satlin,L.M., Kleyman,T.R., Carattino,M.D., Shi,Y.S.
            and Apodaca,G.
  TITLE     Expression and localization of the mechanosensitive/osmosensitive
            ion channel TMEM63B in the mouse urinary tract
  JOURNAL   Physiol Rep 12 (9), e16043 (2024)
   PUBMED   38724885
  REMARK    GeneRIF: Expression and localization of the
            mechanosensitive/osmosensitive ion channel TMEM63B in the mouse
            urinary tract.
REFERENCE   3  (bases 1 to 3476)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 3476)
  AUTHORS   Zheng,W., Rawson,S., Shen,Z., Tamilselvan,E., Smith,H.E.,
            Halford,J., Shen,C., Murthy,S.E., Ulbrich,M.H., Sotomayor,M.,
            Fu,T.M. and Holt,J.R.
  TITLE     TMEM63 proteins function as monomeric high-threshold
            mechanosensitive ion channels
  JOURNAL   Neuron 111 (20), 3195-3210 (2023)
   PUBMED   37543036
REFERENCE   5  (bases 1 to 3476)
  AUTHORS   Du,H., Ye,C., Wu,D., Zang,Y.Y., Zhang,L., Chen,C., He,X.Y.,
            Yang,J.J., Hu,P., Xu,Z., Wan,G. and Shi,Y.S.
  TITLE     The Cation Channel TMEM63B Is an Osmosensor Required for Hearing
  JOURNAL   Cell Rep 31 (5), 107596 (2020)
   PUBMED   32375046
REFERENCE   6  (bases 1 to 3476)
  AUTHORS   Marques,M.C., Albuquerque,I.S., Vaz,S.H. and Bernardes,G.J.L.
  TITLE     Overexpression of Osmosensitive Ca2+-Permeable Channel TMEM63B
            Promotes Migration in HEK293T Cells
  JOURNAL   Biochemistry 58 (26), 2861-2866 (2019)
   PUBMED   31243992
REFERENCE   7  (bases 1 to 3476)
  AUTHORS   Murthy,S.E., Dubin,A.E., Whitwam,T., Jojoa-Cruz,S., Cahalan,S.M.,
            Mousavi,S.A.R., Ward,A.B. and Patapoutian,A.
  TITLE     OSCA/TMEM63 are an Evolutionarily Conserved Family of Mechanically
            Activated Ion Channels
  JOURNAL   Elife 7, e41844 (2018)
   PUBMED   30382938
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 3476)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   9  (bases 1 to 3476)
  AUTHORS   Zhao,X., Yan,X., Liu,Y., Zhang,P. and Ni,X.
  TITLE     Co-expression of mouse TMEM63A, TMEM63B and TMEM63C confers
            hyperosmolarity activated ion currents in HEK293 cells
  JOURNAL   Cell Biochem Funct 34 (4), 238-241 (2016)
   PUBMED   27045885
  REMARK    GeneRIF: TMEM63 proteins may serve as an osmolarity sensitive ion
            channel for the osmoreception.
REFERENCE   10 (bases 1 to 3476)
  AUTHORS   Hutchins,J.R., Toyoda,Y., Hegemann,B., Poser,I., Heriche,J.K.,
            Sykora,M.M., Augsburg,M., Hudecz,O., Buschhorn,B.A., Bulkescher,J.,
            Conrad,C., Comartin,D., Schleiffer,A., Sarov,M., Pozniakovsky,A.,
            Slabicki,M.M., Schloissnig,S., Steinmacher,I., Leuschner,M.,
            Ssykor,A., Lawo,S., Pelletier,L., Stark,H., Nasmyth,K.,
            Ellenberg,J., Durbin,R., Buchholz,F., Mechtler,K., Hyman,A.A. and
            Peters,J.M.
  TITLE     Systematic analysis of human protein complexes identifies
            chromosome segregation proteins
  JOURNAL   Science 328 (5978), 593-599 (2010)
   PUBMED   20360068
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC165259.2.
