LOCUS       NM_001419209            1242 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus transmembrane protein 50A (Tmem50a), transcript
            variant 2, mRNA.
ACCESSION   NM_001419209 XM_006539181
VERSION     NM_001419209.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Georgas,K., Rumballe,B., Valerius,M.T., Chiu,H.S.,
            Thiagarajan,R.D., Lesieur,E., Aronow,B.J., Brunskill,E.W.,
            Combes,A.N., Tang,D., Taylor,D., Grimmond,S.M., Potter,S.S.,
            McMahon,A.P. and Little,M.H.
  TITLE     Analysis of early nephron patterning reveals a role for distal RV
            proliferation in fusion to the ureteric tip via a cap
            mesenchyme-derived connecting segment
  JOURNAL   Dev Biol 332 (2), 273-286 (2009)
   PUBMED   19501082
REFERENCE   3  (bases 1 to 1242)
  AUTHORS   Moldrich,R.X., Laine,J., Visel,A., Beart,P.M., Laffaire,J.,
            Rossier,J. and Potier,M.C.
  TITLE     Transmembrane protein 50b (C21orf4), a candidate for Down syndrome
            neurophenotypes, encodes an intracellular membrane protein
            expressed in the rodent brain
  JOURNAL   Neuroscience 154 (4), 1255-1266 (2008)
   PUBMED   18541381
REFERENCE   4  (bases 1 to 1242)
  AUTHORS   Kumada,M., Iwamoto,S., Kamesaki,T., Okuda,H. and Kajii,E.
  TITLE     Entire sequence of a mouse chromosomal segment containing the gene
            Rhced and a comparative analysis of the homologous human sequence
  JOURNAL   Gene 299 (1-2), 165-172 (2002)
   PUBMED   12459264
REFERENCE   5  (bases 1 to 1242)
  AUTHORS   Schuh,R., Vestweber,D., Riede,I., Ringwald,M., Rosenberg,U.B.,
            Jackle,H. and Kemler,R.
  TITLE     Molecular cloning of the mouse cell adhesion molecule uvomorulin:
            cDNA contains a B1-related sequence
  JOURNAL   Proc Natl Acad Sci U S A 83 (5), 1364-1368 (1986)
   PUBMED   2419906
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL611963.24.
            
            On Mar 29, 2023 this sequence version replaced XM_006539181.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13948564.1795673.1,
                                           SRR9219381.82859.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-108               AL611963.24        75453-75560         c
            109-279             AL611963.24        73655-73825         c
            280-392             AL611963.24        70177-70289         c
            393-460             AL611963.24        65908-65975         c
            461-553             AL611963.24        64214-64306         c
            554-614             AL611963.24        61264-61324         c
            615-1242            AL611963.24        58413-59040         c
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 67.14 cM"
     gene            1..1242
                     /gene="Tmem50a"
                     /gene_synonym="3200001F09Rik; CAM; Smp1"
                     /note="transmembrane protein 50A"
                     /db_xref="GeneID:71817"
                     /db_xref="MGI:MGI:1919067"
     exon            1..108
                     /gene="Tmem50a"
                     /gene_synonym="3200001F09Rik; CAM; Smp1"
                     /inference="alignment:Splign:2.1.0"
     exon            109..279
                     /gene="Tmem50a"
                     /gene_synonym="3200001F09Rik; CAM; Smp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             187..660
                     /gene="Tmem50a"
                     /gene_synonym="3200001F09Rik; CAM; Smp1"
