LOCUS       NM_028975               6247 bp    mRNA    linear   ROD 01-AUG-2024
DEFINITION  Mus musculus transmembrane protein 33 (Tmem33), transcript variant
            1, mRNA.
ACCESSION   NM_028975
VERSION     NM_028975.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Yang,J.C., Zhao,J., Chen,Y.H., Wang,R., Rong,Z., Wang,S.Y.,
            Wu,Y.M., Wang,H.N., Yang,L. and Liu,R.
  TITLE     miR-29a-5p rescues depressive-like behaviors in a CUMS-induced
            mouse model by facilitating microglia M2-polarization in the
            prefrontal cortex via TMEM33 suppression
  JOURNAL   J Affect Disord 360, 188-197 (2024)
   PUBMED   38821373
  REMARK    GeneRIF: miR-29a-5p rescues depressive-like behaviors in a
            CUMS-induced mouse model by facilitating microglia M2-polarization
            in the prefrontal cortex via TMEM33 suppression.
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6247)
  AUTHORS   Liu,F., Ma,M., Gao,A., Ma,F., Ma,G., Liu,P., Jia,C., Wang,Y.,
            Donahue,K., Zhang,S., Ong,I.M., Keles,S., Li,L. and Xu,W.
  TITLE     PKM2-TMEM33 axis regulates lipid homeostasis in cancer cells by
            controlling SCAP stability
  JOURNAL   EMBO J 40 (22), e108065 (2021)
   PUBMED   34487377
REFERENCE   4  (bases 1 to 6247)
  AUTHORS   Arhatte,M., Gunaratne,G.S., El Boustany,C., Kuo,I.Y., Moro,C.,
            Duprat,F., Plaisant,M., Duval,H., Li,D., Picard,N., Couvreux,A.,
            Duranton,C., Rubera,I., Pagnotta,S., Lacas-Gervais,S.,
            Ehrlich,B.E., Marchant,J.S., Savage,A.M., van Eeden,F.J.M.,
            Wilkinson,R.N., Demolombe,S., Honore,E. and Patel,A.
  TITLE     TMEM33 regulates intracellular calcium homeostasis in renal tubular
            epithelial cells
  JOURNAL   Nat Commun 10 (1), 2024 (2019)
   PUBMED   31048699
  REMARK    GeneRIF: Study shows that TMEM33 interacts with the ion channel
            polycystin-2 at the endoplasmic reticulum (ER) membrane, enhancing
            its opening over the whole physiological calcium range in ER
            liposomes fused to planar bilayers. Results identify a key role for
            TMEM33 in the regulation of intracellular calcium homeostasis of
            renal proximal convoluted tubule cells and establish a causal link
            between TMEM33 and acute kidney injury.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6247)
  AUTHORS   Suzuki,H., Fukunishi,Y., Kagawa,I., Saito,R., Oda,H., Endo,T.,
            Kondo,S., Bono,H., Okazaki,Y. and Hayashizaki,Y.
  TITLE     Protein-protein interaction panel using mouse full-length cDNAs
  JOURNAL   Genome Res 11 (10), 1758-1765 (2001)
   PUBMED   11591653
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK139978.1, AK012413.1, AK017281.1, AK165369.1 and AC102896.9.
            
            On Oct 19, 2013 this sequence version replaced NM_028975.3.
