LOCUS       NM_133718               3680 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus transmembrane protein 30A (Tmem30a), mRNA.
ACCESSION   NM_133718
VERSION     NM_133718.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Chen,S., Song,X., Xiao,Q., Wang,L., Zhu,X., Zou,Y. and Li,G.
  TITLE     Knockdown of TMEM30A in renal tubular epithelial cells leads to
            reduced glucose absorption
  JOURNAL   BMC Nephrol 24 (1), 250 (2023)
   PUBMED   37612668
  REMARK    GeneRIF: Knockdown of TMEM30A in renal tubular epithelial cells
            leads to reduced glucose absorption.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3680)
  AUTHORS   Xing,Y., Peng,K., Yi,Q., Yu,D., Shi,H., Yang,G. and Yin,S.
  TITLE     TMEM30A is essential for hair cell polarity maintenance in
            postnatal mouse cochlea
  JOURNAL   Cell Mol Biol Lett 28 (1), 23 (2023)
   PUBMED   36959542
  REMARK    GeneRIF: TMEM30A is essential for hair cell polarity maintenance in
            postnatal mouse cochlea.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3680)
  AUTHORS   Li,T., Yu,D., Oak,H.C., Zhu,B., Wang,L., Jiang,X., Molday,R.S.,
            Kriegstein,A. and Piao,X.
  TITLE     Phospholipid-flippase chaperone CDC50A is required for synapse
            maintenance by regulating phosphatidylserine exposure
  JOURNAL   EMBO J 40 (21), e107915 (2021)
   PUBMED   34585770
  REMARK    GeneRIF: Phospholipid-flippase chaperone CDC50A is required for
            synapse maintenance by regulating phosphatidylserine exposure.
REFERENCE   5  (bases 1 to 3680)
  AUTHORS   Liu,W., Peng,L., Tian,W., Li,Y., Zhang,P., Sun,K., Yang,Y., Li,X.,
            Li,G. and Zhu,X.
  TITLE     Loss of phosphatidylserine flippase beta-subunit Tmem30a in
            podocytes leads to albuminuria and glomerulosclerosis
  JOURNAL   Dis Model Mech 14 (6) (2021)
   PUBMED   34080006
REFERENCE   6  (bases 1 to 3680)
  AUTHORS   Segawa,K., Kurata,S., Yanagihashi,Y., Brummelkamp,T.R., Matsuda,F.
            and Nagata,S.
  TITLE     Caspase-mediated cleavage of phospholipid flippase for apoptotic
            phosphatidylserine exposure
  JOURNAL   Science 344 (6188), 1164-1168 (2014)
   PUBMED   24904167
REFERENCE   7  (bases 1 to 3680)
  AUTHORS   Kato,U., Inadome,H., Yamamoto,M., Emoto,K., Kobayashi,T. and
            Umeda,M.
  TITLE     Role for phospholipid flippase complex of ATP8A1 and CDC50A
            proteins in cell migration
  JOURNAL   J Biol Chem 288 (7), 4922-4934 (2013)
   PUBMED   23269685
REFERENCE   8  (bases 1 to 3680)
  AUTHORS   Xu,Q., Yang,G.Y., Liu,N., Xu,P., Chen,Y.L., Zhou,Z., Luo,Z.G. and
            Ding,X.
  TITLE     P4-ATPase ATP8A2 acts in synergy with CDC50A to enhance neurite
            outgrowth
  JOURNAL   FEBS Lett 586 (13), 1803-1812 (2012)
   PUBMED   22641037
REFERENCE   9  (bases 1 to 3680)
  AUTHORS   Folmer,D.E., Mok,K.S., de Wee,S.W., Duijst,S., Hiralall,J.K.,
            Seppen,J., Oude Elferink,R.P. and Paulusma,C.C.
