LOCUS       NM_194065                847 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus transmembrane protein 270 (Tmem270), transcript
            variant 1, mRNA.
ACCESSION   NM_194065
VERSION     NM_194065.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Lu,Y., Oura,S., Matsumura,T., Oji,A., Sakurai,N., Fujihara,Y.,
            Shimada,K., Miyata,H., Tobita,T., Noda,T., Castaneda,J.M.,
            Kiyozumi,D., Zhang,Q., Larasati,T., Young,S.A.M., Kodani,M.,
            Huddleston,C.A., Robertson,M.J., Coarfa,C., Isotani,A.,
            Aitken,R.J., Okabe,M., Matzuk,M.M., Garcia,T.X. and Ikawa,M.
  TITLE     CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched
            genes dispensable for male fertility in micedagger
  JOURNAL   Biol Reprod 101 (2), 501-511 (2019)
   PUBMED   31201419
REFERENCE   2  (bases 1 to 847)
  AUTHORS   DeSilva,U., Elnitski,L., Idol,J.R., Doyle,J.L., Gan,W.,
            Thomas,J.W., Schwartz,S., Dietrich,N.L., Beckstrom-Sternberg,S.M.,
            McDowell,J.C., Blakesley,R.W., Bouffard,G.G., Thomas,P.J.,
            Touchman,J.W., Miller,W. and Green,E.D.
  TITLE     Generation and comparative analysis of approximately 3.3 Mb of
            mouse genomic sequence orthologous to the region of human
            chromosome 7q11.23 implicated in Williams syndrome
  JOURNAL   Genome Res 12 (1), 3-15 (2002)
   PUBMED   11779826
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC114542.1 and AV209462.1.
            
            On Feb 8, 2013 this sequence version replaced NM_194065.2.
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the longer isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC114542.1, AY369079.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-846               BC114542.1         2-847
            847-847             AV209462.1         216-216
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 74.88 cM"
     gene            1..847
                     /gene="Tmem270"
                     /gene_synonym="1700111I05Rik; Wbscr28"
                     /note="transmembrane protein 270"
                     /db_xref="GeneID:76629"
                     /db_xref="MGI:MGI:1923879"
     exon            1..106
                     /gene="Tmem270"
                     /gene_synonym="1700111I05Rik; Wbscr28"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..829
                     /gene="Tmem270"
                     /gene_synonym="1700111I05Rik; Wbscr28"
                     /note="isoform a is encoded by transcript variant 1;
                     williams-Beuren syndrome chromosomal region 28 protein
                     homolog; Williams-Beuren syndrome critical region 28;
                     Williams-Beuren syndrome chromosome region 28"
                     /codon_start=1
                     /product="transmembrane protein 270 isoform a"
                     /protein_id="NP_919041.1"
                     /db_xref="CCDS:CCDS80423.1"
                     /db_xref="GeneID:76629"
                     /db_xref="MGI:MGI:1923879"
                     /translation="MEAAPRVRSGLLRILLRAGRLSALLIQNRTHLYRFLLLKMAIFQ
                     HWVLGLAQEARGSGSDQARQLPEVIITCALSLALRAGLTLLWVPMWLLLWGPRLAYRV
                     GLCCTRTVRLALGHLCVCEPLGLSPATFRDLFLSCLHSLMLVALLLLLLTWKLMQKAH
                     HFSLGWLPSQNSVLLEAPALLRRLYLWVEHRTTLTSWNLAYLVTWTTCLASHLLQAAF
                     EHTTQLAQAQEVKSQETSGPPPQFLIPESSTTESGPLPPQPETPGE"
     exon            107..544
                     /gene="Tmem270"
                     /gene_synonym="1700111I05Rik; Wbscr28"
                     /inference="alignment:Splign:2.1.0"
     exon            545..847
                     /gene="Tmem270"
                     /gene_synonym="1700111I05Rik; Wbscr28"
                     /inference="alignment:Splign:2.1.0"
     regulatory      825..830
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem270"
                     /gene_synonym="1700111I05Rik; Wbscr28"
                     /note="hexamer: AATAAA"
     polyA_site      846
                     /gene="Tmem270"
                     /gene_synonym="1700111I05Rik; Wbscr28"
                     /note="major polyA site"
ORIGIN      
        1 ctctcctggc tggaggaatg ggtggaggcc agacatggag gccgctcccc gggtcagatc
       61 tggccttctc agaattcttc tgcgggctgg gaggctctct gcactgctga tccagaaccg
      121 cactcacttg taccgctttc tgctcctgaa gatggccatc tttcagcact gggtgttggg
      181 gctggcccag gaagcccggg gttcaggcag tgaccaggcc cgccagcttc cggaagtcat
      241 cataacctgt gctctgagtt tggcccttcg agctggacta acattgctgt gggtccccat
      301 gtggctgctg ctctggggac ccaggctggc atacagagtc gggctgtgct gcactcgtac
      361 tgtgaggttg gccctggggc atctctgtgt ctgtgaacct ctcggtctgt ctccagccac
      421 cttcagggac ctgtttctat cctgcctgca cagcctgatg ctggtagctt tgctgctgct
      481 gctgctaacc tggaagctga tgcagaaagc ccatcacttt agtctgggct ggctgcccag
      541 tcagaattct gtgttgctgg aagccccggc cctgctgaga cgcctctacc tgtgggtgga
      601 acacaggacc acactcactt cctggaacct ggcctatctt gtcacttgga cgacctgcct
      661 tgcttcccac ctgcttcaag ctgcctttga acacacaacc caactggccc aggcccagga
      721 agtcaaatcc caggagacct cggggccccc gcctcagttc ttaattccag aatcctccac
      781 cactgagtct ggacccctcc caccccagcc tgaaacccct ggagaataaa tgtgtccccc
      841 acctgcc
//