            
            Transcript Variant: This variant (1) differs in the 5' UTR compared
            to variant 3. Variants 1 and 3 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK032555.1, AK165369.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-87                AK139978.1         612-698             c
            88-132              AK012413.1         2-46
            133-202             AK017281.1         2-71
            203-2541            AK165369.1         5-2343
            2542-6247           AC102896.9         61530-65235
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 36.0 cM"
     gene            1..6247
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /note="transmembrane protein 33"
                     /db_xref="GeneID:67878"
                     /db_xref="MGI:MGI:1915128"
     exon            1..242
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /inference="alignment:Splign:2.1.0"
     exon            243..312
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /inference="alignment:Splign:2.1.0"
     CDS             268..1011
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="transmembrane protein 33 isoform 1"
                     /protein_id="NP_083251.2"
                     /db_xref="CCDS:CCDS19317.1"
                     /db_xref="GeneID:67878"
                     /db_xref="MGI:MGI:1915128"
                     /translation="MADTTPNGPQGAGAVQFMMTNKLDTAMWLSRLFTVYCSALFVLP
                     LLGLHEAASFYQRALLANALTSALRLHQRLPHFQLSRAFLAQALLEDSCHYLLYSLIF
                     VNSYPVTMSIFPVLLFSLLHAATYTKKVLDAKGSNSLPLLRSFLDKLSTNQQNILKFI
                     ACNEIFLMPATVFMLFSGQGSLLQPFIYYRFLTLRYSSRRNPYCRNLFNELRIVVEHI
                     IMKPSCPLFVRRLCLQSIAFISRLAPTVA"
     exon            313..407
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /inference="alignment:Splign:2.1.0"
     exon            408..595
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /inference="alignment:Splign:2.1.0"
     exon            596..663
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /inference="alignment:Splign:2.1.0"
     exon            664..797
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /inference="alignment:Splign:2.1.0"
     exon            798..881
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /inference="alignment:Splign:2.1.0"
     exon            882..6247
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1293..1298
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /note="hexamer: AATACA"
     polyA_site      1318
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /note="major polyA site"
     regulatory      2518..2523
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /note="hexamer: AATAAA"
     polyA_site      2544
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /note="major polyA site"
     regulatory      6157..6162
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /note="hexamer: ATTAAA"
     polyA_site      6180
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
     regulatory      6225..6230
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /note="hexamer: AATAAA"
     polyA_site      6247
                     /gene="Tmem33"
                     /gene_synonym="1110006G02Rik; 1600019D15Rik;
                     2410089A21Rik; 2700052H22Rik; 5430406L04Rik; db83"
                     /note="major polyA site"
ORIGIN      
        1 gctcgtaggg aaggtggaac caaaacaagg agaaaaactg catttcccgt catgcctccg
       61 gaaaccacgc ccggtgactc tcaacgtccc gcctgactca cgtggaaacg aaaggatcac
      121 ttccggtttt gcaaccccgg gaaccggcgg cgtcgatcgg ccctttaagg cctttctccg
      181 aagctccgcc ttcgcttggg tctttctaga aaggcagctt cggtctggac cgagcggggc
      241 agcgtcccgg gctcccgagt gtctcccatg gcggatacga ccccgaacgg cccccaaggg