            
            On Nov 3, 2022 this sequence version replaced XM_006524136.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.1225831.1,
                                           SRR13948564.1137335.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-340               AC165259.2         168064-168403
            341-523             AC165259.2         174605-174787
            524-603             AC165259.2         175003-175082
            604-642             AC165259.2         175318-175356
            643-733             AC165259.2         176303-176393
            734-771             AC165259.2         178896-178933
            772-914             AC165259.2         180058-180200
            915-966             AC165259.2         180289-180340
            967-1075            AC165259.2         180478-180586
            1076-1146           AC165259.2         181089-181159
            1147-1227           AC165259.2         184296-184376
            1228-1351           AC165259.2         186501-186624
            1352-1485           AC165259.2         187608-187741
            1486-1623           AC165259.2         187865-188002
            1624-1777           AC165259.2         188139-188292
            1778-1884           AC165259.2         189048-189154
            1885-1971           AC165259.2         189314-189400
            1972-2037           AC165259.2         189510-189575
            2038-2200           AC165259.2         190650-190812
            2201-2306           AC165259.2         191351-191456
            2307-2474           AC165259.2         192395-192562
            2475-2590           AC165259.2         192750-192865
            2591-2671           AC165259.2         193016-193096
            2672-3476           AC165259.2         193339-194143
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 22.59 cM"
     gene            1..3476
                     /gene="Tmem63b"
                     /note="transmembrane protein 63b"
                     /db_xref="GeneID:224807"
                     /db_xref="MGI:MGI:2387609"
     exon            1..340
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            341..523
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     CDS             365..2863
                     /gene="Tmem63b"
                     /note="isoform 1 is encoded by transcript variant 3;
                     CSC1-like protein 2"
                     /codon_start=1
                     /product="CSC1-like protein 2 isoform 1"
                     /protein_id="NP_001400552.1"
                     /db_xref="GeneID:224807"
                     /db_xref="MGI:MGI:2387609"
                     /translation="MLPFLLATLGTAALNSSNPKDYCYSARIRSTVLQGLPFGGVPTV
                     LALDFMCFLALLFLFSILRKVAWDYGRLALVTDADRLRRQERERVEQEYVASAMHGDS
                     HDRYERLTSVSSSVDFDQRDNGFCSWLTAIFRIKDDEIRDKCGGDAVHYLSFQRHIIG
                     LLVVVGVLSVGIVLPVNFSGDLLENNAYSFGRTTIANLKSGNNLLWLHTSFAFLYLLL
                     TVYSMRRHTSKMRYKEDDLVKRTLFINGISKYAESEKIKKHFEEAYPNCTVLEARPCY
                     NVARLMFLDAERKKAERGKLYFTNLQSKENVPAMINPKPCGHLCCCVVRGCEQVEAIE
                     YYTKLEQRLKEDYRREKEKVNEKPLGMAFVTFHNETITAIILKDFNVCKCQGCTCRGE
                     PRASSCSEALHISNWTVTYAPDPQNIYWEHLSIRGFIWWLRCLVINVVLFILLFFLTT
                     PAIIITTMDKFNVTKPVEYLNNPIITQFFPTLLLWCFSALLPTIVYYSAFFEAHWTRS
                     GENRTTMHKCYTFLIFMVLLLPSLGLSSLDLFFRWLFDKKFLAEAAIRFECVFLPDNG
                     AFFVNYVIASAFIGNAMDLLRIPGLLMYMIRLCLARSAAERRNVKRHQAYEFQFGAAY
                     AWMMCVFTVVMTYSITCPIIVPFGLMYMLLKHLVDRYNLYYAYLPAKLDKKIHSGAVN
                     QVVAAPILCLFWLLFFSTMRTGFLAPTSMFTFVVLVITIVICLCHVCFGHFKYLSAHN
                     YKIEHTETDAVSSRSNGRPPTAGAVPKSAKYIAQVLQDSEGDGDGDGAPGSSGDEPPS
                     SSSQDEELLMPPDGLTDTDFQSCEDSLIENEIHQ"
     exon            524..603
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            604..642
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            643..733
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            734..771
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            772..914
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            915..966
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1075
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1146
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1227
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1351
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1485
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1623
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1777
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            1778..1884
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..1971
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            1972..2037
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            2038..2200
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..2306
