LOCUS       NM_001003949            2170 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus transmembrane protein 259 (Tmem259), transcript
            variant 2, mRNA.
ACCESSION   NM_001003949 XM_109575
VERSION     NM_001003949.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Jiang,L.L., Zhu,B., Zhao,Y., Li,X., Liu,T., Pina-Crespo,J.,
            Zhou,L., Xu,W., Rodriguez,M.J., Yu,H., Cleveland,D.W., Ravits,J.,
            Da Cruz,S., Long,T., Zhang,D., Huang,T.Y. and Xu,H.
  TITLE     Membralin deficiency dysregulates astrocytic glutamate homeostasis
            leading to ALS-like impairment
  JOURNAL   J Clin Invest 129 (8), 3103-3120 (2019)
   PUBMED   31112137
  REMARK    GeneRIF: reduced membralin and EAAT2 levels correlated with disease
            progression in spinal cord from SOD1-mutant mouse models, and
            reductions in membralin/EAAT2 were observed in human ALS spinal
            cord. Importantly, overexpression of membralin in SOD1G93A
            astrocytes decreased TNFR1 levels and increased EAAT2 expression,
            and improved motor neuron survival.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Gerber,H., Mosser,S., Boury-Jamot,B., Stumpe,M., Piersigilli,A.,
            Goepfert,C., Dengjel,J., Albrecht,U., Magara,F. and Fraering,P.C.
  TITLE     The APMAP interactome reveals new modulators of APP processing and
            beta-amyloid production that are altered in Alzheimer's disease
  JOURNAL   Acta Neuropathol Commun 7 (1), 13 (2019)
   PUBMED   30704515
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2170)
  AUTHORS   Zhu,B., Jiang,L., Huang,T., Zhao,Y., Liu,T., Zhong,Y., Li,X.,
            Campos,A., Pomeroy,K., Masliah,E., Zhang,D. and Xu,H.
  TITLE     ER-associated degradation regulates Alzheimer's amyloid pathology
            and memory function by modulating gamma-secretase activity
  JOURNAL   Nat Commun 8 (1), 1472 (2017)
   PUBMED   29133892
  REMARK    GeneRIF: The results identify membralin as an
            Endoplasmic-reticulum-associated degradation component and
            demonstrate a critical role for Endoplasmic-reticulum-associated
            degradation in Alzheimer's disease pathogenesis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2170)
  AUTHORS   Yang,B., Qu,M., Wang,R., Chatterton,J.E., Liu,X.B., Zhu,B.,
            Narisawa,S., Millan,J.L., Nakanishi,N., Swoboda,K., Lipton,S.A. and
            Zhang,D.
  TITLE     The critical role of membralin in postnatal motor neuron survival
            and disease
  JOURNAL   Elife 4, e06500 (2015)
   PUBMED   25977983
  REMARK    GeneRIF: Membralin plays a critical role in motor neuron survival,
            suggesting a novel mechanism for early-onset motor neuron disease.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2170)
  AUTHORS   Andersson,O. and von Euler,G.
  TITLE     Characterization and expression of the gene encoding membralin, an
            evolutionary conserved protein expressed in the central nervous
            system
  JOURNAL   Brain Res Gene Expr Patterns 1 (3-4), 205-212 (2002)
   PUBMED   12638133
  REMARK    GeneRIF: genomic organization and expression (membralin)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC151846.3, BQ572278.1, CX205815.1, BQ571954.1, CO044817.1 and
            BE947335.1.
            
            On Jan 13, 2018 this sequence version replaced NM_001003949.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY096036.1, SRR7345562.2519751.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AC151846.3         15739-15872
            135-846             BQ572278.1         2-713
            847-1146            CX205815.1         431-730
            1147-1751           BQ571954.1         76-680
            1752-2152           CO044817.1         20-420              c
            2153-2170           BE947335.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..2170
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /note="transmembrane protein 259"
                     /db_xref="GeneID:216157"
                     /db_xref="MGI:MGI:2177957"
     exon            1..351
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /inference="alignment:Splign:2.1.0"
     CDS             130..1854
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /note="isoform 2 is encoded by transcript variant 2;
                     membralin"
                     /codon_start=1
                     /product="membralin isoform 2"
                     /protein_id="NP_001003949.2"
                     /db_xref="CCDS:CCDS24002.1"
                     /db_xref="GeneID:216157"
                     /db_xref="MGI:MGI:2177957"
