LOCUS       NM_001417554            4569 bp    mRNA    linear   ROD 05-DEC-2024
DEFINITION  Mus musculus transmembrane protein 63a (Tmem63a), transcript
            variant 4, mRNA.
ACCESSION   NM_001417554 XM_036163150
VERSION     NM_001417554.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Chen,G.L., Li,J.Y., Chen,X., Liu,J.W., Zhang,Q., Liu,J.Y., Wen,J.,
            Wang,N., Lei,M., Wei,J.P., Yi,L., Li,J.J., Ling,Y.P., Yi,H.Q.,
            Hu,Z., Duan,J., Zhang,J. and Zeng,B.
  TITLE     Mechanosensitive channels TMEM63A and TMEM63B mediate lung
            inflation-induced surfactant secretion
  JOURNAL   J Clin Invest 134 (5), e174508 (2024)
   PUBMED   38127458
  REMARK    GeneRIF: Mechanosensitive channels TMEM63A and TMEM63B mediate lung
            inflation-induced surfactant secretion.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Li,K., Guo,Y., Wang,Y., Zhu,R., Chen,W., Cheng,T., Zhang,X.,
            Jia,Y., Liu,T., Zhang,W., Jan,L.Y. and Jan,Y.N.
  TITLE     Drosophila TMEM63 and mouse TMEM63A are lysosomal mechanosensory
            ion channels
  JOURNAL   Nat Cell Biol 26 (3), 393-403 (2024)
   PUBMED   38388853
  REMARK    GeneRIF: Drosophila TMEM63 and mouse TMEM63A are lysosomal
            mechanosensory ion channels.
REFERENCE   3  (bases 1 to 4569)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 4569)
  AUTHORS   Zheng,W., Rawson,S., Shen,Z., Tamilselvan,E., Smith,H.E.,
            Halford,J., Shen,C., Murthy,S.E., Ulbrich,M.H., Sotomayor,M.,
            Fu,T.M. and Holt,J.R.
  TITLE     TMEM63 proteins function as monomeric high-threshold
            mechanosensitive ion channels
  JOURNAL   Neuron 111 (20), 3195-3210 (2023)
   PUBMED   37543036
REFERENCE   5  (bases 1 to 4569)
  AUTHORS   Pu,S., Wu,Y., Tong,F., Du,W.J., Liu,S., Yang,H., Zhang,C., Zhou,B.,
            Chen,Z., Zhou,X., Han,Q. and Du,D.
  TITLE     Mechanosensitive Ion Channel TMEM63A Gangs Up with Local
            Macrophages to Modulate Chronic Post-amputation Pain
  JOURNAL   Neurosci Bull 39 (2), 177-193 (2023)
   PUBMED   35821338
  REMARK    GeneRIF: Mechanosensitive Ion Channel TMEM63A Gangs Up with Local
            Macrophages to Modulate Chronic Post-amputation Pain.
REFERENCE   6  (bases 1 to 4569)
  AUTHORS   Murthy,S.E., Dubin,A.E., Whitwam,T., Jojoa-Cruz,S., Cahalan,S.M.,
            Mousavi,S.A.R., Ward,A.B. and Patapoutian,A.
  TITLE     OSCA/TMEM63 are an Evolutionarily Conserved Family of Mechanically
            Activated Ion Channels
  JOURNAL   Elife 7, e41844 (2018)
   PUBMED   30382938
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4569)
  AUTHORS   Zhao,X., Yan,X., Liu,Y., Zhang,P. and Ni,X.
  TITLE     Co-expression of mouse TMEM63A, TMEM63B and TMEM63C confers
            hyperosmolarity activated ion currents in HEK293 cells
  JOURNAL   Cell Biochem Funct 34 (4), 238-241 (2016)
   PUBMED   27045885
  REMARK    GeneRIF: TMEM63 proteins may serve as an osmolarity sensitive ion
            channel for the osmoreception.
REFERENCE   8  (bases 1 to 4569)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC117673.18.