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            2307..2474
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            2475..2590
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            2591..2671
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     exon            2672..3476
                     /gene="Tmem63b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3457..3462
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem63b"
                     /note="hexamer: AATAAA"
     polyA_site      3476
                     /gene="Tmem63b"
                     /note="major polyA site"
ORIGIN      
        1 gctccccctc ccccacgcgc tgccgcggcc gccgcaggag cccagagctc tagccgcccg
       61 gcgactcccc ctccccctcc ccagccccgc cccgcccgga gccggggctc ccgagtcgga
      121 gccgagtctg cgcctagggg tgagtacgcg gacccctccc cagcacccct tacttggctc
      181 ataggatctc accaccaccc caaggaagga aaaaaaaagc acccggactc agctgcgcgt
      241 cccctccctt ccacctcctg cgggacccag aggtgcccgg gccccacggg acccaacgga
      301 ggcacgcttt gtccccaaac ccgcaccgtc cctgccagat gaggatcatg cggcagtagc
      361 agccatgctg ccgttcttgc tggccacact gggcaccgcg gccctcaaca gcagcaaccc
      421 gaaggactac tgctacagtg cccggatccg cagcactgtc ctgcagggcc tgcccttcgg
      481 gggcgtcccc accgtgctgg ctctggactt catgtgcttt cttgccctgc tgttcttatt
      541 ctctattctc cggaaggtgg cctgggacta cgggcggctg gccttggtga cagatgcaga
      601 caggcttcgc cggcaggaga gggagcgagt ggaacaggaa tatgtggcat cagctatgca
      661 tggggacagt cacgaccggt atgagcgtct cacctccgtc tccagctctg tcgactttga
      721 ccaaagggac aatggcttct gttcctggct gacagccatc ttcaggataa aggacgatga
      781 gatccgggac aagtgtgggg gcgacgctgt gcactacctg tccttccaaa gacacatcat
      841 tgggttgctg gttgtcgtgg gcgtcctctc tgtaggcatt gtgctgcctg tcaacttctc
      901 aggggacctg ctagagaaca atgcctacag ctttgggaga accaccattg ccaacttgaa
      961 atcagggaat aacctgctct ggctacatac ctccttcgcc ttcctctacc tgctgctcac
     1021 cgtctacagc atgaggagac acacctctaa gatgcgctac aaggaagatg atctggtcaa
     1081 gcggactctt ttcatcaatg gaatctccaa atatgcagag tcggaaaaaa tcaagaaaca
     1141 tttcgaggag gcctacccca actgcacagt tctggaagcc cggccctgtt acaacgtggc
     1201 tcgacttatg ttccttgatg ctgagaggaa gaaggctgag cggggaaaac tctactttac
     1261 gaacctgcaa agcaaggaaa atgtacccgc catgatcaac cccaaaccgt gtggtcacct
     1321 ctgctgctgt gtggttcgag gctgcgagca ggtggaggcc attgagtact acacaaagct
     1381 ggagcagagg ctgaaggaag actacaggcg ggagaaggag aaagtgaacg agaaacctct
     1441 gggcatggcc tttgtcacct tccacaatga gaccatcaca gccatcatcc taaaggactt
     1501 caacgtgtgc aaatgccagg gctgtacctg ccgcggggag ccgagagcct cctcctgcag
     1561 cgaagcactg catatctcca actggaccgt gacctacgcc cctgaccctc agaacatcta
     1621 ttgggaacac ctctccatcc gaggcttcat ctggtggctg cgctgcctgg tcatcaatgt
     1681 ggtcctcttc attctgctct tcttcctcac caccccggcc atcatcatca ccaccatgga
     1741 caagttcaac gtcaccaagc ctgtggagta cctcaacaac ccgatcatca cccagttctt
     1801 ccctacgcta ctgctgtggt gcttctcggc cctgctcccc accattgtct actactccgc
     1861 cttcttcgaa gcacactgga cgcgctccgg ggagaacagg acgaccatgc acaagtgcta
     1921 cacctttctc atcttcatgg tgctgcttct gccctcgctg gggctgagca gcctagacct
     1981 cttcttccgc tggctctttg acaagaaatt cctggctgaa gcagctattc ggtttgagtg
     2041 tgtgttcctg cccgacaacg gcgctttctt cgtgaactac gtcattgcct cagcctttat
     2101 cggtaacgcc atggacctgc tgcgcatccc aggcctactc atgtacatga tccggctctg
     2161 cctggcgcgc tccgccgctg aaagacgcaa cgtgaagcgg catcaggcct acgagttcca
     2221 gtttggcgca gcctacgctt ggatgatgtg cgtcttcacg gtggtcatga cctacagtat
     2281 cacctgcccc atcatcgtgc ccttcgggct catgtacatg ctgctgaagc acctggtaga
     2341 caggtacaat ctctactacg cctacttgcc ggccaaactg gacaagaaga tccactcggg
     2401 ggctgtgaac caggtggtgg ctgcgcccat cctctgcctc ttctggttgc tcttcttctc
     2461 caccatgcgc acgggtttcc tagcccccac gtctatgttc accttcgttg ttctggtaat
     2521 caccatcgtc atctgtctct gccacgtctg ctttggacac ttcaaatacc tcagtgccca
     2581 caactacaag attgaacaca cagagacaga tgccgtgagc tccagaagta atggacggcc
     2641 ccccactgct ggcgctgtcc ccaaatctgc gaaatacatc gctcaggtgc tgcaggactc
     2701 agagggggac ggggacggag atggggctcc tgggagctca ggcgatgagc ccccatcgtc
     2761 ctcctcccaa gacgaggagt tgctgatgcc tcctgatggc ctcacggaca cagacttcca
     2821 gtcatgcgag gacagcctca tagagaatga gattcaccag taaggggagg gaggggccct
     2881 ggaggccacg ccctgcccct ccccccaccc tcacggacac taaaaaaaaa aaacgctaat
     2941 aatttattag aactaaagcc ccctcctcct accccatccc ctgctttcat taaggtattt
     3001 aaacctgggg atttcaccac tctctcccac catggaggga gagagggagc ccccccaacc
     3061 tcagtgagga cagccccgag caggccccgg ggcaaagagg ggtgtggagg gaataccccc
     3121 agatcagcct caccacccag tagtgtggcc aggaaagggt taatgagagc caaggtgggt
     3181 acctggaacc atggctggag gcctggggaa agcaagatca ggagttgtca cccagtctct
     3241 ttcctcccct tcactgcccc tggatctggg cctccaggga cttggaacct gcagccctct
     3301 gggatgcagg agaagggagg ggtgcacagt gtgggggaag agtcaggccc gagccagggg
     3361 ttgaccccgg ggggaggggg gcatggctga cactggaaaa tggggttttt gcactgggtt
     3421 tgttggtttt ttgtttttgt ttttgttttc atttaaaata aaaacaaaag ctgtga
//
