LOCUS       NM_001419296            2411 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus transmembrane protein 50B (Tmem50b), transcript
            variant 3, mRNA.
ACCESSION   NM_001419296 XM_036160181
VERSION     NM_001419296.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2411)
  AUTHORS   Dietrich,J.E., Panavaite,L., Gunther,S., Wennekamp,S., Groner,A.C.,
            Pigge,A., Salvenmoser,S., Trono,D., Hufnagel,L. and Hiiragi,T.
  TITLE     Venus trap in the mouse embryo reveals distinct molecular dynamics
            underlying specification of first embryonic lineages
  JOURNAL   EMBO Rep 16 (8), 1005-1021 (2015)
   PUBMED   26142281
REFERENCE   4  (bases 1 to 2411)
  AUTHORS   Tamplin,O.J., Kinzel,D., Cox,B.J., Bell,C.E., Rossant,J. and
            Lickert,H.
  TITLE     Microarray analysis of Foxa2 mutant mouse embryos reveals novel
            gene expression and inductive roles for the gastrula organizer and
            its derivatives
  JOURNAL   BMC Genomics 9, 511 (2008)
   PUBMED   18973680
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2411)
  AUTHORS   Moldrich,R.X., Laine,J., Visel,A., Beart,P.M., Laffaire,J.,
            Rossier,J. and Potier,M.C.
  TITLE     Transmembrane protein 50b (C21orf4), a candidate for Down syndrome
            neurophenotypes, encodes an intracellular membrane protein
            expressed in the rodent brain
  JOURNAL   Neuroscience 154 (4), 1255-1266 (2008)
   PUBMED   18541381
  REMARK    GeneRIF: Tmem50b is a developmentally-regulated intracellular ER
            and Golgi apparatus membrane protein that may prove important for
            correct brain development through functions associated with
            precursor cells and glia.
REFERENCE   6  (bases 1 to 2411)
  AUTHORS   Lau,N.C., Seto,A.G., Kim,J., Kuramochi-Miyagawa,S., Nakano,T.,
            Bartel,D.P. and Kingston,R.E.
  TITLE     Characterization of the piRNA complex from rat testes
  JOURNAL   Science 313 (5785), 363-367 (2006)
   PUBMED   16778019
REFERENCE   7  (bases 1 to 2411)
  AUTHORS   Gitton,Y., Dahmane,N., Baik,S., Ruiz i Altaba,A., Neidhardt,L.,
            Scholze,M., Herrmann,B.G., Kahlem,P., Benkahla,A., Schrinner,S.,
            Yildirimman,R., Herwig,R., Lehrach,H. and Yaspo,M.L.
  CONSRTM   HSA21 expression map initiative
  TITLE     A gene expression map of human chromosome 21 orthologues in the
            mouse
  JOURNAL   Nature 420 (6915), 586-590 (2002)
   PUBMED   12466855
REFERENCE   8  (bases 1 to 2411)
  AUTHORS   Reymond,A., Marigo,V., Yaylaoglu,M.B., Leoni,A., Ucla,C.,
            Scamuffa,N., Caccioppoli,C., Dermitzakis,E.T., Lyle,R., Banfi,S.,
            Eichele,G., Antonarakis,S.E. and Ballabio,A.
  TITLE     Human chromosome 21 gene expression atlas in the mouse
  JOURNAL   Nature 420 (6915), 582-586 (2002)
   PUBMED   12466854
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159199.3.
            
            On Mar 30, 2023 this sequence version replaced XM_036160181.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13861859.6074268.1,
                                           SRR17784645.675664.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-199               AC159199.3         206782-206980       c
            200-282             AC159199.3         203946-204028       c
            283-422             AC159199.3         196511-196650       c
            423-535             AC159199.3         192845-192957       c
            536-603             AC159199.3         191264-191331       c
            604-696             AC159199.3         189771-189863       c
            697-754             AC159199.3         187543-187600       c
            755-2411            AC159199.3         184071-185727       c
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 53.11 cM"
     gene            1..2411
                     /gene="Tmem50b"
                     /gene_synonym="B230114J08Rik"
                     /note="transmembrane protein 50B"
                     /db_xref="GeneID:77975"
                     /db_xref="MGI:MGI:1925225"
     exon            1..199
                     /gene="Tmem50b"
                     /gene_synonym="B230114J08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            200..282
                     /gene="Tmem50b"
                     /gene_synonym="B230114J08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            283..422
                     /gene="Tmem50b"
                     /gene_synonym="B230114J08Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             324..800
                     /gene="Tmem50b"
                     /gene_synonym="B230114J08Rik"