      301 gcgggcgctg tgcaattcat gatgaccaat aaattggaca cagcaatgtg gctttctcgc
      361 ctgttcacag tttattgctc cgctctgttc gttctgcctc ttcttgggtt gcatgaagca
      421 gcgagctttt accagcgtgc tttgctggcc aatgctctga ccagcgctct gaggctgcat
      481 cagagattac ctcacttcca gttgagcaga gcgttcctgg ctcaggcctt gttagaggac
      541 agctgccact acctgctgta ttcactcatc ttcgtcaact cctaccctgt cacaatgagt
      601 atttttccag tcttgttatt ttctttgctc catgctgcca catacactaa gaaggtcctg
      661 gacgcaaagg gttcaaatag tttacctctg ttgagatcat tcttggataa attaagtact
      721 aaccaacaaa atattctgaa attcatcgct tgtaatgaaa tattcttgat gcctgctaca
      781 gtttttatgc tctttagtgg ccaaggaagt ttgctccagc cttttatata ttatagattt
      841 cttactcttc gatattcctc tagaagaaat ccatattgtc ggaacttgtt taatgaactg
      901 aggattgtgg ttgaacacat tattatgaag ccctcctgcc cactgtttgt gagaagacta
      961 tgtctccaga gcatcgcctt tatcagcaga ctggcaccaa cagttgcgta gtttaacatc
     1021 tagttaagct gtgtttagaa gattaacatt attagctggt aattctgata ggggtcccaa
     1081 attacaggtc taacactgac ctcagtccaa tttacatgat ttacataaat gcatctcagt
     1141 gcaaaaataa taattttcct ggcatgttaa atcaagcctt taacactttc tgagaaaatt
     1201 ttactgtaca ttactgtttg tggttatgta agtttgtatt tagtgataaa tgttactaaa
     1261 ttgttttaga tgctgctgtg cctacatcat gaaatacatt tatttctttt aaaaagaatt
     1321 ctgaaatttg ttttacccta actattaacc cacgttatgt ctgttgtgag ttatggtact
     1381 agatgagttg agtatgtttg gagacagtgg ttttccattc tgtaagttgg gttgagattt
     1441 tagcagaagc acacctcctg cagtttcagt tcacacgcta ctatgctgct ttctagggca
     1501 ggtgtgttta gcctgcacca taacagagaa gtctgtctgc gtcttatgac ctgtgaagct
     1561 gttgttccca tagtgcatct gtggctatga atgatgtttg aaaaatggat tgcctgtaca
     1621 cctattttga attagatctt tggggccatg taatcattta ctgtagaaga cagtagtgac
     1681 acagagacct actgactaac ttttataaat gtcaaccagt agattatcag tgttcctata
     1741 gtttgtagga ttacatagtt ccagaatctt gactttagtt ttcttggttt cttaggcttg
     1801 aaatttccta gccatgtgca gcagcagttc agatgccttt gaaaggatat gattgaagac
     1861 agcatgtgat acacttgtat gttctgaaat aatgaagaat attctgtaga tgcatgttta
     1921 cccagcaaat gtggttcaga gggtttaatg gaaagggcta tgggtaaggc aactcatggg
     1981 gaatgggcct ggggagtagt tgagggcagc gatgggtgtt tcagcctggt aacgcataga
     2041 tgagttgggg ggattggatg tataatgcat ctgtgattga ggctgtgtaa tatgttcaat
     2101 tcttttcact caattctatt cctttataaa aggcattctg atccttacta ctcactgttg
     2161 catgattgga agtacttaaa acactgcaca agtttaatat agagtaatgg aatgtggctt
     2221 ttttttttaa tacccagttc tatctgcatg taatttggat ttttcaaata cattcattag
     2281 taacttaaaa catcagtttt ctttttgttt aagttactaa tctatagaaa ggtgatagtc
     2341 ttggggtaga tttccagaaa atactacttg atagcttagc tttaaccagc tacttttata
     2401 tttttgtaat atttatccac caagttggag aagcaacctc ataatatcca gttgattttt
     2461 ttatgtgtgt gcctagcctc cttatcacta tgttttaaga atacaacttt gtagagcaat
     2521 aaagtgacta tcttgttatg taaacatact gatttctaat gatgttttaa aacattgtct
     2581 tgaatgacat caagttctgg cagctaaagg catatttatt ttgaaatgtg ttttctccta
     2641 cattttcccc cctaatcttg gaacatttta gatttataag aacttcaaaa atagtgctga
     2701 gagtgcctat gcacctctta cttgtctatt tttatattag cgtcttcttt taattggtag
     2761 gacatttatg caaagtaaaa tactaacttt ggatcagcta aagtacaaga tgtgtttgta
     2821 tttcaccaac tctttcattt gtgtcgtgtg tgcgctgtgt tggtacctgg attgcctttt
     2881 atgtttcaag gcctataagt gagtttgttc tttatggtat tggtacagaa actttcctta
     2941 gaagagctaa ttcatgctga ctttaacatg aatttttttc ttaaaattgt aatttcaaat
     3001 gcttagtgat ataattaaga ctctttaatg gcattgcaga atttcattat ttctgataac
     3061 ttgtgcatga gtgaaaatgt atatttgttc cttaagggaa ggtgtaatat aattctcaag
     3121 cccatgagaa ggcaaatttt ataatttgaa cattaataga tgttagtttg catgaagttg
     3181 cagttagaaa tattatactc tgtgtgaata tgtacaatca actgatctca aatctgaaat
     3241 tagtgtttcc atgtacacat gtcaaaagtg aggacatgac tagtgcatag agtggacgag