            
            On Mar 6, 2023 this sequence version replaced XM_036163150.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC014795.1, SRR5286960.69919.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1371              AC117673.18        93134-94504
            1372-1571           AC117673.18        96974-97173
            1572-1651           AC117673.18        99221-99300
            1652-1715           AC117673.18        102540-102603
            1716-1753           AC117673.18        103389-103426
            1754-1896           AC117673.18        105216-105358
            1897-1948           AC117673.18        106401-106452
            1949-2057           AC117673.18        106828-106936
            2058-2128           AC117673.18        108385-108455
            2129-2209           AC117673.18        110834-110914
            2210-2333           AC117673.18        111143-111266
            2334-2467           AC117673.18        111456-111589
            2468-2605           AC117673.18        112755-112892
            2606-2759           AC117673.18        113407-113560
            2760-2866           AC117673.18        115948-116054
            2867-2953           AC117673.18        116152-116238
            2954-3016           AC117673.18        116876-116938
            3017-3179           AC117673.18        119153-119315
            3180-3285           AC117673.18        120588-120693
            3286-3453           AC117673.18        122375-122542
            3454-3569           AC117673.18        123124-123239
            3570-3632           AC117673.18        123548-123610
            3633-4569           AC117673.18        124577-125513
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 84.46 cM"
     gene            1..4569
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /note="transmembrane protein 63a"
                     /db_xref="GeneID:208795"
                     /db_xref="MGI:MGI:2384789"
     exon            1..1371
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1571
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     CDS             1386..3800
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /note="isoform 1 is encoded by transcript variant 4;
                     CSC1-like protein 1"
                     /codon_start=1
                     /product="CSC1-like protein 1 isoform 1"
                     /protein_id="NP_001404483.1"
                     /db_xref="GeneID:208795"
                     /db_xref="MGI:MGI:2384789"
                     /translation="MTSSPFLDPWPSKAVFVRERLGLGERPNDSYCYNSAKNSTVLQG
                     VTFGGIPTVLLLDVSCFLFLILVFSIIRRRFWDYGRIALVSEAGSEARFQRLSSSSSG
                     QQDFENELGCCPWLTAIFRLHDDQILEWCGEDAIHYLSFQRHIIFLLVVISFLSLCVI
                     LPVNLSGDLLGKDPYSFGRTTIANLQTDNDLLWLHTVFSVIYLFLTVGFMWHHTRSIR
                     YKEESLVRQTLFITGLPREARKETVESHFRDAYPTCEVVDVQLCYSVAKLIYLCKERK
                     KTEKSLTYYTNLQAKTGRRTLINPKPCGQFCCCEVQGCEREDAISYYTRMNDSLLERI
                     TAEESRVQDQPLGMAFVTFREKSMATYILKDFNACKCQGLRCKGEPQPSSYSRELCVS
                     KWTVTFASYPEDICWKNLSIQGVRWWLQWLGINFSLFVVLFFLTTPSIIMSTMDKFNV
                     TKPIHALNNPVISQFFPTLLLWSFSALLPSIVYYSTLLESHWTRSGENRIMVSKVYIF
                     LIFMVLILPSLGLTSLDFFFRWLFDKTSSETSIRLECVFLPDQGAFFVNYVIASAFIG
                     SGMELLRLPGLILYTFRMIMAKTAADRRNVKQNQAFEYEFGAMYAWMLCVFTVIMAYS
                     ITCPIIVPFGLIYILLKHMVDRHNLYFAYLPAKLEKRIHFAAVNQALAAPILCLFWLF
                     FFSFLRLGLTAPATLFTFLVVLLTILACLLYTCFGCFKHLSPWNYKTEESASDKGSEA
                     EAHVPPPFTPYVPRILNGLASERTALSPQQQQTYGAIRNISGTLPGQPVAQDPSGTAA
                     YAYQES"