                     /note="isoform 1 is encoded by transcript variant 3"
                     /codon_start=1
                     /product="transmembrane protein 50B isoform 1"
                     /protein_id="NP_001406225.1"
                     /db_xref="GeneID:77975"
                     /db_xref="MGI:MGI:1925225"
                     /translation="MAGFLDNFRWPECECIDWSERRNTVASVVAGILFFTGWWIMIDA
                     AVVYPKPEQLNHAFHTCGVFSTLAFFMINAVSNAQVRGDSYESGCLGRTGARVWLFIG
                     FMLMFGSLIASMWILFGAYVTQNIDVYPGLAVFFQNALIFFSTLIYKFGRTEELWA"
     exon            423..535
                     /gene="Tmem50b"
                     /gene_synonym="B230114J08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            536..603
                     /gene="Tmem50b"
                     /gene_synonym="B230114J08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            604..696
                     /gene="Tmem50b"
                     /gene_synonym="B230114J08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            697..754
                     /gene="Tmem50b"
                     /gene_synonym="B230114J08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            755..2411
                     /gene="Tmem50b"
                     /gene_synonym="B230114J08Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2382..2387
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem50b"
                     /gene_synonym="B230114J08Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2411
                     /gene="Tmem50b"
                     /gene_synonym="B230114J08Rik"
                     /note="major polyA site"
ORIGIN      
        1 gacgccgccg tgacccgcgc tcagccgagg caggccgctc cgccgcgcat ctcgtcctga
       61 gtctggggag gtccccaagg cgatcggtag gggatggagg tcctgtgggg caccgccgca
      121 gccgccaccg aggccccacg agcgtctggg gacgagcgac cctcccgttt gctcggagcc
      181 gttgccagga actgtccttc tctcctgttg cagtcttcat agtgctaagg gttacagacg
      241 gcaccttgtc tgctcgtatg cacgcgagtc atctgtttgc ctgaaccatt gtttaatgag
      301 aactccatgt gtacagagtg aagatggcag gcttcctgga taacttccgc tggccggagt
      361 gtgagtgtat tgactggagc gagcgcagga acacggtggc ctctgtggtc gcagggatat
      421 tgttttttac aggctggtgg ataatgattg acgcggcagt ggtgtaccct aagcctgagc
      481 agctgaacca cgctttccac acgtgcggtg tgttttccac gctggccttc ttcatgataa
      541 atgctgtatc caatgcccag gtgcgaggtg acagctatga aagcggctgc ttaggacgaa
      601 ctggtgctcg ggtctggctc ttcattgggt tcatgctgat gttcgggtca ctcatcgcct
      661 ccatgtggat cctttttggt gcatatgtta ctcaaaatat tgatgtttac cctggactag
      721 ctgtgttctt tcaaaatgct cttatatttt ttagcactct gatctacaaa tttggaagaa
      781 ctgaagagct ctgggcctga gatcaccgca caccacccct tcctcttgta ttccatggcg
      841 agctccgtta tctccagtat atgacgttac agattgccaa atggagcgtt gtgtgctcag
      901 ggcttcattt cttcatagtt ttgtgccctg agtttgtctt ggtttttcat cgactaggca
      961 agctaatatg ttgataatat atcactacgt atacatgacg ctgaagtcag gtttttactc
     1021 ccaagactaa gattggtatg gctcccgagc cagggttatg ctgtcttggt gtttagaggt
     1081 aaatactttg tcttggtatc tctgatgttt gaaaatgtgc aaaatacaca gctccgtgtc
     1141 cctagaggcc ctgagccatt aacgaaggca tttctgccag gtgtaattta ttctgactta
     1201 gcaatgagat ttttttttca aggaaaaaaa agtttgtgca tatgtattaa agagctggac
     1261 tttttatatg aagatttttt taaatgccca aaggactagt ttgaaagccc tctttgcaaa
     1321 gaattcctct aacttgactt ttgtttgaga agggataaca cagagataac actccatttt
     1381 gtaaatcaga cggcaggcag ctgagctctg tgcttcccgt ctcaccgagc agcttggatg
     1441 cccaggttgt tgttgtggat ggcctgcctc cgtccggcat taacacacag caagggaccc
     1501 tgcagcagcc gcagtttaga tgcatggcca agtggaataa ctagctaacg acctcttccc
     1561 gaagggcgct gtgacctaca aaaggccagc tgcttcactc agtttgttct gactccttct
     1621 gtgtagctct caggctctgg gcgcccagct gctgcagagt acaggtatcc tgtactgaaa
     1681 gttcgcactc cacaccagac cctccttccc atgtttgagc gctgcgcttg ccccaagtat
     1741 atttctgtgg gcctttccct tgagtcaaga ttttgtgttg cactttcaac ccgagaggat
     1801 ttttataaga caatttgctg taaaccagta gtaaggatgg aagcagtgca cctcccatgc
     1861 aggcctcact ttacttagcg gcctctctga gaggcccctc caagcacagc gcgtgaagcg
     1921 tctgctgatc gctgcgcttc atcctgatgc atcttgcttc attactttcc catcagtccc
     1981 tcaaaatgtc agctttgtct agctagtgaa ctagaaagct agccacatgt ttccattctt
     2041 agaattgatg caataaatac tccctctcac actcgctgca cagttaggtg tgctggggga
     2101 agtgacccgt acaaccttgc tctgcatcct ccactgtcca agaaacaagg agaggacaca
     2161 ctgtacacat tgtgataata cagaggtcta tgaagggcag ccctgacgac cgactgaaaa
     2221 tggcggtggc ttctctattc atgaattttg ttttaattgt gagcaaatct gttgatttcc
     2281 tctgttgttt cattaaatga gagatgaagt tagtatcctc ttatagatgt tatgagtcag
     2341 tagaattctt ggtaatcata taaaatgcac acggttctct taataaaggt ctcactgttg
     2401 ctcaagctcg a
//