     3301 caggggaact ttaatactgc gtaaccaagc agtttaaagg aagttgattg atcaagcaga
     3361 atatctagtg ctttggaaac tggaatggtg ctggcgtgtt ttaaaccttg ttggacaagt
     3421 ggaagcttca gacaaagtct gttgttttct aacaagcatt caatttctac tgcatacttc
     3481 atctctcacc gagttggctg taatttaata gtaatccata gagcaatttc ttaagtataa
     3541 tttgtattta aatttccttg aaataaaatt tcactaaggg agatgctttt gaaagaaaaa
     3601 caagagagaa agctaatttt aaagattaag ttgagatctt tgtatatatc cctgacagag
     3661 aatgtcaagg caagctagta acaaacctga agttctggga agtgaatgat gctgttaaat
     3721 aagaatcgca tcgttctcag tcggtataga gatgtagaca gaagtttgaa ttaggtttgt
     3781 aagtttagcg aatatcctgg ataggatcac gaatgcatat ataataagac aaagcagaag
     3841 ccaggtatct aagcatttca gatggggaag tgagcacctg tgattgtagt ttaaaaaaca
     3901 aaacaaaaac actcaggtag tgtattggaa tttgtattga ctaactcacc cagggagaaa
     3961 ttagctgtgg acagttaggt ttagatcggt aagtgtcact gtttccttgg gtagtcccca
     4021 ttttcacaag ttgggttaga agattatgct gtgctactta aagtgcttag tagactataa
     4081 ccctaagtat cttggttcta tgaccccttt aattcaatag tggacagtta tttgctaagt
     4141 atttactatg tgtgctgtga gatggggatg gggattgaag ccagggcccc ttgttctact
     4201 actgagctat attcccagct tatgagtacc attttataac agtaaaacat tcaaaagaag
     4261 tgcgttgtca atacaagcca ggaaaacagg gcccagtctg gagtggcgtg tgtgttagtg
     4321 tctcttcttt tcattttcag ccagtcatcc aagtacataa attgttgagg ttattctttt
     4381 cccttttaca aatgttcaaa gaaatgataa cttttctaat atagagagat ggaattttaa
     4441 ttttttcatg attattttgt aggtacacta gatagaatcc gttaaattac aattgagatt
     4501 tattttttcc cctgtaattt gaaagcactt gagtgtttgg aaactactat atcagtaatg
     4561 ctacctaggt ttgaagatcg agtgtagttc ataaagacaa caaattttcg ctccaccatt
     4621 ggaattccct ggatcaggga agttcaacac tggcaatgtg ttggtgtagc tcaacaggca
     4681 ctacatagct ttggaagccc ggagttgttt ctcagcagct ccttaatgca catggtcttt
     4741 catagtttga ctgcaaagcc attcttggag ctggtgtcta tcaaagatat caaaggagct
     4801 gctgggaatt tgaaaggttt cctgtaatta gttaatcagt gtgaattttg tgatttttga
     4861 gatggcactt aggtggccca ttcagaatgg atggaatctc gggtaaaggc ttctctctgt
     4921 ggccttctct ttgtgatgcg catgccatgt acccagccca cactgagtgt gagtcagtag
     4981 tgacagacag catgcagtaa gtattctctg tatctcaagt ggctctttct tagaaaacca
     5041 taggaaaaag attattgata gctgtgtttg ctagagtctt ttaacgacgc tattggctat
     5101 tgtaggagaa agaagggcac aagagtcata ccctaccttt tccttccttc cctccttcct
     5161 tccttccatc catccatcca tccatccatc cccctgagac ggggtttctc tttgtagacc
     5221 tttgaaccca gggatcctcc tgtctctgcc tcccatcttt cctagttttt taaaggtaca
     5281 ttaaaaaaaa taggctcagc attttttaaa ctaacgttgt tcataacatt taattccaaa
     5341 cgtttactat tttttgttac tttaaggaca attccttttg gcactagttt ttgtctacaa
     5401 gtatctgtaa gttgagacca aaagagcaaa ctcttagcca ggcaacactt acattgtgta
     5461 tatattggaa caaatataca aggcttgcag agattagcaa tactcatagg aactgtttga
     5521 tttataactg catcgcttct ggtgcttgat acaatgggat ctattgagct aaaaaaaatt
     5581 gtatatacag tacgtcagca cttcttacgt ctcttgaatc catttttaat atattgtata
     5641 ggttggggag ttatatatac tcttcaagcc attattatcc tggctataaa tttataaaat
     5701 aaattgaaaa atttatcact ctcctgtatt caagaccaaa gcacataatg ctaatgtagg
     5761 gctcagaggg gaaaatctag ttctcctgca tatttgagaa aacgtggagt cctttcaaga
     5821 aaatgtaata aacataataa tcgtagcctg ctgacactaa ggaaaaaggc ctcattcgct
     5881 ctttctttta tgcagcaatt tactggtccc tgctgatctt caaattggat cactgtagta
     5941 aattatccac gctggtactg gtggaagtaa gccctaggat ccatatttgt tttgtgttct
     6001 gcttaaatca gcaagaatga taaattcgat ggtgtgaaat tggaagtatc aagggctttc
     6061 tttggtgact gcacaaagtc atgtctccac ctggaattta ttatgacctt ttttcactgt
     6121 atgtacttca tatgtctaat atttatttca aaacaaatta aattgttctt ccttcatcta
     6181 taatgtttta tctaacattt tattgatgta aagtcaaatt gtgtaataaa agtcttcctg
     6241 gatttaa
//