                     /note="isoform a is encoded by transcript variant 2; small
                     membrane protein 1"
                     /codon_start=1
                     /product="transmembrane protein 50A isoform a"
                     /protein_id="NP_001406138.1"
                     /db_xref="GeneID:71817"
                     /db_xref="MGI:MGI:1919067"
                     /translation="MSGFLEGSRCSECMDWGEKRNTIASIAAGVLFFTGWWIIIDAAV
                     MYPRMDQFNHSYHTCGVIATIAFLMINAVSNGQVRGDSYSEGCLGQTGARIWLFIGFM
                     LAFGSLIASMWILFGGYVAKEKDVVYPGIAVFFQNAFIFFGGLVFKFGRTEDLWQ"
     exon            280..392
                     /gene="Tmem50a"
                     /gene_synonym="3200001F09Rik; CAM; Smp1"
                     /inference="alignment:Splign:2.1.0"
     exon            393..460
                     /gene="Tmem50a"
                     /gene_synonym="3200001F09Rik; CAM; Smp1"
                     /inference="alignment:Splign:2.1.0"
     exon            461..553
                     /gene="Tmem50a"
                     /gene_synonym="3200001F09Rik; CAM; Smp1"
                     /inference="alignment:Splign:2.1.0"
     exon            554..614
                     /gene="Tmem50a"
                     /gene_synonym="3200001F09Rik; CAM; Smp1"
                     /inference="alignment:Splign:2.1.0"
     exon            615..1242
                     /gene="Tmem50a"
                     /gene_synonym="3200001F09Rik; CAM; Smp1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1219..1224
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem50a"
                     /gene_synonym="3200001F09Rik; CAM; Smp1"
                     /note="hexamer: ATTAAA"
     polyA_site      1242
                     /gene="Tmem50a"
                     /gene_synonym="3200001F09Rik; CAM; Smp1"
                     /note="major polyA site"
ORIGIN      
        1 acatcgacat ccgggagtct gattgcctgt ggctgttttg ttttcttgca gaactggagt
       61 aaaccgaggc taccaaacgc gccgcgactc ccaactccgg acttggacaa acacagccag
      121 ctttgaaaca agaaaaccga aggaccttgt ttaactgtga tggttttggt ttagtgacct
      181 gaaaagatgt ctggatttct agaaggctcg aggtgctctg agtgcatgga ctggggggag
      241 aagcggaaca cgatcgcctc catcgctgct ggcgttctat ttttcacggg atggtggatc
      301 atcatagacg cggctgtcat gtaccccagg atggatcaat tcaaccattc ataccatacc
      361 tgtggcgtca tagcaaccat agccttcctc atgattaacg cagtatcgaa cgggcaagtg
      421 cgaggcgaca gctacagcga aggctgtctg ggccagacag gagctcgcat ctggctcttc
      481 atcggcttca tgttggcttt cggatccctg attgcctcca tgtggattct ctttggaggt
      541 tatgtcgcta aggaaaagga cgtagtgtat cctgggattg ctgtattttt ccagaatgcc
      601 ttcatctttt ttggagggct ggttttcaag tttggccgta cggaggactt gtggcagtga
      661 agacgtcgct tctgcaacac agccgccgtg catgggcttc tttttgcacc ctgctcaccc
      721 acccagtctt ttgtaatact gttttctaaa cttatttctg agtataatct cagcttgaaa
      781 ttgtataatg ctagaatcat gagagctcct atgtgtactg cagcagccca gtgtggcgtg
      841 agccccatca gcttttacca caggaaagga aaccgctgac gtgagtcgct tctgccagct
      901 tctatcatgg tctaaagagt tggtgatgca ggccaggcgt ggtggcacat acctttaatc
      961 ccagcactcg ggaggcggag gcaggcggat ttctgagttc gaggccagcc tggtctacaa
     1021 agtgagttcc aggacagcca gggctgcaga gaaaccctgt ctcgaaaaac caaaaaaaaa
     1081 aaaacaaaaa aacaaaagag ttgttgatgg agaaatggtg gagtctttgg ggtgaggatt
     1141 cgctctgggg tccaaagtgt ttattaacgc ggcctcttct atgtccggtg ttgaataatg
     1201 tgtaaacatt ttcttgttat taaaaactat ttagtggtca ga
//