  TITLE     Cellular localization and biochemical analysis of mammalian CDC50A,
            a glycosylated beta-subunit for P4 ATPases
  JOURNAL   J Histochem Cytochem 60 (3), 205-218 (2012)
   PUBMED   22253360
  REMARK    GeneRIF: CDC50A is present as a fully glycosylated protein in vivo,
            which presumes interaction with distinct P4 ATPases.
REFERENCE   10 (bases 1 to 3680)
  AUTHORS   Ko,M.S., Threat,T.A., Wang,X., Horton,J.H., Cui,Y., Wang,X.,
            Pryor,E., Paris,J., Wells-Smith,J., Kitchen,J.R., Rowe,L.B.,
            Eppig,J., Satoh,T., Brant,L., Fujiwara,H., Yotsumoto,S. and
            Nakashima,H.
  TITLE     Genome-wide mapping of unselected transcripts from extraembryonic
            tissue of 7.5-day mouse embryos reveals enrichment in the t-complex
            and under-representation on the X chromosome
  JOURNAL   Hum Mol Genet 7 (12), 1967-1978 (1998)
   PUBMED   9811942
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK077065.1, AK159364.1 and BY470970.1.
            
            On Dec 4, 2008 this sequence version replaced NM_133718.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.129842.1, AK077065.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164133
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1595              AK077065.1         4-1598
            1596-1731           AK159364.1         1523-1658
            1732-3652           AK077065.1         1735-3655
            3653-3680           BY470970.1         469-496
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 43.82 cM"
     gene            1..3680
                     /gene="Tmem30a"
                     /gene_synonym="2010200I23Rik; Cdc50a; D9Wsu20e"
                     /note="transmembrane protein 30A"
                     /db_xref="GeneID:69981"
                     /db_xref="MGI:MGI:106402"
     exon            1..457
                     /gene="Tmem30a"
                     /gene_synonym="2010200I23Rik; Cdc50a; D9Wsu20e"
                     /inference="alignment:Splign:2.1.0"
     CDS             221..1315
                     /gene="Tmem30a"
                     /gene_synonym="2010200I23Rik; Cdc50a; D9Wsu20e"
                     /note="P4-ATPase flippase complex beta subunit TMEM30A"
                     /codon_start=1
                     /product="cell cycle control protein 50A"
                     /protein_id="NP_598479.1"
                     /db_xref="CCDS:CCDS23367.1"
                     /db_xref="GeneID:69981"
                     /db_xref="MGI:MGI:106402"
                     /translation="MAMNYSAKDEVDGGPAGPPGGAAKTRRPDNTAFKQQRLPAWQPI
                     LTAGTVLPTFFIIGLIFIPIGIGIFVTSNNIREIEIDYTGTEPSSPCNKCLSPNVTSC
                     ACTINFTLKQSFEGNVFMYYGLSNFYQNHRRYVKSRDDSQLNGDPSALLNPSKECEPY
                     RRNEDRPIAPCGAIANSMFNDTLELYLVANESDPKPIPIPLKKKGIAWWTDKNVKFRN
                     PPGKESLEEKFKDTIKPVNWHKAVYELDPEDESNNGFINEDFIVWMRTAALPTFRKLY
                     RLIERRDDLHPTLPAGQYFLNITYNYPVHSFDGRKRMILSTISWMGGKNPFLGIAYIT
                     IGSISFLLGVVLLVINHKYRNSSNTADITI"
     exon            458..565