     exon            1572..1651
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..1715
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1753
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..1896
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..1948
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2057
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            2058..2128
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2209
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            2210..2333
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            2334..2467
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            2468..2605
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            2606..2759
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            2760..2866
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            2867..2953
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            2954..3016
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            3017..3179
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            3180..3285
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            3286..3453
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            3454..3569
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            3570..3632
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
     exon            3633..4569
                     /gene="Tmem63a"
                     /gene_synonym="Tmem64a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctgggactt cctgacagct cttggctgcg tggcggatcc ttccctccac ctcctcggcg
       61 ggctttggag tttgtttgtg gagtcggatc tccccgccta acccgaaggg cagcgtcgga
      121 gccctctttt ctttaattcc ttacgaggct agacccggct tgggcgggct tcgtactgtc
      181 cgggtgacca ctccctcctg cccgggtagc accgggtgga ggaaaactgg ggacttggga
      241 aagggcggga gggggaggga agagctgaga cggcaaagaa accacatccg acaacaactt
      301 acccagacct gataagcctg gctacctgac acctcgggtg aggtggagtt gctgtctttc
      361 tgtcccaaac aggagccaac agcttgtagg gacttttgca aaaggcctgg gaacatattt
      421 tctctcctga agctttggct gggtcccaga gcctgccctg ttgagagttt ggggtgccaa
      481 tgatatggtg gagtgctgag cgtttgcgtg tacgaatatt cactggatgt gggatggtgg
      541 ctattgctaa gtgtgtacct aggcagggat cctgagacca cagtgtctaa ttgctgtttt
      601 taaggccccc tcgctcctcg tagtgatgcg gttggaatac agccttttgc cttgcacatt
      661 ctaggcgtgc acttggcttt gagcagcatt tccatccctt agggacttgt ttgatttctt
      721 ccctacacac acccagagcc ttctctctct ggcttggctg ccccttggtg ggtgtgctcc
      781 catctgccct ctcagagcgt gttcctttgg gaaggcaaga acggactggc tcattgaaag
      841 cctagagaat gtttctgtag cagcctctga ctctgctgct gctgggtggg cacaggttgc
      901 tccgactgga ggagagggtc tcggactgtg gcttctgatc cctctcactt gtcaagcctt
      961 tcttgtttgc tctgcggtat ttgatttccc tgattctagc ccacagggga tattgaggca
     1021 gagaagcagc ccttatgtct gcatgaggca gaataggcta cctggttgag gaggccctta
     1081 ggccttctgt tttgcttttg aggcagggtc ttactatgta gccacggatg gcctagaact
     1141 cacagagatc cacctgcctc tgcctcctga atgttggggt aaatgggtgg gtaccacaac
     1201 actctccctt ccttagttct taccaaactt tgtagattct cttgggaaac ctgtttggca
     1261 agagaaaaat gacttaggag tgtggcttgg ccactagggg aaggcagcca cagttaccct
     1321 cctggggtca aggcttctct gttcctccag gcctctgaaa gagcaacatt gggagagaag
     1381 gcacaatgac cagctccccg ttcctggatc cctggccgtc caaggctgtg tttgtccgtg
     1441 agcgcctggg tcttggcgaa cgtcccaatg actcctattg ctacaactca gccaaaaaca
     1501 gcactgtgct ccaaggggtc acctttggag gcatccccac tgtcctgctc ttagatgtca
     1561 gttgctttct gtttctaatc ctggtgtttt ccatcataag gagaagattc tgggattatg
     1621 gccgcatagc cctggtatca gaagcaggca gtgaggccag gttccagaga ctgtcctctt
