LOCUS       NM_001163431             457 bp    mRNA    linear   ROD 15-DEC-2024
DEFINITION  Mus musculus transmembrane protein 258 (Tmem258), transcript
            variant 2, mRNA.
ACCESSION   NM_001163431
VERSION     NM_001163431.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Ramirez,A.S., Kowal,J. and Locher,K.P.
  TITLE     Cryo-electron microscopy structures of human
            oligosaccharyltransferase complexes OST-A and OST-B
  JOURNAL   Science 366 (6471), 1372-1375 (2019)
   PUBMED   31831667
REFERENCE   2  (bases 1 to 457)
  AUTHORS   Alvarez-Dominguez,J.R., Zhang,X. and Hu,W.
  TITLE     Widespread and dynamic translational control of red blood cell
            development
  JOURNAL   Blood 129 (5), 619-629 (2017)
   PUBMED   27899360
REFERENCE   3  (bases 1 to 457)
  AUTHORS   Graham,D.B., Lefkovith,A., Deelen,P., de Klein,N., Varma,M.,
            Boroughs,A., Desch,A.N., Ng,A.C.Y., Guzman,G., Schenone,M.,
            Petersen,C.P., Bhan,A.K., Rivas,M.A., Daly,M.J., Carr,S.A.,
            Wijmenga,C. and Xavier,R.J.
  TITLE     TMEM258 Is a Component of the Oligosaccharyltransferase Complex
            Controlling ER Stress and Intestinal Inflammation
  JOURNAL   Cell Rep 17 (11), 2955-2965 (2016)
   PUBMED   27974209
  REMARK    GeneRIF: we identify TMEM258 within this locus as a central
            regulator of intestinal inflammation. Strikingly, Tmem258
            haploinsufficient mice exhibit severe intestinal inflammation in a
            model of colitis.
REFERENCE   4  (bases 1 to 457)
  AUTHORS   Emoto,M., Miki,M., Sarker,A.H., Nakamura,T., Seki,Y., Seki,S. and
            Ikeda,S.
  TITLE     Structure and transcription promoter activity of mouse flap
            endonuclease 1 gene: alternative splicing and bidirectional
            promoter
  JOURNAL   Gene 357 (1), 47-54 (2005)
   PUBMED   15967598
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132148.3, BC058335.1 and AV456553.1.
            
            Transcript Variant: This variant (2) represents use of an alternate
            promoter and 5' UTR, and uses a downstream start codon, compared to
            variant 1. The resulting isoform (2) has a shorter N-terminus,
            compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BB826657.1, BC058335.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-73                AC132148.3         61202-61274
            74-441              BC058335.1         1-368
            442-457             AV456553.1         384-399
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 6.54 cM"
     gene            1..457
                     /gene="Tmem258"
                     /gene_synonym="1810006K21Rik"
                     /note="transmembrane protein 258"
                     /db_xref="GeneID:69038"
                     /db_xref="MGI:MGI:1916288"
     exon            1..92
                     /gene="Tmem258"
                     /gene_synonym="1810006K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            93..202
                     /gene="Tmem258"
                     /gene_synonym="1810006K21Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..329
                     /gene="Tmem258"
                     /gene_synonym="1810006K21Rik"
                     /note="isoform 2 is encoded by transcript variant 2;
                     UPF0197 transmembrane protein C11orf10 homolog;
                     oligosaccharyl transferase subunit TMEM258;
                     dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit TMEM258"
                     /codon_start=1
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit TMEM258 isoform 2"
                     /protein_id="NP_001156903.1"
                     /db_xref="CCDS:CCDS50386.1"
                     /db_xref="GeneID:69038"
                     /db_xref="MGI:MGI:1916288"
                     /translation="MSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRD
                     IYKELLISLVASLFMGFGVLFLLLWVGIYV"
     exon            203..339
                     /gene="Tmem258"
                     /gene_synonym="1810006K21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            340..447
                     /gene="Tmem258"
                     /gene_synonym="1810006K21Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      421..426
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem258"
                     /gene_synonym="1810006K21Rik"
                     /note="hexamer: AATAAA"
     polyA_site      441
                     /gene="Tmem258"
                     /gene_synonym="1810006K21Rik"
                     /note="major polyA site"
ORIGIN      
        1 gaccttgcct caagcatggc agaaggcgtc tctcagaaag ttgtcctcta acgtctaaga
       61 aaggagatga ggagtgacct gcaaaatggc aggagctcga agccatgagt aggtacacca
      121 gcccagtgaa cccggctgtc ttccctcacc tgactgtggt acttctggcc atcggcatgt
      181 tcttcactgc ctggttcttc gtttacgagg tcacctctac caagtacaca cgcgatattt
      241 acaaagagct cctcatctcc ttggtggcct cactcttcat gggctttgga gtcctcttcc
      301 tcctgctctg ggttggcatc tacgtataag tctccaaggg tacccaccag gcagctcacc
      361 aagtctctgc ttttgtaaat taatttttta ccattgctac gtgtcccacc tgctgtttac
      421 aataaaggca gatgtgtgac aggcctgaaa aaaaaaa
//