                     /gene="Tmem30a"
                     /gene_synonym="2010200I23Rik; Cdc50a; D9Wsu20e"
                     /inference="alignment:Splign:2.1.0"
     exon            566..673
                     /gene="Tmem30a"
                     /gene_synonym="2010200I23Rik; Cdc50a; D9Wsu20e"
                     /inference="alignment:Splign:2.1.0"
     exon            674..761
                     /gene="Tmem30a"
                     /gene_synonym="2010200I23Rik; Cdc50a; D9Wsu20e"
                     /inference="alignment:Splign:2.1.0"
     exon            762..911
                     /gene="Tmem30a"
                     /gene_synonym="2010200I23Rik; Cdc50a; D9Wsu20e"
                     /inference="alignment:Splign:2.1.0"
     exon            912..1121
                     /gene="Tmem30a"
                     /gene_synonym="2010200I23Rik; Cdc50a; D9Wsu20e"
                     /inference="alignment:Splign:2.1.0"
     exon            1122..3660
                     /gene="Tmem30a"
                     /gene_synonym="2010200I23Rik; Cdc50a; D9Wsu20e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3640..3645
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem30a"
                     /gene_synonym="2010200I23Rik; Cdc50a; D9Wsu20e"
                     /note="hexamer: AATAAA"
     polyA_site      3660
                     /gene="Tmem30a"
                     /gene_synonym="2010200I23Rik; Cdc50a; D9Wsu20e"
                     /note="major polyA site"
ORIGIN      
        1 tttcctctct ctccctccct ggggcgtctc ctaaagacgc ccgcttccgg tcagcggccg
       61 gccgcagaag cgtgtcccgc ctcttccgcc tccccggtgg cggcgcgggt ggcggaggcg
      121 gtagcggcag cggtgactgc gcgtctcctg tcagggtcgg cggcacgcaa gtcccctggg
      181 cggcccagct ggacgtggcc gagccgaagc ccgggggccg atggcgatga actatagcgc
      241 gaaggatgag gtggacggcg ggcccgcggg tcctcccggg ggcgccgcca agacccggag
      301 gccggataac acggccttca aacagcaacg gctacccgcc tggcagccca tcctcacggc
      361 cggcacggtg ctgcccacct tcttcatcat cggcctcatc ttcatcccca tcggcatcgg
      421 catcttcgtc acctccaaca acatccgtga gatcgagatt gattacactg gaacagaacc
      481 ttccagcccc tgcaataaat gtttatctcc gaatgtgaca tcttgtgctt gtaccattaa
      541 cttcacactg aaacagtcat ttgagggcaa tgtgtttatg tattatggac tgtctaattt
      601 ctatcaaaat catcgtcgct acgtgaaatc tcgagatgat agccagttaa atggagaccc
      661 tagtgctttg cttaatccaa gtaaggaatg tgaaccttat agaagaaatg aagacagacc
      721 aattgcgcca tgtggcgcta ttgccaacag catgtttaat gatacgttag aattgtatct
      781 ggttgccaat gaatctgatc ccaagcctat tccaattcct ttgaagaaaa aaggtattgc
      841 ttggtggaca gataaaaatg tgaaattcag aaatccacct ggaaaagaga gcctcgaaga
      901 aaagtttaaa gatacaataa agccagtaaa ctggcataag gcagtatatg agctagaccc
      961 tgaagatgaa agtaataatg gattcataaa tgaagacttt atagtttgga tgcgtactgc
     1021 agcattacct acttttcgta agttgtatcg tctcatagag cggagagatg atttacaccc
     1081 aacattacca gctggacagt actttttgaa catcacatac aattaccctg tgcattcctt
     1141 tgatggacgg aaacggatga tcttgagcac tatttcatgg atgggaggaa agaatccatt
     1201 tttgggaatt gcttacatca ctattggatc catctctttc cttctgggag ttgtactgct