     1681 cctcctcagg gcaacaagac tttgaaaacg agctgggatg ctgtccttgg ctgacggcca
     1741 tcttccgcct gcatgacgac cagatcctgg agtggtgcgg agaggacgcc atccactacc
     1801 tgtcttttca gaggcacatc atcttcctgc tggtggtgat cagcttcttg tccctgtgtg
     1861 tcatcttgcc tgtcaacctc tcgggagact tgctgggtaa agacccttac agctttggga
     1921 ggacaacgat agctaacctg cagactgaca atgacctcct ctggctgcac actgtcttca
     1981 gcgtcatcta cctcttcctc acggtgggct tcatgtggca tcacacacgg tccatcagat
     2041 acaaagagga gagtctggtg agacaaacct tgttcatcac aggactcccc agagaagcca
     2101 gaaaggagac tgtggagagc cacttccgag atgcgtatcc cacgtgtgag gtggtggatg
     2161 tccagctgtg ctacagcgtg gctaagctga tctacctgtg caaagagaga aagaagaccg
     2221 agaagagcct tacctattac acaaacctgc aggcgaagac aggccgacgg accctcatca
     2281 accccaagcc ttgtggccag ttctgctgct gtgaagttca gggttgtgag cgggaagatg
     2341 ctatttctta ctacactcga atgaatgaca gcctgctgga gaggatcaca gccgaagaga
     2401 gccgggtcca ggaccagccc ctgggaatgg cctttgtcac cttccgtgag aagtccatgg
     2461 ctacctacat actgaaagac ttcaatgcct gcaagtgcca gggccttcgg tgcaaagggg
     2521 agccccagcc atcctcctat agcagagagc tctgtgtctc caagtggacg gtcacttttg
     2581 cctcctatcc tgaggacatc tgctggaaga acctctccat ccagggcgtt cgctggtggc
     2641 tccagtggct gggtatcaac ttctctctgt ttgtggtgct gtttttcctg accacgccct
     2701 ccatcattat gtctaccatg gacaagttca atgttaccaa acccatccac gcactgaata
     2761 accccgtcat cagccagttc ttccccacac tcctgctgtg gtccttctct gcgctgcttc
     2821 cgtccattgt ctactactcc acgctgctgg agtcccactg gacaaggtca ggggaaaacc
     2881 ggatcatggt gtcgaaggtc tacatatttt tgatcttcat ggtgctgatc ctgccctccc
     2941 tcggtctcac cagcttggat ttcttcttcc ggtggctctt tgacaaaact tcctcggaaa
     3001 cctcaatcag gctagagtgt gtcttcctgc cagaccaggg tgccttcttt gtgaactacg
     3061 tcatcgcctc agccttcatc ggcagtggca tggagctgtt gcgcctgccc ggcctcatcc
     3121 tctatacctt ccgcatgatc atggccaaga cggccgctga ccgcaggaat gttaagcaga
     3181 accaggcttt tgaatacgag tttggagcca tgtatgcgtg gatgctgtgt gtcttcaccg
     3241 tcatcatggc ttacagcatc acctgcccca ttatcgtgcc ctttggcctc atctacatcc
     3301 tgcttaagca catggtggac cggcataacc tctacttcgc ttacctcccg gctaaactgg
     3361 agaagaggat ccacttcgcc gccgtgaacc aggccctggc cgcccccatc ctctgcctct
     3421 tctggctctt ctttttctcc ttcctccgtc tgggtctgac ggccccggcc acgctcttca
     3481 ccttcctggt tgttctgctc accatcctgg cctgcctgct gtacacctgc tttggatgct
     3541 tcaagcacct cagcccatgg aactacaaga cagaagaatc agctagtgac aaaggaagcg
     3601 aagccgaggc ccacgtgccc ccgccattca caccctatgt gcctcggatt ctgaacggtt
     3661 tggcctcaga gaggacagcg ttatccccac agcagcagca gacctacggt gccatccgta
     3721 acatcagtgg gactcttcct gggcagcccg tggctcaaga tcctagtggc actgcggctt
     3781 atgcctacca ggaatcctga gagtggacaa gctggtctgg caggctggga gcatctagga
     3841 ataccgaggg acacaagggg ctagagcccc agacaggctc ttgagggttt tgctgcttaa
     3901 aacccccgta ttgtgagaag aggaaaccag cgggtcgtgg ggctctgggt aaccgggctg
     3961 agggtatcca tccggtgggg ctgctgtccc tgggagccat ctgtaaggtt tcttttctgg
     4021 tcccagtgtg gggaacattt acagctccct cttgtggtca tctgaggata tgcactttta
     4081 ttcccccata cactgctcag gatgctctct gtagtgagca tggtgtatgt ccctagtggt
     4141 ttgtggccaa gatcatgtgt tgggcttttg cctttaggga gatcatattt ggggacttct
     4201 ggttgatggg gagatgcgtc caggaaagac ttagtccctc tgagccctgg aacactgctt
     4261 agccttctgc aagctgatga atagcgcatc agttgggtct gagatggagg ccatgtgggg
     4321 ccctacagta ctgtcctgcc tgcagtccct tttcagggat ccatggatta gctgtaggga
     4381 ccggggaata ccttgttctt gttgatggca gcagggcagc aggctcatgc ccactggttt
     4441 gtagctgcct cctgctgtgt ttccgggttt ttgtatcatg ttgcctgaat ctgggctgtg
     4501 actgggtaac cccacagcag gtggggacat gagaaccatt aataaagtac ccactgggct
     4561 ggtggtgag
//