                     /translation="MSEHAAAPGPGPNGGGGGGAAPVRGPRGPNLNPNPLINVRDRLF
                     HALFFKMAVTYSRLFPPAFRRLFEFFVLLKALFVLFVLAYIHIVFSRSPINCLEHVRD
                     RWPREGVLRVEVRHNSSRAPVILQFCDGGLGGLELEPGGLELEEEELTVEMFTNSSIK
                     FELDIEPKVFKPQSGADALNDSQDFPFPETPAKVWPQDEYIVEYSLEYGFLRLSQATR
                     QRLSIPVMVVTLDPTRDQCFGDRFSRLLLDEFLGYDDILMSSVKGLAENEENKGFLRN
                     VVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMN
                     MAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILTVWLADQYDAICCHTNTSK
                     RHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIR
                     IQEMLLQTPPLGPGTPTALPDDLNNNSGSPATPDPSPPLALGPSSSPAPTGGASGPGS
                     LGAGASVSGSDLGWVAETAAIISDASFLSGLSASLLERRPTAPSTPDSSRPDPGVPLE
                     DAPAPAGS"
     exon            352..609
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /inference="alignment:Splign:2.1.0"
     exon            610..709
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /inference="alignment:Splign:2.1.0"
     exon            710..820
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /inference="alignment:Splign:2.1.0"
     exon            821..943
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1044
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1102
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..1186
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1319
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1419
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /inference="alignment:Splign:2.1.0"
     exon            1420..2153
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2133..2138
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /note="hexamer: AATAAA"
     polyA_site      2153
                     /gene="Tmem259"
                     /gene_synonym="AC087114.7gm2; mFLJ00277; ORF61; R32184_3"
                     /note="major polyA site"
ORIGIN      
        1 ggcgtcgcgg gccggacgga cgcgagcaaa ggggcaggcg gcggcggcgg ccgggggcgg
       61 cgggggggat ccggcggccg ggacgcggcg ggggtcagcc ggcggccgcc ggggtgacgc
      121 ggggcggtga tgtcggagca cgcggcagcc ccagggcccg ggcccaacgg cggcgggggt
      181 ggtggcgcgg cgcccgtgcg cggccctcgc ggccccaatc tcaaccccaa cccgctcatc
      241 aacgtgcgcg accggctctt ccacgctctc ttcttcaaga tggccgtcac ctactcacgc
      301 ctcttcccac ctgctttccg ccgcctcttt gaattcttcg ttctgctcaa ggccctgttt
      361 gtgcttttcg tcctcgccta catacacatc gtcttctccc ggtcccccat caactgctta
      421 gagcatgttc gagatcgatg gccgcgggag ggtgtcctgc gggtggaggt gcgccacaac
      481 tcgagccggg caccggtgat cctgcagttc tgtgatgggg gcctcggtgg cctggagctg
      541 gaacccgggg gcctggagct ggaggaggag gagctcacag tggagatgtt caccaacagc
      601 tccatcaagt ttgagctgga cattgagccc aaggtgttca agccacagag cggtgcagat
      661 gccctgaacg acagccagga cttccctttt cctgagacgc cagcaaaagt gtggccacag
      721 gatgagtaca ttgtggagta ctcactggaa tatggcttcc tgcggctatc ccaagccaca
      781 cgccagcgtc tgagcattcc tgtcatggtg gtcaccctag accccacgcg ggaccagtgc
      841 tttggggacc gcttcagccg cctattgctg gatgagttcc tgggctatga tgacatcctc
      901 atgtccagtg taaagggtct ggcagagaac gaggagaaca aaggcttctt gaggaatgtg
      961 gtctctgggg agcactaccg ctttgtcagc atgtggatgg cgcgcacatc ctacctggcg
     1021 gcctttgtca tcatggtcat ctttaccctc agcgtgtcca tgctgttgcg atactcgcac
     1081 caccagatct tcgtcttcat cgtggacctg ctgcagatgc tggagatgaa catggccatc
     1141 gccttccccg cagcgccctt gctgaccgtc atcctggctc tcgtcgggat ggaagccatc
     1201 atgtctgagt tcttcaatga taccaccacg gccttctaca tcatcctcac tgtgtggctg
     1261 gccgaccagt atgatgccat ctgctgccac accaacacca gcaagcggca ctggctgagg
     1321 ttcttctacc tctaccactt cgccttctat gcctaccact accgctttaa cgggcagtac
     1381 agcagcctgg ccctggtcac ctcctggctc ttcatccagc attccatgat ctacttcttc
     1441 caccactatg agttgcccgc catcctgcag cagatccgaa tccaggagat gctgcttcag
     1501 acgccaccgc tgggccccgg gacccccacg gcgctgcctg acgacctcaa caacaactct
     1561 ggctcccctg ccactccgga tcccagccct cccctcgcgc tgggccccag ctccagcccc
     1621 gcgcccactg gcggggcatc tgggcctggc tcactgggcg ctggggcctc agtatccggc
     1681 agtgacctag gttgggtggc cgagaccgcc gccatcatct ctgacgcatc cttcctgtcg
     1741 gggctgagcg cctctctcct ggagcggcgg ccaacagccc ctagtacccc ggacagctca
     1801 cgacctgacc ctggggtccc tctggaggac gcacccgccc ctgccgggtc ctgagaccgg
     1861 tgtcgtgccc ggctcccagt ggagccgcgg cccgagccca agagagctgt ggtctgcagg
     1921 gagaggggct ggtggcgaag gttctggaag cggccgggac agggcggcga tgggcacgag
     1981 gccatcggcc gcctggtgct ccccagtgcc ttccacacgg cgcccgcacg gggccgagcg
     2041 cccgggcccg gactagcagg tggtggctca cgatgcgggg cacacgttcc acgctattta
     2101 attgcagtgt acagagtcgt gtttgtatat agaataaact gtcgtggaca gtgaaaaaaa
     2161 aaaaaaaaaa
//