     1261 agtaattaat cataaatata gaaacagtag taacactgct gacatcacca tttaatttta
     1321 tattctgaaa ccaaatctac tgcatgtgca tcaaggccag tcctgttcaa cctagctttt
     1381 gaatgctgat gtctggttag tatgtcattt tgaagttggc acataacttt aaaaaaacaa
     1441 aacaaacaaa acagcctttg ttctttgctt cttacatatg gatgacttat gaaaatatat
     1501 gatggatata aaaattagcc atattgatta tatcaatatt gtaactgcta aaatgacatt
     1561 ctaatgtctg ctttttattg ggacaggcca tgtgatgcat agagcctctt tcctatgaaa
     1621 tgcgtctact gcttaaactg ttgatgctgt gttgataaca tattgacatg atgctgtata
     1681 tgtgtgccta ctgtgtgatg aaagggatta tgagatgtat gagtgtaatg acttgctaac
     1741 ctttgaaaat ttggttacag ttcagataga agaaagacta taaataaaac acttcatcat
     1801 tttcatgtgt cgtgtgtaaa ggcttaaagt ccctccttgt tgaggtggtt catatgttca
     1861 gttgctctat tatgattctc cgataatgac gttgacttca cactttagct tgtacaacat
     1921 agaaattaat atctaaagag gtcagtgggt cttggctaga attttaaatt tcttctcatt
     1981 tgagtaaaat gttgcattct gaagtcccat gctacctgaa gttgcatttg gagtcccaag
     2041 ctactggaat gtttatatgt gaccgtttcc caggaggctt acactgcaga aggaagaatg
     2101 aatctaggtg aggtgggcag ctgcttggca gtcctctcct gtgccccaac tgtaaaccag
     2161 atagaaatgt tcaggggagg atactttcat tattgtggtt tgtagtgtta agatgattgc
     2221 ttctgccttg gaaatacctc aagctgttct tatttaacag gtaagtgact gagtataata
     2281 ttccagaaaa atttgaaatc ctaatttctt ccatatttca ttaaattttt tgcatacagg
     2341 tctaacaaat atggatatgt atacacatcc tctttaatga aggtattatt ttggttactt
     2401 ttcctaagat ataccttaaa agatgttcta tacatttcct acttaaattc tgggggattt
     2461 ggagtatgta catgataaaa aagattataa tatatcgatt gaagttactt tattttctaa
     2521 ctagaattat tttaatagtc ctttattgaa taagtgctgt aatttgtttg ctatgagact
     2581 tattcctgat gtgaatgtaa attatttttc cacatgcatg aaaaaatgta tgtactaatc
     2641 agagttgtct ccactgcatt gaaattactt gttttgaact aaagtaactc atatttatgt
     2701 agtagaatgc ttatgttttc agactttgta atgatttcct ttggatgtat tttaaatcaa
     2761 tcggtctggg taacatatca gtttagatta atatgtgctt aaaagaagaa aaaaattcaa
     2821 tggttcatag tagaaatgtg ccacacttaa ataagctctg tatgacatga aattctgtta
     2881 aaacattgta attcatggtg acttttaact tataaaaata ctacttgcac gggttacttg
     2941 atttatggat atatgaaaac ttctcaggac gaaagttctt ctttctctag aactattctt
     3001 ctgtcggtca tgcagaatgc tgttattctg aaaagtgtcc ctgttgcata tgatggtcac
     3061 tttatttggg gggattcttc ataagatgtg agatgttgat gccagtcttt cccaagtaag
     3121 tgctcgtaaa aaaggactac taactagcct gcatctgtct ctaactggga ccaaggggtc
     3181 tgctgaagga aactgaagag ctctaacatt ttcacagctt ggagaagata gaatctttaa
     3241 aagtacaact gaagcttgat ctattttaca agtgcattga tggcccctgt ccttctctgg
     3301 ttcctgtcat ttgaaaccaa ctcctgttgt aaataggaag aatatgggac attcatattt
     3361 aagaaaattt gatgtcatta ggtgactaag tagaaggctt agaaaaatgt attcatttgc
     3421 aagtattttg gcacaagaaa ttttccaact gaatagtaag caaaagctaa gttgtttcat
     3481 tgaaatcata aggcagttta agataaactg gagaagataa ctgttctaat agaggataat
     3541 cgaattgatt gtcaagtgga tgttatttat tggatagtga cagagtttat ttgtaacctt
     3601 aattatatta aaagttattc tgttaggatg ttttgtatta ataaacgtga acaaaattaa
     3661 aaaaaaaaaa aaaaaaaaaa
//
